Barros, Lillian; Dueñas, Montserrat; Carvalho, Ana Maria; Ferreira, Isabel C.F.R.; Santos-Buelga, Celestino
This study aimed to analyse phenolic compounds in wild medicinal flowers of Crataegus monogyna, Cytisus multiflorus, Malva sylvestris and Sambucus nigra, by HPLC-DAD-ESI/MS. Flavonols and flavones were the main groups in almost all the studied samples. C. multiflorus sample gave the highest levels of flavonoids, being a chrysin derivative the most abundant flavone. C. monogyna revealed the highest concentration in phenolic acids that were not found in C. multiflorus; 5-O-caffeoylquinic acid was the most abundant phenolic acid found in the first species, being a procyanidin trimer also found. Kaempferol-3-O-rutinoside and quercetin-3-O-rutinoside were the main flavonols present in M. sylvestris and...
Froufe, Hugo J.C.; Abreu, Rui M.V.; Barros, Lillian; Ferreira, Isabel C.F.R.
Several reports indicate that mushrooms have the ability to promote apoptosis in tumour cell lines, but the mechanism of action is not quite well understood. Inhibition of the interaction between Bcl-2 (anti-apoptotic protein) and pro-apoptotic proteins could be an important step that leads to apoptosis. Therefore, the discovery of compounds with the capacity to inhibit Bcl-2 is an ongoing research topic on cancer therapy. Herein, Autodock4 virtual screening was applied to a dataset of 40 low molecular weight compounds present in mushrooms, using 3D Bcl-2 protein structure (PDB:2XA0) as target. Results suggested that steroids mainly ergosta-4,6,8(14),22-tetraen-3-one, lucidenic lactone, cerevisterol, ganoderic...
Heleno, Sandrina A.; Barros, Lillian; Martins, Anabela; Queiroz, Maria João R.P.; Santos-Buelga, Celestino; Ferreira, Isabel C.F.R.
Mushrooms consumption continues to increase due to their functional benefits and presence of
bioactive compounds. Herein, phenolic, polysaccharidic and lipidic fractions of wild mushrooms
from Northeast Portugal (Coprinopsis atramentaria, Lactarius bertillonii, Lactarius vellereus,
Rhodotus palmatus and Xerocomus chrysenteron) were analysed. Protocatechuic, phydroxybenzoic,
p-coumaric and cinnamic acids were found in the phenolic fraction; ramnose,
xylose, fucose, arabinose, fructose, glucose, manose, mannitol, sucrose, maltose and trehalose were
quantified in polysaccharidic fraction; linoleic and stearic (only in Lactarius sp.) acids, and β- and
γ-tocopherols were the main compounds in the lipidic fraction.
Carocho, Márcio; Antonio, Amilcar L.; Barros, Lillian; Bento, Albino; Kałuska, Iwona; Ferreira, Isabel C.F.R.
The objective of this study was to analyse the effect of
electron beam irradiation (0.5, I and 3 kGy) on the
antioxidant activity of Portuguese chestnuts (Castanea
saliva Mill.) using different in vitro assays, such as the 2,2-
diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging
activity, reducing power, inhibition of ~-carotene bleaching
and inhibition of lipid peroxidation using thiobarbituric
acid reactive species (TBARS). Total phenolics were
determined by spectrophotometric assays. Irradiated
samples seemed to preserve phenolics content and revealed
higher antioxidant activity than the control sample. The
most indicated dose to maintain antioxidants content, and to
increase reducing power and lipid peroxidation inhibition
was 1 kGy.
Barros, Lillian; Dueñas, Montserrat; Alves, Carlos Tiago; Silva, Sónia; Henriques, Mariana; Santos-Buelga, Celestino; Ferreira, Isabel C.F.R.
A screening of the antifungal potential of phenolic extract of Cistus ladanifer from Northeast Portugal, against Candida species was performed. The extract was characterized by HPLC-DAD-ESI/MS. Phenolic acids and derivatives, ellagic acid derivatives and flavonoids, such as catechins, flavonols and flavones, were found in the sample. The most abundant group was ellagic acid derivatives in which punicalagin gallate, a derivative of punicalagin attached to gallic acid, was found in highest amount. These compounds could be related to the strong inhibition of C. albicans, C. glabrata and C. parapsilosis growth. Moreover, the best antifungal activity was against C. glabrata, where the...
