Recursos de colección

Repositorio Institucional de la Universidad de Cordoba :: Spain (56.505 recursos)

El repositorio recoge todo tipo de materiales digitales: artículos de revistas, comunicaciones a congresos, tesis doctorales, documentos de trabajo, materiales docentes y objetos de aprendizaje, así como los productos digitales del patrimonio bibliográfico de la Universidad de Córdoba.

DGen-Artículos, capítulos...

Mostrando recursos 1 - 20 de 157

  1. Genetic structure, relationships and admixture with wild relatives in native pig breeds from Iberia and its islands

    Gama, Luis L.T.; Martínez Martínez, Amparo; Carolino, Inés; Landi, Vincenzo; Vicente, Antonio A.; Vega-Pla, J.L.; Cortés, Oscar; Sousa, Conceiçao O; BIOPIG Consortium; Delgado-Bermejo, J.V.
    Background: Native pig breeds in the Iberian Peninsula are broadly classified as belonging to either the Celtic or the Mediterranean breed groups, but there are other local populations that do not fit into any of these groups. Most of the native pig breeds in Iberia are in danger of extinction, and the assessment of their genetic diversity and population structure, relationships and possible admixture between breeds, and the appraisal of conservation alternatives are crucial to adopt appropriate management strategies. Methods: A panel of 24 microsatellite markers was used to genotype 844 animals representing the 17 most important native swine breeds and wild populations existing in Portugal...

  2. ROS1 5-methylcytosine DNA glycosylase is a slow-turnover catalyst that initiates DNA demethylation in a distributive fashion

    Roldán-Arjona, Teresa; Rodríguez Ariza, Rafael; Ponferrada-Marín, María Isabel
    Arabidopsis ROS1 belongs to a family of plant 5-methycytosine DNA glycosylases that initiate DNA demethylation through base excision. ROS1 displays the remarkable capacity to excise 5-meC, and to a lesser extent T, while retaining the ability to discriminate effectively against C and U. We found that replacement of the C5-methyl group by halogen substituents greatly decreased excision of the target base. Furthermore, 5-meC was excised more efficiently from mismatches, whereas excision of T only occurred when mispaired with G. These results suggest that ROS1 specificity arises by a combination of selective recognition at the active site and thermodynamic stability of the target base. We also found that ROS1 is a low-turnover catalyst because it binds...

  3. A discontinuous DNA glycosylase domain in a family of enzymes that excise 5-methylcytosine

    Parrilla-Doblas, Jara; Roldán-Arjona, Teresa; Rodríguez Ariza, Rafael; Ponferrada-Marín, María Isabel
    DNA cytosine methylation (5-meC) is a widespread epigenetic mark associated to gene silencing. In plants, DEMETER-LIKE (DML) proteins typified by Arabidopsis REPRESSOR OF SILENCING 1 (ROS1) initiate active DNA demethylation by catalyzing 5-meC excision. DML proteins belong to the HhH-GPD superfamily, the largest and most functionally diverse group of DNA glycosylases, but the molecular properties that underlie their capacity to specifically recognize and excise 5-meC are largely unknown. We have found that sequence similarity to HhH-GPD enzymes in DML proteins is actually distributed over two non-contiguous segments connected by a predicted disordered region. We used homology-based modeling to locate candidate residues important for ROS1 function in both segments, and tested our predictions by site-specific mutagenesis. We found...

  4. Immunohistochemical analysis of ß3 integrin (CD61): expression in pig tissues and human tumors

    Moreno, Ángela; Lucena, Concepción; López, A.; Garrido, Juan J.; Pérez de la Lastra, J.M.; Llanes Ruiz, Diego
    CD61 is a membrane glycoprotein that associates with CD41 (aIIb) to form the heterodimeric complex gpIIb/IIIa (CD41/CD61), predominantly expressed in platelets and megakariocytes. CD61 or ß3 integrin also associates with av (CD51) to form the vitronectin receptor, which is expressed in many tissues. We have used a monoclonal antibody against the porcine gpIIIa or CD61 (JM2E5) to study the distribution of this molecule in different normal pig tissues. As in humans, CD61 was broadly expressed in all tissues examined. In the kidney, strong expression of CD61 was observed in epithelial cells from renal tubules. In the testis, CD61 expression was detected in the Leydig cells. However, in liver, CD61 was weak or not...

