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Biblioteca Digital do IPB (53.442 recursos)

A Biblioteca Digital do IPB, tem por objectivo divulgar e permitir o livre acesso à produção científica produzida pela comunidade académica, promovendo a integração, partilha e a visibilidade da informação científica e garantindo a preservação da memória intelectual do Instituto Politécnico de Bragança.

DTDT - Artigos em Revistas

Mostrando recursos 1 - 20 de 77

  1. Overview on mentha and thymus polyphenols

    Pereira, Olívia R.; Cardoso, Susana M.
    Mentha and Thymus are important genera of the Lamiaceae family widely distributed in the entire World and commonly used in traditional medicine. Indeed, many species of the two genera have been credited with a large list of health-benefit effects, including antioxidant, anti-inflammatory, antimicrobial, analgesic, neuroprotective and anticarcinogenic. In turn, these properties have been associated to the polyphenolic composition of the plants. The present review summarizes the phenolic constituents found in Mentha and Thymus genera, as well as the main methods applied in their extraction, purification and identification. Reported species of Mentha and Thymus usually comprise derivatives of caffeic acid and distinct glycosidic forms of the flavonoids...

  2. Protective effects of phenolic constituents from Cytisus multiflorus, Lamium album L. and Thymus citriodorus on liver cells

    Pereira, Olívia R.; Macias, Rócio I.R.; Perez, María J.; Marín, Jose J.G.; Cardoso, Susana M.
    The present study investigated the antioxidant and cytoprotective effects of purified ethanolic extracts of Cytisus multiflorus, Lamium album L. and Thymus citriodorus plants. These extracts showed high antioxidant activity in DPPH and reducing power assays. Using a model of chemical stress induced by potassium dichromate (DK) in human hepatoblastoma HepG2 cells, 50 ug/mL of C. multiflorus, L. album and T. citriodorus extracts decreased the rate of reactive oxygen species (ROS) production by 35%, 26% and 20%, respectively, when exposed to 25 mu M of DK. This effect was also observed for the treatment of cells with individual polyphenolic compounds determined...

  3. Phenolic constituents of lamium album: focus on isoscutellarein derivatives

    Pereira, Olívia R.; Domingues, M.R.M.; Silva, Artur; Cardoso, Susana M.
    Lamium album L. is an edible plant which is consumed raw or cooked, in particular in the Mediterranean and surrounding areas. It is also consumed as tea infusions and as a main component of food supplements, because of its pharmacological effects. Despite being consumed by humans for centuries, the chemical composition of L album L is far from being understood. In this study, a purified ethanolic extract (PEEL) was prepared and further analyzed by high performance liquid chromatography and electrospray mass spectrometry. Overall, verbascoside accounted for approximately half of the phenolic content of the extract, but this also contained other...

  4. Identification of phenolic constituents of cytisus multiflorus

    Pereira, Olívia R.; Silva, Artur; Domingues, M.R.M.; Cardoso, Susana M.
    The phenolic composition of the ethanolic extract obtained from the flowers of the medicinal plant Cytisus multiflorus has been elucidated by high performance liquid chromatography, electrospray mass spectrometry and nuclear magnetic resonance analysis. The extract was mainly composed of flavones, including the common chrysin, orientin, luteolin-5-O-glucoside, luteolin-7-O-glucoside, apigenin and apigenin-7-O-glucoside, which appeared as minor components. The major flavone in the extract was chrysin-7-O-B-D-glucopyranoside, and it also contained moderate amounts of a dihydroxyflavone isomer of chrysin, as well as of 2''-O-pentosyl-6-C-hexosyl-luteolin, 2''-O-pentosyl-8-C-hexosyl-luteolin and 6''- O-(3-hydroxy-3-methylglutaroyl)-2''-O-pentosyl-C-hexosyl-apigenin, which are not commonly found in the Fabaceae family. Other novel phenolic compounds found in the ethanolic extract of C. multiflorus comprised the flavones 2''-O-pentosyl-6-C-hexosyl-apigenin,...

