Pereira, Ana Rita; Vieira, Duarte Nuno; Magalhães, Teresa
Intimate partner violence (IPV) is an important cause of women's health and socio-familial severe problems, the most extreme being the victims' homicide. This is the first nationwide Portuguese autopsy-based and judicial-proven study about female intimate partner homicide.
At least 62 women over 15 years old were killed by current or former men-intimate partners, corresponding to an IPV-related female mortality rate of 0.44/100.000 women; intimate partner violence was the reason of homicide in 60.8% of all autopsied women.
The typical Portuguese victim showed to be a young adult woman, employed, killed by a current husband in a long-term relationship, usually with children in...
Sena, Cristina M.; Pereira, Ana M.; Seiça, Raquel
The vascular endothelium is a multifunctional organ and is critically involved in modulating vascular tone and structure. Endothelial cells produce a wide range of factors that also regulate cellular adhesion, thromboresistance, smooth muscle cell proliferation, and vessel wall inflammation. Thus, endothelial function is important for the homeostasis of the body and its dysfunction is associated with several pathophysiological conditions, including atherosclerosis, hypertension and diabetes. Patients with diabetes invariably show an impairment of endothelium-dependent vasodilation. Therefore, understanding and treating endothelial dysfunction is a major focus in the prevention of vascular complications associated with all forms of diabetes mellitus. The mechanisms of...
Fernandes, Rosa; Carvalho, Ana Luisa; Kumagai, Arno; Seica, Raquel; Hosoya, Ken-Ichi; Terasaki, Tetsuya; Murta, Joaquim; Pereira, Paulo; Faro, Carlos
Purpose: To investigate the effect of chronic hyperglycemia on the levels of the glucose transporter GLUT1 in retina and
Methods: Two diabetic animal models (Goto Kakizaki rats and alloxan-induced diabetic rabbits) and retinal endothelial
cells in culture were used. GLUT1 content was determined by western blotting. Glut1 mRNA was determined by RT-PCR
and northern blotting. Ubiquitin conjugates were evaluated by western blot analysis. In vitro ubiquitin conjugation activ-
ity was evaluated in supernatants using radiolabeled ubiquitin. Evidence for GLUT1 ubiquitinylation was further investi-
gated by transfecting HEK293 cells with a hemagglutinin (HA)-tagged ubiquitin cDNA followed by immunoprecipitation
of the cell lysates.
Results: Chronic hyperglycemia resulted in...
Freitas, Mariana; Alves, Vera; Sarmento-Ribeiro, Ana; Mota-Pinto, Anabela
Prostate cancer is the most common form of cancer affecting men in the Western world. Risk factors include ageing, genetics, recurrent inflammation, lifestyle and diet intake, related to an increase of oxidative stress. Prostate cancer risk is also associated with exposure to carcinogen such as polycyclic aromatic hydrocarbons (PAHs), originated from the incomplete combustion of carbon-containing fuels like tobacco, wood, diesel, or charbroiled meat. Although numerous studies have associated the effect of PAHs to tumour development, few investigations have associated its effects to cancer progression. Considering that prostate cancer patients don’t die from localized prostate cancer but from advanced disease,...
Natu, Mădălina V.; Gaspar, Manuel N.; Ribeiro, Carlos A. Fontes; Correia, Ilídio J.; Silva, Daniela; Sousa, Hermínio C. de; Gil, M. H.
Implantable dorzolamide-loaded discs were prepared by blending poly(ε-caprolactone), PCL, with poly(ethylene oxide)-b-poly(propylene oxide)-b-poly(ethylene oxide), Lu. By blending, crystallinity, water uptake and mass loss were modified relative to the pure polymers. Burst was diminished by coating the discs with a PCL shell. All samples presented burst release except PCL-coated samples that showed controlled release during 18 days. For PCL-coated samples, barrier control of diffusion coupled with partition control from the core slowed down the release, while for 50/50 Lu/PCL-coated samples, the enhancement in the porosity of the core diminished partition control of drug release. Nonlinear regression analysis suggested that a degradation...