  5. Consumo de agua de ovinos alimentados con diferentes niveles de nopal (Opuntia Ficus Indica) en Brasil

    Costa, R.G.; Hernández, T.I.; Medeiros, G.R.; Medeiros, A.N.; Azevedo, P.S.; Pinto, T.F.; Delgado-Bermejo, J.V.
    En 45 ovinos machos Santa Inés (peso vivo medio de 27,50 ± 0, 48 kg) se evaluó el consumo de agua, al consumir raciones con niveles crecientes (0, 25, 50, 75, 100%) de nopal (Opuntia fícus indica Mill) en sustitución al maíz molido. El diseño fue en bloques al azar con nueve repeticiones. El consumo de materia seca respondió de modo cuadrático, perjudicando el peso final, que disminuyó, con el aumento del nopal en la dieta. Aumentó el consumo de materia natural disminuyendo el de agua, el consumo total de agua aumentó en el tratamiento con mayor cantidad de nopal. La relación de consumos: agua voluntaria/ materia seca, disminuyó al aumentar el nopal....

  6. Análisis preliminar de parámetros genéticos para el carácrter de prolificidad en la oveja canaria de pelo

    Bermejo, L.A.; López-Gallego, F.; Camacho, A.; Santolaria, J.; Delgado-Bermejo, J.V.
    En este trabajo se presentan los valores de los parámetros genéticos para prolificidad de la oveja Canaria de Pelo, obtenidos a partir de la utilización de un modelo animal con observaciones repetidas sobre la información generada en los núcleos de control de rendimiento cárnico de la raza. El modelo de análisis genético incluyó como efectos fijos: ganadería, año, época y número de parto; y como efectos aleatorios, el genotipo aditivo del animal y el efecto ambiental permanente. Se utilizaron, como base de información, un total de 7657 partos registrados en 2889 animales de 5 ganaderías entre los años 1996 y 2008, lo que supuso un promedio de 2,65 partos registrados por animal, suficiente para...

  7. DNA testing for parentage verification in a conservation nucleus of Pantaneiro horse

    Sereno, Fabiana Tavares Pires de Souza; Sereno, José Robson Bezerra; Vega-Pla, J.L.; Delgado-Bermejo, J.V.
    We investigated the genealogy of the in situ conservation nucleus of the Pantaneiro horse using DNA microsatellites by evaluating 101 horses, the group consisting of 71 adult horses (3 stallions, 40 male and 31 mares) and 27 foals (14 colts and 13 fillies). Genomic DNA was extracted from hair roots and genotyped using 12 microsatellite markers (AHT4, AHT5, ASB2, ASB17, ASB23, HMS3 HMS6, HMS7, HTG4, HTG10, LEX33 and VHL20). The number of alleles per locus varied from 6 to 13, with a mean of 7.8 and the expected heterozygosity ranged from 0.544 to 0.734 (mean 0.644). The VLH20, ASB2, HTG10, ASB23 markers had a high...

  8. Homologous Recombination Is Stimulated by a Decrease in dUTPase in Arabidopsis

    Dubois, Emeline; Massot, Sophie; Siaud, Nicolas; Gakière, Bertrand; Domemichini, Séverine; Guérard, Florence; Roldán-Arjona, Teresa; Doutriaux, Mrie-Pascale; Córdoba-Cañero, Dolores
    Deoxyuridine triphosphatase (dUTPase) enzyme is an essential enzyme that protects DNA against uracil incorporation. No organism can tolerate the absence of this activity. In this article, we show that dUTPase function is conserved between E. coli (Escherichia coli), yeast (Saccharomyces cerevisiae) and Arabidopsis (Arabidopsis thaliana) and that it is essential in Arabidopsis as in both micro-organisms. Using a RNA interference strategy, plant lines were generated with a diminished dUTPase activity as compared to the wild-type. These plants are sensitive to 5-fluoro-uracil. As an indication of DNA damage, inactivation of dUTPase results in the induction of AtRAD51 and AtPARP2, which are involved in DNA repair. Nevertheless, RNAi/DUT1...

  9. Bases moleculares de la desmetilación de DNA en Arabidopsis thaliana

    Parrilla-Doblas, Jara; Roldán-Arjona, Teresa; Rodríguez Ariza, Rafael; Ponferrada-Marín, María Isabel
    Resumen de la comunicación presentada al XX Congreso de la Sociedad Española de Mutagénesis Ambiental – Córdoba 2012

