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Faculty of Technology ePrints Service (41,981 recursos)
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Mostrando recursos 1 - 20 de 10,320

1. Predicación, pedagogía y persuasión: la educación cristiana en Occidente durante la Antigüedad tardía - Wood, Jamie
Esta contribución examina la educación cristiana en la antigüedad tardía desde la perspectiva de predicadores expertos, tomando como referencia concreto los consejos que dieron a sus colegas predicadores. En ella intentamos ver de qué modo líderes eclesiásticos como Agustín instruyeron a quienes les pedían consejo sobre la manera de enseñar los fundamentos del cristianismo. Las fuentes principales de nuestro estudio son dos manuales para predicadores cristianos, escritos por obispos en respuesta a sendas peticiones de clérigos. La primera fuente es De catechizandis rudibus, de Agustín, escrito ca. 400 en la provincia de África, y la segunda De correctione rusticorum, que...

2. Yeasts associated with the New Zealand Nothofagus honeydew system - Serjeant, Kelsey; Tang, Rongying; Anfang, Nicole; Beggs, Jacqueline R.; Goddard, Matthew R.
New Zealand honeydew beech forests are the only natural ecosystem where large quantities of above-ground labile carbon are added year-round. Microbes can potentially play an important role in the flux of nutrients through food webs; because of their potential for specialisation to sugar-rich niches we tested for the presence of yeasts in the honeydew system. We found at least two abundant species (Hanseniaspora osmophila and Candida railenensis), as well as two species (Zygosaccharomyces cidri and Z rouxii) likely present at lower frequencies. Only Candida railenensis is known to be associated with Nothofagus, but the other species are associated with fruits...

3. A database of microsatellite genotypes for Saccharomyces cerevisiae - Richards, Keith D.; Goddard, Matthew R.; Gardner, Richard C.
A system for genotyping Saccharomyces cerevisiae is described based on a multiplex of ten microsatellite loci and the MAT locus. A database of genotypes has been developed for 246 yeast strains, including a large set of commercial wine yeasts, as well as 35 sequenced natural isolates currently being sequenced. The latter allow us, for the first time, to make direct comparisons of the relationship between DNA sequence data and microsatellite-based genotypes. The genotyping system provides a rapid and valuable system for strain identification as well as studying population genetics of S. cerevisiae. © Springer Science+Business Media B.V. 2009.

4. Optimized fermentation of grape juice by laboratory strains of Saccharomyces cerevisiae - Harsch, Michael J.; Lee, Soon A.; Goddard, Matthew R.; Gardner, Richard C.
Laboratory strains of yeast (Saccharomyces cerevisiae) based on S288C ferment grape juice relatively poorly. We show that slow fermentation appears to be inherent to this strain, because the original S288C isolate shows fermentation similar to current laboratory isolates. We demonstrate further that some auxotrophic mutations in the laboratory strain show reduced rates of fermentation in grape juice, with lysine auxotrophs particularly impaired compared with isogenic Lys+ strains. Supplementing lysine at a 10-fold higher concentration than recommended allowed yeast cultures to reach higher final cell densities and restored the fermentation rate of auxotrophic strains to those of the corresponding wild-type strains....

5. Sex enhances adaptation by unlinking beneficial from detrimental mutations in experimental yeast populations - Gray, Jeremy C.; Goddard, Matthew R.
Background: The maintenance of sexuality is a classic problem in evolutionary biology because it is a less efficient mode of reproduction compared with asexuality; however, many organisms are sexual. Theoretical work suggests sex facilitates natural selection, and experimental data support this. However, there are fewer experimental studies that have attempted to determine the mechanisms underlying the advantage of sex. Two main classes of hypotheses have been proposed to explain its advantage: detrimental mutation clearance and beneficial mutation accumulation. Here we attempt to experimentally differentiate between these two classes by evolving Saccharomyces cerevisiae populations that differ only in their ability to...

