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Digital.CSIC (162.855 recursos)

Repositorio institucional del Consejo Superior de Investigaciones Científicas. Digital.CSIC es un depósito de documentos digitales, cuyo objetivo es organizar, archivar, preservar y difundir en modo de acceso abierto la producción intelectual resultante de la actividad investigadora del CSIC.

(IAS) Artículos post-print

Mostrando recursos 1 - 20 de 72

  1. A metabolomic study in oats (Avena sativa) highlights a drought tolerance mechanism based upon salicylate signalling pathways and the modulation of carbon, antioxidant and photo-oxidative metabolism

    Sánchez-Martín, Javier; Heald, Jim; Kingston-Smith, Alison; Winters, Ana; Rubiales, Diego; Sanz, M. Luz; Mur, Luis A. J.; Prats, Elena
    Although a wealth of information is available on the induction of one or several drought-related responses in different species, little is known of how their timing, modulation and crucially integration influence drought tolerance. Based upon metabolomic changes in oat (Avena sativa L.), we have defined key processes involved in drought tolerance. During a time course of increasing water deficit, metabolites from leaf samples were profiled using direct infusion–electrospray mass spectroscopy (DI-ESI-MS) and high-performance liquid chromatography (HPLC) ESI-MS/MS and analysed using principal component analysis (PCA) and discriminant function analysis (DFA). The involvement of metabolite pathways was confirmed through targeted assays of key...

  2. Relationship of runoff, erosion and sediment yield to weather types in the Iberian Peninsula

    Nadal-Romero, Estela; Lasanta Martínez, Teodoro; Gómez Calero, José Alfonso

  3. No-tillage permanent bed planting and controlled traffic in a maize-cotton irrigated system under Mediterranean conditions: Effects on soil compaction, crop performance and carbon sequestration

    Cid, Patricio; Carmona Moreno, I.; Murillo Carpio, José Manuel; Gómez Macpherson, H.
    11 páginas.-- 8 figuras.-- 2 tablas.-- 66 referencias

  4. The shutdown of celiac disease-related gliadin epitopes in bread wheat by RNAi provides flours with increased stability and better tolerance to over-mixing

    Gil Humanes, Javier; Pistón, Fernando; Barro Losada, Francisco; Rosell, Cristina M.
    Celiac disease is a food-sensitive enteropathy triggered by the ingestion of wheat gluten proteins and related proteins from barley, rye, and some varieties of oat. There are no interventional therapies and the only solution is a lifelong gluten-free diet. The down-regulation of gliadins by RNAi provides wheat lines with all the gliadin fractions strongly down-regulated (low-gliadin). The technological properties of doughs prepared from the low-gliadin lines indicated a general weakening effect, although some of the lines displayed similar properties to that of the wild-type lines. In contrast, the stability was increased significantly in some of the transgenic lines, indicating better...

  5. The impact of conservation agriculture on smallholder agricultural yields: A scoping review of the evidence

    Brouder, Sylvie M.; Gómez Macpherson, H.
    Widespread implementation of conservation agriculture (CA) in North and South America and Australia suggests significant farmer profitability achieved through some combination of sustained or increased agronomic productivity and reduced input costs. Many believe similar agronomic benefits can accrue to smallholder farmers in sub-Saharan Africa (SSA) and South Asia (SA) for a broad array of crops and farming systems despite marked differences in biophysical and socio-economic environments across these regions. Our objectives were to characterize (1) the quality of existing research including an assessment of the relevance of previously published reviews and surveys to SSA and SA, and (2) the empirical...

  6. The hidden habit of the entomopathogenic fungus Beauveria bassiana: First demonstration of vertical plant transmission

    Quesada-Moraga, E.; López-Díaz, Cristina; Landa, Blanca B.
    Beauveria bassiana strain 04/01-Tip, obtained from a larva of the opium poppy stem gall wasp Iraella luteipes (Hymenoptera; Cynipidae), endophytically colonizes opium poppy (Papaver somniferum L.) plants and protects them against this pest. The goal of this study was to monitor the dynamics of endophytic colonization of opium poppy by B. bassiana after the fungus was applied to the seed and to ascertain whether the fungus is transmitted vertically via seeds. Using a species-specific nested PCR protocol and DNA extracted from surface-sterilised leaf pieces or seeds of B. bassiana-inoculated opium poppy plants, the fungus was detected within the plant beginning...

  7. Striga hermonthica MAX2 restores branching but not the Very Low Fluence Response in the Arabidopsis thaliana max2 mutant

    Liu, Qing; Fernández-Aparicio, Mónica; Bouwmeester, Harro J.
    Summary: Seed germination of Striga spp. (witchweeds), one of the world's most destructive parasitic weeds, cannot be induced by light but is specifically induced by strigolactones. It is not known whether Striga uses the same components for strigolactone signaling as host plants, whether it has endogenous strigolactone biosynthesis and whether there is post-germination strigolactone signaling in Striga. Strigolactones could not be detected in in vitro grown Striga, while for host-grown Striga, the strigolactone profile is dominated by a subset of the strigolactones present in the host. Branching of in vitro grown Striga is affected by strigolactone biosynthesis inhibitors. ShMAX2, the...