  5. Bilirubin dependent on UGT1A1 polymorphisms, hemoglobin, fasting time and body mass index

    Rodrigues, Carina; Costa, Elísio; Vieira, Emília; Carvalho, João; Santos, Rosário; Rocha-Pereira, Petronila; Santos-Silva, Alice; Bronze-da-Rocha, Elsa
    In Press

  6. Mutation screening of the thyroid peroxidase gene in a cohort of 55 Portuguese patients with congenital hypothyroidism.

    Rodrigues, Carina; Jorge, Paula; Soares, José Pires; Santos, Isaura; Salomão, Regina; Madeira, Manuela; Vaz-Osório, Rui; Santos, Rosário
    Defects in the human thyroid peroxidase (TPO) gene are reported to be one of the causes of congenital hypothyroidism (CH) due to a total iodide organification defect. The aim of the present study was to determine the nature and frequency of TPO gene mutations in patients with CH, characterised by elevated TSH levels and orthotopic thyroid gland, identified in the Portuguese National Neonatal Screening Programme. Subjects and methods: The sample comprised 55 patients, from 53 unrelated families, with follow-up in the endocrinology clinics of the treatment centres of Porto and Lisbon. Mutation screening in the TPO gene (exons 1–17) was performed by single-strand conformational analysis followed by...

  7. Body composition and body image perception in portuguese and spanish college students: comparative study between overweight /obese and normal weight women

    Pereira, Elza; Lacerda, Fernanda; Valador, Natacha; Ferro-Lebres, Vera; Arroyo, Marta
    Objective: To evaluate body image perception and compare with real measures of body composition between overweight/obese and normal weight women college students. Subjects: The reported sample included 600 students from Portugal and Spain. Material and Methods: The anthropometry variables: weight, height, skinfolds, circumferences and body mass index, waist-hip ratio, percentage of body fat and fat-free mass- FFM were compared with reference values. The body image perception and satisfaction were estimated using Somatomorphic Matrix and Body Shape Questionnaire (BSQ34). With the difference between actual and ideal measures was obtained the dissatisfaction through body image. For data analysis was used t-test and Pearson correlation. Results:10,7% of the Spanish sample was overweight/obese. Statistical...

  8. Housing and employment situation, body mass index and dietary habits of heroin addicts in methadone maintenance treatment

    Alves, Daniela; Costa, Ana Filipa; Custódio, Daniela; Natário, Liliana; Ferro-Lebres, Vera; Andrade, Fernando
    Forty-nine heroin addicts in methadone maintenance treatment were evaluated with the aim of studying the anthropometric, nutritional and sociodemographic characteristics of these individuals. The BMI of heroin addicts who live with their spouse/partner is significantly higher compared with other housing situations. Most of the heroin addicts evaluated do not consume the minimum servings of fruits, vegetables and grains recommended by the food pyramid, and their consumption of sweets is high. This study reinforced the need for intervention programmes specifically designed to correct the poor nutritional status and diet of drug users, while considering this to be a major public health issue.

  9. Antioxidant capacity and toxicological evaluation of pterospartum tridentatum flower extracts

    Ferreira, Fernanda M.; Dinis, Lia T.; Azedo, Pedro; Galhano, Cristina; Cardoso, Susana M.; Domingues, M.R.M.; Pereira, Olívia R.; Simões, Anabela; Palmeira, Carlos; Peixoto, Francisco P.
    Pterospartum tridentatum Willk. (prickled broom) is an autochthonous plant, common in Portuguese territory. The yellow flowers are used in traditional medicine, as a potential cure for all body illnesses, mainly for throat irritation treatment or for diabetes, hypertension and hypercholesterolemia therapy.Despite its traditional use, no toxicological assessment has been performed as we know. A high antioxidant activity of P. tridentatum flower water extract was acessed in good agreement with its ESI-MS spectrum that revealed the presence of several flavonoids, as luteolin-O-(O-acetyl)-glucuronide, luteolin-O- glucuronide or isorhamnetin-O-hexoside. Mitocondrial respiratory rates (state 4, state 3 and FCCP-stimulated respiration) and respiratory indexes (respiratory control and P/O ratios) showed no consistent decrease of respiratory and...

