DSpace - Universidade do Porto
Este repositório institucional visa coleccionar, preservar e disponibilizar na Internet a produção intelectual da comunidade académica da U.PORTO. Apenas as publicações em texto integral e de acesso livre (sob as condições de licença pública Creative Commons)fazem parte deste repositório. Informações adicionais sobre outras publicações encontram-se em www.up.pt.
Parallel increase in placental oxidative stress and antioxidant defenses occurs in type 1 but not gestational diabetes - Araújo, João; Ramalho, Carla; Branco, Ana; Faria, Ana; Ferraz, Tiago; Keating, Elisa; Martel, Fátima
We aimed to determine the oxidative stress status in placentas obtained from gestational (GDM) and type 1 (T1D) diabetic pregnancies. Malonaldehyde and protein carbonyls, two biomarkers of oxidative damage, were higher in T1D but not in GDM placentas. Also, higher reduced glutathione and lower oxidized glutathione levels and higher glutathione peroxidase activity were found in T1D but not in GDM placentas. These results suggest that T1D placentas may develop a protective antioxidant mechanism to overcome higher oxidative stress levels.
Microbiological quality of ready-to-eat salads: An underestimated vehicle of bacteria and clinically relevant antibiotic resistance genes - Campos, Joana; Mourão, Joana; Pestana, Nazaré; Peixe, Luisa; Novais, Carla; Antunes, Patrícia
The increase demand for fresh vegetables is causing an expansion of the market for minimally processed vegetables
along with new recognized food safety problems. To gain further insight on this topic we analyzed the microbiological
quality of Portuguese ready-to-eat salads (RTS) and their role in the spread of bacteria carrying
acquired antibiotic resistance genes, food products scarcely considered in surveillance studies. A total of 50 RTS
(7 brands; split or mixed leaves, carrot, corn) were collected in 5 national supermarket chains in Porto region
(2010). They were tested for aerobic mesophilic counts, coliforms and Escherichia coli counts as well as for the
presence of Salmonella and...
Intraventricular pressure gradients in heart failure - Guerra, Miguel; Silva, Carmen Brás; Amorim, Mário; Bento, Cláudia; Bastos, Pedro; Moreira, Adelino
The aim of the present study was to characterize intraventricular pressure gradients (IVPGs) in an animal model of chronic heart failure. New Zealand rabbits were treated with doxorubicin (heart failure group, n=5) or saline (control group, n=5) and instrumented with pressure catheters placed in the apex and outflow-tract of left ventricle (LV) and with sonomicrometer crystals placed in the apex and base of the LV free wall. In heart failure animals, ventricular filling was delayed and slower when compared with control animals. Moreover, the physiological nonuniformity observed between apical and basal segments in normal hearts was abolished in failing hearts....
Food and Nutrition Actions and their Interface with Food Security: a comparison between Brazil and Portugal - Vieira, Viviane; Gregório, Maria; Mancuso, Ana; Graça, Pedro
This paper aims to compare food and nutrition actions related to foodsecurity (FS) in Brazil and Portugal, keeping in mind that several countries have established guidelines to guarantee adequate food. Both nations are experiencing a reduction in fertility rates, increased life expectancy, and a high prevalence of non-communicable diseases. In Brazil, 30.2% of households live in food insecurity; in Portugal, even without estimating food insecurity, 18.0% of its inhabitants are at risk for poverty. The National Food and Nutrition Policy is the current Brazilian document which seeks to ensure universal access to food. Portugal, due to its inclusion in the...
BMI and self-perceived body shape in Portuguese children - Coelho, Eduarda; Padez, Cristina; Moreira, Pedro; Rosado, Victor; Carvalhal, Isabel
Objectives: This study examined the relationship between Portuguese children's self-perceived body shape and body mass index (BMI) by gender, and BMI values were determined for each silhouette.
Method: A sample of 4,211 children aged 7-10 was used. Their body image was assessed using Collins's body image silhouettes, while cole et al.'s cutoffs were used to define obesity.
Results: The higher the number of the selected silhouette, the higher the mean BMI, except in the case of silhouettes 2 and 7 for boys and 7 for girls. Most of the individuals who selected silhouette number 5 or higher were obese. For...
Therapeutic potential of neuregulin1 in cardiovascular disease - Ferreira, Pedro; Keulenaer, G.; Moreira, Adelino; Silva, Carmen Brás
Neuregulin-1 (NRG-1)/ErbB signaling has an indispensable role in cardiac development and in the maintenance of the structural and functional integrity of the human adult heart in health and disease. Several animal studies have now demonstrated the therapeutic effects of NRG-1 during acute cardiac injury and during chronic heart failure, with improvements in cardiac performance and animal survival. Phase I and II clinical trials for chronic heart failure in humans are now in progress.
