Recursos de colección

Digital.CSIC (158.952 recursos)

Repositorio institucional del Consejo Superior de Investigaciones Científicas. Digital.CSIC es un depósito de documentos digitales, cuyo objetivo es organizar, archivar, preservar y difundir en modo de acceso abierto la producción intelectual resultante de la actividad investigadora del CSIC.

(CNB) Artículos post-print

Mostrando recursos 1 - 16 de 16

  1. Fungal Ku prevents permanent cell cycle arrest by suppressing DNA damage signaling at telomeres

    Sena-Tomás, Carmen de; Yu, Eun Young; Calzada, Arturo; Holloman, William K.; Lue, Neal F.; Pérez-Martín, José
    This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

  2. A novel molecular mechanism involved in multiple myeloma development revealed by targeting MafB to haematopoietic progenitors

    Vicente-Dueñas, Carolina; Romero-Camarero, Isabel; González-Herrero, Inés; Abollo-Jiménez, Fernando; Jiang, Xiaoyu; Gutiérrez, Norma Carmen; Orfao, Alberto; Pintado, Belén; Flores, Teresa; Rivas, Javier de las; Jiménez, Rafael; García-Criado, Francisco Javier; García-Cenador, Begoña; Lossos, Izidore S.; Cobaleda, César; Sánchez García, Isidro
    This article is licensed under a Creative Commons Attribution-NoncommercialNo DerivativeWorks 3.0 Licence.

  3. Genetic polymorphisms among C57BL/6 mouse inbred strains

    Zurita, Esther; Chagoyen, Mónica; Cantero, Marta; Alonso, Rosario; González-Neira, A.; López-Jiménez, A.; López-Moreno, J. A.; Landel, C. P.; Benítez, Javier; Pazos, Florencio; Montoliu, Lluís
    Mice from the inbred C57BL/6 strain have been commonly used for the generation and analysis of transgenic and knockout animal models. However, several C57BL/6 substrains exist, and these are genetically and phenotypically different. In addition, each of these substrains can be purchased from different animal providers and, in some cases, they have maintained their breeding stocks separated for a long time, allowing genetic differences to accumulate due to individual variability and genetic drift. With the aim of describing the differences in the genotype of several C57BL/6 substrains, we applied the Illumina® Mouse Medium Density Linkage Mapping panel, with 1,449 single...

  4. A fertilization-independent developmental program triggers partial fruit development and senescence processes in pistils of Arabidopsis

    Carbonell-Bejerano, Pablo; Úrbez, Cristina; Carbonell-Gisbert, Juan; Granell, Antonio; Pérez-Amador, Miguel A.
    Free via Open: Open Access articles can be viewed online without a subscription.

  5. Calcium influx through receptor-operated channel induces mitochondria-triggered paraptotic cell death

    Jambrina, Enrique; Alonso, Roberto; Alcalde, Marta; Rodríguez, María del Carmen; Serrano, Antonio; Martínez-A, Carlos; García-Sancho, Javier; Izquierdo, Manuel
    We address the specific role of cytoplasmic Ca2+ overload as a cell death trigger by expressing a receptor-operated specific Ca2+ channel, vanilloid receptor sub-type 1 (VR1), in Jurkat cells. Ca2+ uptake through the VR1 channel, but not capacitative Ca2+ influx stimulated by the muscarinic type 1 receptor, induced sustained intracellular [Ca2+] rises, exposure of phosphatidylserine, and cell death. Ca2+ influx was necessary and sufficient to induce mitochondrial damage, as assessed by opening of the permeability transition pore and collapse of the mitochondrial membrane potential. Ca2+induced cell death was inhibited by ruthenium red, protonophore carbonyl cyanide m-chlorophenylhydrazone, or cyclosporin A treatment,...