  10. Evaluation of the microbiological quality of drinking water in the district of Bragança (Northwest Portugal) throughout a ten-year period (1996–2005), during the implementation of the 1998/83 EC Directive

    Nogueira, António José M.; Cardoso, Manuela; Delgadillo, Ivonne; Almeida, Adelaide
    The microbiological quality of drinking waters was evaluated in northwestern Portugal between 1996 and 2005, according to the guidelines of European Directives of 1980 and 1998. Out of the 21,630 microbiological analyses done, the percentage of treated and nontreated waters analyzed were similar, but the fraction of conforming samples were higher for treated (74.2%) than for nontreated (37.8%) water. The microbiological quality of potable water improved throughout the study period, particularly in the last 2 yr, when drinkable water reached 70%. The increase during the last 2 yr is a result of at least two different causes: an increase in the...

  11. Evaluation of the microbiological quality of drinking water in the district of Bragança (Northwest Portugal) throughout a ten-year period (1996–2005), during the implementation of the 1998/83 EC Directive

    Nogueira, António José M.; Cardoso, Manuela; Delgadillo, Ivonne; Almeida, Adelaide
    The microbiological quality of drinking waters was evaluated in northwestern Portugal between 1996 and 2005, according to the guidelines of European Directives of 1980 and 1998. Out of the 21,630 microbiological analyses done, the percentage of treated and nontreated waters analyzed were similar, but the fraction of conforming samples were higher for treated (74.2%) than for nontreated (37.8%) water. The microbiological quality of potable water improved throughout the study period, particularly in the last 2 yr, when drinkable water reached 70%. The increase during the last 2 yr is a result of at least two different causes: an increase in the...

  12. Índices plaquetários em doentes infectados com o vírus da imunodeficiência humana e com trombocitopeniaÍndices plaquetários em doentes infectados com o vírus da imunodeficiência humana e com trombocitopenia

    Pinto, Carla; Barros, Clara; Costa, Elísio
    Existem poucos estudos sobre alterações nos índices plaquetários em doentes trombocitopénicos infectados com o Vírus da Imunodeficiência Humana (VIH). O objectivo deste trabalho foi o de procurar alterações nos índices plaquetários e determinar o grau de correlação destes parâmetros entre si e com os restantes parâmetros hematológicos. PLANO DE ESTUDO: Estudaram-se 49 indivíduos com VIH e trombocitopenia, com idades compreendidas entre os 20 e os 64 anos, de ambos os sexos, e 49 indivíduos controlos. A todos se efectuou hemograma completo, incluindo os índices plaquetários: volume plaquetário médio (MPV), distribuição do tamanho das plaquetas (PDW), plaquetócrito (PCT) e percentagem de plaquetas gigantes (PLCR). RESULTADOS: Na população de...

  13. Índices plaquetários em doentes infectados com o vírus da imunodeficiência humana e com trombocitopeniaÍndices plaquetários em doentes infectados com o vírus da imunodeficiência humana e com trombocitopenia

    Pinto, Carla; Barros, Clara; Costa, Elísio
    Existem poucos estudos sobre alterações nos índices plaquetários em doentes trombocitopénicos infectados com o Vírus da Imunodeficiência Humana (VIH). O objectivo deste trabalho foi o de procurar alterações nos índices plaquetários e determinar o grau de correlação destes parâmetros entre si e com os restantes parâmetros hematológicos. PLANO DE ESTUDO: Estudaram-se 49 indivíduos com VIH e trombocitopenia, com idades compreendidas entre os 20 e os 64 anos, de ambos os sexos, e 49 indivíduos controlos. A todos se efectuou hemograma completo, incluindo os índices plaquetários: volume plaquetário médio (MPV), distribuição do tamanho das plaquetas (PDW), plaquetócrito (PCT) e percentagem de plaquetas gigantes (PLCR). RESULTADOS: Na população de...

