Recursos de colección

Digital.CSIC (158.952 recursos)

Repositorio institucional del Consejo Superior de Investigaciones Científicas. Digital.CSIC es un depósito de documentos digitales, cuyo objetivo es organizar, archivar, preservar y difundir en modo de acceso abierto la producción intelectual resultante de la actividad investigadora del CSIC.

(IBGM) Artículos post-print

Mostrando recursos 1 - 20 de 42

  1. Tungstate-targeting of BKαβ1 channels tunes ERK phosphorylation and cell proliferation in human vascular smooth muscle

    Fernández-Mariño, Ana Isabel; Cidad, Pilar; López-López, José R.; Pérez-García, M. Teresa; Fernández-Fernández, José M.
    This is an open access article distributed under the terms of the Creative Commons Attribution License.-- et al.

  2. Tungstate-targeting of BKαβ1 channels tunes ERK phosphorylation and cell proliferation in human vascular smooth muscle

    Fernández-Mariño, Ana Isabel; Cidad, Pilar; López-López, José R.; Pérez-García, M. Teresa; Fernández-Fernández, José M.
    This is an open access article distributed under the terms of the Creative Commons Attribution License.-- et al.

  3. Susceptibility to excitotoxicity in aged hippocampal cultures and neuroprotection by non-steroidal anti-inflammatory drugs: role of mitochondrial calcium

    Calvo-Rodríguez, María; Sanz-Blasco, Sara; Caballero, Erica; Villalobos, Carlos; Núñez, Lucía
    © 2014 International Society for Neurochemistry. Brain damage after insult and cognitive decline are related to excitotoxicity and strongly influenced by aging, yet mechanisms of aging-dependent susceptibility to excitotoxicity are poorly known. Several non-steroidal anti-inflammatory drugs (NSAIDs) may prevent excitotoxicity and cognitive decline in the elderly by an unknown mechanism. Interestingly, after several weeks in vitro, hippocampal neurons display important hallmarks of neuronal aging in vivo. Accordingly, rat hippocampal neurons cultured for several weeks were used to investigate mechanisms of aging-related susceptibility to excitotoxicity and neuroprotection by NSAIDs. We found that NMDA increased cytosolic Ca2+ concentration in young, mature and...

  4. Susceptibility to excitotoxicity in aged hippocampal cultures and neuroprotection by non-steroidal anti-inflammatory drugs: role of mitochondrial calcium

    Calvo-Rodríguez, María; Sanz-Blasco, Sara; Caballero, Erica; Villalobos, Carlos; Núñez, Lucía
    Brain damage after insult and cognitive decline are related to excitotoxicity and strongly influenced by aging, yet mechanisms of aging-dependent susceptibility to excitotoxicity are poorly known. Several non-steroidal anti-inflammatory drugs (NSAIDs) may prevent excitotoxicity and cognitive decline in the elderly by an unknown mechanism. Interestingly, after several weeks in vitro, hippocampal neurons display important hallmarks of neuronal aging in vivo. Accordingly, rat hippocampal neurons cultured for several weeks were used to investigate mechanisms of aging-related susceptibility to excitotoxicity and neuroprotection by NSAIDs. We found that NMDA increased cytosolic Ca2+ concentration in young, mature and aged neurons but only promoted apoptosis...

  5. Susceptibility to excitotoxicity in aged hippocampal cultures and neuroprotection by non-steroidal anti-inflammatory drugs: role of mitochondrial calcium

    Calvo-Rodríguez, María; Sanz-Blasco, Sara; Caballero, Erica; Villalobos, Carlos; Núñez, Lucía
    Brain damage after insult and cognitive decline are related to excitotoxicity and strongly influenced by aging, yet mechanisms of aging-dependent susceptibility to excitotoxicity are poorly known. Several non-steroidal anti-inflammatory drugs (NSAIDs) may prevent excitotoxicity and cognitive decline in the elderly by an unknown mechanism. Interestingly, after several weeks in vitro, hippocampal neurons display important hallmarks of neuronal aging in vivo. Accordingly, rat hippocampal neurons cultured for several weeks were used to investigate mechanisms of aging-related susceptibility to excitotoxicity and neuroprotection by NSAIDs. We found that NMDA increased cytosolic Ca2+ concentration in young, mature and aged neurons but only promoted apoptosis...

  6. Oleanolic acid: a promising neuroprotective agent for cerebral ischemia

    Caltana, Laura; Nieto, María Luisa; Brusco, Alicia
    License Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported (CC BY-NC-SA 3.0).

  7. Oleanolic acid: a promising neuroprotective agent for cerebral ischemia

    Caltana, Laura; Nieto, María Luisa; Brusco, Alicia
    License Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported (CC BY-NC-SA 3.0).

