Fernandes, Rosa; Carvalho, Ana Luisa; Kumagai, Arno; Seica, Raquel; Hosoya, Ken-Ichi; Terasaki, Tetsuya; Murta, Joaquim; Pereira, Paulo; Faro, Carlos
Purpose: To investigate the effect of chronic hyperglycemia on the levels of the glucose transporter GLUT1 in retina and
Methods: Two diabetic animal models (Goto Kakizaki rats and alloxan-induced diabetic rabbits) and retinal endothelial
cells in culture were used. GLUT1 content was determined by western blotting. Glut1 mRNA was determined by RT-PCR
and northern blotting. Ubiquitin conjugates were evaluated by western blot analysis. In vitro ubiquitin conjugation activ-
ity was evaluated in supernatants using radiolabeled ubiquitin. Evidence for GLUT1 ubiquitinylation was further investi-
gated by transfecting HEK293 cells with a hemagglutinin (HA)-tagged ubiquitin cDNA followed by immunoprecipitation
of the cell lysates.
Results: Chronic hyperglycemia resulted in...
Curate, Francisco; Piombino-Mascali, Dario; Tavares, Ana; Cunha, Eugénia
Matos, Vítor M. J.; Santos, Ana Luísa
Leprogenic odontodysplasia (LO), also known as dens leprosus, consists of anomalous root
development of the permanent upper incisors. This dental anomaly was first reported by Danielsen in 1968 among Danish juvenile skeletons from medieval leprosaria cemeteries. As yet, no clinical cases have been documented and the etiological and epidemiological significance of the condition are poorly understood. The aim of this study is to discuss a case of LO found amongst the skeletons from the St. Jørgen’s leprosarium cemetery (13th–16th/17th centuries), housed in the ADBOU (Anthropological Database of Odense University), Southern Denmark University. A juvenile individual presents a disarticulated maxillary right central...
Fernandes, Daniel M.; Silva, Ana M.; Donnabhain, Barra O’; Pinhasi, Don
Most studies of dental microevolution have used the standard methodologies employed in
dental anthropology: buccolingual/mesiodistal lengths and the frequencies of non-metric dental traits.
In this work we use the occlusal polygon method which is based on a polygon created by linking the four molar cusp apices using digital analysis. This method has been used to identify different evolutionary trends in Neandertal and modern humans; our objective was to assess the existence of changes
in the occlusal polygon area, and thus the general morphology of first upper molars, between two Portuguese samples from the Late Neolithic (4130 ± 90 BP) and the early 20th...
Santos, A. L.; Gardner, M. T.; Allsworth-Jones, P.
The first inhabitants of Jamaica are now generally referred to as Taínos. It is likely that they arrived
in the island after about 650 AD and were extinct by the end of the 16th century. In 1968, during the
exploration of a small cave in Bull Savannah, St. Elizabeth parish, Dr. James Lee found two skulls,
teeth, bones, and pottery. The aim of this work is to interpret in a biocultural perspective the one
cranium with pathological lesions, such as caries sicca. This adult individual had an artificially
modified cranium, a cultural practice common among Taínos, which was studied macroscopically
and by radiological and computerized tomography....
Campanacho, Vanessa; Santos, Ana Luísa
The possible association between entheseal changes and activity has been widely
studied. However many questions remain. This study aims to assess if occupation and
physical activity influences the age at which entheseal changes appear in the iliac crest,
retroauricular area, iliac tuberosity, ischial tuberosity and obturator foramen. Absence
or presence of ossification exostosis and stress lesions was recorded in os coxae from
130 males (19 to 88 years old) from Lisbon and Coimbra identified skeletal collections.
The individuals were divided into two groups, based upon to the recorded occupations:
manual (n = 69) and non-manual (n = 61). The sample was also divided according to an
Baptista, Filipa I.; Gaspar, Joana M.; Cristóvão, Armando; Santos, Paulo F.; Köfalvi, Attila; Ambrósio, António F.
Diabetes induces changes in neurotransmitter release in central nervous system, which
depend on the type of neurotransmitter and region studied. In this study, we evaluated
the effect of diabetes (two and eight weeks duration) on basal and evoked release of
[14C]glutamate and [3H]GABA in hippocampal and retinal synaptosomes. We also
analyzed the effect of diabetes on the protein content of vesicular glutamate and GABA
transporters, VGluT-1, VGluT-2 and VGAT, and on the α1A subunit of P/Q type calcium
channels, which are abundant in nerve terminals.
