Repositorio institucional del Consejo Superior de Investigaciones Científicas.
Digital.CSIC es un depósito de documentos digitales, cuyo objetivo es organizar, archivar, preservar y difundir en modo de acceso abierto la producción intelectual resultante de la actividad investigadora del CSIC.
(IRN) Artículos post-print
Mostrando recursos 1 - 20 de 106
Preparados para sobrevivir (Ciencia y desarrollo ecología y desarrollo) - Pastor Piñeiro, Jesús
El desarrollo de la ciencia y
de la técnica de una sociedad
es parte de su avance
cultural, y en una sociedad
moderna es parte central
de dicho desarrollo.
Por tanto, el progreso
científico y técnico no puede darse con
independencia del desarrollo cultural,
lo que a su vez no puede producirse sin
un desarrollo económico y político paralelo.
Es un hecho que, aunque puede haber
investigaciones originales y valiosas
en condiciones políticas y económicas
duras, no puede existir un desarrollo
científico y técnico integral y sostenido,
a menos que exista libertad política,
tolerancia intelectual y cierta holgura
económica. En suma, el desarrollo
científico-técnico y económico requiere
el avance de la sociedad en todos sus
Molecular and functional analysis of α-amylase inhibitor genes and proteins in the common bean Phaseolus vulgaris - Mirkov, T. E.; Pueyo, José J.; Mayer, J. E.; Kjemtrup, S.; Cardona, C.; Chrispeels, Maarten J.
Bean (Phaseolus vulgaris) seeds accumulate three evolutionarily related plant defense proteins: phytohemaglutinin (PHA), arcelin (Arc) and α-amylase inhibitor (αAI). we identified the gene that encodes snap bean(cv. Greensleeves) αAI on the basis of a partial amino acid sequence of purified αAI supplied by John Whitaker (UC Davis), and the expression of the gene in tobacco seeds.
Independent induction of two blue light-dependent monovalent anion transport systems in the plasma membrane of Monoraphidium braunii - Mora, Cristina; Witt, Federico G.; Aparicio, Pedro J.; Quiñones, Miguel Angel
In the plasma membrane of the green alga Monoraphidium braunii there are at least two monovalent anion transport systems. One of them is specific for bicarbonate. This transport system is activated by blue light and its induction is triggered by a decrease in the external CO2 concentration. The second transport system is responsible for nitrate uptake at least. This transport system is also activated by blue light and its induction occurs when there is no ammonium in the external medium. Both transport systems are synthesized independently. Hence, when M. braunii cells grow with nitrate as the only nitrogen source under...
La revegetación de vertederos de residuos urbanos basada en principios ecológicos - Hernández, A. J.; Pastor Piñeiro, Jesús
El mayor nivel de degradación de ecosistemas terrestres se da en aquellos casos en los que la
misma es debida a la interacción erosión-contaminación. En la situación actual, los vertederos de
residuos sólidos urbanos (VRSU) que son clausurados nos permiten tener una visión adecuada para la
investigación acerca de la rehabilitación de dichos ecosistemas. Sin duda, el proceso de la revegetación
ha sido considerado como primario a fin de conseguir nuestros objetivos.
La metodología ha consistido en estudiar comparativamente (91 estaciones de muestreo) tres
vertederos con presencia de erosión-contaminación y los ecosistemas del entorno que tuvieron
abundancia en especies herbáceas, ya que las primeras etapas de la...
Nutrient recycling as an ecological basis for the technologies involved in a sustainable development in semiarid mediterranean rangelands - Pastor Piñeiro, Jesús; Hernández, A. J.; Bello, A.
Recycling is the central axis for the conservaron of ecosystems ¡n the scenario of
sustainable development. This subject implies the study of an important frame of interactions.
Nevertheless, the complex study of nutrient recycling in agroecosystems would
be reduced on the basis of the actual knowledge of the soil biocenoses, mainly represented
by soil nematodes.
Nematodes are indicator organisms of several processes related with the trophic
structure of soil systems. This question, combined with the study of nematode interactions
with the herbaceous communities growing over these soils, can provide guidelines
for a more rational planning of pasture management.
The results of our work show that nematode density is posrtively...