  8. Association of type and location of BRCA1 and BRCA2 mutations with risk of breast and ovarian cancer

    Rebbeck, Timothy R.; Osorio, Ana; Benitez, Javier; Tejada, María Isabel; Durán, Mercedes; Hoya, Miguel de la; Díez, Orland; Blanco, Ignacio
    CIMBA Consortium et al.

  9. Association of type and location of BRCA1 and BRCA2 mutations with risk of breast and ovarian cancer

    Rebbeck, Timothy R.; Osorio, Ana; Benitez, Javier; Tejada, María Isabel; Durán, Mercedes; Hoya, Miguel de la; Díez, Orland; Blanco, Ignacio
    CIMBA Consortium et al.

  10. Differential calcium handling by the cis and trans regions of the Golgi apparatus

    Aulestia, Francisco J.; Alonso, María Teresa; García-Sancho, Javier
    High Ca2+ content in the Golgi apparatus (Go) is essential for protein processing and sorting. In addition, the Go can shape the cytosolic Ca2+ signals by releasing or sequestering Ca2+. We generated two new aequorin-based Ca2+ probes to specifically measure Ca2+ in the cis/cis-to-medial-Go (cGo) or the trans-Go (tGo). Ca2+ homoeostasis in these compartments and in the endoplasmic reticulum (ER) has been studied and compared. Moreover, the relative size of each subcompartment was estimated from aequorin consumption. We found that the cGo accumulates Ca2+ to high concentrations (150-300 μM) through the sarco plasmic/endoplasmic reticulum Ca2+-ATPase (SERCA). The tGo, in turn, is...

  11. Differential calcium handling by the cis and trans regions of the Golgi apparatus

    Aulestia, Francisco J.; Alonso, María Teresa; García-Sancho, Javier
    High Ca2+ content in the Golgi apparatus (Go) is essential for protein processing and sorting. In addition, the Go can shape the cytosolic Ca2+ signals by releasing or sequestering Ca2+. We generated two new aequorin-based Ca2+ probes to specifically measure Ca2+ in the cis/cis-to-medial-Go (cGo) or the trans-Go (tGo). Ca2+ homoeostasis in these compartments and in the endoplasmic reticulum (ER) has been studied and compared. Moreover, the relative size of each subcompartment was estimated from aequorin consumption. We found that the cGo accumulates Ca2+ to high concentrations (150-300 μM) through the sarco plasmic/endoplasmic reticulum Ca2+-ATPase (SERCA). The tGo, in turn, is...

  12. Hepatocyte growth factor is elevated in amniotic fluid from obese women and regulates placental glucose and fatty acid metabolism

    Visiedo, Francisco; Bugatto, Fernando; Carrasco-Fernández, C.; Sáez-Benito, A.; Mateos, R. M.; Cózar-Castellano, Irene; Bartha, Jose L.; Perdomo, Germán
    [Introduction]: To evaluate the impact of the pro-inflammatory cytokine hepatocyte growth factor (HGF) on the regulation of glucose and lipid placental metabolism. [Methods]: HGF levels were quantified in amniotic fluid and placenta from control and obese women. 2-deoxy-glucose (2-DOG) uptake, glycolysis, fatty acid oxidation (FAO), fatty acid esterification, de novo fatty acid synthesis, triglyceride levels and carnitine palmitoyltransferase activities (CPT) were measured in placental explants upon addition of pathophysiological HGF levels. [Results]: In obese women, total- and -activated-HGF levels in amniotic fluid were elevated ∼24%, and placental HGF levels were ∼3-fold higher than in control women. At a similar dose...

  13. Hepatocyte growth factor is elevated in amniotic fluid from obese women and regulates placental glucose and fatty acid metabolism

    Visiedo, Francisco; Bugatto, Fernando; Carrasco-Fernández, C.; Sáez-Benito, A.; Mateos, R. M.; Cózar-Castellano, Irene; Bartha, Jose L.; Perdomo, Germán
    [Introduction]: To evaluate the impact of the pro-inflammatory cytokine hepatocyte growth factor (HGF) on the regulation of glucose and lipid placental metabolism. [Methods]: HGF levels were quantified in amniotic fluid and placenta from control and obese women. 2-deoxy-glucose (2-DOG) uptake, glycolysis, fatty acid oxidation (FAO), fatty acid esterification, de novo fatty acid synthesis, triglyceride levels and carnitine palmitoyltransferase activities (CPT) were measured in placental explants upon addition of pathophysiological HGF levels. [Results]: In obese women, total- and -activated-HGF levels in amniotic fluid were elevated ∼24%, and placental HGF levels were ∼3-fold higher than in control women. At a similar dose...