The protein content of vesicular glutamate and GABA transporters, and of the α1A
subunit, was differently affected by diabetes in hippocampal and retinal...
Dolbeth, M.; Cardoso, P. G.; Grilo, T. F.; Bordalo, M. D.; Raffaelli, D.; Pardal, M. A.
The macrobenthic production of an estuarine system was evaluated over a 14-year study period in a seagrass bed and in a sandflat. Over this period, the estuary suffered severe eutrophication and extreme weather events with important impacts on the community, impairing system functioning and ultimately the goods and services provided by the estuary (decline in the seagrass bed, decreased community production and/or a boost in the production by opportunist species, such as Hydrobia ulvae). Following the anthropogenic impacts, management measures were introduced which allowed a gradual recovery of the seagrass bed and a new macrobenthic community structure manifested by production...
Gomes-da-Silva, Lígia C.; Santos, Adriana O.; Bimbo, Luís M.; Moura, Vera; Ramalho, José S.; Lima, Maria C. Pedroso de; Simões, Sérgio; Moreira, João N.
The present work aimed at designing a lipid-based nanocarrier for siRNA delivery
towards two cell sub-populations within breast tumors, the cancer and the endothelial
cells from angiogenic tumor blood vessels. To achieve such goal, the F3 peptide, which
is specifically internalized by nucleolin overexpressed on both those sub-populations,
was used as a targeting moiety.
The developed F3-targeted stable nucleic acid lipid particles presented adequate features
for systemic administration. In addition, the attachment of the F3 peptide onto the
liposomal surface enabled an internalization by both cancer and endothelial cells from
angiogenic blood vessels that was significantly higher than the one...
Santos, Ana Luísa; Suby, Jorge Alejandro
Osteomyelitis is a non-specific infection of bone and bone marrow. In the past acute
osteomyelitis (AO) led to high mortality especially in non-adults. Nevertheless, its diagnosis
in archaeological populations is rare. Documented individuals with known cause of death
offer a unique opportunity to study this condition. This paper aims to describe the bone
lesions in non-adults diagnosed with AO at the Coimbra University Hospital (CUH) and now
belonging to the Coimbra Identified Skeletal Collection (CISC). Moreover, mortality rates
and demographic profiles for individuals aged ≤18 years old and diagnosed with AO in the
CUH, between 1923 and 1929, were also determined. The 5 (1%) non-adults in the...
Curate, Francisco; Assis, Sandra; Lopes, Célia; Silva, Ana Maria
Fractures are ubiquitous in the archaeological record but the majority of these are the consequence of a traumatic incident and do not reflect any loss of strength inherent to the bone. So-called
fragility fractures, particularly hip fractures, are considered uncommon occurrences in skeletal populations from the past. Nevertheless, evidence of this type of fracture in the archaeological record is
increasing. A methodical search for possible hip fractures in the excavation reports, theses and monographs housed in the Department of Anthropology of the University of Coimbra presented an occasion
to describe six hip fractures, previously unpublished, from different Portuguese archaeological sites
and to challenge the...
Umbelino, Cláudia; Santos, Ana Luísa; Assis, Sandra
Identified osteological collections are important sources for pathological studies, especially
when the causes of death of the individuals are known. However, the individual may have suffered
from other disorders, not related to the death and thus absent from written record of death. The aims
of this paper are to describe the pathological signs perceptible on a skeleton, and to consider the possible
aetiologies beyond the recorded cause of death. Skeleton number 470 belongs to the Identified
Skeletal Collection of the University of Coimbra, Portugal. This female died in 1933, and pulmonary
tuberculosis was recorded as the cause of death. The skeleton was observed macroscopically by the
Primo, Ana Lígia; Azeiteiro, Ulisses Miranda; Marques, Sónia Cotrim; Pardal, Miguel Ângelo
Recent variations in the precipitation regime across southern Europe have led to changes in river fluxes and salinity gradients affecting biological communities in most rivers and estuaries. A sampling programme was developed in the Mondego estuary, Portugal, from January 2003 to December 2008 at five distinct sampling stations to evaluate spatial, seasonal and inter-annual distributions of fish larvae. Gobiidae was the most abundant family representing 80% of total catch and Pomatoschistus spp. was the most important taxon. The fish larval community presented a clear seasonality with higher abundances and diversities during spring and summer seasons. Multivariate analysis reinforced differences among...
Tavares, Ana Cristina
Estória infantil que pretende demonstrar as diferentes etapas evolutivas que deram origem à flora actual.