Optimization of QuEChERS Procedure Coupled to GC-ECD for Organochlorine Pesticide Determination in Carrot Samples - Sá, Maria; Fernandes, Virginia; Calhau, Conceição; Domingues, Valentina; Matos, Cristina
An optimised version of the Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) method for simultaneous determination of 14 organochlorine pesticides in carrots was developed using gas chromatography coupled with electron-capture detector (GC-ECD) and confirmation by gas chromatography tandem mass spectrometry (GC-MS/MS). A citrate-buffered version of QuEChERS was applied for the extraction of the organochlorine pesticides, and for the extract clean-up, primary secondary amine, octadecyl-bonded silica (C18), magnesium sulphate (MgSO4) and graphitized carbon black were used as sorbents. The GC-ECD determination of the target compounds was achieved in less than 20 min. The limits of detection were below the EU...
Handgrip strength and associated factors in hospitalized patients - Guerra,R.; Fonseca, I.; Pichel, F.; Restivo, M.; Amaral, Teresa
Background: Handgrip strength (HGS) is a marker of nutrition status. Many factors are associated with HGS. Age, height, body mass
index, number of diagnoses, and number and type of drugs have been shown to modify the association between undernutrition and
HGS. Nevertheless, other patient characteristics that could modify this association and its joint modifier effect have not been studied
yet. Objective: To evaluate the association of inpatients’ HGS and undernutrition considering the potential modifier effect of cognitive
status, functional activity, disease severity, anthropometrics, and other patient characteristics on HGS.
Methods: A cross-sectional study
was conducted in a university hospital. Sex, age,...
Food waste as an index of foodservice quality - Ferreira, Maria; Martins, Margarida; Rocha, Ada
Purpose – The purpose of this paper is to evaluate waste at a Portuguese university setting in order
to suggest improvements on foodservice sustainability.
Design/methodology/approach – The work was developed during four weeks in order to allow
the evaluation of parameters of an entire menu cycle. The index of waste/consumption (%) was
obtained by the ratio between plate waste and the quantity of foods distributed, subtracting the weight
Findings – The average index of waste/consumption was approximately 24 percent, higher in the
days with a fish menu, values considered unacceptable. Each canteen user is served with
approximately 940 g of foods, from which only 660 g...
Early dietary treated patients with phenylketonuria can achieve normal growth and body composition. - Rocha, Júlio; Spronsen, Francjan; Almeida, Manuela; Guimarães, João; Ramos, Elisabete; Borges, Nuno
BACKGROUND: In the past, overtreatment may have resulted in growth impairment in patients with phenylketonuria.
The paper aims to investigate height and body composition in early treated patients with phenylketonuria who were diagnosed between 1981 and 2008.
A cross-sectional study of 89 patients with phenylketonuria and 78 controls aged (mean±SD, in years) 14.4±6.6 and 15.9±7.1, respectively, was undertaken, including anthropometric and body composition evaluation using bioelectrical impedance. Median Phe concentrations in the last year before study enrolment were used as a measure of metabolic control. Natural protein and amino acid mixture intakes were recorded in patients.
No statistically significant differences were found on...
Furans and other volatile compounds in ground roasted and espresso coffee using headspace solid-phase microextraction: Effect of roasting speed - Petisca, Catarina; Palacios, Trinidad; Farrah, Adriana; Pinho, Olívia; Ferreira, Isabel
The profile of major classes of furanic compounds, as well as other volatile compounds, was evaluated by headspace solid-phase microextraction GC/MS in Arabica ground coffee roasted at three speeds, and in their respective espresso coffees. A total of 113 and 105 volatile compounds were respectively identified in ground coffee and espresso coffee. They were clustered in the following chemical classes: furans, pyrroles, pyridines, pyrazines, ketones, hydrocarbons, aldehydes and others. Results from Principal Component Analysis (PCA) using the data of major volatile class as variables showed that the levels (expressed as relative percentage of total peak area) of furans and pyrroles...
Differential responses of adiposity, inflammation and autonomic function to aerobic versus resistance training in older adults - Wanderley, Flavia; Moreira, Andre; Sokhatska, Oksana; Palmares, Carmo; Moreira, Pedro; Sandercock, Gavin; Oliveira, Jose; Carvalho, Joana
Background: Increased body fat, autonomic dysfunction and low-grade chronic inflammation are interrelated risk factors implicated in the etiology of several chronic conditions normally presented by older adults. Objective: This study aims to assess the effectiveness of different training protocols on reducing body fat, improving autonomic function, and decreasing low-grade systemic inflammation in community-dwelling elderly adults.
