Quilles, J. C. J.; Aragon, Caio C.; Fernández-Lorente, Gloria; Guisán, José Manuel
Most of lipases are in equilibrium between a majority inactive closed form and a minority active open form in aqueous media. Perhaps, a certain stabilization of these open forms of lipases could be achieved in the presence of cosolvents or surfactants in the reaction medium. Three commercial lipases were studied (from Thermomyces lanuginosa (TLL), Candida Antarctica fraction B (CALB) and Lecitase (LEC)). Different derivatives were tested: TLL and LEC were adsorbed on an anionic exchanger and their activity strongly depends on the equilibrium between their open and closed form and CALB was adsorbed on a hydrophobic support when the open...
Malki, Moustafá; Casado, Santiago; López, María Francisca; Caillard, Renaud; Palomares, F. Javier; Vaz-Domínguez, Cristina; Cuesta, Angel; Amils, Ricardo; Fernández López, Víctor Manuel; Vélez, Marisela; Lacey, Antonio L. de; Olea, David; Martín-Gago, José A.
The biofilm formation of a strain of the extremophile bacterium Acidiphilium sp., capable of donating electrons directly to electrodes, was studied by different surface characterization techniques. We develop a method that allows the simultaneous study of bacterial biofilms by means of fluorescence microscopy and atomic force microscopy (AFM), in which transparent graphitic flakes deposited on a glass substrate are used as a support for the biofilm. The majority of the cells present on the surface were viable, and the growth of the biofilms over time showed a critical increase of the extracellular polymeric substances (EPS) as well as the formation...
Diego Poza, Luis F. de; García Labiano, Francisco; Adánez Elorza, Juan; Gayán Sanz, Pilar; Abad Secades, Alberto; Corbella, Beatriz M.; Palacios, José María
In a chemical-looping combustion (CLC) process, gas (natural gas, syngas, etc) is burnt in two reactors. In the first one, a metallic oxide that is used as oxygen source is reduced by the feeding gas to a lower oxidation state, being CO2 and steam the reaction products. In the second reactor, the reduced solid is regenerated with air to the fresh oxide, and the process can be repeated for many successive cycles. CO2 can be easily recovered from the outlet gas coming from the first reactor by simple steam condensation. Consequently, CLC is a clean process for the combustion of...
García-Ruiz, Eva; González-Pérez, David; Ruiz-Dueñas, F. J.; Martínez, Ángel T.; Alcalde Galeote, Miguel
12 páginas, 5 figuras, 2 tablas -- PAGS nros. 487-498
Martín-Luengo, María Ángeles; Yates Buxcey, Malcolm; Ramos Gómez, Milagros; Sáez Rojo, Eduardo; Martínez-Serrano, Alberto; González Gil, Lorena; Ruiz-Hitzky, Eduardo
Agricultural wastes are a source of renewable raw materials (RRM), with structures that can be tailored for the
use envisaged. Here, theyhave proved to be good replacement candidates for use as biomaterials for the growth
of osteoblasts in bone replacement therapies. Their preparation is more cost effective than that of materials
presentlyin use with the added bonus of converting a low-cost waste into a value-added product. Due to their
origin these solids are ecomaterials.
In this study, several techniques, including X-ray diffraction (XRD), chemical analysis, mercury intrusion
porosimetry(MIP), scanning electron microscopy(SEM), and bioassays, were used to compare the biocompatibilityand
cell growth of scaffolds produced from beer bagasse,...
López Antón, María Antonia; Díaz Somoano, Mercedes; García Fierro, José Luis; Martínez Tarazona, María Rosa
7 pages, 7 figures, 6 tables.-- Printed version published Aug 2007.