Mostrando recursos 1 - 20 de 1.066

  1. Avaliação de corante fluorescente benzazólico para detecção em parasitos diagnosticados por flutuação simples

    Marques, Sandra Marcia Tietz; Barros, Hélio Lopes Barbosa; Glasenapp, Raquel; Stefani, Valter
    Este estudo mostra o corante fluorescente 4-hidroxi-3- (6- metilbenzo[d]oxazol-2-il) benzeno sulfônico (HB-9) marcando, por microscopia de fluorescência, ovos de helmintos e oocistos de protozoários diagnosticados em animais domésticos. Foram selecionados ovos de Acuaria spiralis, Strongyloides papillosus, Capillaria spp., Bertiella spp., Strongyloidea e Eimeria spp., identificados previamente pelo método de flutuação. As amostras foram observadas em microscópio BEL Photonics INV-100 FL Plus com sistema de Epi-Fluorescência. Ovos de A. spiralis, Capillaria spp., Bertiella spp. e Eimeria spp. apresentaram fluorescência. O corante mostrou ser capaz de marcar as estruturas parasitárias de modo seletivo, facilitando a visualização do material de interesse.

  2. Avaliação e comparação entre métodos de mensuração de pressão arterial sistólica em gatos hígidos conscientes

    Anjos, Tathiana Mourão dos; Veado, Júlio César Cambraia; Araújo, Roberto Baracat de; Leme, Fabiola de Oliveira Paes; Diniz, Soraia de Araujo; Castro, Maria Cristina Nobre e; Costa, Fernanda Vieira Amorim da; Tavares , C. A. P.; David, L. M.; Quites, K. X.; Maia, M. Q.
    Após validação de dois diferentes métodos de mensuração de pressão arterial sistólica (PAS) não invasivos ou indiretos (oscilométrico e Doppler vascular) com o invasivo ou direto (cateterização arterial  padrão ouro) em 12 gatos hígidos anestesiados de mesma faixa etária, os métodos não invasivos foram avaliados e comparados em 24 gatos hígidos e conscientes, divididos em quatro grupos de idade. Em cada animal, procedeu-se à mensuração da pressão pelos métodos Doppler e oscilométrico. O método oscilométrico foi o primeiro a ser realizado, e, logo em seguida, o método Doppler. Os valores considerados foram obtidos das médias de sete medidas consecutivas...

  3. Dispneia expiratória restritiva em um gato com fibrose pulmonar idiopática : relato de caso

    Pereira, Camila de Oliveira; Costa, Fernanda Vieira Amorim da; Gomes, Danilo Carloto; Kasper, Priscila Natasha; Vieira, Aline Bomfim; Driemeier, David
    As doenças pulmonares intersticiais constituem um grupo de doenças difusas do parênquima pulmonar, no qual a fibrose pulmonar intersticial está incluída. Histologicamente, esta se caracteriza por hiperplasia de pneumócitos tipo II, hiperplasia ou hipertrofia de músculo liso e fibrose. Embora a patogenia da fibrose pulmonar intersticial não esteja bem elucidada, devido às semelhanças microscópicas encontradas nos pneumócitos tipo II em felinos e na forma familiar da doença em humanos, acredita-se que haja caráter genético para o seu desenvolvimento. Os sinais clínicos frequentemente relatados incluem desconforto respiratório, cianose, letargia e perda de peso. Devido ao caráter progressivo e à ausência de...

  4. First case of degenerative mucinotic mural folliculitis in Brazil

    Sousa Filho, Reginaldo Pereira de; Rolim, Veronica Machado; Sampaio, Keytyanne de Oliveira; Driemeier, David; Cunha, Marina Gabriela Monteiro Carvalho Mori da; Costa, Fernanda Vieira Amorim da
    The purpose of this report is to describe the first case of degenerative mucinotic mural folliculitis (DMMF) in Brazil. This dermathopathy has been described as a rare cause of alopecia and hyperkeratosis in cats. Severe forms have been linked to infection with Feline Immunodeficiency Virus (FIV). A 10-year-old neutered male Siamese cat was referred with a 40 days history of progressive hair loss. The cat had initial alopecia on the head, subsequently spreading to the neck, torso and limbs. The skin biopsy revealed degenerative mucinotic mural folliculitis. Distinct seropositive reaction against FIV. antigens was demonstrated by enzyme-linked immunosorbent assay and...