Methods: Fifty participants (11 men, 68 +/- 5.5 years) were randomly allocated into resistance or aerobic training or control groups. Evaluations were done at baseline and following the 8-month intervention period on their body composition (assessed by DXA), inflammatory biomarkers (high-sensitivity C-reactive protein [hs-CRP], tumor...
R&D DYNAMICS - Becker, J.; Ferreira, M.; Oliveira, Bruno; Pinto, A.
We study a Cournot duopoly model using Ferreira-Oliveira-Pinto's R&D investment function. We find the multiple perfect Nash equilibria and we analyse the economical relevant quantities like output levels, prices, consumer surplus, profits and welfare.
Impact of an intervention through teachers to prevent consumption of low nutrition, energy-dense foods and beverages: a randomized trial - Rosário, Rafaela; Araújo, Ana; Oliveira, Bruno; Padrão, Patrícia; Lopes, Óscar; Teixeira, Vitor; Moreira, André; Barros, Renata; Pereira, Beatriz; Moreira, Pedro
To examine the effects of an intervention program held by teachers previously trained in nutrition, on the consumption of low nutrient, energy-dense (LNED) foods, of children attending elementary schools.
464 children (239 female, 6 to 12 years) from seven elementary Portuguese schools participated in this randomized trial. Three schools were allocated to the intervention, and four to the control group. The intervention program was based on the Health Promotion Model and social cognitive theory. Teachers previously trained by researchers in nutrition and healthy eating implemented the intervention in the classroom from November 2008 to March 2009. Sociodemographic, anthropometric, physical activity, and...
Rodent models of heart failure: an updated review - Gomes, A.; Pires, I.; Pires, A.; Silva, Carmen Brás; Moreira, Adelino
Heart failure (HF) is one of the major health and economic burdens worldwide, and its prevalence is continuously increasing. The study of HF requires reliable animal models to study the chronic changes and pharmacologic interventions in myocardial structure and function and to follow its progression toward HF. Indeed, during the past 40 years, basic and translational scientists have used small animal models to understand the pathophysiology of HF and find more efficient ways of preventing and managing patients suffering from congestive HF (CHF). Each species and each animal model has advantages and disadvantages, and the choice of one model over...
Quercetin and epigallocatechin gallate inhibit glucose uptake and metabolism by breast cancer cells by an estrogen receptor-independent mechanism - Moreira, Liliana; Araújo, Isabel; Costa, Tito; Branco, Ana; Faria, Ana; Martel, Fátima; Keating, Elisa
In this study we characterized H-3-2-deoxy-b-glucose (H-3 -DG) uptake by the estrogen receptor (ER)-positive MCF7 and the ER-negative MDA-MB-231 human breast cancer cell lines and investigated the effect of quercetin (QUE) and epigallocatechin gallate (EGCG) upon H-3-DG uptake, glucose metabolism and cell viability and proliferation.
In both MCF7 and MDA-MB-2.31 cells H-3-DG uptake was (a) time-dependent, (b) saturable with similar capacity (V-max) and affinity (K-m), (c) potently inhibited by cytochalasin B, an inhibitor of the facilitative glucose transporters (GLUT), (d) sodium-independent and (e) slightly insulin-stimulated. This suggests that H-3-DG uptake by both cell types is mediated by members of the...
Cardiorespiratory fitness is negatively associated with metabolic risk factors independently of the adherence to a healthy dietary pattern - Moreira, C.; Santos, R.; Moreira, Pedro; Lobelo, F.; Ruiz, J.; Vale, S.; Santos, P.; Abreu, S.; Mota, J.
Background and aim: Cardiorespiratory fitness (CRF) and diet have been involved as significant factors towards the prevention of cardio-metabolic diseases. This study aimed to assess the impact of the combined associations of CRF and adherence to the Southern European Atlantic Diet (SEADiet) on the clustering of metabolic risk factors in adolescents.
Methods and Results: A cross-sectional school-based study was conducted on 468 adolescents aged 15-18, from the Azorean Islands, Portugal. We measured fasting glucose, insulin, total cholesterol (TC), HDL-cholesterol, triglycerides, systolic blood pressure, waits circumference and height. HOMA, TC/HDL-C ratio and waist-to-height ratio were calculated. For each of these variables,...