Rosa, S. C.; Rufino, A. T.; Judas, F.; Tenreiro, C.; Lopes, M. C.; Mendes, A. F.
Chondrocytes respond to insulin, but the presence and role of the specific high affinity insulin receptor (InsR) has never been demonstrated. This study determined whether human chondrocytes express the InsR and compared its abundance and function in normal and osteoarthritis (OA) human chondrocytes.
Cartilage sections were immunostained for detection of the InsR. Non-proliferating chondrocyte cultures from normal and OA human cartilage were treated with 1 nM or 10 nM insulin for various periods. InsR, insulin-like growth factor receptor (IGFR), aggrecan and collagen II mRNA levels were assessed by real time RT-PCR. InsR, glucose transporter (GLUT)-1, phospho-InsRbeta and phospho-Akt were evaluated by...
Baptista, Filipa I.; Gaspar, Joana M.; Cristóvão, Armando; Santos, Paulo F.; Köfalvi, Attila; Ambrósio, António F.
Diabetes induces changes in neurotransmitter release in central nervous system, which
depend on the type of neurotransmitter and region studied. In this study, we evaluated
the effect of diabetes (two and eight weeks duration) on basal and evoked release of
[14C]glutamate and [3H]GABA in hippocampal and retinal synaptosomes. We also
analyzed the effect of diabetes on the protein content of vesicular glutamate and GABA
transporters, VGluT-1, VGluT-2 and VGAT, and on the α1A subunit of P/Q type calcium
channels, which are abundant in nerve terminals.
The protein content of vesicular glutamate and GABA transporters, and of the α1A
subunit, was differently affected by diabetes in hippocampal and retinal...
Santos, R. A.; Nogueira, C. S.; Baptista, J. B; Granja, S.; Ribeiro, M. L.; Rocha, M. G.
Kawasaki disease (KD) is an acute febrile multisystem vasculitic syndrome of unknown etiology, occurring mostly in infants and children younger than 5 years of age. We present a 13-month-old male with KD from whom was found human bocavirus DNA in nasopharyngeal secretions. Human bocavirus DNA in a patient with KD raised question about the coincidental or possible etiological association.
Mota-Pinto, Anabela; Todo-Bom, Ana; Alves, Vera; Santos, Alexandra; Santos-Rosa, Manuel
Background: Airway walls in asthma present an accumulation of activated cells that determine bronchial structural changes and disease progression and severity. During the aging process, the immunoinflammatory response changes as a consequence of chronic antigenic stress.
Objective: To evaluate T-cell subsets with regulatory functions associated with asthma in elderly patients.
Methods: A group of 153 individuals (95 with controlled asthma and 58 healthy controls) aged over 65 years was studied. Blood samples were collected for fl ow cytometry analyses of CD3, CD4, CD8, CD56, CD56CD8, CD3CD4CD25, CD3CD4CD25CD127, CD4HLA-DR and TCRγδ.
Results: Asthmatic patients showed a statistically significant increase in CD4+ T cells. CD3CD4CD25high...
Baiardini, I; Braido, F; Bindslev-Jensen, C; Bousquet, PJ; Brzoza, Z; Canonica, GW; Compalati, E; Fiocchi, A; Fokkens, W; Gerth van Wijk, R; Giménez-Arnau, A; Godse, K; Grattan, C; Grob, JJ; La Grutta, S; Kalogeromitros, D; Kocatürk, E; Lombardi, C; Mota-Pinto, A; Ridolo, E; Saini, SS; Sanchez-Borges, M; Senna, GE; Terreehorst, I; Todo-Bom, A; Toubi, E; Bousquet, J; Zuberbier, T; Maurer, M
The aim of this Global Allergy and Asthma European Network (GA2LEN) consensus report is to provide recommendations and suggestions for assessing patient-reported outcomes (PROs) including health-related quality of life in patients with urticaria. We recommend that PROs should be used both in clinical trials and routine practice for the evaluation of urticaria patients. We suggest that PROs should be considered as the primary outcome of future clinical trials. Two validated and disease-specific instruments for assessing PROs are available, the urticaria activity score (for symptoms) and the chronic urticaria questionnaire on quality of life CU-Q2oL. This latter tool, CU-Q2oL, is available...
