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Repositorio Institucional de la Universidad de Cordoba :: Spain (51.670 recursos)

El repositorio recoge todo tipo de materiales digitales: artículos de revistas, comunicaciones a congresos, tesis doctorales, documentos de trabajo, materiales docentes y objetos de aprendizaje, así como los productos digitales del patrimonio bibliográfico de la Universidad de Córdoba.

DGen-Artículos, capítulos...

Mostrando recursos 1 - 20 de 469

  1. Análise comparativa da qualidade de panificaçao e rendimento de graos entre tritórdeo hexaplóide e outros cereais de inverno

    Barth Pinto, Ronald José; Scapim, Carlos Alberto; Álvarez, Juan Bautista; Martín Martín, L.M.
    A duplicação cromossômica dos híbridos entre Hordeum chilense e Triticum turgidum conv. durum originou o tritórdeo hexaplóide (X Tritordeum Ascherson et Graebner), um novo cereal cujas características estão sendo avaliadas. Com o objetivo de obter maiores informações sobre o potencial do tritórdeo para a qualidade de panificação e componentes do rendimento de grãos, foi instalado em 1996 em Córdoba, Espanha, um experimento de campo com três repetições. O desempenho de três linhagens de tritórdeo foi comparado com o rendimento de duas variedades comerciais de trigo, duas de trigo duro e quatro de triticale. O conjunto de resultados indicou perspectivas do tritórdeo para o desenvolvimento de variedades altamente...

  2. Allelic Heterogeneity at the Equine KIT Locus in Dominant White (W) Horses

    Haase, Bianca; Brooks, Samantha; Schlumbaum, Angela; Azor, P.J.; Bailey, Ernest; Alaeddine, Ferial; Mevissen, Meike; Burger, Dominik; Poncet, Pierre-André; Rieder, Stefan; Leeb, Tosso
    White coat color has been a highly valued trait in horses for at least 2,000 years. Dominant white (W) is one of several known depigmentation phenotypes in horses. It shows considerable phenotypic variation, ranging from ;50% depigmented areas up to a completely white coat. In the horse, the four depigmentation phenotypes roan, sabino, tobiano, and dominant white were independently mapped to a chromosomal region on ECA 3 harboring the KIT gene. KIT plays an important role in melanoblast survival during embryonic development. We determined the sequence and genomic organization of the ;82 kb equine KIT gene. A mutation analysis of all 21 KIT exons in...

  3. Variation of High Molecular Weight Glutenin Subunits in Two Neglected Tetraploid Wheat Subspecies

    Caballero García, Leonor; Martín Martín, L.M.; Álvarez Cabello, J.B.
    The genetic diversity of 140 accessions of Triticum turgidum ssp. carthlicum Nevski em. A. Löve & D. Löve and 159 accessions of T. turgidum ssp. polonicum L. em. Thell. was evaluated by the analysis of HMW glutenin subunits. Seven allelic variants were found among the carthlicum accessions: three at the Glu-A1 locus (two of them were novel alleles) and four at the Glu-B1 locus (one of them novel). More variability was found among the polonicum accessions with 16 allelic variants: six at the Glu-A1 locus (three of them novel), and ten at the Glu-B1 locus (five of them novel). Totally, ten new alleles were found,...

  4. Biological pathway analysis by ArrayUnlock and Ingenuity Pathway Analysis

    Jiménez-Marín, Ángeles; Collado-Romero, Melania; Ramírez-Boo, M.; Arce Jiménez, Cristina; Garrido, Juan J.
    Background: Once a list of differentially expressed genes has been identified from a microarray experiment, a subsequent post-analysis task is required in order to find the main biological processes associated to the experimental system. This paper describes two pathways analysis tools, ArrayUnlock and Ingenuity Pathways Analysis (IPA) to deal with the post-analyses of microarray data, in the context of the EADGENE and SABRE post-analysis workshop. Dataset employed in this study proceeded from an experimental chicken infection performed to study the host reactions after a homologous or heterologous secondary challenge with two species of Eimeria. Results: Analysis of the same microarray data source employing both commercial pathway analysis tools...

