González-García, M.A.; Arenas-Casas, Antonio; Carbonero-Martínez, Alfonso; Borge-Rodríguez, Carmen; García-Bocanegra, Ignacio; Maldonado, J.L.; Gómez-Pacheco, Juan M.; Perea-Remujo, J.A.
An epidemiological and serological survey of bovine herpesvirus 1 (BHV1) infection was conducted in Andalusia from
January to April of 2000. A total of 4,035 blood samples were collected from 164 herds. A questionnaire, which included
variables potentially associated with infection, was filled out for each herd. Serum samples were obtained to identify specific
BHV1 antibodies and were tested using a blocking ELISA test. The observed crude odds ratio (OR) (estimate of the
chance of a particular event occurring in an exposed group in relation to its rate of occurrence in a nonexposed group) for
vaccination is 9.8 (95 % confidence interval: 8.3-11.7). The vaccinated...
Rodríguez-Ortega, Manuel J.; Luque-Moreno, Inmaculada; Tarradas-Iglesias, Carmen; Bárcena, J.A.
Background: Annotation of protein-coding genes is a key step in sequencing projects. Protein
functions are mainly assigned on the basis of the amino acid sequence alone by searching of
homologous proteins. However, fully automated annotation processes often lead to wrong
prediction of protein functions, and therefore time-intensive manual curation is often essential.
Here we describe a fast and reliable way to correct function annotation in sequencing projects,
focusing on surface proteomes. We use a proteomics approach, previously proven to be very
powerful for identifying new vaccine candidates against Gram-positive pathogens. It consists of
shaving the surface of intact cells with two proteases, the specific cleavage-site trypsin and...
Cabezón, Oscar; García-Bocanegra, Ignacio; Molina-López, Rafael; Marco, Ignasi; Blanco, Juan M.; Höfle, Ursula; Margalida, Antoni; Bach-Raich, Esther; Darwich, Laila; Echeverría, Israel; Obón, Elena; Hernández, Mauro; Lavín, Santiago; Dubey, Jitender P.; Almería, Sonia
Toxoplasma gondii is a zoonotic intracellular protozoan parasite of worldwide distribution that infects many species of
warm-blooded animals, including birds. To date, there is scant information about the seropositivity of T. gondii and the risk
factors associated with T. gondii infection in wild bird populations. In the present study, T. gondii infection was evaluated on
sera obtained from 1079 wild birds belonging to 56 species (including Falconiformes (n = 610), Strigiformes (n = 260),
Ciconiiformes (n = 156), Gruiformes (n = 21), and other orders (n = 32), from different areas of Spain. Antibodies to T. gondii
(modified agglutination test, MAT titer $1:25) were found...
Collado-Romero, Melania; Arce Jiménez, Cristina; Ramírez-Boo, M.; Carvajal, Ana; Garrido, Juan J.
Salmonella enterica serovar Typhimurium causes enteric disease and compromises food safety.
In pigs, the molecular response of the intestine to S. typhimurium has been traditionally characterized by
in vitro models that do not reflect the actual immunological competence of the intestinal mucosa. In this
work, we performed an oral S. typhimurium infection study to obtain insight into the in vitro response in
three different sections (jejunum, ileum and colon) of the porcine intestine. For this, samples from onemonth-
old infected piglets were collected during a time course comprising 1, 2, and 6 days post inoculation
to evaluate the intestinal response by quantifying the mRNA expression of...
Alba, Anna; Bicout, Dominique J.; Vidal, Francesc; Curcó, Antoni; Allepuz, Alberto; Napp, Sebastián; García-Bocanegra, Ignacio; Costa, Taiana; Casal, Jordi
Design, sampling and data interpretation constitute an important challenge for wildlife surveillance of avian influenza
viruses (AIV). The aim of this study was to construct a model to improve and enhance identification in both different periods
and locations of avian species likely at high risk of contact with AIV in a specific wetland. This study presents an individualbased
stochastic model for the Ebre Delta as an example of this appliance. Based on the Monte-Carlo method, the model
simulates the dynamics of the spread of AIV among wild birds in a natural park following introduction of an infected bird.
Data on wild bird species population, apparent...
Gómez-Gascón, Lidia; Olaya-Abril, Alfonso; Jiménez-Munguía, I.; Luque-Moreno, Inmaculada; Tarradas-Iglesias, Carmen; Bárcena, J.A.; Rodríguez-Ortega, Manuel J.
Comunicaciones a congresos
LaCourse, E. James; Perally, Samirah; Morphew, Russell M.; Moxon, Joseph V.; Prescott, Mark; Dowling, David J.; O'Neill, Sandra M.; Kipar, Anja; Hetzel, Udo; Hoey, Elizabeth; Brophy, Peter M.; Pérez, José; Zafra Leva, Rafael; Buffoni Perazzo, Leandro
Background: Liver fluke infection of livestock causes economic losses of over US$ 3 billion worldwide per annum. The
disease is increasing in livestock worldwide and is a re-emerging human disease. There are currently no commercial
vaccines, and only one drug with significant efficacy against adult worms and juveniles. A liver fluke vaccine is deemed
essential as short-lived chemotherapy, which is prone to resistance, is an unsustainable option in both developed and
developing countries. Protein superfamilies have provided a number of leading liver fluke vaccine candidates. A new form
of glutathione transferase (GST) family, Sigma class GST, closely related to a leading Schistosome vaccine candidate (Sm28),
Huerta Lorenzo, Belén; Tarradas-Iglesias, Carmen; González, M.; Peralta, B.; Mateu de Antonio, Enrique María; Astorga Márquez, Rafael J.; Carbonero-Martínez, Alfonso; Luque-Moreno, Inmaculada
En este trabajo se ha determinado la seroprevalencia de IgG frente al genotipo 3 del Virus de la Hepatitis E (VHE) en una muestra representativa de perros de una provincia del sur de España, y se han analizado los posibles factores de riesgo asociados a la seropositividad. Un total de 296 muestras de suero fueron analizadas mediante ELISA. Todas las muestras con una densidad óptica positiva, cerca del punto de corte, fueron confirmados por Western Blot. La seroprevalencia global fue del 15,5% (IC del 95%, 11,42-19,66%). El análisis de los diferentes factores relacionados con el animal y con el ambiente...