  6. Exacerbated inflammatory responses in transgenic mice expressing an inhibitor of apoptosis protein (OpIAP)

    Robles, Maria S.; Leonardo, Esther; Criado, Luis M.; Izquierdo, Manuel; Martínez-A, Carlos
    Members of the inhibitor of apoptosis protein family are involved not only in suppressing apoptosis, but also in signal transduction, cell division, and are associated with some types of cancers. Here we show that transgenic expression of the inhibitor of apoptosis protein OpIAP in murine T lymphocytes leads to a significant increase in T-cell receptor-induced cell activation, proliferation and cytokine production. Transgenic T lymphocytes expressing OpIAP have a lower proliferation threshold in response to T-cell receptor stimulation. Unstimulated OpIAP transgenic T lymphocytes show elevated nuclear levels of NF-κB transcription factor that increase after in vivo antigen peptide treatment. OpIAP transgenic...

  7. Inhibitor of apoptosis protein from ORGYIA pseudotsugata nuclear polyhedrosis virus provides a costimulatory signal required for optimal proliferation of developing thymocytes

    Robles, Maria S.; Leonardo, Esther; Criado, Luis M.; Izquierdo, Manuel; Martínez-A, Carlos
    The inhibitors of apoptosis proteins (IAPs) constitute a family of endogenous inhibitors that control apoptosis in the cell by inhibiting caspase processing and activity. IAPs are also implicated in cell division, cell cycle regulation, and cancer. To address the role of IAPs in thymus development and homeostasis, we generated transgenic mice expressing IAP generated from the baculovirus Orgyia pseudotsugata nuclear polyhedrosis virus (OpIAP). Developing thymocytes expressing OpIAP show increased nuclear levels of NF-κB and reduced cytoplasmic levels of its inhibitor, IκBα. In mature thymocytes, OpIAP induces optimal activation and proliferation after TCR triggering in the absence of a costimulatory signal....

  8. Degradation of cellular mRNA is a general early apoptosis-induced event

    Pret, M. Julieta del; Robles, Maria S.; Guío, Ana; Martínez-A, Carlos; Izquierdo, Manuel; García-Sanz, José A.
    El pdf del artículo es la versión post-print.

  9. Eukaryotic elongation factor 2 controls TNF-α translation in LPS-induced hepatitis

    Manieri, Elisa; Verdugo, Ángeles; González-Rodríguez, Águeda; Valverde, Ángela M.; Sabio, Guadalupe
    15 páginas, 7 figuras.-- et al.

  10. Diacylglycerol kinase α regulates the formation and polarisation of mature multivesicular bodies involved in the secretion of Fas ligand-containing exosomes in T lymphocytes

    Alonso, Roberto; Mazzeo, C.; Rodríguez, María del Carmen; Fraile-Ramos, A.; Calvo, Victor; Ávila-Flores, Antonia; Mérida, Isabel; Izquierdo, Manuel
    Multivesicular bodies (MVBs) are endocytic compartments that contain intraluminal vesicles formed by inward budding from the limiting membrane of endosomes. In T lymphocytes, these vesicles contain pro-apoptotic Fas ligand (FasL), which may be secreted as ‘lethal exosomes’ upon fusion of MVBs with the plasma membrane. Diacylglycerol kinase α (DGKα) regulate the secretion of exosomes, but it is unclear how this control is mediated. T-lymphocyte activation increases the number of MVBs that contain FasL. DGKα is recruited to MVBs and to exosomes in which it has a double function. DGKα kinase activity exerts a negative role in the formation of mature...

  11. Role of diacylglycerol kinase α in the attenuation of receptor signaling

    Sanjuán, Miguel Angel; Jones, David R.; Izquierdo, Manuel; Mérida, Isabel
    Diacylglycerol kinase (DGK) is suggested to attenuate diacylglycerol-induced cell responses through the phosphorylation of this second messenger to phosphatidic acid. Here, we show that DGKα, an isoform highly expressed in T lymphocytes, translocates from cytosol to the plasma membrane in response to two different receptors known to elicit T cell activation responses: an ectopically expressed muscarinic type I receptor and the endogenous T cell receptor. Translocation in response to receptor stimulation is rapid, transient, and requires calcium and tyrosine kinase activation. DGKα-mediated phosphatidic acid generation allows dissociation of the enzyme from the plasma membrane and return to the cytosol, as...