6. Geographic delineations of yeast communities and populations associated with vines and wines in New Zealand - Gayevskiy, Velimir; Goddard, Matthew R.
Yeasts are a diverse seemingly ubiquitous group of eukaryotic microbes, and many are naturally associated with fruits. Humans have harnessed yeasts since the dawn of civilisation to make wine, and thus it is surprising that we know little of the distribution of yeast communities naturally associated with fruits. Previous reports of yeast community diversity have been descriptive only. Here we present, we believe, the first robust test for the geographic delineation of yeast communities. Humans have relatively recently employed Saccharomyces cerevisiae as a model research organism, and have long harnessed its ancient adaption to ferment even in the presence of...

7. Gene-flow between niches facilitates local adaptation in sexual populations - Gray, Jeremy C.; Goddard, Matthew R.
In sexual populations, gene-flow between niches is predicted to have differential consequences on local adaptation contingent upon the nature of trade-offs underlying local adaptation. Sex retards local adaptation if antagonistic pleiotropy underlies trade-offs, but facilitates adaptation if mutation accumulation underlies trade-offs. We evaluate the effect of sex in heterogeneous environments by manipulating gene-flow between two niches in sexual and asexual populations using steady-state microcosm experiments with yeast. We find that only sex in the presence of gene-flow promotes simultaneous local adaptation to different niches, presumably as this exposes mutations neutrally accrued in alternate niches to selection. This finding aligns with...

8. A parasitic selfish gene that affects host promiscuity - Giraldo-Perez, Paulina; Goddard, Matthew R.
Selfish genes demonstrate transmission bias and invade sexual populations despite conferring no benefit to their hosts. While the molecular genetics and evolutionary dynamics of selfish genes are reasonably well characterized, their effects on hosts are not. Homing endonuclease genes (HEGs) are one well-studied family of selfish genes that are assumed to be benign. However, we show that carrying HEGs is costly for Saccharomyces cerevisiae, demonstrating that these genetic elements are not necessarily benign but maybe parasitic. We estimate a selective load of approximately 1-2 in 'natural' niches. The second aspect we examine is the ability of HEGs to affect hosts'...

9. Quantifying variation in the ability of yeasts to attract Drosophila melanogaster - Palanca, Loida; Gaskett, Anne C.; Gunther, Catrin S.; Newcomb, Richard D.; Goddard, Matthew R.
Yeasts that invade and colonise fruit significantly enhance the volatile chemical diversity of this ecosystem. These modified bouquets are thought to be more attractive to Drosophila flies than the fruit alone, but the variance of attraction in natural yeast populations is uncharacterised. Here we investigate how a range of yeast isolates affect the attraction of female D. melanogaster to fruit in a simple two choice assay comparing yeast to sterile fruit. Of the 43 yeast isolates examined, 33 were attractive and seven repellent to the flies. The results of isolate-versus-isolate comparisons provided the same relative rankings. Attractiveness varied significantly by...

10. Pyrosequencing reveals regional differences in fruit-associated fungal communities - Taylor, Michael W.; Tsai, Peter; Anfang, Nicole; Ross, Howard A.; Goddard, Matthew R.
We know relatively little of the distribution of microbial communities generally. Significant work has examined a range of bacterial communities, but the distribution of microbial eukaryotes is less well characterized. Humans have an ancient association with grape vines (Vitis vinifera) and have been making wine since the dawn of civilization, and fungi drive this natural process. While the molecular biology of certain fungi naturally associated with vines and wines is well characterized, complementary investigations into the ecology of fungi associated with fruiting plants is largely lacking. DNA sequencing technologies allow the direct estimation of microbial diversity from a given sample,...

11. ObStruct: A method to objectively analyse factors driving population structure using Bayesian ancestry profiles - Gayevskiy, Velimir; Klaere, Steffen; Knight, Sarah; Goddard, Matthew R.
Bayesian inference methods are extensively used to detect the presence of population structure given genetic data. The primary output of software implementing these methods are ancestry profiles of sampled individuals. While these profiles robustly partition the data into subgroups, currently there is no objective method to determine whether the fixed factor of interest (e.g. geographic origin) correlates with inferred subgroups or not, and if so, which populations are driving this correlation. We present ObStruct, a novel tool to objectively analyse the nature of structure revealed in Bayesian ancestry profiles using established statistical methods. ObStruct evaluates the extent of structural similarity...

