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Repositorio Institucional de la Universidad de Cordoba :: Spain (51.670 recursos)

El repositorio recoge todo tipo de materiales digitales: artículos de revistas, comunicaciones a congresos, tesis doctorales, documentos de trabajo, materiales docentes y objetos de aprendizaje, así como los productos digitales del patrimonio bibliográfico de la Universidad de Córdoba.

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Mostrando recursos 1 - 20 de 73

  1. Overcoming function annotation errors in the Gram-positive pathogen Streptococcus suis by a proteomics-driven approach

    Rodríguez-Ortega, Manuel J.; Luque-Moreno, Inmaculada; Tarradas-Iglesias, Carmen; Bárcena, J.A.
    Background: Annotation of protein-coding genes is a key step in sequencing projects. Protein functions are mainly assigned on the basis of the amino acid sequence alone by searching of homologous proteins. However, fully automated annotation processes often lead to wrong prediction of protein functions, and therefore time-intensive manual curation is often essential. Here we describe a fast and reliable way to correct function annotation in sequencing projects, focusing on surface proteomes. We use a proteomics approach, previously proven to be very powerful for identifying new vaccine candidates against Gram-positive pathogens. It consists of shaving the surface of intact cells with two proteases, the specific cleavage-site trypsin and...

  2. Gut microbiota: in sickness and in health

    Huertas, M. José; Michán, Carmen

  3. Spectrophotometric Quantitation of Antioxidant Capacity through the Formation of a Phosphomolybdenum Complex: Specific Application to the Determination of Vitamin E1

    Prieto, Pilar; Pineda Priego, Manuel; Aguilar, Miguel
    A spectrophotometric method has been developed for the quantitative determination of antioxidant capacity. The assay is based on the reduction of Mo(VI)to Mo(V) by the sample analyte and the subsequent formation of a green phosphate/Mo(V) complex atacidic pH. The method has been optimized and characterized with respect to linearity interval, repetitivity and reproducibility, and molar absorption coefficients for the quantitation of several antioxidants, including vitamin E. The phosphomolybdenum method, in combination with hexane monophasic extraction, has also been adapted for the specific determination of vitamin E in seeds. The results obtained with the proposed method were validated by comparison with...

  4. RNA-Seq Analysis of Sulfur-Deprived Chlamydomonas Cells Reveals Aspects of Acclimation Critical for Cell Survival

    González-Ballester, David; Casero, David; Cokus, Shawn; Pellegrini, Matteo; Merchant, Sabeeha S.; Grossman, Arthur R.
    The Chlamydomonas reinhardtii transcriptome was characterized from nutrient-replete and sulfur-depleted wild-type and snrk2.1 mutant cells. This mutant is null for the regulatory Ser-Thr kinase SNRK2.1, which is required for acclimation of the alga to sulfur deprivation. The transcriptome analyses used microarray hybridization and RNA-seq technology. Quantitative RT-PCR evaluation of the results obtained by these techniques showed that RNA-seq reports a larger dynamic range of expression levels than do microarray hybridizations. Transcripts responsive to sulfur deprivation included those encoding proteins involved in sulfur acquisition and assimilation, synthesis of sulfur-containing metabolites, Cys degradation, and sulfur recycling. Furthermore, we noted potential modifications of cellular structures during sulfur deprivation, including...

  5. Reverse genetics in Chlamydomonas: a platform for isolating insertional mutants

    González-Ballester, David; Pootakham, Wirulda; Mus, Florence; Yang, Wenqiang; Catalanotti, Claudia; Magneschi, Leonardo; Montaigu, Amaury de; Higuera, Jose J.; Prior, Matthew; Galván Cejudo, Aurora; Fernández Reyes, Emilio; Grossman, Arthur R.
    A method was developed to identify insertional mutants of Chlamydomonas reinhardtii disrupted for selected target genes. The approach relies on the generation of thousands of transformants followed by PCR-based screenings that allow for identification of strains harboring the introduced marker gene within specific genes of interest. Our results highlight the strengths and limitations of two independent screens that differed in the nature of the marker DNA used (PCR-amplified fragment containing the plasmid-free marker versus entire linearized plasmid with the marker) and in the strategies used to maintain and store transformants.

  6. Identification and Validation of Reference Genes for Transcript Normalization in Strawberry (Fragaria x ananassa) Defense Responses

    Amil-Ruiz, Francisco; Garrido-Gala, José; Blanco Portales, Rosario; Folta, Kevin M.; Muñoz-Blanco, Juan; Caballero Repullo, José Luis
    Strawberry (Fragaria spp) is an emerging model for the development of basic genomics and recombinant DNA studies among rosaceous crops. Functional genomic and molecular studies involve relative quantification of gene expression under experimental conditions of interest. Accuracy and reliability are dependent upon the choice of an optimal reference control transcript. There is no information available on validated endogenous reference genes for use in studies testing strawberrypathogen interactions. Thirteen potential pre-selected strawberry reference genes were tested against different tissues, strawberry cultivars, biotic stresses, ripening and senescent conditions, and SA/JA treatments. Evaluation of reference candidate’s suitability was analyzed by five different methodologies, and information was merged to identify...

