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FADEUP - Artigo em Revista Internacional
Mostrando recursos 1 - 20 de 147
Food Handler´s Perception of Fresh Cut Products - Barros, Manuela; Rocha, Ada
The use of Fresh Cut Products (FCP) allows food service units to offer a greater variety of menus, reducing costs and
minimizing cross contamination. Few food units in Portugal are adopting FCP, since these products are viewed as being more
expensive and are regarded as less natural and easily perishable.The present study aims to identify and analyze the level of awareness of
food service operators in respect to FCP. Approximately 90% of food handlers referred the use of FCP at their workplace, pointing as
the main advantages for using them the less time spent on preparation (58.6%), the variety (55.9%) and the convenience (55.9%).
The Impact of an Intervention Taught by Trained Teachers on Childhood Overweight - Rosário, Rafaela; Oliveira, Bruno; Araújo, Ana; Lopes, Óscar; Padrão, Patrícia; Moreira, André; Teixeira, Vitor; Barros, Renata; Pereira, Beatriz; Moreira, Pedro
The purpose of this study was to assess the effects of a six-months' nutrition program, delivered and taught by classroom teachers with in-service nutrition training, on the prevention of overweight and obesity among children in grades 1 to 4. In this randomized trial, four hundred and sixty four children from seven elementary schools were allocated to a nutrition educational program delivered by their own teachers. Intervened teachers had 12 sessions of three hours each with the researchers throughout six months, according to the topics nutrition and healthy eating, the importance of drinking water and healthy cooking activities. After each session,...
The impact of an intervention taught by trained teachers on childhood fruit and vegetable intake: a randomized trial - Rosário, Rafaela; Araújo, Ana; Oliveira, Bruno; Padrão, Patrícia; Lopes, Óscar; Teixeira, Vitor Hugo; Moreira, André; Barros, Renata; Pereira, Beatriz; Moreira, Pedro
Our study aimed to assess the impact of a six-months nutrition program, taught by trained teachers, on fruit and vegetable consumption among children in grades 1 to 4. Four hundred and sixty-four children (239 female), 6 to 12 years old, from seven elementary schools were assigned to this randomized trial. Teachers were trained by researchers over six months, according to the following topics: nutrition, healthy eating, and strategies to increase physical activity. After each session, teachers were encouraged to develop activities in the classroom on the topics learned. Children's sociodemographic, anthropometric, dietary, and physical activity data were assessed at baseline...
A importância dos fatores estruturais na garantia da segurança alimentar na produção de refeições para escolares - Correia, Maria João; Rocha, Ada
The design of school canteens shall be subject to detailed studies regarding its functional and operational activity. It must consider several factors such as legal aspects, production technical requirements, architectural and working conditions as well as economic aspects in order to guarantee the correct and safe supply of the meals.
In addition to literature review some technical visits were done to allow the on-site checking of the fulfilment of the several factors considered.
Several structural nonconformities were identified that affect compliance with the good manufacturing practices. Most of them could have been avoided if, at the design stage of the project, these...
Expression of apoptosis-regulating genes in the rat prostate following botulinum toxin type A injection - Gorgal, T.; Charrua, Ana; Silva, J.F.; Avelino, A.; Dinis, P.; Cruz, F.
Background: Onabotulinumtoxin A (OnabotA) injection has been investigated as a novel treatment for benign prostatic enlargement caused by benign prostatic hyperplasia. An OnabotA - induced volume reduction caused by sympathetic fibers impairment has been proposed as a potential mechanism of action. Our aim was to investigate the expression of apoptosis-regulating proteins in the rat prostate following OnabotA intraprostatic injection.
Methods: Adult Wistar rats were injected in the ventral lobes of the prostate with 10 U of OnabotA or saline. A set of OnabotA-injected animals was further treated with 0.5 mg/kg of phenylephrine (PHE) subcutaneously daily. All animals were sacrificed after...
Superoxide Dismutase Detection in Human Cumulus Oophorus Cells - Matos L; Stevenson D; Gomes F; Silva-Carvalho JL; Almeida H
A key factor limiting the success of Assisted Reproduction Techniques (ART) is oocyte and embryo quality, usually assessed by their morphologic appearance. Due to the subjectivity and inaccuracy of such criteria, other predictors of oocyte and embryo quality would be welcome. Cumulus oophorus (c.o.) cells are eligible as oocyte quality predictors, due to their direct contact with it. In addition, ART success was also related to oxidative stress, whose effects in the porcine and bovine oocytes may be prevented by the presence of c.o. cells. Yet, it is unknown if a similar effect exists in humans due to the scarcity...
Method Validation for Cafestol and Kahweol Quantification in Coffee Brews by HPLC-DAD - José A. Silva; Nuno Borges; Alejandro Santos; Arminda Alves
Cafestol and kahweol are two diterpenes exclusively
found in coffee. Negative effects on health, like the
increase in total lipid blood levels as well as positive effects
like carcinogenic protective and antioxidant activity, have
been reported for these two compounds. A simple and
expeditious method for the quantification of total cafestol
and kahweol levels in coffee brews by high-performance
liquid chromatography with diode array detection was
developed and validated. Esterified diterpenes were hydrolysed and resulting diterpene alcohols extracted with diethylether. For cafestol limit of detection was 0.01 mg L−1 and limit of quatification was 0.04 mg L−1. Coefficient of variation for cafestol ranged between 0.2 and 2.8 %...
