Mostrando recursos 1 - 20 de 129

  1. Factors influencing transfection efficiency of pIDUA/nanoemulsion complexes in a mucopolysaccharidosis type I murine model

    Fraga, Michelle; Carvalho, Talita Giacomet de; Bidone, Juliana; Schuh, Roselena Silvestri; Matte, Ursula da Silveira; Teixeira, Helder Ferreira
    Mucopolysaccharidosis type I (MPS I) is an autosomal disease caused by alpha-l-iduronidase (IDUA) deficiency. This study used IDUA knockout mice as a model to evaluate whether parameters such as dose of plasmid and time of treatment could influence the transfection efficiency of complexes formed with PEGylated cationic nanoemulsions and plasmid (pIDUA), which contains the gene that encodes for IDUA. Formulations were composed of medium chain triglycerides, 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine, 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-(amino[polyethylene glycol]-2000), 1,2-dioleoyl-sn-glycero-3-trimethylammonium propane (DOTAP), glycerol, and water and were prepared by the adsorption or encapsulation of preformed pIDUA–DOTAP complexes by high-pressure homogenization. A progressive increase in IDUA expression was observed with an...

  2. Brazilian neurosciences research areas : a bibliometric analysis from 2006 to 2013

    Hoppen, Natascha Helena Franz; Souza, Claudia Daniele de; Filippo, Daniela de; Vanz, Samile Andrea de Souza; Sanz Casado, Elias
    Bibliometrics is used to examine the different branches of Brazilian Neurosciences research, the annual advancement of specialization, and the relationship between scientific categories and author productivity through 9655 articles published on Web of Science from 2006 to 2013. Seventy-one areas are identified that form part of the Transdisciplinarity of Brazilian Neurosciences scientific output as well as the division between clinical research and basic and experimental research. The three most important areas identified were Neurosciences (higher frequency and co-occurrence, especially with “experimental” subjects), Psychiatry (present in the first three years of the survey) and Clinical Neurology (associated with other medical subjects,...

  3. BRCA1 and BRCA2 mutational profile and prevalence in hereditary breast and ovarian cancer (HBOC) probands from Southern Brazil : are international testing criteria appropriate for this specific population?

    Temes, Bárbara Alemar Beserra; Silva, Cleandra Gregório; Herzog, Josef; Bittar, Camila Matzenbacher; Netto, Cristina Brinckmann Oliveira; Artigalas, Osvaldo Alfonso Pinto; Schwartz, Ida Vanessa Doederlein; Coffa, Jordy; Camey, Suzi Alves; Weitzel, Jeffrey; Prolla, Patrícia Ashton
    Background Germline pathogenic variants in BRCA1 and BRCA2 (BRCA) are the main cause of Hereditary Breast and Ovarian Cancer syndrome (HBOC). Methods In this study we evaluated the mutational profile and prevalence of BRCA pathogenic/likely pathogenic variants among probands fulfilling the NCCN HBOC testing criteria. We characterized the clinical profile of these individuals and explored the performance of international testing criteria. Results A pathogenic/likely pathogenic variant was detected in 19.1% of 418 probands, including seven novel frameshift variants. Variants of uncertain significance were found in 5.7% of individuals. We evaluated 50 testing criteria and mutation probability algorithms. There was a...

  4. Morfodinâmica praial : uma breve revisão

    Calliari, Lauro Julio; Muehe, Dieter Carl Ernst Heino; Hoefel, Fernanda Gemael; Toldo Junior, Elirio Ernestino
    Desde o início dos anos 30 perfis de praia foram classificados em um dos dois tipos: perfis de tempestade ou perfis de ondulação associados, respectivamente, com ondas de alta e baixa esbeltez. Mudanças no perfil praial envolvem muitas variáveis, tornando muito difícil a formulação de modelos de prediçâo de comportamento praial. Somente a partir da década de 70, as características dos sedimentos c os processos de transformação de ondas operantes na plataforma interna e antepraia, acoplados com a morfologia tridimensional e a hidrodinâmica da praia, começaram a ser estudados de maneira integrada. Tais estudos levaram a um melhor entendimento do...