  7. Descripción del proteoma de bellota de la encina (Quercus ilex) mediante extracción secuencial de proteínas y análisis por LC-MS

    Romero-Rodríguez, M. Cristina; Jorrín-Novo, Jesús V.; Maldonado-Alconada, Ana M.
    Comunicaciones a congresos

  8. Validación de la técnica de análisis masivo ("shotgun") con péptidos no marcados para el estudio del proteoma de Botrytis cinerea

    González-Fernández, Raquel; Aloria, Kerman; Redondo, Inmaculada; Arizmendi, Jesús M.; Jorrín-Novo, Jesús V.
    Comunicaciones a congresos

  9. Adaptation to potassium starvation of wild-type and K+-transport mutant (trk1,2) of Saccharomyces cerevisiae: 2-dimensional gel electrophoresis-based proteomic approach

    Gelis, Samuel; Curto Rubio, Miguel; Valledor, Luis; González, Asier; Ariño, Joaquín; Jorrín-Novo, Jesús V.; Ramos, José
    Saccharomyces cerevisiaewild-type (BY4741) and the correspondingmutant lacking the plasma membrane main potassium uptake systems (trk1,trk2) were used to analyze the consequences of K+ starvation following a proteomic approach. In order to trigger high-affinity mode of potassium transport, cells were transferred to potassium-freemedium.Protein profilewas followed by two-dimensional (2-D) gels in samples taken at 0, 30, 60, 120, 180, and 300 min during starvation.We observed a general decrease of protein content during starvation that was especially drastic in the mutant strain as it was the case of an important number of proteins involved in glycolysis. On the contrary, we identified proteins related to stress response and alternative energetic metabolism that remained...

  10. Improving top-down proteomics: first steps

    Fuentes, C.; González, R.; Gómez, C.; Ogueta-Villarreal, Samuel
    Comunicaciones a congresos

  11. Identification of non-small cell lung cancer biomarkers using mass spectrometry

    Gámez-Pozo, A.; Sánchez-Navarro, I.; Calvo, Enrique; Agulló, T.; Díaz, E.; Camafeita, Emilio; Espinosa, E.; López, L.A.; Nistal, M.; Fresno Vara, J.A.
    Comunicaciones a congresos

  12. Identification of new immunoprotective surface protein vaccine candidates against streptococcus suis infection by proteomics

    Gómez-Gascón, Lidia; Olaya-Abril, Alfonso; Jiménez-Munguía, I.; Luque-Moreno, Inmaculada; Tarradas-Iglesias, Carmen; Bárcena, J.A.; Rodríguez-Ortega, Manuel J.
    Comunicaciones a congresos

  13. Analysis of the holm oak (Quercu Ilex subs ballota) pollen proteome by using NLC-MS/MS

    Valero, J.; Redondo-López, I.; Navarro Cerrillo, Rafael M.; Ariza-Mateos, David; Arroyo, M.; Marchal Gallardo, F.; Mallofret Carrera, E.; Jorrín-Novo, Jesús V.
    Comunicaciones a congresos

  14. Use of metalloproteomics to access environmental stress in the free-living mice mus spretus

    Gómez-Ariza, José Luis; García-Barrera, Tamara; González-Fernández, M.; García-Sevillano, M.A.; Jara-Biedma, R.; Vioque-Fernández, Amalia; López-Barea, Juan; Pueyo, Carmen
    Comunicaciones a congresos

  15. New clues to metabolic regulation through changes in the thiol redox proteome

    Bárcena, J.A.; McDonagh, B.; Requejo, R.; Pedrajas, J.R.; Padilla, C.A.
    Comunicaciones a congresos

  16. Balance de las II Jornadas Bienales de Jóvenes Investigadores en Proteómica, Córdoba 2010

    Echevarría-Zomeño, Sira; González, Raquel; Vázquez, Jesús; Maldonado-Alconada, Ana M.
    Comunicaciones a congresos

  17. Comparative proteomic analysis of Arabidopsis wild-type, mutants, and Fa WRKY1 transgenic plant to characterize the function fo strawberry (Fragaria X ananassa) Fa WRKY1 protein and its Arabidopsis homolog, at WRKY75 two positive regulators of resistance

    Ruiz-Ramos, Alba; Amil-Ruiz, Francisco; Muñoz-Blanco, Juan; Caballero Repullo, José Luis; Maldonado-Alconada, Ana M.
    Comunicaciones a congresos

  18. Estudio de la respuesta al estrés hídrico en dos poblaciones de encina (Quercus ilex subsp.ballota (Desf.) Samp.) mediante una aproximación de proteómica comparativa basada en electroforesis bidimensional

    Valero-Galván, José; Navarro Cerrillo, Rafael M.; Romero-Rodríguez, M. Cristina; Ariza-Mateos, David; Jorrín-Novo, Jesús V.
    Comunicaciones a congresos

  19. Proteome regulation and epigenetic code during Pinus radiata needle maturation

    Valledor, Luis; Cañal, María Jesús; Lenz, Christof; Rodríguez, Roberto; Jorrín-Novo, Jesús V.
    Comuncaciones a congresos

  20. proteome profile analysis of Medicago truncatula leaves in response to Uromyces striatus

    Castillejo, M. Ángeles; Jorrín-Novo, Jesús V.; Rubiales, Diego
    Comunicaciones a congresos

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