  5. Capilariose em savacu (Nycticorax nycticorax)

    Marques, Sandra Marcia Tietz; Silva, Bruna Zafalon da; Boll, Anelise Schuch; Santos, Eduardo Almeida Ruivo dos; Alievi, Marcelo Meller
    Nycticorax nycticorax é uma ave da família Ardeidae. É cosmopolita e com ampla distribuição mundial e as infecções parasitárias estão entre os problemas sanitários mais comuns que as afetam. Este relato objetiva descrever o tratamento parasitário em um savacu com capilariose, em internação no Núcleo de Conservação e Reabilitação de Animais Silvestres -Preservas/ UFRGS, Brasil. Dados sobre tratamento são escassos nesta espécie. O animal era de vida livre, proveniente do Campus Universitário da UFRGS e foi trazido ao hospital por populares. Capillaria spp. foi diagnosticada por dois métodos coprológicos. A ave foi medicada com levamisole (20 mg·kg-1, subcutânea, dose única)....

  6. Anti-inflammatory activity and chemical analysis of extracts from Trifolium riograndense

    Pedrazza, Graziele Pereira Ramos; Morais, Cláudia Borges de; Dettenborn, Greice Raquel; Ceolato, Paula Casagrande; Apel, Miriam Anders; Schapoval, Elfrides Eva Scherman; Dall'Agnol, Miguel; Zuanazzi, Jose Angelo Silveira
    Aiming to investigate new therapeutic agents with fewer side effects, the number of studies about naturalproducts has increased. Phenolic compounds comprise a well-studied class of abundant plant-derivedcompounds, whose anti-inflammatory activity has been described. Isoflavones are phenolic compoundsthat occur mainly in the Leguminosae family, and can be found in many species, such as Trifoliumriograndense Burkart, Leguminosae (clover). In this study an HPLC method was used to determine andquantify four isoflavones (genistein, daidzein, formononetin, and biochanin A) in hydrolyzed leaf, flower,stolon, and root extracts of T. riograndense. In vivo anti-inflammatory activity was investigated using therat paw edema method and in vitro chemotaxis...

  7. Morphology of endothelial cells from different regions of the equine cornea

    Faganello, Cláudia Skilhan; Silva, Vanessa Ruiz Moura da; Andrade, Maria Cristina Caldart de; Carissimi, Andre Silva; Pigatto, João Antonio Tadeu
    The objective of this study was to evaluate the morphology of different regions of the equine cornea using optical microscopy. Both healthy eyes of eight horses, male or female, of different ages were evaluated. Corneas were stained with alizarin red vital dye and subsequently examined and photographed using optical microscopy. Corneal endothelial morphology of central, superior, inferior, temporal and nasal areas was assessed. One hundred endothelial cells from each corneal area were analyzed. The shape of the corneal endothelial cells of each corneal region was analyzed. Statistical data analysis was conducted using the Student’s t test. Values of P<0.01 were...

  8. Effects of intracameral brilliant blue on the corneal endothelium of swine : in vitro study

    Barbosa, Mariana Terzariol; Hünning, Paula Stieven; Brambatti, Gustavo; Albuquerque, Luciane; Neumann, Carolina Fonseca; Pigatto, João Antonio Tadeu
    The aim was to investigate the ultrastructural changes in the corneal endothelium of pigs induced by intracameral 0.05% brilliant blue. Twenty swine corneas were separated into two groups, the right eye bulbs (control group) and the left eye bulbs (experimental group) of the same animal. All the eye bulbs were evaluated with specular microscopy. The cornea of the right eye bulbs was excised and in the left eye bulbs 0.2ml of 0.05% brilliant blue vital dye (OPTH-blue®) was injected into the anterior chamber, where it remained for one minute. Then the anterior chamber was cleaned with a balanced salt solution...

  9. Conjunctival melanoma in a horse treated by tumor resection and cryotherapy

    Pigatto, João Antonio Tadeu; Vargas, Eduarda Valim Borges de; Torikachvili, Marcela; Albuquerque, Luciane; Andrade, Maria Cristina Caldart de; Santos, Elisandro Oliveira dos; Driemeier, David
    Background: Ocular melanoma is very rare compared to cutaneous melanoma in horses. Definitive diagnosis is made through histopathological examination and treatment options include surgical excision associated with cryotherapy, radiation therapy, and chemotherapy. In this report, we describe a case of conjunctival melanoma in a horse that has been treated successfully with surgical excision associated with cryotherapy. Case: A 15-year-old male Percheron male was referred to the Ophthalmology Veterinary Section of the Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Brazil, with a history of a pigmented mass located on the lower eyelid of the left eye. Ophthalmologic examination...

  10. Collection season and auxin treatment in the propagation by cuttings of mandarin hybrids

    Sarmiento, Andres Ivan Pinto; Souza, Paulo Vitor Dutra de; Schwarz, Sergio Francisco
    Propagation by cuttings is an alternative for obtaining citrus rootstocks. The exogenous application of indolebutyric acid (IBA), cutting collection season and genotypes used may affect the rooting and vegetative growth of citrus rootstocks cuttings. Two experiments were conducted to study the influence of these factors. In the first one, semi-hardwood cuttings from the ‘Sunki’ mandarin hybrids H49 and H77 were collected in the fall and late spring of 2013 and treated with IBA (0 mg L-1, 1,500 mg L-1 and 3,000 mg L-1). For each collection season, a 2 x 3 (two genotypes x three IBA concentrations) factorial scheme was...

