Digital.CSIC
(14.269 recursos)
Repositorio institucional del Consejo Superior de Investigaciones Científicas.
Digital.CSIC es un depósito de documentos digitales, cuyo objetivo es organizar, archivar, preservar y difundir en modo de acceso abierto la producción intelectual resultante de la actividad investigadora del CSIC.
(IRN) Artículos post-print
Mostrando recursos 1 - 20 de 24
1.
New applications of light and electron microscopic techniques for the study of microbiological inclusion in amber - Ascaso, Carmen; Wierzchos, Jacek; Corral, J. Carmelo; López, Rafael; Alonso, Jesús
Amber is a superb medium for the fossilization of delicate organisms. Besides light microscopy techniques for the study
of insects in amber, scanning electron microscopy (SEM) in backscattered electron mode (SEM-BSE), low temperature SEM (LTSEM)
and also confocal laser scanning microscopy (CLSM) were used to examine microcenosis and particulate plant remains (microdebris).
We applied these techniques to such inclusions in amber A´ lava, northern Spain (Allaian: Early Cretaceous). Confocal microscopy
provides a 3D image of partial microcenosis showing bifurcate fungal hyphae. The huge potential of SEM-BSE yields high resolution
images, in which the relationship between protozoa and fungal hyphae may be observed and the characterization...
2.
Empleo de cubiertas de trébol subterráneo en olivar y viñedo frente al laboreo y no laboreo en estos agrosistemas - Pastor Piñeiro, Jesús; Hernández, A. J.
Las cubiertas de Trifolium subterraneum L.)una mezcla de los cultivares Nungarín, Daliak y Esperance),
en suelos de un olivar y un viñedo de secano, se han adaptado muy bien a los mismos.
Esta adaptación es evaluada especialmente por el porcentaje de recubrimiento que han logrado
tanto en otoño como en primavera durante cinco años consecutivos. La comparación de resultados
en relación con el laboreo y no-laboreo (uso de herbicidas) muestra cómo los tréboles subterráneos
mejoran la actividad biológica de los suelos.
3.
A salt stress-responsive cytokinin receptor homologue isolated from Medicago sativa nodules - Coba de la Peña, Teodoro; Cárcamo, Claudia B.; Almonacid, Luis; Zaballos, Angel; Lucas, M. Mercedes; Balomenos, Dimitrios; Pueyo, José J.
A new cytokinin receptor homologue, MsHK1,
was isolated from Medicago sativa root nodules. MsHK1
expression was induced in alfalfa seedlings by exogenous
application of the cytokinin trans-zeatin. Transcript accumulation
was detected in diVerent plant organs. MsHK1
expression was induced by salt stress in alfalfa roots, leaves
and nodules, and transcript accumulation in the vascular
bundles pointed to a putative role in osmosensing for
MsHK1 and/or other close cytokinin receptor homologues.
Expression in the meristem and the invasion zone of the
nodule suggest a role for cytokinin receptors in cytokinin
sensing during nodule cell division and diferentation
4.
Colonisatoin of Pinus halepensis roots by Pseudomonas fluorescens and interaction with the ectomycorrhizal fungus Suillus granulatus - Rincón Herranz, A.; Ruiz-Díez, Beatriz; García-Fraile, Sonia; Lucas García, José Antonio; Fernández-Pascual, Mercedes; Pueyo, José J.; Felipe, Mª Rosario de
Colonisation of Pinus halepensis roots by GFP-tagged Pseudomonas fluorescens Aur6 was monitored by epifluorescence microscopy
and dilution plating. Aur6-GFP was able to colonise and proliferate on P. halepensis roots. Co-inoculation with the ectomycorrhizal
fungus Suillus granulatus did not affect the bacterial colonisation pattern whereas it had an effect on bacterial density.
Bacterial counts increased during the first 20 days of seedling growth, irrespective of seedlings being mycorrhizal or not. After
40 days, bacterial density significantly decreased and bacteria concentrated on the upper two-thirds of the pine root. The presence
of S. granulatus significantly stimulated survival of bacteria in the root elongation zone where fungal colonisation...
5.
Shhot water status and ABA responses of transgenic hybrid larch Larix kaempferi x L. decidua to ectomyccorrhizal fungi and osmotic stress - Rincón, Ana; Priha, Outi; Lelu Walter, Marie Anne; Bonnet, Magda; Sotta, Bruno; Le Tacon, François
It has been postulated that osmotic effects on
plant tissue are mediated by abscisic acid (ABA). Hybrid larch
(Larix kaempferi (Lambert) Carr. × L. decidua Mill.) plantlets,
transformed with theABA-inducible wheatEmpromoter associated
with the Gus reporter gene, were axenically inoculated
with two ectomycorrhizal fungi: Cenococcum geophilum Fr.,
considered tolerant to water stress, and Laccaria bicolor (Marie)
Orton, considered less tolerant to drought. The mycorrhizal
and non-mycorrhizal transgenic plantlets were subjected
to osmotic stress by adding polyethylene glycol (PEG) to the
culture medium. In the presence of PEG, L. bicolor and C. geophilum
reduced shoot water potential and turgor potential, but
increased host osmotic potential. Treatment of plantlets with
PEG induced a significant increase...