Gómez, J.C.; Huerta Lorenzo, Belén; Luque-Moreno, Inmaculada; Maldonado García, Alfonso; Astorga Márquez, Rafael J.; Tarradas-Iglesias, Carmen
Biosecurity can be defined as all the applied measurements that take as a target to minimize the sanitary risks in a stock farm, and include
measurements related to the facilities and the management. The efficacy of these measurements must be reflected in a decrease of the microorganism
in different productive phases. A study was carried out to evaluate if the set of applied measurements influences the microbial uterine contamination
after farrowing of healthy sows. Two swine farms were been completed about biosecurity measurements was completed and a microbiological study of
uterine swabs of sows after the farrowing was carried out. A total of 60...
García-Bocanegra, Ignacio; Pérez de Val, Bernat; Arenas-Montes, Antonio José; Paniagua, Jorge; Boadella, Mariana; Gortázar, Christian; Arenas-Casas, Antonio
The control of bovine tuberculosis (bTB) is at a critical point in the last stage of eradication in livestock. Wildlife species
recently have emerged infected with TB in Europe, particularly ungulates in the Iberian Peninsula. Epidemiological
information regarding TB in wild ungulates including affected species, prevalence, associated risk factors and appropriate
diagnostic methods need to be obtained in these countries. A cross-sectional study was carried out on wild artiodactyl
species, including Eurasian wild boar (Sus scrofa) red deer (Cervus elaphus), roe deer (Capraelus capraelus), fallow deer (Dama
dama), Spanish ibex (Capra pyrenaica hispanica) and mouflon (Ovis musimon), in Spain to assess the seroprevalence against
Pérez-Rendón González, J.; Moreno Montañez, T.; Becerra Martell, C.; Martínez Cruz, Setefilla
Se realiza un estudio sobre seroprevalencia de la
toxoplasmosis humana en Córdoba, mediante inmunofluorescencia
indirecta y hemaglutinación indirecta.
La muestra encuestada se compone de 443 sueros, 356
personas supuestamente sanas (estudiantes fundamentalmente)
y 87, consideradas de “alto riesgo” (enfermos
del Hospital “Reina Sofía”).
La positividad obtenida para el total de la
muestra ha sido del 43,79 % con IFI y 53,50 % para
HAI. Con respecto al sexo, la prevalencia es superior
en mujeres con un 54,36 % y 70,47 % con IFI y HAI,
respectivamente, en varones el 38,43 % y 44,90 %
para las mismas pruebas. En relación con la
procedencia de la muestra, 31,lB % con IFI y 43,25
Arenas-Casas, Antonio; Amaro López, Manuel Ángel; Moreno Rojas, Rafael; Sevillano Morales, Jesús; Pérez Rodríguez, Fernando
El riesgo toxicológico por plomo derivado del consumo de carnes de caza es tolerable,
recomendándose potenciar el consumo de este tipo de carnes por la población general.
Hernández García, Manuela; Creus Gibert, Eva; Amaro López, Manuel Ángel; Luque-Moreno, Inmaculada; Astorga Márquez, Rafael J.; Carrasco Otero, Librado
Astorga Márquez, Rafael J.; Carrasco Otero, Librado
García-Bocanegra, Ignacio; Arenas-Montes, Antonio José; Perea-Remujo, J.A.; Arenas-Casas, Antonio; Torralbo, A.; Borge-Rodríguez, Carmen; Carbonero-Martínez, Alfonso; Maldonado García, Alfonso
An observacional cross-sectional study was performed to determine the risk factors associated to the main viral agents of the bovine respiratory disease: bovine herpesvirus type 1 (HVB1), bovine viral diarrhoea virus (VDVB), bovine respiratory syncytial virus (VRSB) and parainfluenza 3 virus (VPI3). Blood samples from dairy calves in the provinces of Cordova and Santa Fe (Argentina) were obtained, and an epidemiological ques-tionnaire was filled. Antibodies against studied viruses were detected by commercial ELISA kits. A total of 852 blood samples from 55 dairy operations were obtained between years 2000 and 2002. Four epidemiologic logistic regression models were performed. We found...
Astorga Márquez, Rafael J.
Astorga Márquez, Rafael J.
León Vizcaíno, L.; Carranza Guzmán, J.; Miranda García, Antonio; Hermoso de Mendoza, M.; Perea-Remujo, J.A.
A serological inquiry on listeriosis of the domestic ruminants of the province of Cordoba (Spain) was made to know the epizootiology of the disease. The agglutination in tube with 2-mercaptoetanol technique was carried out using somatic antigens of the 1/2 and 4 serological types of Listeria monocytogenes. 2.685 samples of apparently healthy adult ruminants from 286 flocks have been investigated. In sheep we found 10 foci of the infection (11.9 per cent of the investigated flocks). In cattle we detected 15 foci (9.56 per cent). In goats we found 5 foci (10 per cent). In relation to the individuals, in...