Baiardini, I; Bousquet, PJ; Brzoza, Z; Canonica, GW; Compalati, E; Fiocchi, A; Fokkens, W; Gerth van Wijk, R; La Grutta, S; Lombardi, C; Maurer, M; Mota-Pinto, A; Ridolo, E; Senna, GE; Terreehorst, I; Todo-Bom, A; Bousquet, J; Zuberbier, T; Braido, F
The aim of this GA2LEN consensus report is to provide recommendations for Patient-Reported Outcomes evaluation in clinical trials for allergic diseases, which constitute a global health problem in terms of physical, psychological, economic and social impact. During the last 40 years PROs have gained large consideration and use in the scientific community, in order to gain a better understanding of patients’ subjective assessment with respect to elements concerning their health condition. They include all health-related reports coming from the patient, without involvement or interpretation by physician or others.
PROs assessment should be performed by validated tools (disease-specific tools when available, or...
Braido, F; Bousquet, PJ; Brzoza, Z; Canonica, GW; Compalati, E; Fiocchi, A; Fokkens, W; Gerth van Wijk, R; La Grutta, S; Lombardi, C; Maurer, M; Mota-Pinto, A; Ridolo, E; Senna, GE; Terreehorst, I; Todo-Bom, A; Bousquet, J; Zuberbier, T; Baiardini, I
The GA2LEN taskforce on Patient-Reported Outcomes (PROs) and Health-Related Quality of Life (HRQoL) published in 2009 a position paper concerning PROS and HRQoL assessment in clinical trials on allergy. Because of the specificity of this topic in asthma and rhinitis, specific recommendations are needed. The aim of this position paper is to define PROs and their meaning in asthma and rhinitis research, explore the available tools to provide criteria for a proper choice, identify patientrelated factor which could influence PROs assessment, define specific recommendations for assessment, analysis and results spreading, underline the unexplored areas and unmet needs. PROs assessment is...
Todo-Bom, Ana; Mota-Pinto, Anabela; Vale-Pereira, Sofia; Alves, Vera; Dourado, Marília; Santos-Rosa, Manuel
Background: Substance P (SP) was described at the beginning of the 20th Century, and its biological action was recognized to have implications in neurogenic inflammation and constriction of smooth muscles. The changes associated with inflammatory chronicity can compromise organ function reversibility. The role of neuromechanisms in the pathology of the disease has been investigated in order to achieve better diagnosis and therapeutic approaches. The stimulation of human cells, such as macrophages and polymorphonuclear cells by SP leads to their activation and to the release of reactive oxygen species (ROS) by these cells. Consequently, a continuous inflammatory disability is observed, mainly...
Vale-Pereira, Sofia; Todo-Bom, Ana; Geraldes, Luisa; Schmidt-Weber, Carsten; Akdis, Cesmi; Mota-Pinto, Anabela
Background Asthma is a chronic inflammatory disorder in which Th2, Th1 and suppressive T
cells (Tregs) play a role. The transcription factor FoxP3 plays a role in Treg differentiation hile T-bet is important for Th1 and GATA-3 for Th2 differentiation from naïve T cells. Recent data show that age-related deregulation of Treg cells is a mechanism of senescence affecting several chronic diseases. It is crucial to understand the behaviour of these cell populations in asthma for elderly patients.
Objective To evaluate FoxP3, GATA-3 and T-bet gene expression under basal conditions and after in vitro activation in a group of elderly asthmatic compared...