6.
Comparative effects of auxin transport inhibitors on rhizogenesis and mycorrhizal establishment of spruce seedlings inoculated with Laccaria bicolor - Rincón, Ana; Priha, Outi; Sotta, Bruno; Bonnet, Magda; Le Tacon, François
We compared the effects of two auxin transport
inhibitors (2,3,5-triiodobenzoic acid (TIBA) and 1-N-naphthylphthalamic
acid (NPA)) on rhizogenesis and mycorrhizal
establishment of Picea abies L. (Karst.) seedlings inoculated
with Laccaria bicolor S238N (Maire) Orton. Inoculation of
seedlings with L. bicolor under in vitro conditions strongly increased
host root and shoot growth. Although TIBA had no effect
on taproot growth, NPA decreased taproot growth and
deformed the root apex into a globular shape in both non-inoculated
seedlings and seedlings inoculated with L. bicolor. Inoculation
with L. bicolor strongly increased lateral rhizogenesis
of the seedlings, and application of 100 μM indole-3-acetic
acid (IAA) partially reproduced this effect. Although TIBA
completely inhibited the stimulatory effect of...
7.
Influence of the fertilisation method in controlled ectomycorrhizal inoculation of two Mediterranean pines - Rincón, Ana; Parladé, J.; Pera, J.
The influence of the fertilisation method: soluble (SF) vs. slow-release fertiliser (SRF) and of inoculation with Laccaria laccata (Scop.)
Fr., Pisolithus tinctorius (Pers.) Coker & Couch and Melanogaster ambiguus (Vittad.) Tul & C. Tul. on ectomycorrhizal colonization and growth of
Pinus pinea L. and Pinus pinaster Ait. was evaluated. For both pines, mycorrhization with L. laccata was not affected by the fertilisation method.
Percentages of ectomycorrhizas (ECM) formed by P. tinctorius were dependent on the fertilisation method, the inoculum type (vegetative or spores)
and the pine species involved. ECM formed by M. ambiguus were increased with fertilisation in both pines. Inoculation significantly improved P....
8.
Effects of ectomycorrhizal inoculation and the type of substrate on mycorrhization, growth and nutrition of containerised Pinus pinea L. seedlings produced in a commercial nursery - Rincón, Ana; Parladé, J.; Pera, J.
Spore inocula of Melanogaster ambiguus (Vittad.) Tul. & C. Tul., Pisolithus tinctorius (Pers.) Coker & Couch, Rhizopogon luteolus
Fr., Rhizopogon roseolus (Corda) Th. M. Fr. et Scleroderma verrucosum (Bull.) Pers., and two substrates (a mixture of peat-vermiculite and a
mixture of peat-composted pine bark) were evaluated for producing mycorrhizal Pinus pinea L. seedlings in a commercial nursery in NE Spain.
Inocula of all fungi were effective to obtain containerised mycorrhizal P. pinea seedlings although seedling mycorrhization rates were reduced
in the peat/pine-bark mixture. Neither inoculation nor the type of substrate modified the first-year growth of seedlings. Rhizopogon spp.
increased the levels of N and P...
10.
Activation of Bean (Phaseolus vulgaris) α-Amylase Inhibitor Requires Proteolytic Processing of the Proprotein - Pueyo, José J.; Hunt, Dale C.; Chrispeels, Maarten J.
Seeds of the common bean (Pbaseolus vulgaris) contain a plant
defense protein that inhibits the a-amylases of mammals and
insects. This a-amylase inhibitor (aAl) is synthesized as a proprotein
on the endoplasmic reticulum and is proteolytically processed after
arrival in the protein storage vacuoles to polypeptides of relative
molecular weight (M,) 15,000 to 18,000. We report two types of
evidence that proteolytic processing is linked to activation of the
inhibitory activity. First, by surveying seed extracts of wild accessions
of P. vulgaris and other species in the genus Pbaseolus, we
found that antibodies to uAl recognize large (M. 30,000-35,000)
polypeptides as well as typical aAl processing products (M, 15,000-
18,000). aAl...
12.