In situ microscopical diagnosis of biodeterioration processes at the convent of Santa Cruz la Real, Segovia, Spain - Ríos, Asunción de los; Galván, Virginia; Ascaso, Carmen
This report describes the in situ evaluation of biodeterioration processes occurring in the stone (granodiorite and carbonate) of the
Convento de Santa Cruz la Real, Segovia, Spain. Scanning electron microscopy with back-scattered electron imaging (SEM–BSE) and
11 X-ray microanalysis were used to examine stone samples from the convent. Free-living algae and fungi, lichen thalli, mosses, cyanobacteria
and bacterial colonies were observed. Some of these organisms were located epilithically, while others were found to inhabit ssures
13 or cavities inside the lithic substrate, a few of which showed an euendolithic ecological niche. Biogeomechanical and biogeochemical
processes associated with the presence and action of lithobiontic microorganisms were...
The effect of desiccation on cell shape in the lichen Parmelia Sulcata taylor - Brown, D. H.; Rapsch, S.; Beckett, A.; Ascaso, Carmen
Comparisons have been made of the shape of algal cells in the lichen Parmelia sulcata which
was subjected to controlled desiccation regimes inducing substantial water loss. The spherical
appearance of algal cells obtaiiíed by conventional techniques for transmission electrón
microscopy (TEM) was shown by low temperature scanning electrón microscopy (LTSEM)
to be the consequence of rapid rehydration during fixation. Collapse of the walls of algal cells
and fungal hyphae in the medulla and algal layer when desiccated were observed with LTSEM
and shown to be reversible on rehydration. Desiccation-induced contraction of cortical fungal
protoplasts was detected with LTSEM. Fixation with osmium vapour only before TEM
demonstrated a similar...
Temporal variations in the carbon budget of forest ecosystems in Spain - Rodríguez Murillo, Juan Carlos
Temperate and boreal forests of the Northern Hemisphere have recently been
identified as important carbon sinks. Accurate calculation of forest carbon budget and
appraisal of the temporal variations of forest net carbon fluxes are important topics to
elucidate the ‘‘missing sink’’ question and to follow up the changing carbon dynamics in
In this article, recent carbon budgets of the forests of a region in northern Spain have
been calculated using data from forest inventories and a carbon cycle model for the human
perturbations to the forests. Two methods are used, one that requires data from two forest
inventories (growing stocks), and another that uses data from only...
Sedimentation in the semi-arid freshwater wetland Las Tablas de Daimiel (Spain) - Sánchez Carrillo, Salvador; Álvarez Cobelas, Miguel; Angeler, D. G.
Despite sedimentation being recognized as a very important process in wetlands, very little is known about its dynamics. This study analyzed sedimentation on a short-term scale in the semiarid floodplain wetland Las Tablas de Daimiel National Park (central Spain). In monthly sampling intervals during 1997 and 1998, we measured seasonal and spatial sedimentation patterns of total solids, organic and mineral matter, nitrogen, and phosphorus using sediment traps. The highest mean mass accumulation rates were obtained at the wetland-input site (135 ± 57 g m−2 d−1), but a decrease in sedimentation rates was noted with increasing distance from the inflow (49...
Cultivation-Induced Effects on the Organic Matter in Degraded Southern African Soils - Pardo, Mª Teresa; Almendros, G.; Zancada, M. C.; López-Fando, Cristina; González-Vila, F.J.
We studied quantitative and qualitative changes in soil organic matter (SOM) due to
different land uses (reference woodland versus cultivated) on six soils from Tanzania
(Mkindo and Mafiga), Zimbabwe (Domboshawa and Chickwaka), and South Africa
(Hertzog and Guquka). Structural characteristics of the humic acids (HAs) were measured
by Curie-point pyrolysis–gas chromatography/mass spectrometry (Py–GC/MS)
and solid-state 13C nuclear magnetic resonance (CPMAS 13C NMR) spectroscopy.
Significant changes in concentration and composition of SOM were observed between
land uses. Losses of organic carbon after cultivation ranged from 35% to 50%. Virgin
soils showed large proportions of colloidal humus fractions: humic acids (HAs) and
fulvic acids (FAs) but negligible amounts of not-yet decomposed...
Mutants of coliphage BF23 able to propagate on smooth strains of Salmonella Typhimurium - Mojica-A, T.; García, E.; Ascaso, Carmen
Mutants of coliphage BF23 able to propágate on smooth strains of
The host range of a bacteriophage can be modified by mutation
(and/or recombination) either of the host and/or of the bacteriophage.
