DIALNET OAI Articles
Dialnet (Difusión de Alertas en la Red) es una plataforma de recursos y servicios documentales, cuyo objetivo fundamental se centra en mejorar la visibilidad y el acceso a la literatura científica hispana a través de Internet.
Mostrando recursos 1 - 20 de 18.225
Influencia del grupo ecológico sobre la sensibilidad in vitro de los hongos dermatofitos a los antifúngicos - Carrillo Muñoz, Alfonso Javier; Tur Tur, Cristina; Cárdenes Perera, Carmen Delia; Rojas, Florencia; Giusiano, Gustavo
Dermatophytes can be divided into geophilic (soil), zoophilic (animals) and anthropophilic (humans) strains, depending on the source of the keratin that they use for nutritional purposes.
The in vitro susceptibility of clinical isolates of dermatophyte fungi has been studied in the 3 ecological groups with several antifungal agents for the topical management of dermatophytoses in order to determine their relationship with the ecological group.
A standardised dilution micromethod in a liquid medium was used for the determination of the in vitro antifungal activity of 9 topical antifungal drugs: amorolfine (AMR), bifonazole (BFZ), clotrimazole, econazole, ketoconazole, miconazole, oxiconazole, terbinafine (TRB) and tioconazole. The...
Heat treatment of wheat straw by immersion in hot water decreases mushroom yield in Pleurotus ostreatus - Jaramillo Mejía, Santiago; Albertó, Edgardo
The oyster mushroom, Pleurotus ostreatus, is cultivated worldwide. It is one of the most appreciated mushrooms due to its high nutritional value. Immersion of the substrate in hot water is one of the most popular and worldwide treatment used for mushroom farmers. It is cheap and easy to implement.
To compare the yields obtained during mushroom production of P. ostreatus using different pre-treatments (immersion in hot water, sterilization by steam and the use of fungicide) to determine if they influence mushroom crop.
Four different treatments of substrate (wheat straw) were carried out: (i) immersion in hot water (IHW); (ii) steam sterilization; (iii)...
Candida parapsilosis meningitis in a patient with AIDS.L Report of a case and review of the literature - Corti, Marcelo; Solari, Rubén; De Carolis, Luis; Cangelosi, Diana; Arechavala, Alicia; Negroni, Ricardo
Candida parapsilosis is an important species in the genus Candida that plays a significant role in hospitalized patients with nosocomial infections. In patients with HIV infection or AIDS, central nervous system involvement by Candida species is exceptional.
Here we report a case of an acute meningoencephalitis due to C. parapsilosis in an adult patient with AIDS. We describe the clinical manifestations, the diagnosis methods, antifungal therapy and outcome.
C. parapsilosis is uncommonly reported as a cause of meningitis in AIDS patients. A higher index of suspicion and culture is necessary to confirm the diagnosis of candidal meningoencephalitis.
Cutaneous fusariosis by a species of the Fusarium dimerum species complex in a patient with acuete mieloblastic leukemia - Collado Amores, María Carmen; Medina, Laura; Zorraquino, Alfredo; Baeza, Teresa; Ferrer, Consuelo; Plazas, Joaquín; Colom, Francisca
Fusariosis is an emergent opportunistic hyalohyphomycosis produced by fungi belonging to the genus Fusarium. These molds are capable of producing life-threatening diseases in immunocompromised hosts, especially in those suffering from leukemia. It has also been described in immunocompetent patients, where it usually causes non-invasive localized lesions. Fusariosis in immunocompromised individuals has a high morbidity and mortality mainly because of the low sensitivity of these fungi to the antifungal drugs available.
We describe here the case of a patient with acute mieloblastic leukemia who developed fusariosis by a species of the Fusarium dimerum species complex. The early diagnosis was made on...
Subcutaneous phaeophyphomycosis due to Curvularia lunata in a renal transplant patient - Vásquez Mercado, Elsa; Lammoglia, Lorena; Arenas, Roberto
Phaeohyphomycosis is defined as an infection caused by melanized fungi. It predominates in tropical climate and is currently classified as superficial, allergic, central nervous system or lung infections, and disseminated. Curvularia is one of the many genres which can cause this disease. Phialophora, Alternaria and Exophiala are more commonly isolated from subcutaneous lesions.
A 25-year-old male, renal transplant recipient presents with an ulcer on his left leg. Subcutaneous phaeohyphomycosis due to Curvularia lunata was diagnosed based on mycological examination and histopathological findings. He was successfully treated with systemic itraconazole and surgical resection.