  8. Reduced-gliadin wheat bread: An alternative to the gluten-free diet for consumers suffering gluten-related pathologies

    Gil Humanes, Javier; Pistón, Fernando; Altamirano Fortoul, Rossana; Real, Ana; Comino, Isabel; Sousa Martín, Carolina; Rosell, Cristina M.; Barro Losada, Francisco
    Wheat flour cannot be tolerated by those who suffer allergies to gluten. Human pathologies associated with grain proteins have increased worldwide in recent years, and the only effective treatment available is a lifelong gluten-free diet, which is complicated to follow and detrimental to gut health. This manuscript describes the development of wheat bread potentially suitable for celiac patients and other gluten-intolerant individuals. We have made bread using wheat flour with very low content of the specific gluten proteins (near gliadin-free) that are the causal agents for pathologies such as celiac disease. Loaves were compared with normal wheat breads and rice...

  9. Phenolic content variability and its chromosome location in tritordeum

    Navas-López, José F.; Ostos-Garrido, Francisco J.; Castillo, Almudena; Martín, Antonio; Giménez, María J.; Pistón, Fernando
    For humans, wheat is the most important source of calories, but it is also a source of antioxidant compounds that are involved in the prevention of chronic disease. Among the antioxidant compounds, phenolic acids have great potential to improve human health. In this paper we evaluate the effect of environmental and genetic factors on the phenolics content in the grain of a collection of tritordeums with different cytoplasm and chromosome substitutions. To this purpose, tritordeum flour was used for extraction of the free, conjugates and bound phenolic compounds. These phenolic compounds were identified and quantified by RP-HPLC and the results...

  10. La Antracnosis del olivo y su efecto en la calidad del aceite

    Moral Moral, Juan; Xavier, José C.; Roca, Luis F.; Moreda, Wenceslao; Trapero, Antonio
    [ES] El olivo (Olea europaea subsp. europaea var. europaea) es uno de los primeros cultivos leñosos domesticados. Actualmente el olivo se encuentra ampliamente distribuido por todas las regiones de clima mediterráneo. La Antracnosis, causada por las especies complejas Colletotrichum acutatum y C. gloeosporioides, es la enfermedad del olivo que más afecta a la calidad del aceite. Aun así, el efecto de la Antracnosis en la calidad del aceite es ampliamente desconocido. Por lo que creemos esencial que se afronten estudios encaminados a dilucidar el efecto de las especies del patógeno, el cultivar de olivo y el tipo (latente o visible)...

  11. Morphological and molecular characterisation of some Hemicriconemoides species (Nematoda: Criconematidae) together with a phylogeny of the genus

    Berg, Esther van den; Tiedt, Louwrens R.; Inserra, Renato N.; Stanley, Jason D.; Vovlas, Nicola; Palomares Rius, Juan E.; Castillo, Pablo; Subbotin, Sergei A.
    Sheathoid nematodes of the genus Hemicriconemoides are migratory root-ectoparasites of many plants including various agricultural crops and fruit trees. They are generally found inhabiting warm areas of the world and presently consist of 52 valid species. In this study we provide morphological and molecular characterisation of 12 species of this genus viz.: H. alexis, H. brachyurus, H. californianus, H. chitwoodi, H. macrodorus, H. minutus, H. ortonwilliamsi, H. promissus, H. silvaticus, H. strictathecatus, H. wessoni and Hemicriconemoides sp. originating from China, Greece, Japan, Myanmar, Spain, South Africa and the USA. Morphological descriptions, measurements, light and scanning electron microscopic observations and drawings...

  12. Molecular evolution of candidate genes for crop-related traits in sunflower (Helianthus annuus L.)

    Mandel, Jennifer R.; McAssey, Edward V.; Nambeesan, Savithri; García-Navarro, Elena; Burke, John M.
    Evolutionary analyses aimed at detecting the molecular signature of selection during crop domestication and/or improvement can be used to identify genes or genomic regions of likely agronomic importance. Here, we describe the DNA sequence-based characterization of a pool of candidate genes for crop-related traits in sunflower. These genes, which were identified based on homology to genes of known effect in other study systems, were initially sequenced from a panel of improved lines. All genes that exhibited a paucity of sequence diversity, consistent with the possible effects of selection during the evolution of cultivated sunflower, were then sequenced from a panel...

  13. MC64-ClustalWP2: A highly-parallel hybrid strategy to align multiple sequences in many-core architectures

    Díaz, David; Esteban, Francisco J.; Hernández Molina, Pilar; Caballero, Juan Antonio; Guevara, Antonio; Dorado, Gabriel; Gálvez, Sergio
    We have developed the MC64-ClustalWP2 as a new implementation of the Clustal W algorithm, integrating a novel parallelization strategy and significantly increasing the performance when aligning long sequences in architectures with many cores. It must be stressed that in such a process, the detailed analysis of both the software and hardware features and peculiarities is of paramount importance to reveal key points to exploit and optimize the full potential of parallelism in many-core CPU systems. The new parallelization approach has focused into the most time-consuming stages of this algorithm. In particular, the so-called progressive alignment has drastically improved the performance,...