  5. Gene expression pattern in swine neutrophils after lipopolysaccharide exposure: a time course comparison

    Sanz-Santos, Gema; Jiménez-Marín, Ángeles; Bautista, Rocío; Fernández, Noé; Claros, Gonzalo M.; Garrido, Juan J.
    Background: Experimental exposure of swine neutrophils to bacterial lipopolysaccharide (LPS) represents a model to study the innate immune response during bacterial infection. Neutrophils can effectively limit the infection by secreting lipid mediators, antimicrobial molecules and a combination of reactive oxygen species (ROS) without new synthesis of proteins. However, it is known that neutrophils can modify the gene expression after LPS exposure. We performed microarray gene expression analysis in order to elucidate the less known transcriptional response of neutrophils during infection. Methods: Blood samples were collected from four healthy Iberian pigs and neutrophils were isolated and incubated during 6, 9 and 18 hrs in presence or absence of...

  6. Genetic characterization of Appenninica sheep breed by microsatellites

    Lasagna, Emiliano; Landi, Vincenzo; Bianchi, Matteo; Martínez Martínez, Amparo; Sarti, Francesca Maria
    The conservation of genetic variability is one of the main objectives in the field of genetics applied to domestic livestock. A strong input in that direction was made by molecular biology techniques. Among molecular markers nowadays available, microsatellites are particularly appreciated and widely utilized for the study of animal genome. In this study, a genetic characterization of Appenninica sheep breed was carried out with thirty microsatellite markers; the genetic relationships between Appenninica and three Italian merinos-derived breeds were also investigated. The results show a good genetic variability of Appenninica and all the other studied breeds. At the same time, the genetic identity of each breed is confirmed....

  7. Genetic diversity and relationships among Italian Merino derived breeds assessed by microsatellites

    Ceccobelli, Simone; Lasagna, Emiliano; Landi, Vincenzo; Martínez Martínez, Amparo; Sarti, Francesca Maria
    The conservation of genetic variability is one of the main objectives in the field of genetics applied to domestic livestock. Among molecular markers, microsatellites are particularly appreciated and widely utilized for the study of animal genome. In this study a genetic characterization of three Italian Merino derived sheep breeds was carried out by 30 microsatellites markers; the genetic relationships between these breeds and the Spanish ancestors were also investigated. All the microsatellites examined resulted polymorphic and a total of 375 alleles were detected. FIS values of the three Italian Merinos demonstrate a low level of inbreeding. The results show a good genetic variability of all the studied...

  8. Genetic structure, relationships and admixture with wild relatives in native pig breeds from Iberia and its islands

    Gama, Luis L.T.; Martínez Martínez, Amparo; Carolino, Inés; Landi, Vincenzo; Delgado-Bermejo, J.V.; Vicente, Antonio A.; Vega-Pla, J.L.; Cortés, Oscar; Sousa, Conceiçao O; BIOPIG Consortium
    Background: Native pig breeds in the Iberian Peninsula are broadly classified as belonging to either the Celtic or the Mediterranean breed groups, but there are other local populations that do not fit into any of these groups. Most of the native pig breeds in Iberia are in danger of extinction, and the assessment of their genetic diversity and population structure, relationships and possible admixture between breeds, and the appraisal of conservation alternatives are crucial to adopt appropriate management strategies. Methods: A panel of 24 microsatellite markers was used to genotype 844 animals representing the 17 most important native swine breeds and wild populations existing in Portugal...

  9. Quantitative analysis of the immune response upon Salmonella typhimurium infection along the porcine intestinal gut

    Collado-Romero, Melania; Arce Jiménez, Cristina; Ramírez-Boo, M.; Carvajal, Ana; Garrido, Juan J.
    Salmonella enterica serovar Typhimurium causes enteric disease and compromises food safety. In pigs, the molecular response of the intestine to S. typhimurium has been traditionally characterized by in vitro models that do not reflect the actual immunological competence of the intestinal mucosa. In this work, we performed an oral S. typhimurium infection study to obtain insight into the in vitro response in three different sections (jejunum, ileum and colon) of the porcine intestine. For this, samples from onemonth- old infected piglets were collected during a time course comprising 1, 2, and 6 days post inoculation to evaluate the intestinal response by quantifying the mRNA expression of...

