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cDNA Library Construction of Testis and Ovary ofScylla serrata with SMART Technique - 贾锡伟; 王艺磊(集美大学水产学院水产生物技术研究所); 张子平(美国密歇根州立大学水产与野生生物学系); 李少菁
采用RDP试剂(改进自ChomczynskiandSacchi1987)提取锯缘青蟹精巢和卵巢的总RNA,经oligotex试剂盒纯化得到mRNA.利用SMART(switchingmechanismat5'endofRNAtranscript)技术,构建成精巢和卵巢的全长cDNA文库.在构建好的文库中,精巢的文库含有约1.45×106的重组子,卵巢的文库含有约为1.75×106的重组子.插入cDNA长度为500bp～3kb,说明两文库均具有良好的质量,为进一步筛选、克隆精巢与卵巢特异表达基因奠定了基础.
【英文摘要】 To clone the testis and ovary specific expression genes of Scylla serrata,we constructed two full-length cDNA libraries using the SMART cDNA library construction kit(Clontech).Total RNA were prepared from testes and ovaries using RDP,a reagent modified from Chomczynski and Sacchi(1987)for the simultaneous isolation of RNA,DNA,and protein.Oligotex(QIAGEN)was used to separate the mRNA of testes and ovaries from total RNA.The"anchor first-strand cDNA"containing asymmetricalSfiⅠrestriction enzyme sites(A&B) was...
(270769 bytes; application/pdf) - 31-dic-2008
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应用特征光合色素研究东海和南海北部浮游植物的群落结构 - 陈纪新; 黄邦钦; 刘媛; 曹振锐; 洪华生
应用高效液相色谱并结合二极管阵列检测器分析技术,研究了东海与南海北部典型断面浮游植物的光合色素组成,进而由CHEMTAX软件估算浮游植物群落结构。结果表明:东海断面浮游植物叶绿素a生物量大于南海北部断面。受海水层化结构影响,东海PN断面浮游植物群落结构形成上层和下层两种类型,上层以蓝藻为主要优势类群;下层以硅藻为主要优势类群。南海北部S断面浮游植物群落形成近岸与离岸两种类型,近岸以硅藻、隐藻、绿藻为主要优势类群;离岸以定鞭金藻、蓝藻、原绿球藻为主要优势类群。初步分析了研究海区浮游植物群落结构与环境因子的关系。
【英文摘要】 Phytoplankton samples from typical stations along the East China Sea and the northern south China sea were analyzed by RP-HPLC combined with DAD(diode array detector) to determine pigments composition.Ocean study included PN section along East China sea(September in 2002) and northern of south China sea(November in 2002).Pigments data were transformed into phytoplankton composition by software CHEMTAX based on least square method and steepest descent algorithm.This work showed that phytoplankton communities...
(634265 bytes; application/pdf) - 31-dic-2008
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三种体系萃取分离液闪测定海水中~(234)Th的比较 - 李鸿宾; 黄奕普; 陈敏; 刘广山; 邱雨生
本文对TOA萃取剂萃取钍适宜的操作条件及萃取溶剂进行了试验,提出了TOA/苯萃取234Th的最佳条件: 在 1cm3含 234Th的 6mol/dm3 HNO3 溶液中,用体积比为 0. 5的TOA/苯溶液 2cm3 萃取,然后用 2cm3 1. 0mol/dm3 H2SO4反萃取,最高萃取率达 94%.对TOA/苯、TBP/煤油和TTA/苯这 3种萃取体系的萃取效率及其影响因素、萃取机理、成本等做了比较,认为 3种体系各有利弊,但均可有效地应用于相关的海洋学研究中.
【英文摘要】 A new method was established that ()~(234)Th in seawater was separated and purified by the anion-exchange combined trioctylamine (TOA)/benzene extraction, and then determined by the ultra low level liquid scintillation spectrometer(LSS) and the best extraction conditions were found. 1 cm~3 6 mol/dm~3 HNO_3 solution containing ()~(234)Th was extracted by TOA/benzene (volume ratio equal 0.5) for 1 min, then back-extracted with 1.0 mol/dm~3 H_2SO_4 for 1 min. Adding Mg(NO_3)_2 or (Al(NO_3)_3) to solution extra...
(388217 bytes; application/pdf) - 31-dic-2008
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