  14. Group V secreted phospholipase A2 is upregulated by IL-4 in human macrophages and mediates phagocytosis via hydrolysis of ethanolamine phospholipids

    Rubio, Julio M.; Rodríguez, Juan Pablo; Gil-de-Gómez, Luis; Guijas, Carlos; Balboa, María A.; Balsinde, Jesús
    Studies on the heterogeneity and plasticity of macrophage populations led to the identification of two major polarization states: classically activated macrophages or M1, induced by IFN-γ plus LPS, and alternatively activated macrophages, induced by IL-4. We studied the expression of multiple phospholipase A2 enzymes in human macrophages and the effect that polarization of the cells has on their levels. At least 11 phospholipase A2 genes were found at significant levels in human macrophages, as detected by quantitative PCR. None of these exhibited marked changes after treating the cells with IFN-γ plus LPS. However, macrophage treatment with IL-4 led to strong...

  15. Lazarillo-related Lipocalins confer long-term protection against type i Spinocerebellar Ataxia degeneration contributing to optimize selective autophagy

    Caño-Espinel, Manuela del; Acebes, Judith R.; Sánchez, Diego; Ganfornina, M. D.
    This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

  16. Lazarillo-related Lipocalins confer long-term protection against type i Spinocerebellar Ataxia degeneration contributing to optimize selective autophagy

    Caño-Espinel, Manuela del; Acebes, Judith R.; Sánchez, Diego; Ganfornina, M. D.
    This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

  17. Is there a relationship between brain-derived neurotrophic factor for driving neuronal auditory circuits with onset of auditory function and the changes following cochlear injury or during aging?

    Schimmang, Thomas; Durán Alonso, Beatriz; Zimmermann, Ulrike; Knipper, Marlies
    Brain-derived neurotrophic factor, BDNF, is one of the most important neurotrophic factors acting in the peripheral and central nervous system. In the auditory system its function was initially defined by using constitutive knockout mouse mutants and shown to be essential for survival of neurons and afferent innervation of hair cells in the peripheral auditory system. Further examination of BDNF null mutants also revealed a more complex requirement during re-innervation processes involving the efferent system of the cochlea. Using adult mouse mutants defective in BDNF signaling, it could be shown that a tonotopical gradient of BDNF expression within cochlear neurons is...

  18. Delphinidin activates NFAT and induces IL-2 production through SOCE in T cells

    Jara, Evelyn; Hidalgo, María A.; Hancke, Juan L.; Hidalgo, Alejandra I.; Brauchi, Sebastian; Nuñez, Luisa; Villalobos, Carlos; Burgos, Rafael A.
    Delphinidin is an anthocyanidin that possesses antioxidant and anti-inflammatory effects; however, some reports suggest that delphinidin has pro-inflammatory properties. For this reason, we assessed the effect of delphinidin on cytokine production in T cells. We demonstrated that delphinidin increased the cytosolic-free Ca2+ concentration by releasing Ca2+ from intracellular stores and increasing Ca2+ entry. The putative Ca2+ release activated Ca2+ (CRAC) channel inhibitors BTP2 and gadolinium reduced the calcium entry stimulated by the anthocyanidin. Delphinidin induced nuclear factor of activated T cells (NFAT) translocation and NFAT-Luc activity in Jurkat cells and was dependent on the CRAC channel and calcineurin pathway. Delphinidin...

  19. Delphinidin activates NFAT and induces IL-2 production through SOCE in T cells

    Jara, Evelyn; Hidalgo, María A.; Hancke, Juan L.; Hidalgo, Alejandra I.; Brauchi, Sebastian; Nuñez, Luisa; Villalobos, Carlos; Burgos, Rafael A.
    Delphinidin is an anthocyanidin that possesses antioxidant and anti-inflammatory effects; however, some reports suggest that delphinidin has pro-inflammatory properties. For this reason, we assessed the effect of delphinidin on cytokine production in T cells. We demonstrated that delphinidin increased the cytosolic-free Ca2+ concentration by releasing Ca2+ from intracellular stores and increasing Ca2+ entry. The putative Ca2+ release activated Ca2+ (CRAC) channel inhibitors BTP2 and gadolinium reduced the calcium entry stimulated by the anthocyanidin. Delphinidin induced nuclear factor of activated T cells (NFAT) translocation and NFAT-Luc activity in Jurkat cells and was dependent on the CRAC channel and calcineurin pathway. Delphinidin...

  20. Fernando de Castro and the discovery of the arterial chemoreceptors

    González, Constancio; Conde, Silvia V.; Gallego-Martin, Teresa; Olea, Elena; Gonzalez-Obeso, Elvira; Ramirez, Maria; Yubero, Sara; Agapito, Teresa; Gómez-Niño, A.; Obeso, Ana; Rigual, Ricardo; Rocher, Asunción
    This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).

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