  12. Diacylglycerol kinase α regulates the secretion of lethal exosomes bearing fas ligand during activation-induced cell death of T lymphocytes

    Alonso, Roberto; Rodríguez, María del Carmen; Pindado, José; Merino Plaza, Ernesto; Mérida, Isabel; Izquierdo, Manuel
    Fas ligand (FasL) mediates both apoptotic and inflammatory responses in the immune system. FasL function critically depends on the different forms of FasL; soluble Fas ligand lacking the transmembrane and cytoplasmic domains is a poor mediator of apoptosis, whereas fulllength, membrane-associated FasL (mFasL) is pro-apoptotic. mFasL can be released from T lymphocytes, via the secretion of mFasL-bearing exosomes. mFasL in exosomes retains its activity in triggering Fas-dependent apoptosis, providing an alternative mechanism of cell death that does not necessarily imply cell-to-cell contact. Diacylglycerol kinase α (DGKα), a diacylglycerol (DAG)-consuming enzyme, is involved in the attenuation of DAG-derived responses initiated at...

  13. A new role of diacylglycerol kinase α on the secretion of lethal exosomes bearing Fas ligand during activation-induced cell death of T lymphocytes

    Alonso, Roberto; Mazzeo, C.; Mérida, Isabel; Izquierdo, Manuel
    Exosomes are small membrane vesicles that intracellularly accumulate into late or multivesicular endosomes (multivesicular bodies, MVB). Exosomes have a particular lipid and protein content, reflecting their origin as intraluminal vesicles of late endosomes. The stimulation of several hematopoietic cells induces the fusion of the limiting membrane of the MVB with the plasma membrane, leading to the release of exosomes towards the extracellular environment. In T lymphocytes, stimulation of the T cell receptor (TCR) induces the fusion of the MVBs with the plasma membrane and exosomes carrying several bio-active proteins are secreted. Among these proteins, the pro-apoptotic protein Fas ligand (FasL)...

  14. Ethylene is involved in pistil fate by modulating the onset of ovule senescence and the GA-mediated fruit set in Arabidopsis

    Carbonell-Bejerano, Pablo; Úrbez, Cristina; Granell, Antonio; Carbonell-Gisbert, Juan; Pérez-Amador, Miguel A.
    This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

  15. Identification of genes specifically expressed in cauliflower reproductive meristems. Molecular characterization of BoREM1

    Franco-Zorrilla, José Manuel; Fernández-Calvín, Begoña; Madueño Albi, Francisco; Cruz-Álvarez, Marilyn; Salinas, Julio; Martínez-Zapater, José M.
    Using the meristems of the cauliflower curd as a source of tissue and a series of subtractive hybridizations and amplification reactions, we have constructed a cDNA library highly enriched in cDNAs expressed in reproductive meristems. The analysis of a sample of 250 clones from this library identified 22 cDNA clones corresponding to genes specifically expressed in these cauliflower meristems. Apart from two clones that corresponded to APETALA1, and two other ones showing similarity to different aminoacyl-tRNA synthetases, the remaining clones showed no similarity to any sequence in the databases and may correspond to novel genes. One of these clones, BoREM1,...

  16. Dimerization of Arabidopsis 14-3-3 proteins: structural requirements within the N-terminal domain and effect of calcium

    Abarca, Dolores; Madueño Albi, Francisco; Martínez-Zapater, José M.; Salinas, Julio
    The structural requirements for dimerization of RCI14A and RCI14B, two 14-3-3 isoforms from Arabidopsis thaliana, have been analyzed by testing truncated forms of RCI14A for dimerization with full-length RCI14A and RCI14B. The results show that only the fourth helix of the truncated partner is essential for dimerization, which represents a difference from what is known for animal isoforms. On the other hand, the effect of calcium has been tested in RCI14A homodimerization. Millimolar concentrations of calcium exert a negative, dose-dependent effect that involves the C-terminal domain of RCI14A and might modulate interactions with other cellular components or among Arabidopsis 14-3-3...

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