  11. Cell therapy in the treatment of bipolar mania in an animal model : a proof of concept study

    Ascoli, Bruna Maria; Colombo, Rafael; Géa, Luíza Paul; Terraciano, Paula Barros; Pizzato, Sabrina Beal; Oliveira, Fernanda Santos de; Cirne Lima, Elizabeth Obino; Kapczinski, Flávio Pereira; Rosa, Adriane Ribeiro
    Introduction: The rationale of mesenchymal stem cells (MSCs) as a novel therapeutic approach in certain neurodegenerative diseases is based on their ability to promote neurogenesis. Hippocampal atrophy has been related to bipolar disorder (BD) in preclinical, imaging and postmortem studies. Therefore, the development of new strategies to stimulate the neurogenesis process in BD is crucial. Objectives: To investigate the behavioral and neurochemical changes induced by transplantation of MSCs in a model of manialike behavior induced by lisdexamfetamine dimesylate (LDX). Methods: Wistar rats (n=65) received one oral daily dose of LDX (10 mg/kg) or saline for 14 days. On the 8th...

  12. Genetic and cytological diversity in cherry tree accessions ( Eugenia involucrata DC) in Rio Grande do Sul

    Guerra, Divanilde; Souza, Paulo Vitor Dutra de; Schwarz, Sergio Francisco; Schifino-Wittmann, Maria Teresa; Werlang, Claudio André; Veit, Pedro Augusto
    This study aimed to evaluate the genetic and cytological diversity and stability of 35 cherry tree accessions collected in Rio Grande do Sul. We used 15 RAPD (Random Amplified Polymorphic DNA) molecular markers and performed cytological analysis and number count of anthers. Analyses of genetic diversity allowed the separation of accessions into four groups, resulting in an average of 8.93 bands per primer amplified, 7.89 polymorphic bands, 88.08% of polymorphism and 86% of genetic similarity. Cytological analyses of gametic cells allowed for the characterization of accessions as diploids with n=11. In these, the average of meiotic cells considered normal was...

  13. Root distribution of peach rootstocks affected by soil compaction and acidity

    Petry, Henrique Belmonte; Mazurana, Michael; Marodin, Gilmar Arduino Bettio; Levien, Renato; Anghinoni, Ibanor; Gianello, Clesio; Schwarz, Sergio Francisco
    Root growth can be limited by physical and chemical conditions of the soil. Compacted and acidic soils, where there is an occurrence of exchangeable Al, constitute barriers to use of the soil by plant roots. The hypothesis of this study was that physical and chemical properties of the inter-row soil of a peach orchard influence the root distribution of different rootstocks. The aim of this study was to describe and register the soil physical and chemical properties and root distribution in the soil profile of the inter-row of seven years old mature peach (Prunus persica) orchard. Samples of soil (classified...

  14. 3,5,6-trichloro-2-pyridinyloxyacetic acid as effective thinning agent for fruit of 'Montenegrina' mandarin

    Gonzatto, Mateus Pereira; Boettcher, Gerson Nestor; Schneider, Leonardo André; Lopes, Angelo Azeredo; Silveira Júnior, Jairo Camargo; Petry, Henrique Belmonte; Oliveira, Roberto Pedroso de; Schwarz, Sergio Francisco
    The ‘Montenegrina’ mandarin (Citrus deliciosa Tenore) is widely cultivated in southern Brazil. This cultivar has a great tendency for alternate bearing, requiring thining of the fruit. However, the chemical thinners studied until now in this cultivar have not been successful. The objective of this research was to evaluate the effect of 3,5,6-trichloro-2-pyridinyloxyacetic acid (3,5,6-TPA) on the production and fruit quality of the ‘Montenegrina’ mandarin, in comparison with hand thinning and the options of chemical thinning previously studied. Hand thinning and no thinning were used as controls. Ethephon was applied in three doses: 200mg L-1, 300mg L-1 or 200mg L-1 +...

  15. Biofilm formation capacity of Salmonella serotypes at different temperature conditions

    Borges, Karen Apellanis; Furian, Thales Quedi; Souza, Sara Neves de; Menezes, Rafaela; Tondo, Eduardo Cesar; Salle, Carlos Tadeu Pippi; Moraes, Hamilton Luiz de Souza; Nascimento, Vladimir Pinheiro do
    Salmonella spp. são um dos mais importantes agentes causadores de doenças transmitidas por alimentos em vários países, inclusive no Brasil.Produtos avícolas e ovos são os principais alimentos envolvidos na transmissão dos sorovares de Salmonella que são responsáveis por surtos de salmonelose em humanos. Salmonella possui a capacidade de formar biofilmes em diversas superfícies. O processo de formação de biofilme depende da interação entre as células bacterianas, a superfície de adesão e as condições do ambiente onde a bactéria se encontra. Estas estruturas favorecem a sobrevivência bacteriana em ambientes hostis, como em matadouros-frigoríficos e em indústrias processadoras de alimentos. Biofilmes são...