Fluvial geomorphology and hydrology in the dispersal and fate of pyrite mud particles released by the Aznalcóllar mine tailings spill - Gallart Gallego, Francesc; Benito, Gerardo; Martín Vide, J. P.; Benito, A.; Prió, J. M.; Regüés, D.
The Agrio-Guadiamar River reach affected by the Aznalcóllar mine tailings spill can be divided into three main sectors taking account of geomorphic characteristics and human-induced changes. Along the first 15 km, the valley presents the characteristics of a middle fluvial reach of moderate sinuosity and gradient; the floodplain declines gently towards the main channel and is built up mainly by lateral accretion deposits, which are mined by open pits. The second sector is located between 15 and 30 km downstream of the mine, and is characterised by higher channel sinuosity and a lower gradient; the floodplain is built on overbank...
13.
Hydrological and geomorphological criteria to evaluate the dispersion risk of waste sludge generated by the Aznalcollar mine spill (SW Spain) - Benito, Gerardo; Gallart Gallego, Francesc; Martín-Vide, J. P.; Regüés, D.; Bladé, E.
Following the Aznalcóllar pyrite mine disaster (Seville, Spain) which caused the spilling of some 4.5 hm3 of acid water, the floodplains of the rivers Agrio and Guadiamar were rapidly cleaned of waste sludge. However, despite the efficiency of cleaning activities, there is still evidence of a fine superficial layer of sludge and some soil contamination, with the consequent risk of remobilisation of the pollutants by water erosion. There is much concern that these contaminated sediments may affect the precious ecosystems of the Doñana National Park and the Guadalquivir marshlands. This report describes the evaluation of the risk of mobilisation of the...
17.
Export of nitrogen from catchments: A worldwide analysis - Álvarez Cobelas, Miguel; Angeler, D. G.; Sánchez Carrillo, S.
9 pages, 4 figures.-- PMID: 18374463 [PubMed].-- Printed version published on Nov. 2008.-- Supplementary data available: Tables: Data sources of nitrogen export by world catchments and catchment characterization.
18.
Estimación de caudales de crecida en pequeñas cuencas de montaña: revisión metodológica y aplicación a la cuenca de Montardit (Pirineos Centrales, España). - Rico, M.; Benito, Gerardo
[ES] En este estudio se analiza la magnitud y frecuencia de las crecidas en una pequeña cuenca (15,2 Km2)
de los Pirineos Centrales españoles (cuenca de Montardit) mediante el uso combinado de diferentes métodos basados
en evidencias geológicas, históricas y datos instrumentales. De acuerdo con los datos históricos las mayores
crecidas registradas en la cuenca durante el siglo XX se produjeron en 1907, 1924 y 1944. Los caudales estimados
mediante técnicas de reconstrucción de paleocrecidas (7 a 46 m3 s- 1) y métodos hidrometeorológicos (8 a 46 m3 s- 1)
son similares para eventos considerados de elevada frecuencia y baja magnitud. Por el contrario, las...
19.
Riesgo de inundaciones: Tendencias históricas y perspectivas de acuerdo con el Cambio Climático - Benito, Gerardo
[ES] En este trabajo se analiza la relación clima-crecidas a partir del estudio de series instrumentales, paleocrecidas
y de crecidas documentales. Los registros de paleocrecidas y de crecidas documentales muestran una mayor frecuencia
de crecidas durante los estadios iniciales y finales de periodos fríos como la Pequeña Edad del Hielo (1550-
1850 AD) o la pulsación de carácter frío y húmedo ocurrida hace 2650 años. En el periodo instrumental (1910 hasta
la actualidad), los ríos atlánticos han experimentado una disminución de la frecuencia de las crecidas ordinarias, aunque
la magnitud de las crecidas catastróficas se han mantenido e incluso aumentado a pesar del efecto laminador...
20.
Microstructural Characterization of Cyanobacterial Mats from the McMurdo Ice Shelf, Antarctica - Ríos, Asunción de los; Ascaso, Carmen; Wierzchos, Jacek; Fernández-Valiente, Eduardo; Quesada, Antonio
The three-dimensional structures of two types of cyanobacterium-dominated microbial mats from meltwater ponds on the McMurdo Ice Shelf were as determined by using a broad suite of complementary techniques, including optical and fluorescence microscopy, confocal scanning laser microscopy, scanning electron microscopy with back-scattered electron-imaging mode, low-temperature scanning electron microscopy, and microanalyitical X-ray energy dispersive spectroscopy. By using a combination of the different in situ microscopic techniques, the Antarctic microbial mats were found to be structures with vertical stratification of groups of cyanobacteria and mineral sediments, high contents of extracellular polymeric substances, and large void spaces occupied by water. In cyanobacterium-rich...
1.