  10. Genetic resources of Spanish hulled wheats

    Caballero García, Leonor; Martín Martín, L.M.; Álvarez Cabello, J.B.
    Emmer (Triticum turgidum ssp. dicoccum Schrank em Thell, syn T. dicoccon Schrank) and spelt (T. aestivum ssp. spelta L. em Thell.) are two hulled wheats that are still grown in some Spanish regions, mainly in Asturias (North of Spain), where are indistinctly named as escanda. One recent collecting mission carried out in this Spanish zone has showed the scarce presence of emmer (4 populations), together with the presence of foreigner spelt that could change the genetic patrimony of the escanda, because of present agronomic characteristics more adapted to the present requirements than the autochthon spelt lines. This supposes one clear danger to the maintaining of these...

  11. ROS1 5-methylcytosine DNA glycosylase is a slow-turnover catalyst that initiates DNA demethylation in a distributive fashion

    Ponferrada-Marín, María Isabel; Roldán-Arjona, Teresa; Rodríguez Ariza, Rafael
    Arabidopsis ROS1 belongs to a family of plant 5-methycytosine DNA glycosylases that initiate DNA demethylation through base excision. ROS1 displays the remarkable capacity to excise 5-meC, and to a lesser extent T, while retaining the ability to discriminate effectively against C and U. We found that replacement of the C5-methyl group by halogen substituents greatly decreased excision of the target base. Furthermore, 5-meC was excised more efficiently from mismatches, whereas excision of T only occurred when mispaired with G. These results suggest that ROS1 specificity arises by a combination of selective recognition at the active site and thermodynamic stability of the target base. We also found that ROS1 is a low-turnover catalyst because it binds...

  12. A discontinuous DNA glycosylase domain in a family of enzymes that excise 5-methylcytosine

    Ponferrada-Marín, María Isabel; Parrilla-Doblas, Jara; Roldán-Arjona, Teresa; Rodríguez Ariza, Rafael
    DNA cytosine methylation (5-meC) is a widespread epigenetic mark associated to gene silencing. In plants, DEMETER-LIKE (DML) proteins typified by Arabidopsis REPRESSOR OF SILENCING 1 (ROS1) initiate active DNA demethylation by catalyzing 5-meC excision. DML proteins belong to the HhH-GPD superfamily, the largest and most functionally diverse group of DNA glycosylases, but the molecular properties that underlie their capacity to specifically recognize and excise 5-meC are largely unknown. We have found that sequence similarity to HhH-GPD enzymes in DML proteins is actually distributed over two non-contiguous segments connected by a predicted disordered region. We used homology-based modeling to locate candidate residues important for ROS1 function in both segments, and tested our predictions by site-specific mutagenesis. We found...

  13. Molecular Cloning, Characterization, Expression Analysis and Chromosomal Localization of the Gene Coding for the Porcine αIIb Subunit of the αIIbβ3 Integrin Platelet Receptor

    Esteso, Gloria; Jiménez-Marín, Ángeles; Sanz-Santos, Gema; Garrido, Juan J.; Barbancho Medina, Manuel

  14. Immunohistochemical analysis of ß3 integrin (CD61): expression in pig tissues and human tumors

    Moreno, Ángela; Lucena, Concepción; López, A.; Garrido, Juan J.; Pérez de la Lastra, J.M.; Llanes Ruiz, Diego
    CD61 is a membrane glycoprotein that associates with CD41 (aIIb) to form the heterodimeric complex gpIIb/IIIa (CD41/CD61), predominantly expressed in platelets and megakariocytes. CD61 or ß3 integrin also associates with av (CD51) to form the vitronectin receptor, which is expressed in many tissues. We have used a monoclonal antibody against the porcine gpIIIa or CD61 (JM2E5) to study the distribution of this molecule in different normal pig tissues. As in humans, CD61 was broadly expressed in all tissues examined. In the kidney, strong expression of CD61 was observed in epithelial cells from renal tubules. In the testis, CD61 expression was detected in the Leydig cells. However, in liver, CD61 was weak or not...

  15. Short communication. In vitro oocyte maturation and fertilization rates in the Spanish Lidia bovine breed

    Hidalgo, M.; Dorado, J.; Anter, J.; Demyda-Peyrás, S.; Género, Enrique Rubén; Moreno-Millán, M.
    The Lidia bovine breed is the most successful cattle breed on the Iberian Peninsula, also considered a hallmark of Spanish tradition and image around the world. The aims of the study were to characterize the oocyte recovery rates and to evaluate the effect of two standard in vitro maturation protocols on oocyte maturation (cumulus expansion and nuclear maturation) and fertilization rates after in vitro fertilization in this breed. For this purpose, 261 ovaries from Lidia cows were processed obtaining 1,125 viable cumulus oocyte complexes (COCs). The oocyte recovery rate obtained (4.31 viable COCs per ovary) was lower than those described previously in other studied breeds....