  5. Morfodinâmica praial : uma breve revisão

    Calliari, Lauro Julio; Muehe, Dieter Carl Ernst Heino; Hoefel, Fernanda Gemael; Toldo Junior, Elirio Ernestino
    Desde o início dos anos 30 perfis de praia foram classificados em um dos dois tipos: perfis de tempestade ou perfis de ondulação associados, respectivamente, com ondas de alta e baixa esbeltez. Mudanças no perfil praial envolvem muitas variáveis, tornando muito difícil a formulação de modelos de prediçâo de comportamento praial. Somente a partir da década de 70, as características dos sedimentos c os processos de transformação de ondas operantes na plataforma interna e antepraia, acoplados com a morfologia tridimensional e a hidrodinâmica da praia, começaram a ser estudados de maneira integrada. Tais estudos levaram a um melhor entendimento do...

  6. Checklist das Spermatophyta do Estado de São Paulo, Brasil

    Wanderley, Maria das Graças Lapa; Shepherd, George John; Martins, Suzana Ehlin; Estrada, Tiago Egger Moellwald Duque; Romanini, Rebeca Politano; Koch, Ingrid; Pirani, Jose Rubens; Melhem, Therezinha Sant´Anna; Harley, Ana Maria Giulietti; Kinoshita, Luiza Sumiko; Magenta, Mara Angelina Galvão; Longhi-Wagner, Hilda Maria; Barros, Fabio de; Lohmann, Lúcia Garcez; Amaral, Maria do Carmo Estanislau do; Cordeiro, Inês; Aragaki, Sonia; Bianchini, Rosangela Simão; Esteves, Gerleni Lopes
    The Phanerogamic Flora of São Paulo State project has been dedicated to create an inventory of the flora for 20 years. More than 200 collaborators are involved, mostly from the State of São Paulo, with the contributions of researchers from other states and from abroad. Since 2001, seven books with monographs of 151 families were published, dealing with 3,237 species within 722 genera. This project was the starting-point of the gathering of information about the diversity of spermatophytes of the State of São Paulo. The current checklist, at this point, presents an updated and virtually complete list of species, all...

  7. Checklist das Spermatophyta do Estado de São Paulo, Brasil

    Wanderley, Maria das Graças Lapa; Shepherd, George John; Martins, Suzana Ehlin; Estrada, Tiago Egger Moellwald Duque; Romanini, Rebeca Politano; Koch, Ingrid; Pirani, Jose Rubens; Melhem, Therezinha Sant´Anna; Harley, Ana Maria Giulietti; Kinoshita, Luiza Sumiko; Magenta, Mara Angelina Galvão; Longhi-Wagner, Hilda Maria; Barros, Fabio de; Lohmann, Lúcia Garcez; Amaral, Maria do Carmo Estanislau do; Cordeiro, Inês; Aragaki, Sonia; Bianchini, Rosangela Simão; Esteves, Gerleni Lopes
    The Phanerogamic Flora of São Paulo State project has been dedicated to create an inventory of the flora for 20 years. More than 200 collaborators are involved, mostly from the State of São Paulo, with the contributions of researchers from other states and from abroad. Since 2001, seven books with monographs of 151 families were published, dealing with 3,237 species within 722 genera. This project was the starting-point of the gathering of information about the diversity of spermatophytes of the State of São Paulo. The current checklist, at this point, presents an updated and virtually complete list of species, all...

  8. Contribuições ao conhecimento dos gêneros Elanela e Nocheta (Heteroptera, Pentatomini)

    Grazia, Jocelia
    The male of Elanela hevera Rolston et alii, 1980 and the female of Nocheta adda Rolston et alii, 1980 are described for the first time. A complete study of external genitalia for both sexes of E. hevera and N. adda is also presented.

  9. Contribuições ao conhecimento dos gêneros Elanela e Nocheta (Heteroptera, Pentatomini)

    Grazia, Jocelia
    The male of Elanela hevera Rolston et alii, 1980 and the female of Nocheta adda Rolston et alii, 1980 are described for the first time. A complete study of external genitalia for both sexes of E. hevera and N. adda is also presented.