We have previously reported (MOJICA-A & CHARLES, 1975)
that coliphage BF23 was able to propágate on rough strains (mutation
of the host) of Salmonella typhimurium (strains with incomplete
lipopolysaccharide of the cell wall), and that the sensitivity of
rough strains was dependent on the presence of an intact bfe+
gene. Moreover the inability of the phage to propágate on smooth
strains was due to failure of the phage to absorb to the bacterial
Unmutagenized stocks of phage...
Leguminosas y gramineas como base para la tipificación de pastos. II comunidades de caracter subhúmedo y xerofítico - Pastor Piñeiro, Jesús; Oliver, Salvador; Gómez Sal, A.
Using only legume and grass species, we pretend to characterize a group of pastures,
subhumid and xerophytic by means of an ordination analysis. This analysis
differentiates clearly the communities with mesomediterranean bioclimatic characteristics
from those with supramediterranean ones.
The transition between both bioclimatic environments is defined by the "tomillar-
pasto" (al. Brachypodion phoenicoidé) and 'lastonares" (al. Festuco-Poion
ligulatae) according to topographic and soil characteristics.
Given the complex character of these subhumid pastures, in the southem limit
of their distribution área, the use of only these species belonging to the above mentioned
families, is not sufficient to differentiate the main types of communities
described for the studied área, although it does...
Boron, Zinc, Iron, and Manganese Content in Four Grassland Species - Adarve, M. J.; Hernández, A. J.; Gil, A.; Pastor Piñeiro, Jesús
A pot experiment was carríed oul to test the response of the B,
Zn, Fe, and Mn concentraron in four wild herbaceous specics exposcd
to three landñll leachate treatments of increasing concentration of
contaminants. The species tested «ere clustered clover (Trifolium
(¡lomera tum L.), col ton clover (T. tomentosum L.), wall barley
(Hordeum murinum L.), and soft brome (Bromus hordaceus L.). The
legume specics accumulated more Fe and B than the grasses. The
least contaminated leachate (leachate A) significantly increased the
Fe and Mn content in T. glomeratum. Leachate B significantly increased
the Zn content in both clover species and Fe content in T.
glomeratum and H. murinum, while it...
Efecto de diferentes tipos de uso sobre los componentes edáfico y biótico de agroecosistemas de secano - Hernández, A. J.; Pastor Piñeiro, Jesús; Estalrich, E.; Urcelay, A.; Lacasta Dutoit, Carlos
Los principales problemas ambientales asociados con las prácticas cerealistas
en la submeseta sur son la evolución negativa de las características edáficas y su repercusión
en los procesos erosivos. En esta región semiárida interactúan el clima, la topografía, el suelo y la
cubierta vegetal para determinar el grado de degradación del medio. Desde este punto de vista, el
objetivo de este estudio ha sido examinar los componentes edáfico y biológico de los agroecosistemas
subsiguientes a la retirada del cultivo cerealista, en los que la cantidad y el tipo de la cubierta
vegetal son los que controlan la protección del suelo. Como consecuencia de ello la vegetación es
Cubiertas vegetales en olivar y viñedo: balance de diez años en relación al agua del suelo y su monitorización - Pastor Piñeiro, Jesús; Benítez, M.; Hernández, A. J.
Plant covers in olive grove and vineyard: ten years balance in relation to soil water and its monitoring
The conceptual framework affects necessary ecológica! engineering for the sustainability of woody cultivations
in semiarid Mediterranean environments and tries to articúlate the management of several components of these
ecological agroecosystems to optimize soil water without covers irrigation.
This report describes ways of managing plant covers ("weeds" or native legumes) in relation to the prevailing
rainfall régimen. Over the past 10 years, we have been monitoring soil moisture levéis in an extensive olive
plantation and vineyard at different depths under the different plant canopies through standard procedures
(gravimetric followed by...