Todo-Bom, Ana; Mota-Pinto, Anabela
A história natural da asma e as condições determinantes de evolução para formas moderadas ou graves não estão completamente estabelecidas. Contudo, quer os fatores genéticos quer os fatores ambientais serão determinantes na fisiopatologia e no prognóstico da doença. Nesta revisão são apresentados os mecanismos envolvidos na fisiopatologia da asma grave
Mota-Pinto, Anabela; Todo-Bom, Ana; Vale-Pereira, Sofia; Alves, Vera; Santos-Rosa, Manuel
Neopterin is synthesized by human monocyte-derived macrophages upon stimulation with interferon- (IFN- ). Measurement of neopterin concentration is useful to monitor cell-mediated (Th1-type) immune activation. In this study, we aimed to analyze the behaviour of neopterin in long lasting asthma considering its role as a marker of the Th1 environment and to establish the distinction between patients belonging either to the allergic or the non-allergic population, particularly in the elderly where asthma is often under diagnosed. Therefore we evaluated allergic parameters such as skin prick tests, IgE and hemogram (eosinophils count), and we compared our findings with neopterin values found...
Freitas, Mariana; Alves, Vera; Sarmento-Ribeiro, Ana Bela; Mota-Pinto, Anabela
Docetaxel and sodium selenite are well known for their anticancer properties. While resistance to docetaxel remains an obstacle in prostate cancer chemotherapy, sodium selenite, has been exploited as a new therapeutic approach. Currently, development of therapies affecting a multitude of cell targets, have been proposed as a strategy to overcome drug resistance. This association may reduce systemic toxicity counteracting a wide range of side effects.
Here we report the effect of docetaxel and sodium selenite combination on the PC3 prostate cancer cell line, derived from bone metastasis. Therefore we evaluate cell growth, cell cycle progression, viability, mitochondria membrane potential, cytochrome C,...
Freitas, Mariana; Baldeiras, Inês; Proença, Teresa; Pascoal, Rui; Rabaça, Carlos; Valido, Frederico; Mota-Pinto, Anabela; Sarmento-Ribeiro, Ana Bela
Cigarette smoke is an oxidant, creating free radicals that are involved in carcinogenesis namely in Prostate Cancer (PC). However, a correlation between cigarette smoking and PC progression remains unclear.
This study was directed to evaluate the correlation between cigarette smoking, Oxidative Stress (OS) and PC development and progression. The Total Antioxidant Status (TAS), Glutathione (GSH) and Malondialdehyde (MDA) were analysed in the plasma and/or in the erythrocytes of PC patients, 5 smokers and 9 non-smokers and in controls, 2 smokers and 4 non-smokers.
Our results show a significantly decrease in TAS and GSH, and an increase in MDA levels, in PC smoker...
Dourado, Marília; Sarmento-Ribeiro, Ana Bela; Vale-Pereira, Sofia; Alves, Vera; Silva, Teresa; Mota-Pinto, Anabela; Santos-Rosa, Manuel
Dipeptidyl peptidase IV, a cell membrane surface protease also known as CD26 (CD26/DPPIV), is known to play multiple functions in human organism, where it is largely expressed, for instance, in the development of human cancer and metastasis as well as in chemotherapy response.
The objective of this work was to study the CD26 membrane expression and DPPIV activity in T-acute leukaemia cell lines (CEM and MOLT3) in culture, in order to observe the modification of its expression under the 8-azaguanine treatment. Cell line samples were incubated, some without different azaguanine concentration and others with, ranging from 10 to 100µM. Cell surface...
Dourado, Marília; Alves, Vera; Mesquita, Luis; Ramos, Isabel; Mota-Pinto, Anabela; Santos-Rosa, Manuel
The prevention of hepatitis B is important, since it is responsible for significant morbidity and mortality around the world. Unfortunately, hepatitis B vaccine does not always induce protective immunity. The lack of immune response to vaccine (non-responders) can depend on individual characteristics.
The objective of this study was to correlate the CD26/DPPIV cellular expression and DPPIV serum activity with HBV vaccine response and its possible role as an indicator of immune competence acquisition.We also determined the cellular expression of CD3, CD19, CD56 and CD25 in peripheral blood T lymphocytes.
Blood samples were obtained from 28 healthy human volunteers who were enrolled with...