  14. Mapas de calendario de cultivo y variables fenológicas mediante el análisis de imágenes MODIS y ASTER basado en objetos

    Castro, Ana Isabel de; Plant, Richard E.; Six, Johan; Peña Barragán, José Manuel
    [EN] A method to generate crop calendar and phenology-related maps at a parcel level of four major irrigated crops (rice, maize, sunflower and tomato) is shown. The method combines images from the ASTER and MODIS sensors in an object-based image analysis framework, as well as testing of three different fitting curves by using the TIMESAT software. Averaged estimation of calendar dates were 85%, from 92% in the estimation of emergence and harvest dates in rice to 69% in the case of harvest date in tomato.

  15. Long-term implications of water erosion in olive-growing areas in southern Spain arising from a model-based integrated assessment at hillside scale

    Ibáñez, Javier; Martínez Valderrama, Jaime; Taguas, E. V.; Gómez Calero, José Alfonso
    In this paper, a model-based integrated assessment of the long-term consequences of water erosion in four olive groves with different conditions in Andalusia (southern Spain) is carried out. The assessment is based on a system dynamics model built at hillside scale. The modelling approach tackles common difficulties arising from the relative scarcity of data and the uncertainty of the long term. On the one hand, model results have allowed characterising the nonlinear dynamics of water erosion. On the other, they have showed that positive gross margins may definitively vanish after around 100years in some olive-growing areas. In spite of this,...

  16. Hydrological and erosive response of a small catchment under olive cultivation in a vertic soil during a five-year period: Implications for sustainability

    Gómez Calero, José Alfonso; Vanwalleghem, Tom; Hoces, Ana de; Taguas, E. V.
    This study presented monitoring results during five hydrological years representative of the typical climate conditions in the region, in a commercial olive farm on a vertic soil with a soil management based on limited tillage and growing of natural weed vegetation along the traffic lanes. A significant sediment delivery to streams equal to 16.1 t ha-1 year-1 in average was observed, whereas especially high values during years with rainfall depth above the average annual rainfall, up to 52 t ha-1 year-1 were recorded. Our observations within the catchment indicate that rill and gully erosion were major erosion processes which require...

  17. Global and time-resolved monitoring of crop photosynthesis with chlorophyll fluorescence

    Guanter, Luis; Zarco-Tejada, Pablo J.; Griffis, Timothy J.
    Guanter, Luis et al.

  18. Genetic and physical mapping of the QTLAR3 controlling blight resistance in chickpea (Cicer arietinum L)

    Madrid, Eva; Seoane, Pedro; Claros, M. G.; Barro Losada, Francisco; Rubio, Josefa; Gil, Juan; Millán, Teresa
    Physical and genetic maps of chickpea a QTL related to Ascochyta blight resistance and located in LG2 (QTLAR3) have been constructed. Single-copy markers based on candidate genes located in the Ca2 pseudomolecule were for the first time obtained and found to be useful for refining the QTL position. The location of the QTLAR3 peak was linked to an ethylene insensitive 3-like gene (Ein3). The Ein3 gene explained the highest percentage of the total phenotypic variation for resistance to blight (44.3 %) with a confidence interval of 16.3 cM. This genomic region was predicted to be at the Ca2 physical position...

  19. First report of the root-knot nematode, Meloidogyne hispanica, infecting sunflower in Greece

    Tzortzakakis, E. A.; Anastasiadis, A. I.; Simoglou, K. B.; Cantalapiedra-Navarrete, C.; Palomares Rius, Juan E.; Castillo, Pablo
    Severe plant stunting, chlorosis, and extensive root galling were observed on sunflower (Helianthus annus Pioneer Hi-bred PR64LE19, Dupont) in a commercial field at Agios Athanasios, Drama Province, northeastern Greece at the end of May 2013. Disease symptoms were observed about 1.5 months after planting, and were distributed in patches that covered approximately 2% of the whole cultivated area. Examination of the soil and root samples from selected infected plants revealed the presence of abundant root-knot nematodes. Juveniles, males, and females were extracted by sieving, decanting, and root dissection for identification using morphological traits. Nematode population densities ranging from 100 to...

  20. First report of Phaeoacremonium venezuelense associated with wood decay of apricot trees in Spain

    Olmo, D.; Gramaje, David; Agustí-Brisach, Carlos; Armengol, J.
    In July 2011, a survey was conducted to evaluate the phytosanitary status of apricot trees (Prunus armeniaca L.) in an orchard in Binissalem (Mallorca Island, Spain). Fungal isolation was performed on a 40-year-old apricot trees (cv. Galta Vermella, double-grafted onto bitter almond and Japanese plum) showing a collapse of branches, chlorosis of leaves, and shoot dieback. These symptoms appeared in approximately 10% of the trees. Black spots and dark streaking of the xylem vessels were observed in cross- or longitudinal sections of the branches. Symptomatic branches were collected and wood sections (10 cm long) were cut, washed under running tap...

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