Quality of Life Experienced by Adolescents and Young Adults with Congenital Heart Disease - Teixeira, Flávio; Coelho, Rosália; Proença, Cidália; Silva, Ana; Vieira, Daniela; Vaz, Cláudia; Moura, Cláudia; Viana, Victor; Areias, José; Areias, Maria
This study aimed to assess the quality of life (QOL) experienced by adolescents and young adults with
congenital heart disease (CHD) and to determine which
factors negatively affect adjustment and which factors
increase resilience. The participants in the study were 74
patients with CHD (41 males and 33 females) ranging in age
from 12 to 26 years (mean age, 18.76 ± 3.86 years).
Demographic information and a complete clinical history
were obtained. The participants were interviewed regarding
topics such as social support, family educational style, selfimage, and physical limitations. They responded to questions in a standardized psychiatric interview (SADS-L) and completed a self-report questionnaire (WHOQOL-BREF)
for assessment of QOL....
Left intraventricular diastolic and systolic pressure gradients - Guerra, Miguel; Sampaio, Francisco; Brás-Silva, Carmen; Leite-Moreira, Adelino
To describe left ventricular (LV) function comprehensively, it is crucial to characterize precisely transmitral, intraventricular and transaortic pressure-flow relations. The site of measurement is important; as the measurement location is moved from the mitral valve toward the apex and the outflow tract, important regional pressure differences are recorded inside the LV. These intraventricular pressure gradients (IVPGs) play an important role in ventricular filling in the normal heart and may be abolished by systolic or diastolic dysfunction. Despite their apparent importance in ventricular filling and diastolic function, IVPGs have never been utilized in clinical cardiology, due to the complexity of their...
Modulation of myocardial stiffness by beta-adrenergic stimulation - its role in normal and failing heart - Falcão-Pires, I.; Sousa, Adelino Fontes; Conceiçao, L. Lopes; Brás-Silva, Carmen; Leite-Moreira, Adelino
The acute effects of beta-adrenergic stimulation on myocardial stiffness were evaluated. New-Zealand white rabbits were treated with saline (control group) or doxorubicin to induce heart failure (HF) (DOXO-HF group). Effects of isoprenaline (10(-10)-10(-5) M), a non-selective beta-adrenergic agonist, were tested in papillary muscles from both groups. In the control group, the effects of isoprenaline were also evaluated in the presence of a damaged endocardial endothelium, atenolol (beta(1)-adrenoceptor antagonist), ICI-118551 (beta(2)-adrenoceptor antagonist), KT-5720 (PKA inhibitor), L-NNA (NO-synthase inhibitor), or indomethacin (cyclooxygenase inhibitor). Passive length-tension relations were constructed before and after adding isoprenaline (10(-5) M). In the control group, isoprenaline increased resting...
Quality of life of patients with congenital heart diseases - Silva, Ana; Vaz, Cláudia; Areias, Maria; Vieira, Daniela; Proenca, Cidália; Viana, Victor; Moura, Cláudia; Areias, José
Objectives: To assess the perception of the quality of life of adolescents and young adults with congenital heart disease and to examine the variables that have a negative impact on it and that add a resilience effect. Methods: A total of 22 male and 18 female patients, aged 12–26 years, of whom 27 were admitted to surgery and 13 were not, participated in this study. All patients had complete medical records and were interviewed once; demographic and clinical data were collected, and patients filled a questionnaire on quality of life, the WHOQOL-BREF, and underwent an interview on social support, educational...
Copper ability to induce premature senescence in human fibroblasts - Matos, Liliana; Gouveia, Alexandra; Almeida, Henrique
Human diploid fibroblasts (HDFs) exposed to subcytotoxic concentrations of oxidative or stressful agents, such as hydrogen peroxide, tert-butylhydroperoxide, or ethanol, undergo stress-induced premature senescence (SIPS). This condition is characterized by the appearance of replicative senescence biomarkers such as irreversible growth arrest, increase in senescence-associated β-galactosidase (SA β-gal) activity, altered cell morphology, and overexpression of several senescence-associated genes. Copper is an essential trace element known to accumulate with ageing and to be involved in the pathogenesis of some age-related disorders. Past studies using either yeast or human cellular models of ageing provided evidence in favor of the role of intracellular copper...
Dissecting mitosis by RNAi in Drosophila tissue culture cells
interference (RNAi) in cultured cells of Drosophila melanogaster. This procedure is particularly useful for the analysis of genes for which
genetic mutations are not available or for the dissection of complicated phenotypes derived from the analysis of such mutants. With the
advent of whole genome sequencing it is expected that RNAi-based screenings will be one method of choice for the identification and
study of novel genes involved in particular cellular processes. In this paper we focused particularly on the procedures for the proper
phenotypic analysis of cells after RNAi-mediated depletion of proteins required for mitosis, the process by which the genetic
information is segregated equally...