  16. A proteomic insight into vitellogenesis during tick ovary maturation

    Xavier, Marina Amaral; Tirloni, Lucas; Pinto, Antonio Frederico Michel; Diedrich, Jolene K.; Yates III, John R.; Mulenga, Albert; Logullo, Carlos; Vaz Junior, Itabajara da Silva; Seixas, Adriana; Termignoni, Carlos
    Ticks are arthropod ectoparasites of importance for public and veterinary health. The understanding of tick oogenesis and embryogenesis could contribute to the development of novel control methods. However, to date, studies on the temporal dynamics of proteins during ovary development were not reported. In the present study we followed protein profile during ovary maturation. Proteomic analysis of ovary extracts was performed by liquid chromatography-tandem mass spectrometry (LC-MS/ MS) using shotgun strategy, in addition to dimethyl labelling-based protein quantification. A total of 3,756 proteins were identified, which were functionally annotated into 30 categories. Circa 80% of the annotated proteins belong to...

  17. Condiciones de cultivo de la planta matriz y uso del ácido indolbutírico en la propagación del mandarino 'Sunki' por estaquilla

    Sarmiento, Andres Ivan Prato; Schwarz, Sergio Francisco; Souza, Paulo Vitor Dutra de
    Los patrones se obtienen tradicionalmente en la citricultura a partir de semillas, seleccionándose las plantas de origen apomíctica. Sin embargo, hay riesgo de no realizarse la selección correcta, debido a la baja apomixis y la alta heterocigocidad en algunas especies o cultivares, como el mandarino ‘Sunki’. La obtención de los mismos por estaquillas solucionaría el problema. El objetivo de este trabajo fue evaluar el potencial de enraizamiento de estaquillas del mandarino ‘Sunki’ (Citrus sunki Hort. ex Tan.), colectadas de matrices cultivadas en el campo o en ambiente protegido, utilizándose diferentes concentraciones del ácido indolbutírico (AIB). Las estaquillas se colectaron en...

  18. Lip twitch restraint on rebound tonometry in horses

    Andrade, Maria Cristina Caldart de; Hünning, Paula Stieven; Pereira, Fabiane Cristina; Dutra, Kadigia Pitol; Pigatto, João Antonio Tadeu
    The purpose of this study was to verify the effect of the upper lip twitch restraint on intraocular pressure (IOP) of healthy horses. In this study, forty five Criollo horses, aged between two to 20 years (male or female) were evaluated with rebound tonometer, with and without upper lip twitch restraint. A previous ophthalmic examination was performed with Schirmer tear test, fluorescein test, slit lamp biomicroscopy and direct ophthalmoscopy in all horses. Only healthy animals with no ocular findings were used. The order of the IOP measurements (with or without twitch) and order of the eye (right or left) were...

  19. Corneal endothelial cell density and morphology in rabbits’ eyes using contact specular microscopy

    Brambatti, Gustavo; Albuquerque, Luciane; Vargas, Eduarda Valim Borges de; Neumann, Carolina Fonseca; Pigatto, João Antonio Tadeu
    The goal of this study was to describe the normal values of corneal endothelial cell density and hexagonality in healthy rabbits’ eyes using contact specular microscopy. Both eyes of 18 rabbits, males and females, of different ages were evaluated. The eyes were divided into three groups of 12 each according to the age range of the animals: G1 (6 months old), G2 (12 months old), and G3 (48 months old). Parameters studied included mean endothelial cell density and hexagonality. For Group I, the mean cell density was 2307 cells per mm2. For GII, the mean cell density was 1895 cells...

  20. Assessment of intraocular pressure in chinchillas of different age groups using rebound tonometry

    Chacaltana, Flor Diana; Pigatto, João Antonio Tadeu; Denardin, Ione T.
    The aim of this research was to measure the intraocular pressure (IOP) of normal chinchilla eyes using the rebound tonometer. A further aim was to assess whether there were differences in the values of intraocular pressure in relation to animals age, gender and time of day. Thirty-six chinchillas were divided into three groups of 12 chinchillas each, by age: Group I (2-6-month-old), Group II (20 and 34 months) and Group III (37 and 135 months). Ophthalmic examination was performed previously by Schirmer tear test, slit lamp biomicroscopy, indirect ophthalmoscopy and fluorescein test in all chinchillas. Three measurements of intraocular pressure...

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