The incidence of phaeohyphomycosis has increased in the last...
Outbreak of fungemia caused by Candida parapsilosis in a neonatal intensive care unit: Molecular investigation through microsatellite analysis - Silva Ruiz, Luciana; Cezar Montelli, Augusto; Sugizaki, María de Fátima; Gonçalves Da Silva, Eriques; Matuura De Batista, Georgea Carla; Moreira, Débora; Rodrigues de Paula, Claudete
Opportunistic infections are an increasingly common problem in hospitals, and the yeast Candida parapsilosis has emerged as an important nosocomial pathogen, especially in neonatal intensive care units (NICUs) where it has been responsible for outbreak cases. Risk factors for C. parapsilosis infection in neonates include prematurity, very low birth weight, prolonged hospitalization, indwelling central venous catheters, hyperalimentation, intravenous fatty emulsions and broad spectrum antibiotic therapy. Molecular methods are widely used to elucidate these hospital outbreaks, establishing genetic variations among strains of yeast.
The aim of this study was to detect an outbreak of C. parapsilosis in an NICU at the �Hospital...
Dermatomycosis in lower limbs of diabetic patients followed by podiatry consultation - Parada, Helena; Veríssimo, Cristina; Brandao, Joao; Nunes, Baltazar; Boavida, José; Duarte, Rui; Peerally, Zulmira; Oliveira, Rui; Rosado, Laura; Sabino, Raquel
Diabetic patients are particularly susceptible to fungal infections due to modifications that occur in their immunological system. These modifications compromise natural defences, such as skin and nails, especially from lower limbs.
Assessing the presence of dermatomycosis in lower limbs of Portuguese diabetic patients followed on Podiatry consultation. Determination of possible predisposing factors and the most frequent fungal species associated with the cases are included in the study.
A six-month prospective study was carried out in 163 diabetic patients with signs and symptoms of dermatomycosis followed by Podiatry at the Portuguese Diabetes Association in Lisbon. Samples from the skin and/or nails of the...
Preservation of Agaricus subrufescens strains at low temperature by using cultures on sorghum grains - Mata, Gerardo; Savoie Gutiérrez, J.
One of the main problems for the preservation of genetics resources of Agaricus subrufescens is to maintain the viability of the strains because the mycelium is very sensitive to cooling and therefore it ages rapidly.
Evaluate the viability of A. subrufescens strains stored as cultures on sorghum grain (spawn) at different temperatures.
Eighteen strains of A. subrufescens and three strains of Agaricus bisporus were studied. Spawn's viability was evaluated under the following conditions: (1) control at 25 °C (C), (2) cooling to 4 °C (R) and (3) freezing in liquid nitrogen at -196 °C (LN). Samples were recovered from week 4 every...
Developing a taxonomic identification system of Phytophthora species based on microsatellites - Castillo Munuera, Johanna; Cárdenas, Martha Emiliana; Pinzón, Andrés M.; Castañeda, Adriana; Bernal, Adriana; Restrepo, Silvia
Phytophthora is the most important genus of the Oomycete plant pathogens. Nowadays, there are 117 described species in this genus, most of them being primary invaders of plant tissues. The different species are causal agents of diseases in a wide range of crops and plants in natural environments. In order to develop control strategies against Phytophthoraspecies, it is important to know the biology, ecology and evolutionary processes of these important pathogens.
The aim of this study was to propose and validate a low cost identification system for Phytophthora species based on a set of polymorphic microsatellite (SSRs) markers.
Thirty-three isolates representing Phytophthora...
Physiological and molecular characterization of Phytophthora infestans isolates from the Central Colombian Andean Region - Céspedes, María C.; Cárdenas, Martha Emiliana; Vargas, Angela M.; Rojas, Alejandro; Morales, Juan G.; Jiménez, Pedro; Bernal, Adriana; Restrepo, Silvia
Late blight, caused by Phytophthora infestans, is one of the most devastating diseases found in potato and tomato crops worldwide. In Colombia it also attacks other important crops: cape gooseberry and tree tomato. The knowledge of the pathogen population is determinant to effectively design control strategies.
To determine the physiological and molecular characteristics of a set of Colombian P. infestans isolates.
Strains isolated from Cundinamarca and Boyacá were examined for the level of resistance to mefenoxam and cymoxanil. Virulence was tested for all strains and crosses between A1 mating type, from different hosts, and the Colombian A2 mating type were tested for...