12. Clinical indicators of occult musculoskeletal pain in aggressive dogs - Barcelos, A.-M.; Mills, D. S.; Zulch, H.
AGGRESSIVE behaviour by dogs is the most frequent behaviour problem reported to veterinary behaviour clinics (Bamberger and Houpt 2006, Fatjo and others 2007). The behaviour is a serious problem for the victims (Weiss and others 1998) and for the animals themselves, since it often reflects a negative underlying emotional state, especially if associated with pain (Bamberger and Houpt 2006, Hsu and Sun 2010). Pain may lower the dog’s general threshold for aggressive behaviour, while also encouraging specific aggressive displays as part of a protective/defensive response (Rutherford 2002, Muir and others 2004, Landsberg and others 2013). Beaver (1983) has suggested that more than a quarter (28.2 per cent) of dogs exhibiting aggression...

13. How do women under the care of eating disorder services experience sibling relationships : a phenomenological perspective - Smith, Jennifer Ann
Eating disorders are increasing in our society and prior research has considered the role of families, carers, partners and children in the development of these difficulties. Siblings, however, have been largely overlooked. The role of sibling relationships is not well understood, despite siblings being a long term, significant feature of many individuals with eating disorders’ lives. This study aims to investigate the experiences of women with eating disorders and their sibling relationships. Interpretative Phenomenological Analysis (IPA) was used to investigate the lived experiences of three women with diagnosed eating disorders. The women were interviewed, using a semi structured interview schedule designed for the study, and transcripts were analysed closely, following...

14. Producing for Web 2.0: a student guide - Whittaker, Jason
Producing for the Web sets out the practical toolkit that a web producer will require to create their site, from web and image editors to information processing and programme applications.

15. Producing for Web 2.0: a student guide - Whittaker, Jason
Producing for the Web sets out the practical toolkit that a web producer will require to create their site, from web and image editors to information processing and programme applications.

16. Tracking of partially unknown trajectories for permanent magnet synchronous motors - Gentili, Luca; Paoli, Andrea; Bonivento, Claudio
In this paper, an adaptive internal model-based control architecture is designed to deal with an exogenous trajectory tracking problem for a Permanent Magnet Synchronous Motor (PMSM). More in detail, we show how to design a controller able to guarantee the asymptotic tracking of partially unknown exogenous trajectories belonging to a given family, embedding in the regulator the internal model of this family; the control algorithm is able to attain the asymptotic tracking without the knowledge of reference derivatives and exploiting only instantaneous error feedbacks. The theoretical machinery exploited is the nonlinear regulation theory, specialized for the energy-based port-Hamiltonian formalism. The...

17. Professional values in nursing - Baillie, Lesley; Black, Sharon

18. El papel del Ingeniero Industrial en la formulación de políticas públicas - Vilalta-perdomo, Eliseo Luis
- La toma de decisiones: Hay un rol para la ciencia? - Decisiones en el largo plazo: sustentabilidad - Retos de la sustentabilidad - Distintas aproximaciones - El rol del ingeniero industrial

19. A review on food safety and food hygiene studies in Ghana - Ababio, P. F.; Lovatt, P.
Food safety and hygiene in Ghana was studied using desk top literature review. Food research was highly concentrated in the capital city of the country and most research focus were on commercial food operations specifically street foods and microbiological safety with limited information from institutional catering and other forms of food hazards. The media currently serves as the main source for reporting of food borne diseases. Food establishments and other sources contributing to food borne diseases included restaurants, food joints, food vendors, schools and individual homes. Limited use of prerequisites measures and food safety management systems was identified. Recommendations on...

20. What is important in theorizing tolerance today? - Brown, Wendy; Dobbernack, Jan; Modood, Tariq; Newey, Glen; March, Andrew F.; Tonder, Lars; Forst, Rainer
if one wants to grasp tolerance politically, that is, as a problem of power and as organizing relations among citizens, subjects, peoples or states, then it must be understood, inter alia, as being enacted through contingent, historically specific discourses - linguistically organized norms operating as common sense. [...]any political discourse of tolerance - from that developed for handling Protestant sectarianism in seventeenth-century England to that used by the G.W. Bush Administration in the aftermath of 9/11 to distinguish the West from the rest, to that used by the Israeli state for describing (only) its policies toward homosexuals - is embedded...

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