Recursos de colección

Digital.CSIC (162.855 recursos)

Repositorio institucional del Consejo Superior de Investigaciones Científicas. Digital.CSIC es un depósito de documentos digitales, cuyo objetivo es organizar, archivar, preservar y difundir en modo de acceso abierto la producción intelectual resultante de la actividad investigadora del CSIC.

(IBV) Artículos post-print

Mostrando recursos 1 - 6 de 6

  1. Role of an intrasubunit disulfide in the association state of the cytosolic homo-oligomer methionine adenosyltransferase

    Sánchez-Pérez, Gabino F.; Gasset, M.; Calvete, Juan J.; Pajares, María A.
    Recombinant rat liver methionine adenosyltransferase has been refolded into fully active tetramers (MAT I) and dimers (MAT III), using as a source chaotrope-solubilized aggregates resulting from specific washes of inclusion bodies. The conditions of refolding, dialysis in the presence of 10 mM dithiothreitol or 10 mM GSH with I mM GSSG, allowed the production of both isoforms, the nature of the redox agent determining the capacity of the final product (MAT I/III) to interconvert. Refolding in the presence of 10 mM dithiothreitol yielded mainly MAT III in a concentration-dependent equilibrium with the homotetramer MAT I. However, refolding in the presence...

  2. Role of an intrasubunit disulfide in the association state of the cytosolic homo-oligomer methionine adenosyltransferase

    Sánchez-Pérez, Gabino F.; Gasset, M.; Calvete, Juan J.; Pajares, María A.
    Recombinant rat liver methionine adenosyltransferase has been refolded into fully active tetramers (MAT I) and dimers (MAT III), using as a source chaotrope-solubilized aggregates resulting from specific washes of inclusion bodies. The conditions of refolding, dialysis in the presence of 10 mM dithiothreitol or 10 mM GSH with I mM GSSG, allowed the production of both isoforms, the nature of the redox agent determining the capacity of the final product (MAT I/III) to interconvert. Refolding in the presence of 10 mM dithiothreitol yielded mainly MAT III in a concentration-dependent equilibrium with the homotetramer MAT I. However, refolding in the presence...

  3. Role of an intrasubunit disulfide in the association state of the cytosolic homo-oligomer methionine adenosyltransferase

    Sánchez-Pérez, Gabino F.; Gasset, M.; Calvete, Juan J.; Pajares, María A.
    Recombinant rat liver methionine adenosyltransferase has been refolded into fully active tetramers (MAT I) and dimers (MAT III), using as a source chaotrope-solubilized aggregates resulting from specific washes of inclusion bodies. The conditions of refolding, dialysis in the presence of 10 mM dithiothreitol or 10 mM GSH with I mM GSSG, allowed the production of both isoforms, the nature of the redox agent determining the capacity of the final product (MAT I/III) to interconvert. Refolding in the presence of 10 mM dithiothreitol yielded mainly MAT III in a concentration-dependent equilibrium with the homotetramer MAT I. However, refolding in the presence...

  4. Hepatic insulin resistance is associated with increased apoptosis and fibrogenesis in nonalcoholic steatohepatitis and chronic hepatitis C

    García-Monzón, Carmelo; Mayoral, Rafael; González-Rodríguez, Águeda; Casado, Marta; Boscá, Lisardo; Valverde, Ángela M.; Martín-Sanz, Paloma
    [Background and Aims]: We aimed to elucidate whether hepatic insulin resistance may contribute to hepatocyte apoptosis and fibrogenesis in nonalcoholic fatty liver disease (NAFLD) and in chronic hepatitis C virus (HCV) infection. [Methods]: Twenty-seven nonalcoholic steatosis (NAST), 24 nonalcoholic steatohepatitis (NASH), 71 HCV, and 29 patients with histological normal liver (NL) were studied. Real-time PCR, the TUNEL assay, and Western blots were used to assess insulin-signaling molecules, hepatocyte apoptosis, antiapoptotic mediators, active caspase 3, and type I collagen in liver biopsies. HCV core-transfected human hepatocytes were used as an in vitro model. [Results]: In NAFLD patients, hepatic levels of insulin...

  5. Cyclooxygenase-2 over-expression inhibits liver apoptosis induced by hyperglycemia

    Francés, Daniel E.; Mayoral, Rafael; Través, Paqui G.; Casado, Marta; Valverde, Ángela M.; Martín-Sanz, Paloma; Carnovale, Cristina E.
    Increased expression of COX-2 has been linked to inflammation and carcinogenesis. Constitutive expression of COX-2 protects hepatocytes from several pro-apoptotic stimuli. Increased hepatic apoptosis has been observed in experimental models of diabetes. Our present aim was to analyze the role of COX-2 as a regulator of apoptosis in diabetic mouse liver. Mice of C57BL/6 strain wild type (Wt) and transgenic in COX-2 (hCOX-2 Tg) were separated into Control (vehicle) and SID (streptozotocin induced diabetes, 200 mg/kg body weight, i.p.). Seven days post-injection, Wt diabetic animals showed a decrease in PI3K activity and P-Akt levels, an increase of P-JNK, P-p38, pro-apoptotic...

  6. Proteomic analysis of phosphorylated nuclear proteins underscores novel roles for rapid actions of retinoic acid in the regulation of mRNA splicing and translation

    Laserna Mendieta, Emilio J.; Valero, María Luz; Sanz, Libia; Sánchez del Pino, Manuel M.; Calvete, Juan J.; Barettino, Domingo
    16 pages, 8 figures, 6 tables.- PMID: 19812389 [PubMed]

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