Problemas clínicos en micología médica: problema número 47 - Corti, Marcelo; Muzzio, Estela; Villafañe, María Florencia; Priarone, María Marta; Messina, Fernando; Arechavala, Alicia; Maiolo, Elena; Negroni, Ricardo; Franze, Óscar
A 66 year-old man, who had lived in Paraguay and was a rural worker, was admitted to Infectious Diseases Hospital F. J. Muñiz in Buenos Aires. He presented fever, loss of body weight, cough, mucopurulent expectoration, wide perianal ulceration, paresthesia and paresis of both legs as well as vesical and anal sphincter dysfunction. He was a heavy smoker and drinker. Thorax X-ray examination showed bilateral micronodular interstitial lesions. With a NMR of the dorsolumbar spine region a nodular lesion outside the spinal cord (which produced compression of this organ) was shown. The diagnosis of disseminated paracoccidiodomycosis was based on the...
The new mutation L321F in "Candida albicans" ERG11 gene may be associated with fluconazole resistance - Carvalho, Vagner Oliveira; Okay, Thelma S.; Melhem, Márcia S.C.; Szeszs, María Walderez; Negro, Gilda M.B. del
For many years fluconazole has been commonly used to treat Candida infections. However, the indiscriminate use of this antimycotic therapy has favored the emergence of resistant isolates. Mutations in the ERG11 gene have been described as one of the primary mechanisms of resistance in Candida species.
In this study we investigated missense mutations in ERG11 genes of Candida albicans, Candida glabrata and Candida tropicalis isolates previously evaluated by susceptibility testing to fluconazole.
Screening for these mutations was performed on 19 Candida clinical isolates (eight C. albicans, five C. glabrata and six C. tropicalis) resistant and susceptible to fluconazole. The ERG11 gene was...
Infección por "Saccharomyces cerevisiae" - Goebel, Cristine Souza; Oliveira, Flávio de Mattos; Severo, Luiz Carlos
Saccharomyces cerevisiae is an ubiquitous yeast widely used in industry and it is also a common colonizer of the human mucosae. However, the incidence of invasive infection by these fungi has significantly increased in the last decades.
To evaluate the infection by S. cerevisiae in a hospital in southern Brazil during a period of 10 years (2000-2010).
Review of medical records of patients infected by this fungus.
In this period, 6 patients were found to be infected by S. cerevisiae. The age range of the patients was from 10 years to 84. Urine, blood, ascitic fluid, peritoneal dialysis fluid, and esophageal biopsy samples...
Synergysm of voriconozale or itraconozale with other antifungal agents against species of "Fusarium" - Spader, Tatiana B.; Venturini, Tarcieli P.; Rossato, Luana; Denardi, Laura B.; Cavalheiro, Patricia B.; Botton, Sonia A.; Santurio, Janio Morais; Alves, Sydney Hartz
Infections caused by Fusarium are difficult to treat because these fungi show in vitro and in vivo resistance to practically all the antifungal agents available, which explains the high mortality rates. An attempt to overcome fungal resistance is the combination of antifungal agents, especially those with different mechanisms of action.
Evaluate the in vitro interactions of combinations of voriconazole or itraconazole with other antifungal agents against 32 isolates of Fusarium spp.: Fusarium chlamydosporum, Fusarium oxysporum, Fusarium proliferatum and Fusarium solani.
Drug interactions were assessed by a checkerboard microdilution method that also included the determination of the MIC of each drug alone according...
Brote de histoplasmosis en la provincia de Neuquén, Patagonia argentina - Zuiani, María Fernanda; Negroni, Ricardo; Finquelievich, Jorge; Canteros, Cristina Elena; Calanni, Liliana María; Pérez, Rufina Ana; Brasili, Susana; Schmidt, Norma Graciela; Iovannitti, Cristina
Background: In Argentina,there are no reports of autochthonous cases of histoplasmosis in the southern regions of the country.
Aim: To report a histoplasmosis outbreak in Zapala town, Province of Neuquén, Patagonia Argentina.
Methods: We evaluated the clinical and epidemiological characteristics of 5 patients involved in the outbreak. Environmental studies were conducted to determine the source of infection.The genetic pro?le of Histoplasma capsulatum strains isolated from the index case (IC) were compared with clinical isolates from Argentinean patients not related to the outbreak, using RAPD-PCR with primers 1281-1283.
Results: The patients were residents of Zapala, and had not visited other geographical areas before. All...