Preparation and properties of a cross-linked complex between ferredoxin - NADP+ reductase and flavodoxin - Pueyo, José J.; Sancho, Javier; Edmondson, Dale E.; Gómez-Moreno, Carlos
The electrostatically stabilized complex between Anahaena variabilis ferredoxin - NADP' reductase and
Azotobacter vinelandii flavodoxin has been covalently cross-linked by treatment with l-ethyl-3-(3-dimethylaminopropy1)
carbodiimide. The covalent complex exhibits a molecular mass and FMN/FAD content consistent with
that expected for a 1 : 1 stoichiometry of the two flavoproteins. lmmunochemical cross-reactivity is exhibited by the
covalent complex with rabbit antisera prepared separately against each protein. The complex retains NADPH -
ferricyanide diaphorase activity although the K,,, for ferricyanide is increased twofold and the turnover number
is decreased by a factor of two when compared to native reductase. NADPH -cytochrome-c reductase activity
of the complex is observed at a...
An unusual infection mechanism and nodule morphogenesis in white lupin (Lupinus albus) - González-Sama, Alfonso; Lucas, M. Mercedes; Felipe, Mª Rosario de; Pueyo, José J.
The infection of white lupin (
) roots and the early stages in organogenesis
of the lupinoid nodule are characterized in detail in this work.
• Immunolabelling of
) ISLU16 and green fluorescent
protein labelling of
NZP2037, two strains that induce nodulation
, allowed us to monitor the infection and morphogenesis process.
Light and transmission electron microscopy, low-temperature scanning electron
microscopy, fluorescence and confocal microscopy were employed.
• Rhizobia penetrated the root intercellularly at the junction between the root hair
base and an adjacent epidermal cell. Bacteria invaded the subepidermal cortical cell
immediately beneath the root hair through structurally altered cell wall regions. The
newly infected cell divided repeatedly...
Legume nodule senescence: roles for redox and hormone signalling in the orchestration of the natural aging process - Puppo, Alain; Groten, Karin; Bastian, Fabiola; Carzaniga, Raffaella; Soussi, Mariam; Lucas, M. Mercedes; Felipe, Mª Rosario de; Harrison, Judith; Vanacker, Helene; Foyer, Christine H.
Research on legume nodule development has contributed greatly to our current
understanding of plant–microbe interactions. However, the factors that orchestrate
root nodule senescence have received relatively little attention. Accumulating
evidence suggests that redox signals contribute to the establishment of symbiosis
and senescence. Although degenerative in nature, nodule senescence is an active
process programmed in development in which reactive oxygen species (ROS),
antioxidants, hormones and proteinases have key roles. Nodules have high levels of
the redox buffers, ascorbate and glutathione, which are important in the nodulation
process and in senescence. These metabolites decline with N-fixation as the nodule
ages but the resultant decrease in redox buffering capacity does not necessarily
Soil factors and Fe content in wild herbaceous plants - Pastor Piñeiro, Jesús; Hernández, A. J.; Estalrich, E.; Oliver, Salvador
We study the average and range Fe contents in wild plants from pastures located in West and Central Spain, and how
its Fe content is affected by some environmental factors, specially the soil variables: lithology, clay, pH, organic
matter, free CaCOs, exchangeable Ca, Mg, K and Al, available P, total N, water, climate, rainfall, temperature
range and light intensity.
Nine species were studied, four legumes: Trifolium subterraneum L. brachycalycinum Katzn. et Morley, Trifolium
subterraneum L. subterraneum, Trifolium cemuum and Ornithopus compressus; and five grasses: Agrostis
castellana, Bromus hordaceus, Cynodon dactylon, Dactylis glomerata and Holcus lanatus.
In Western Spain, the Fe mean content in T. subterraneum is 171.1...
Factorial analysis of the effect of sugarbeet vinasses on agroecological indices in horticultural field - Díez-Rojo, M. A.; López-Pérez, J. A.; González-López, M. R.; Piedra Buena, A.; Bello, A.; Almendros, G.
The impact on soil of applying sugarbeet vinasses (V) was analyzed through a field experiment in horticulture
greenhouse, arranged in a 23 factorial design. Two levels of three independent variables —application of V, use of
polyethylene cover (PC) on the soil, and soil depth (D)— on various dependent variables were studied. Vinasses favoured
crop yield and reduced the number of Meloidogyne incognita juveniles in soil. The concentrations of N, P and K increased
with the interaction VõD, with PC also increasing N concentration. The amounts of humic acids and humin decreased with
D; fulvic acid concentration increased with V, but decreased with the interaction VõPC....