The Open Material Transfer Agreement (OpenMTA) is designed to allow researchers to share materials on an open basis while working within the practical realities of technology transfer. Our goal it to help reduce transaction costs, support collaboration among researchers across institutional and international boundaries, promote access to materials for researchers in less privileged institutions and world regions, and provide an avenue for researchers and their institutions to be credited for materials made openly available. The OpenMTA was developed as a collaborative effort led by the BioBricks Foundation and OpenPlant Synthetic Biology Research Centre, with input from researchers, technology transfer professionals,...
Endy, Drew; Zoloth, Laurie
Given that human genome synthesis is a technology that could be used to completely redefine the core of what now joins all of humanity together as a species, we argue that discussions of making such capacities real, like today's meeting at Harvard, should not take place without open and advance consideration of whether and under what circumstances it is morally right to proceed.
Knight, Thomas; Matsudaira, Paul
Create and exploit a novel technology for information processing and manufacturing by controlling processes in living cells.
Research presentation on recent and unpublished work before the MIT Synthetic Biology Working Group on 24 September 2012 in MIT Building 56.
Shetty, Reshma; Lizarazo, Meagan; Rettberg, Randy; Knight, Thomas F., Jr.
An underlying goal of synthetic biology is to make the process of engineering biological systems easier and more reliable. In support of this goal, we developed BioBrick assembly standard 10 to enable the construction of systems from standardized genetic parts. The BioBrick standard underpins the distributed efforts by the synthetic biology research community to develop a collection of more than 6000 standard genetic parts available from the Registry of Standard Biological Parts. Here, we describe the three antibiotic assembly method for physical composition of BioBrick parts and provide step-by-step protocols. The method relies on a combination of positive and negative...
Endy, Drew; Grewal, David; Schultz, Jason; Lynch, Jennifer; Crews, Lee; Fischer, Mark
We present an updated, clean draft for additional public comment of a new legal framework supporting the future of biotechnology. The framework, named here as the BioBrick Public Agreement, is based upon the contribution of promises not to assert any property rights against users of so-contributed standard biological parts. Comments and feedback on this working draft are requested. This draft, BPA v1a, replaces and earlier draft, BPA v1.
Fischer, Mark; Crews, Lee; Lynch, Jennifer; Schultz, Jason; Grewal, David; Endy, Drew
We present a draft for public comment of a new legal framework supporting the future of biotechnology. The framework, named here as the BioBrick Public Agreement, is based upon the contribution of promises not to assert any property rights against users of so-contributed standard biological parts. Comments and feedback on this working draft are requested.
Win, Maung Nyan; Smolke, Christina
This supplement provides additional detail on the data analysis methods and the raw data for the
RNA devices presented in the published manuscript and supporting online material. The
information provided in the current supplement is organized as follows:
1. Gating methods for the raw flow cytometry data
2. Correction methods for nonspecific effects on fluorescence of chemical effectors
controlling the RNA devices
3. Selection of standard against which to report device performance
4. Relevance to conclusions published in the Science paper
5. Example calculations
6. Raw data for RNA devices
Wadey, Chuck; Deese, Isadora; Endy, Drew
3 chapter comic book reflecting the early engineering period of synthetic biology ~2005. Translations freely available by searching online.
Smolke, CD; Win, MN
Early publication of correction and clarification to referenced article in PNAS USA.
Lazowska, Ed; Endy, Drew
A 2.5 page report outlining why the United States should launch a strategic national research initiative in synthetic biology
Soergel, David; Choi, Kirindi; Thomson, Ty; Doane, Jay; George, Brian; Morgan-Linial, Ross; Brent, Roger; Endy, Drew
Research article written in 2004 describing MONOD, an early biological knowledge management system
Shetty, Reshma P
Ph.D. thesis (user submitted)
Bügl, Hans; Danner, John; Molinari, Robert; Mulligan, John; Roth, David; Wagner, Ralf; Budowle, Bruce; Scripp, Robert; Smith, Jenifer; Steele, Scott; Church, George; Endy, Drew
This is the unabridged draft of the manuscript "DNA synthesis and biological security." An abridged form of this manuscript was later published as a peer review commentary in Nature Biotechnology (doi:10.1038/nbt0607-627)
Construction of complex biological systems can require assembling many
modules together. However, existing assembly schemes are lacking in
generality, ease of use, or power to perform some desirable
operations. Currently, biological modules are most easily specified
and manipulated as DNA sequences. A general system, called
BioBricks++, for assembling and manipulating DNA modules is proposed.
BioBricks++ was inspired by the BioBricks assembly scheme but provides
for more possible module operations.
BioBricks++ uses commercially available restriction enzymes and
standard biological techniques for assembling modules. The key to the
method is in the specification of the standard DNA module. Modules are
packaged with a standard prefix and suffix DNA sequence containing
several restriction enzyme sites,...
Garfinkel, Michele; Endy, Drew; Epstein, Gerald; Friedman, Robert
Compilation of Technical Reports in Support of Sloan Foundation study on DNA synthesis and governance options
Technical Report in support of Sloan Foundation study on DNA synthesis and governance options.
Technical Report in support of the Sloan Foundation study on DNA synthesis and governance options.
Technical report in support of the Sloan Foundation study on DNA synthesis and governance options.
Technical report in support of the Sloan Foundation funded study on DNA synthesis and governance.