Recursos de colección

Digital.CSIC (162.855 recursos)

Repositorio institucional del Consejo Superior de Investigaciones Científicas. Digital.CSIC es un depósito de documentos digitales, cuyo objetivo es organizar, archivar, preservar y difundir en modo de acceso abierto la producción intelectual resultante de la actividad investigadora del CSIC.

(IIB) Artículos post-print

Mostrando recursos 1 - 20 de 103

  1. Neurogenic potential assessment and pharmacological characterization of 6-Methoxy-1,2,3,4-tetrahydro-β-carboline (Pinoline) and Melatonin-Pinoline Hybrids

    Fuente Revenga, Mario de la; Pérez, Concepción; Morales-García, José A.; Alonso-Gil, Sandra; Pérez Castillo, Ana; Yáñez, Matilde; Rodríguez-Franco, María Isabel
    This work received the honour of being the Cover of the ACS Chemical Neuroscience in its issue of May 2015

  2. MicroRNA deep-sequencing reveals master regulators of follicular and papillary thyroid tumors

    Mancikova, Veronika; Matías-Guiu, Xavier; Riesco-Eizaguirre, Garcilaso; Santisteban, Pilar; Robledo, Mercedes
    et al.

  3. Gold nanoparticles functionalised with fast water exchanging Gd3+ chelates: linker effects on the relaxivity

    Ferreira, Miguel F.; Calle, Daniel; López-Larrubia, Pilar; Cerdán, Sebastián; Rodrigues, Tiago B.; Helm, Lothar; Martins, José A.; Geraldes, Carlos F. G. C.
    et al.

  4. Endogenous NAMPT dampens chemokine expression and apoptotic responses in stressed tubular cells

    Ucero, Alvaro C.; Benito-Martin, Alberto; Santamaria, Beatriz; Ruiz-Ortega, Marta; Ortiz, Alberto
    et al.

  5. TNF-related weak inducer of apoptosis (TWEAK) promotes kidney fibrosis and Ras-dependent proliferation of cultured renal fibroblast

    Ucero, Alvaro C.; Santamaria, Beatriz; López-Novoa, José M.; Ruiz-Ortega, Marta
    et al.

  6. Topical administration of somatostatin prevents retinal neurodegeneration in experimental diabetes

    Hernández, Cristina; García-Ramírez, Marta; González-Rodríguez, Águeda; Valverde, Ángela M.; Simó, Rafael
    Under License Creative Commons.-- et al.

  7. Insulin directly stimulates VEGF-A production in the glomerular podocyte

    Santamaria, Beatriz; Valverde, Ángela M.; Coward, R. J.
    et al.

  8. Cloning and developmental expression of Sna, a murine homologue of the Drosophila snail gene

    Nieto, M. Ángela; Bennett, Michael F.; Sargent, Michael G.; Wilkinson, David G.
    11 páginas, 6 figuras.

  9. Conserved segmental expression of Krox-20 in the vertebrate hindbrain and its relationship to lineage restriction

    Nieto, M. Ángela; Bradley, Leila C.; Wilkinson, David G.
    6 páginas, 4 figuras.

  10. Direct drug application to the round window: A comparative study of ototoxicity in rats

    Murillo-Cuesta, Silvia; Camarero, Guadalupe; Varela-Nieto, Isabel; Rivera, Teresa
    [Objective]: To assess the validity of inducing ototoxicity in rats by applying a sponge soaked in kanamycin and furosemide on the round window. [Study Design]: Basic, randomized, nonblind experimental study. [Setting]: Animal models of cochlear damage and reliable methods of local drug delivery are fundamental to study hearing loss and to design new therapies. [Subjects and Methods]: Four experimental groups of six Wistar rats with different methods of drug administration were used: (1) injection of subcutaneous kanamycin (400 mg/kg) and intravenous furosemide (100 mg/kg); (2) local application of a sponge soaked in saline close to the round window; (3) animals...