  14. IL-7 serum levels and lymphopenia in hemodialysis patients, non-responders to recombinant human erythropoietin therapy

    Costa, Elísio; Lima, Margarida; Rocha, Susana; Rocha-Pereira, Petronila; Castro, Elisabeth; Miranda, Vasco; Sameiro-Faria, Maria; Loureiro, Alfredo; Quintanilha, Alexandre; Belo, Luís; Santos-Silva, Alice

  15. Determination of neutrophil FC receptor IIIB antigens (HNA-1A, HNA-1B AND HNA1C) by ploymerase chain reaction with sequence-specific fluorochrome-labeled primers

    Costa, Elísio; Antunes, Marika Bini; Faria, Susana; Vieira, Emília; Branca, Rosa; Barbot, José; Santos, Rosário

  16. Co-existance of congenital red cell pyruvate kinase and band 3 deficiency

    Branca, Rosa; Costa, Elísio; Rocha, Susana; Coelho, Henrique; Quintanilha, Alexandre; Cabeda, José; Santos-Silva, Alice; Barbot, José

  17. Banda 3 profile as a marker of oxidative stress in hereditary spherocytosis

    Granjo, Elisa; Santos-Silva, Alice; Rebelo, Irene; Nóvoa, Ana; Costa, Elísio; Barbot, José; Ribeiro, Manuela; Quintanilha, Alexandre

  18. Agentes etiológicos e contaminantes em hemoculturas

    Silva, Joana; Ferreira, Susana; Costa, Elísio; Resende, Ana; Ramos, Maria Helena
    A presença de patogénios no sangue, de indivíduos doentes, é sinónimo de taxas elevadas de morbilidade e mortalidade. A cultura de sangue tornou-se um dos testes mais frequentemente praticados nos laboratórios de Microbiologia, quando infecções primárias localizadas resultam na invasão da corrente sanguínea. Com este estudo, pretendeu-se analisar os resultados das hemoculturas estudadas, identificando os principais agentes etiológicos de infecção e principais contaminantes. Foram estudadas 12318 hemoculturas provenientes dos serviços de Internamento e Urgência do Hospital Geral de Santo António, E.P.E, colhidas, no período de Janeiro a Dezembro de 2006. Estas foram agrupadas como hemoculturas negativas, hemoculturas positivas e hemoculturas positivas devido a contaminação. Os microrganismos isolados foram agrupados em agentes etiológicos e...

  19. Agentes etiológicos e contaminantes em hemoculturas

    Silva, Joana; Ferreira, Susana; Costa, Elísio; Resende, Ana; Ramos, Maria Helena
    A presença de patogénios no sangue, de indivíduos doentes, é sinónimo de taxas elevadas de morbilidade e mortalidade. A cultura de sangue tornou-se um dos testes mais frequentemente praticados nos laboratórios de Microbiologia, quando infecções primárias localizadas resultam na invasão da corrente sanguínea. Com este estudo, pretendeu-se analisar os resultados das hemoculturas estudadas, identificando os principais agentes etiológicos de infecção e principais contaminantes. Foram estudadas 12318 hemoculturas provenientes dos serviços de Internamento e Urgência do Hospital Geral de Santo António, E.P.E, colhidas, no período de Janeiro a Dezembro de 2006. Estas foram agrupadas como hemoculturas negativas, hemoculturas positivas e hemoculturas positivas devido a contaminação. Os microrganismos isolados foram agrupados em agentes etiológicos e...

  20. Inflammation, T-Cell phenotype, and inflammatory cytokines in chronic kidney disease patients under hemodialysis and its relationship to resistance to recombinant human erythropoietin therapy

    Costa, Elísio; Lima, Margarida; Alves, João; Rocha, Susana; Rocha-Pereira, Petronila; Castro, Elisabeth; Miranda, Vasco; Faria, Maria Sameiro; Loureiro, Alfredo; Quintanilha, Alexandre; Belo, Luís; Santos-Silva, Alice
    Background Resistance to recombinant human erythropoietin (rhEPO) occurs in some chronic kidney disease (CKD) patients, which may be due to enhanced systemic inflammatory response and to the erythropoiesis-suppressing effect of pro-inflammatory cytokines, some of which are produced by T cells. Aim of study The aim of this study was to investigate the relationship between resistance to rhEPO therapy in hemodialysis CKD patients and inflammatory markers [Creactive protein (CRP), soluble interleukin (IL)-2 receptor(sIL2R), and serum albumin levels], blood cell counts, Tcell phenotype, cytokine production by T cells, and serum cytokine levels. Materials and Methods We studied 50 hemodialysis CKD patients, 25 responders and 25 nonresponders...

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