  10. Genetic Footprints of Iberian Cattle in America 500 Years after the Arrival of Columbus

    Martínez Martínez, Amparo; Gama, Luis L.T.; Cañón, Javier; Ginja, Catarina; Dunner, Susana; Landi, Vincenzo; Martín-Burriel, I.; Penedo, M. Cecilia T.; Rodellar, Clementina; Vega-Pla, J.L.; Acosta, Atzel; Álvarez, Luz Ángela; Camacho Vallejo, M.E.; Cortés, Oscar; Marques, J.R.; Martínez, Roberto; Martínez, Ruben D.; Melucci, Lilia; Martínez-Velázquez, Guillermo; Muñoz, Jaime E.; Postiglioni, A.; Quiroz, J.; Sponenberg, Philip; Uffo, Odalys; Villalobos, Axel; Zaragoza, P.; Zambrano, Delsito; Delgado-Bermejo, J.V.
    Background: American Creole cattle presumably descend from animals imported from the Iberian Peninsula during the period of colonization and settlement, through different migration routes, andmay have also suffered the influence of cattle directly imported from Africa. The introduction of European cattle, which began in the 18th century, and later of Zebu from India, has threatened the survival of Creole populations, some of which have nearly disappeared or were admixed with exotic breeds. Assessment of the genetic status of Creole cattle is essential for the establishment of conservation programs of these historical resources. Methodology/Principal Findings: We sampled 27 Creole populations, 39 Iberian, 9 European and 6...

  11. Programa de conservación de la raza blanca andaluza: primeros resultados y perspectivas

    Cabello Robles, A.; Romero Obrero, A.; Camacho Vallejo, M.E.; Delgado-Bermejo, J.V.
    Resumen de la comunicación presentada al III Congreso Ibérico sobre Recursos Genéticos Animales

  12. Diez mil años de agricultura

    Cubero Salmerón, José Ignacio
    Fondo procedente de la donación Marcos-Vicent

  13. Evaluación de la longevidad productiva en ovejas segureñas de núcleo de control

    Rodríguez, J.V.; Benavente, M.; Puntas, J.; Barba Capote, C.J.; Delgado-Bermejo, J.V.
    Resumen de la comunicación presentada al III Congreso Ibérico sobre Recursos Genéticos Animales

  14. Propuesta de clasificación del cerdo ibérico en base a marcadores genéticos moleculares

    Martínez Martínez, Amparo; Vega-Pla, J.L.; Delgado-Bermejo, J.V.
    Resumen de la comunicación presentada al III Congreso Ibérico sobre Recursos Genéticos Animales

  15. Caracterización genética del caballo pantaneiro brasileño a través de microsatélites de ADN

    Sereno, Fabiana Tavares Pires de Souza; Sereno, José Robson Bezerra; Rodríguez-Gallardo, P.P.; Vega-Pla, J.L.; Delgado-Bermejo, J.V.
    Resumen de la comunicación presentada al III Congreso Ibérico sobre Recursos Genéticos Animales

  16. El cerdo negro de Los Pedroches. Perfil genético mediante marcadores moleculares

    Martínez Martínez, Amparo; Vega-Pla, J.L.; Escribano Cabrera, F.; Delgado-Bermejo, J.V.
    Resumen de la comunicación presentada al III Congreso Ibérico sobre Recursos Genéticos Animales

  17. La prolificidad en la oveja segureña

    Rodríguez, J.V.; Benavente, M.; Puntas, J.; Barba Capote, C.J.; Delgado-Bermejo, J.V.
    Resumen de la comunicación presentada al III Congreso Ibérico sobre Recursos Genéticos Animales

  18. Producción de semen y desarrollo testicular en los machos de la Agrupación Caprina Canaria

    Benavente, M.; Fresno Baquero, M.R.; Álvarez, S.; Darmanin, N.; Delgado-Bermejo, J.V.
    Resumen de la comunicación presentada al III Congreso Ibérico sobre Recursos Genéticos Animales

  19. Desarrollo testicular en los machos de la Agrupación Caprina Canaria y relación con los cambios estacionales

    Benavente, M.; Fresno Baquero, M.R.; Álvarez, S.; Darmanin, N.; Delgado-Bermejo, J.V.
    Resumen de la comunicación presentada al III Congreso Ibérico sobre Recursos Genéticos Animales

  20. Is the Murciano-Granadina a single goat breed? A molecular genetics approach

    Martínez Martínez, Amparo; Vega-Pla, J.L.; Camacho Vallejo, M.E.; Ribeiro, M.N.; León Jurado, J.M.; Delgado-Bermejo, J.V.
    The population structure of the Murciano-Granadina breed was determined using 25 microsatellites from 266 goats of seven populations. The results of the genetic differentiation analysis showed that it is possible to differentiate the Murciana and Granadina populations even though a low FST value (0.0432) had been obtained. Individuals could be assigned to their populations with a success rate of more than 80%. Bayesian-based clustering analysis of allele frequencies and multivariate analysis revealed that Murciana and Granadina populations were grouped in different clusters since K=3. The results demonstrate that Murciana and Granadina are still two different genetic groups included into Murciano-Granadina...

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