Vaz, Josiana A.; Tavares, Catarina; Almeida, Gabriela M.; Martins, Anabela; Ferreira, Isabel C.F.R.; Vasconcelos, M. Helena
Mushrooms are part of the sexual life cycle of particular fungi with specific metabolic
pathways, and therefore may contain a largely unexploited source of powerful new pharmaceutical products with potential antitumor properties [1,2]. Furthermore, they
may have potential as functional foods. Suillus collinitus is an edible mushroom found
in European pine forests. The aim of this work was to study the cytotoxic potential of
extracts from this mushroom in various cancer cell lines.
Different extracts (methanolic, ethanolic and aqueous) were prepared and extractinduced
cell growth inhibition was assessed with the sulforhodamine B assay in four
human tumour cell lines (lung, breast, colon and gastric cancer). The methanolic
Baptista, Paula; Martins, Anabela; Lino-Neto, Teresa; Tavares, Rui Manuel
European chestnut trees (Castanea sativa Mill.) are of great economic importance for wood and fruit production in the northeast of Portugal. In order to maintain the soil free of weeds, farmers traditionally practice several superficial tillages along the year. Phytosanitary problems mainly related with increasing Phytophtora cinnamomi contamination of soils are progressively changing these practices.
Barreira, João C.M.; Ferreira, Isabel C.F.R.; Oliveira, M.B.P.P.; Pereira, J.A.
Reactive oxygen species are known to be implicated in many cell
disorders and in the development of many diseases including cardiovascular diseases atherosclerosis, cataracts, chronic inflammation or
neurodegenerative diseases such as Alzheimer’s or Parkinson 's disease.
Thus, synthetic antioxidants are widely used in the food industry, bur,
because of their toxic and carcinogenic effects, their use is being
Barros, Lillian; Baptista, Paula; Correia, Daniela M.; Ferreira, Isabel C.F.R.
Reactive oxygen and free radicals play an important role in cellular
injury and the ageing process and also are considered to induce the lipid
peroxidation that causes the deterioration of foods. Although organisms
have endogenous antioxidant defenses produced during normal cell
aerobic respiration against the reactive oxygen species, other antioxidants
are taken from the diet, both from natural or synthetic origin.
Ferreira, Isabel C.F.R.; Barros, Lillian; Falcão, Soraia; Vilas-Boas, Miguel; Queiroz, Maria João R.P.
The skin provides the first line of defense against oxidative damage
induced by environmental factors, having an elaborated antioxidant
system designed to deal with free radicals and oxidative stress. However,
under severe stress conditions this biological response is not sufficient,
leading to oxidative damage and, in consequence, to skin disorders,
immunosuppresion, premature skin aging and ultimately cancer.
Ferreira, Isabel C.F.R.; Vilas-Boas, Miguel; Falcão, Soraia; Queiroz, Maria João R.P.
The search for new molecules with antioxidant properties is a very
active domain of research , since they can protect the human body
from free radicals and retard the progress of many chronic diseases,
such a. vascular diseases, some forms of cancer and oxidative stress
responsible for D NA, protein and membrane damage.
Abreu, Rui M.V.; Ferreira, Isabel C.F.R.; Queiroz, Maria João R.P.
Synthetic antioxidants are widely used in food industry y, but because of
toxic and carcinogenic effects revealed by some compounds such as
BHA and BHT, their use is being restricted. The pursuit for novel
compounds with antioxidant properties gained higher significance,
since these compounds may contribute for the prevention of diseases in
which free radicals are implicated.
Heleno, Sandrina A.; Barros, Lillian; Sousa, Maria João; Martins, Anabela; Ferreira, Isabel C.F.R.
ln the last years nineteen different mushrooms species from Northeast
Portugal, one of the European regions with higher , wild mushrooms
diversity, were evaluated, by our team, for their antioxidant properties,
in order to valorise them as a source of nutraceuticals [I]. Nevertheless,
assuming that the proportion of used mushrooms among the undiscovered
and unexamined ones is only 5%, there are thousands of
species with possible benefit for mankind. Therefore, we intend to go
on in the study of this matrix, documenting their nutraceutical potential
and retaining the information for a better management and conservation of this natural resource and related habitats.
Pires, Susana; Pereira, Ermelinda; Lopes-da-Silva, M.F.
The aim of this work is to present a preliminary study for the use of ceramic materials in packaging of natural mineral waters. Handmade ceramic bottles were produced from faience paste and tested for the influence of different glazing areas on the tightness of the ceramic bottles and in physic-chemical stability of bottled natural mineral water during storage (8 days, 15º C). Three ceramic bottles were totally glazed (TG) in both the inner and the outer surface, and other nine bottles were only partially glazed (PG) in the outer surface. Glass bottles were used as control (C). Results showed high...
Carvalho, Ana Maria