  11. Effects of thyroid hormone replacement on associative learning and hippocampal synaptic plasticity in adult hypothyroid rats

    Fernández-Lamo, Iván; Montero-Pedrazuela, Ana; Delgado-García, José María; Guadaño-Ferraz, Ana; Gruart, Agnès
    Activity-dependent changes taking place at the hippocampal perforant pathway–dentate gyrus synapse during classical eyeblink conditioning were recorded in adult thyroidectomized (hypothyroid) and control (euthyroid) rats, and in animals treated with thyroid hormones 20 days after thyroidectomy (recovery rats). The aim was to determine the contribution of thyroid hormones and the consequences of adult-onset hypothyroidism to both associative learning and the physiological potentiation of hippocampal synapses during the actual learning process in alert behaving animals. Control and recovery rats presented similar learning curves, whereas hypothyroid animals presented lower values. A single pulse presented to the perforant pathway during the conditioned–unconditioned inter-stimulus...

  12. Genomic organization and control of the Grb7 gene family

    Lucas, Elisa; García-Palmero, Irene; Villalobo, Antonio
    Grb7 and their related family members Grb10 and Grb14 are adaptor proteins, which participate in the functionality of multiple signal transduction pathways under the control of a variety of activated tyrosine kinase receptors and other tyrosine-phosphorylated proteins. They are involved in the modulation of important cellular and organismal functions such as cell migration, cell proliferation, apoptosis, gene expression, protein degradation, protein phosphorylation, angiogenesis, embryonic development and metabolic control. In this short review we shall describe the organization of the genes encoding the Grb7 protein family, their transcriptional products and the regulatory mechanisms implicated in the control of their expression. Finally,...

  13. Loss of protein tyrosine phosphatase 1B increases IGF-I receptor tyrosine phosphorylation but does not rescue retinal defects in IRS2-deficient mice

    Arroba, Ana I.; Revuelta-Cervantes, Jesús; González-Rodríguez, Águeda; Pardo, Virginia; Burks, Deborah J.; Valverde, Ángela M.
    et al.

  14. Two new patients bearing mutations in the fukutin gene confirm the relevance of this gene in Walker-Warburg syndrome

    Cotarelo, R. P.; Martínez-Frías, María Luisa; Cruces, Jesús
    Instituto de Salud Carlos III (España)

  15. Snail silencing effectively suppresses tumour growth and invasiveness

    Olmeda, David; Jordá, María; Peinado, Héctor; Fabra, Àngels; Cano, Amparo
    The transcription factor Snail has been recently proposed as an important mediator of tumour invasion because of its role in downregulation of E-cadherin and induction of epithelial-mesenchymal transitions (EMT). This behaviour has led to the consideration of Snail as a potential therapeutic target to block tumour progression. In this report, we provide evidence for this hypothesis. We show that silencing of Snail by stable RNA interference in MDCK-Snail cells induces a complete mesenchymal to epithelial transition (MET), associated to the upregulation of E-cadherin, downregulation of mesenchymal markers and inhibition of invasion. More importantly, stable interference of endogenous Snail in two...

  16. Levels of protein tyrosine phosphatase 1B determine susceptibility to apoptosis in serum-deprived hepatocytes

    González-Rodríguez, Águeda; Escribano, Óscar; Alba, Javier; Rondinone, Cristina M.; Benito, Manuel; Valverde, Ángela M.
    Protein tyrosine phosphatase 1B (PTP1B) is a negative regulator of tyrosine kinase growth factor signaling. To assess the importance of PTP1B in the balance between death and survival in the liver, we have developed immortalized neonatal hepatocyte cell lines lacking (PTP1B-/-) or overexpressing (PTP1B+/+PTP1B) PTP1B. Early activation of caspase-3 occurred in PTP1B+/+PTP1B hepatocytes but was nearly abolished in PTP1B -/- cells. At the molecular level, PTP1B overexpression/deficiency altered the balance of pro-(Bim) and anti-(Bcl-xL) apoptotic members of the Bcl-2 fmily upon serum withdrawal. Likewise, cytosolic cytochrome C increased rapidly in PTP1B+/+PTP1B hepatocytes whereas it was retained in the mitochondria of...