  16. Resultados preliminares del efecto de la temperatura y humedad relativa sobre la producción de leche y sus componentes en cabras de raza Payoya

    Romero, F.; Molina Alcalá, Antonio; González, O.; Clemente López, Ignacio; Arrebola, F.; Menéndez-Buxadera, A.
    Un total de 81625 registros mensuales de 8380 hembras de la raza Payoya paridas en 18 ganaderías entre 2004 y 2007 fueron estudiados aplicando diferentes modelos lineales, con el objetivo de estimar los posibles efectos de un índice que combina Temperatura y Humedad Relativa (THI), sobre las variables de producción de leche y sus características físico-químicas. Los primeros resultados indicaron que existe una zona de termoneutralidad (THI entre 13 y 22) en la cual no se han evidenciado claramente los efectos del estrés térmico. Para valores de THI>22 se creó una función fT para estimar los componentes genéticos generales y los debidos al estrés térmico. Las...

  17. Caracterización fenotípica y genética de caracteres morfo-funcionales en el caballo de pura raza menorquina: desarrollo de nuevas estrategias para su aplicación en el programa de mejora de la raza

    Solé Berga, Marina
    El Caballo de Pura Raza Menorquina (PRMe) es una raza equina autóctona, catalogada en Peligro de Extinción según el Catálogo oficial de Razas de Ganado de España (Orden AAA/251/2012 de 9 de Febrero, por la que se modifica el Anexo I del RD 2129/2008, de 26 de diciembre. BOE-A-2012-2254). Desde 1988 está reconocida como raza pura, explotándose principalmente en la Isla de Menorca (Islas Baleares -España-). Su Programa de Mejora Genética se aprobó mediante la resolución del 10 de marzo de 2010 (BOE-A-2010-5478). El Caballo PRMe es un animal adecuado para la equitación, participando en competiciones deportivas de Doma Clásica y Doma Menorquina, y para la silla, siendo...

  18. Consumo de agua de ovinos alimentados con diferentes niveles de nopal (Opuntia Ficus Indica) en Brasil

    Costa, R.G.; Hernández, T.I.; Medeiros, G.R.; Medeiros, A.N.; Azevedo, P.S.; Pinto, T.F.; Delgado-Bermejo, J.V.
    En 45 ovinos machos Santa Inés (peso vivo medio de 27,50 ± 0, 48 kg) se evaluó el consumo de agua, al consumir raciones con niveles crecientes (0, 25, 50, 75, 100%) de nopal (Opuntia fícus indica Mill) en sustitución al maíz molido. El diseño fue en bloques al azar con nueve repeticiones. El consumo de materia seca respondió de modo cuadrático, perjudicando el peso final, que disminuyó, con el aumento del nopal en la dieta. Aumentó el consumo de materia natural disminuyendo el de agua, el consumo total de agua aumentó en el tratamiento con mayor cantidad de nopal. La relación de consumos: agua voluntaria/ materia seca, disminuyó al aumentar el nopal....

  19. Análisis preliminar de parámetros genéticos para el carácrter de prolificidad en la oveja canaria de pelo

    Delgado-Bermejo, J.V.; León Jurado, J.M.; Bermejo, L.A.; López-Gallego, F.; Camacho, A.; Santolaria, J.
    En este trabajo se presentan los valores de los parámetros genéticos para prolificidad de la oveja Canaria de Pelo, obtenidos a partir de la utilización de un modelo animal con observaciones repetidas sobre la información generada en los núcleos de control de rendimiento cárnico de la raza. El modelo de análisis genético incluyó como efectos fijos: ganadería, año, época y número de parto; y como efectos aleatorios, el genotipo aditivo del animal y el efecto ambiental permanente. Se utilizaron, como base de información, un total de 7657 partos registrados en 2889 animales de 5 ganaderías entre los años 1996 y 2008, lo que supuso un promedio de 2,65 partos registrados por animal, suficiente para...

  20. Bioinformatics tools for inferring immune-relatedfunctions from proteomic data

    Sanz-Santos, Gema; Jiménez-Marín, Ángeles; Moreno, Ángela; Garrido, Juan J.
    Comunicaciones a congresos

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