Tavares, Ana Cristina
An educative program was initiated in 1997 at the Botanical Garden of the University of Coimbra. using the fundamental principles for the environmental education, volunteer's high school students are annually trained. Guided visits and sprcifc seasonally activities under different themes are prepared for school groups according to their curricula and also for general public.
Tavares, Ana Cristina; Gonçalves, Maria José; Cavaleiro, Carlos; Cruz, Maria Teresa; Lopes, Maria Celeste; Canhoto, Jorge; Ribeiro Salgueiro, Ligia
Ethnopharmacological relevance: Essential oils are known to possess antimicrobial activity against a wide spectrum of bacteria and fungi. Daucus carota L. is used since olden times in traditional medicine, due to recognized therapeutic properties, namely the antimicrobial activity of their essential oils.
Aim of the study: In the present study the composition and the antifungal activity of the oils of Daucus carota L. subsp. halophilus (Brot.) A. Pujadas (Apiaceae), an endemic plant from Portugal, were evaluated. Moreover, their cytotoxicity in mouse skin dendritic cells at concentration showing significant antifungal activity was also evaluated. Material and methods: The oilswere investigated by GC...
Tavares, Ana Cristina; Salgueiro, Lígia R.; Canhoto, Jorge M.
Daucus carota subsp. halophilus, is a wild crop
relative of domestic carrot. It is an aromatic plant widely used in folk medicine due to recognized therapeutic properties of its essential oils. Experiments were carried out to evaluate the potential of in vitro propagation techniques to the conservation of this endemic and endangered taxon. The results showed that shoot tips of in vitro germinated seeds were able to proliferate in the presence of benzyladenine, with the best results being
achieved using 4.4μM, both in the first and second cultures. Shoots rooted after being transferred to 1/2- Murashige and Skoog basal medium. The results...
Tavares, Ana Cristina; Gonçalves, Maria José; Cruz, Maria Teresa; Cavaleiro, Carlos; Lopes, Maria Celeste; Canhoto, Jorge; Ribeiro Salgueiro, Ligia
Ethnopharmacological relevance: Several aromatic plants and their essential oils are known to possess
antimicrobial and anti-inflammatory properties. Distichoselinum tenuifolium (Lag.) Garcia Martin
& Silvestre, an Iberian endemism, is traditionally used in the treatment of contact dermatitis and skin
infections. However, the cellular mechanisms through which this plant exerts their beneficial effects are
Aim of the study: The aims of this study were to examine the chemical composition of the essential oil
isolated from Distichoselinum tenuifolium, and to test the efficacy of the essential oil as an antifungal and
Materials and methods: The oils were investigated by gas chromatography (GC) and gas
chromatography–mass spectrometry (GC–MS) and...
Sousa, Ana Paula; Amaral, Alexandra; Baptista, Marta; Tavares, Renata; Caballero Campo, Pedro; Caballero Peregrín, Pedro; Freitas, Albertina; Paiva, Artur; Almeida-Santos, Teresa; Ramalho-Santos, João
Human sperm samples are very heterogeneous and include a low amount of truly functional gametes. Distinct strategies
have been developed to characterize and isolate this specific subpopulation. In this study we have used fluorescence
microscopy and fluorescence-activated cell sorting to determine if mitochondrial function, as assessed using mitochondrialsensitive
probes, could be employed as a criterion to obtain more functional sperm from a given ejaculate. We first
determined that mitochondrial activity correlated with the quality of distinct human samples, from healthy donors to
patients with decreased semen quality. Furthermore, using fluorescence-activated cell sorting to separate sperm with active
and inactive mitochondria we found that this was also...
Inácio, Ângela S.; Mesquita, Katia A.; Baptista, Marta; Ramalho-Santos, João; Vaz, Winchil L. C.; Vieira, Otília V.
The need for woman-controlled, cheap, safe, effective, easy-to-use and easy-to-store topical applications for prophylaxis against sexually transmitted infections (STIs) makes surfactant-containing formulations an interesting option that requires a more fundamental knowledge concerning surfactant toxicology and structure-activity relationships.
We report in vitro effects of surfactant concentration, exposure time and structure on the viability of mammalian cell types typically encountered in the vagina, namely, fully polarized and confluent epithelial cells, confluent but non-polarized epithelial-like cells, dendritic cells, and human sperm. Representatives of the different families of commercially available surfactants – nonionic (Triton X-100 and monolaurin), zwitterionic (DDPS), anionic (SDS), and cationic (CnTAB...