  17. Protein tyrosine phosphatase 1B modulates GSK3b/Nrf2 and IGFIR signaling pathways in acetaminophen-induced hepatotoxicity

    Mobasher, Maysa A.; González-Rodríguez, Águeda; Santamaria, Beatriz; Ramos, Sonia; Martín, M. Ángeles; Goya, Luis; Rada, Patricia; Cuadrado, Antonio; Martín-Pérez, Jorge; Valverde, Ángela M.
    This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License.-- et al.

  18. Protein-tyrosine phosphatase 1B-deficient myocytes show increased insulin sensitivity and protection against tumor necrosis factor-α-induced insulin resistance

    Nieto-Vázquez, Iria; Fernández-Veledo, Sonia; Rondinone, Cristina M.; Valverde, Ángela M.; Lorenzo, Margarita
    Protein-tyrosine phosphatase (PTP)1B is a negative regulator of insulin signaling and a therapeutic target for type 2 diabetes. In this study, we have assessed the role of PTP1B in the insulin sensitivity of skeletal muscle under physiological and insulin-resistant conditions. Immortalized myocytes have been generated from PTP1B-deficient and wild-type neonatal mice. PTP1B-/- myocytes showed enhanced insulin-dependent activation of insulin receptor autophosphorylation and downstream signaling (tyrosine phosphorylation of insulin receptor substrate [IRS]-1 and IRS-2, activation of phosphatidylinositol 3-kinase, and serine phosphorylation of AKT), compared with wild-type cells. Accordingly, PTP1B-/- myocytes displayed higher insulin-dependent stimulation of glucose uptake and GLUT4 translocation to...

  19. Role of insulin receptor in the regulation of glucose uptake in neonatal hepatocytes

    Nevado, Carmen; Valverde, Ángela M.; Benito, Manuel
    The liver plays a major role in the regulation of glucose homoeostasis. Evidence from liver-specific insulin receptor knockout mice (LIRKO) suggested that insulin's direct and indirect effects on glucose utilization by the liver both require the presence of hepatic insulin receptors (IR). To address this issue, we have generated immortalized neonatal hepatocytes bearing (HIR LoxP) or not (HIR KO) IR. The lack of IR significantly decreased basal glucose uptake in neonatal hepatocytes from 3- to 14-d-old mice, and the expression of glucose transporter 1 (GLUT1), GLUT2, and glucokinase (GK) remained unchanged throughout development. HIR KO reconstituted hepatocytes with IRA but...

  20. RC3/neurogranin is expressed in pyramidal neurons of motor and somatosensory cortex in normal and denervated monkeys

    Guadaño-Ferraz, Ana; Bernal, Juan; Rausell, Estrella
    RC3/neurogranin is a neuron-specific calpacitin located in the cytoplasm and, especially, in dendrites and dendritic spines of cortical neurons, involved in many aspects of excitatory transmission and long-term potentiation. We investigated RC3 expression in pyramidal cortical neurons and interneurons of the motor and somatosensory cortex of normal Macaca fascicularis by means of double immunofluorescence and with techniques that combine immunohistochemistry and radioactive in situ hybridization. We show that RC3 is expressed in virtually all pyramidal neurons and spiny stellate neurons of neocortical areas 4, 3b, 1, 2, 5, 7, and SII, but not in the majority of cortical interneurons. RC3...

Aviso de cookies: Usamos cookies propias y de terceros para mejorar nuestros servicios, para análisis estadístico y para mostrarle publicidad. Si continua navegando consideramos que acepta su uso en los términos establecidos en la Política de cookies.