  10. Citogenética de espécies arbóreas da subfamília Caesalpinioideae - Leguminosae do sul do Brasil

    Biondo, Elaine; Miotto, Silvia Teresinha Sfoggia; Schifino-Wittmann, Maria Teresa
    Determinação do número de cromossomos e análise do comportamento meiótico são excelentes contribuições aos estudos de relações taxonômicas e padrões evolutivos dentro de grupos de espécies vegetais. Espécies de leguminosas arbóreas da subfamília Caesalpinioideae, ocorrentes na Região Sul do Brasil, têm sido pouco analisadas do ponto de vista citotaxonômico. Este trabalho teve por objetivos determinar o número de cromossomos e analisar o comportamento meiótico de onze espécies arbóreas dessa subfamília. O número de cromossomos na maioria das espécies analisadas foi 2n = 28 cromossomos (x = 14). Foi encontrado 2n = 24 (x = 12) em Senna multijuga (L.C. Rich.)...

  11. Citogenética de espécies arbóreas da subfamília Caesalpinioideae - Leguminosae do sul do Brasil

    Biondo, Elaine; Miotto, Silvia Teresinha Sfoggia; Schifino-Wittmann, Maria Teresa
    Determinação do número de cromossomos e análise do comportamento meiótico são excelentes contribuições aos estudos de relações taxonômicas e padrões evolutivos dentro de grupos de espécies vegetais. Espécies de leguminosas arbóreas da subfamília Caesalpinioideae, ocorrentes na Região Sul do Brasil, têm sido pouco analisadas do ponto de vista citotaxonômico. Este trabalho teve por objetivos determinar o número de cromossomos e analisar o comportamento meiótico de onze espécies arbóreas dessa subfamília. O número de cromossomos na maioria das espécies analisadas foi 2n = 28 cromossomos (x = 14). Foi encontrado 2n = 24 (x = 12) em Senna multijuga (L.C. Rich.)...

  12. Environmental factors related to the production of a complex set of spicules in a tropical freshwater sponge

    Matteuzzo, Marcela Camargo; Ribeiro, Cecilia Volkmer; Vajarão, Angelica Fortes Drummond Chicarino; Vajarão, Cesar Augusto Chicarino; Alexandre, Anne; Guadagnin, Demétrio Luís; Almeida, Ariana Cristina Santos
    Adverse natural conditions will, generally, induce gemmulation in freshwater sponges. Because of this environmental dependence, gemmoscleres are given exceptional value in taxonomic, ecological and paleoenvironmental studies. Other spicules categories such as microscleres and beta megascleres have received little attention with regard to their occurrence and function during the sponge biological cycle. Metania spinata, a South American species common to bog waters in the Cerrado biome, produces alpha and beta megascleres, microscleres and gemmoscleres. To detect the environmental factors triggering the production of all these kinds of spicules, the species annual seasonal cycle was studied. Artificial substrates were devised, supplied with...

  13. Diversity and evolution of plant diacylglycerol acyltransferase (DGATs) unveiled by phylogenetic, gene structure and expression analyses

    Zolet, Andreia Carina Turchetto; Christoff, Ana Paula; Kulcheski, Franceli Rodrigues; Morais, Guilherme Loss de; Margis, Rogerio; Margis-Pinheiro, Márcia
    Since the first diacylglycerol acyltransferase (DGAT) gene was characterized in plants, a number of studies have focused on understanding the role of DGAT activity in plant triacylglycerol (TAG) biosynthesis. DGAT enzyme is essential in controlling TAGs synthesis and is encoded by different genes.DGAT1 andDGAT2 are the two major types of DGATs and have been well characterized in many plants. On the other hand, the DGAT3 and WS/DGAT have received less attention. In this study, we present the first general view of the presence of putative DGAT3 and WS/DGAT in several plant species and report on the diversity and evolution of...

  14. Bloom of Noctiluca scintillans (Macartney) Kofoid & Swezy (Dinophyceae) in southern Brazil

    Cardoso, Luciana de Souza
    Resumo não disponível

  15. Dynamic and resuspension by waves and sedimentation pattern definition in low energy environments Guaíba Lakes (Brazil).

    Nicolodi, João Luiz; Toldo Junior, Elirio Ernestino; Farina, Leandro
    Little research has been undertaken into sediment dynamics in lakes, and most of it only analyses particular aspects such as the texture of the sediments. In this study, the characteristics of the wave field in Guaíba Lake are investigated. The parameters significant wave height (Hs), period (T) and direction of wave propagation are examined together with their relation to the resuspension of sediments at the bottom. For this purpose, the mathematical model SWAN (Simulating Waves Nearshore) has been validated and employed. The results pointed out that the highest waves modeled reached 0.55 m at a few points in the lake,...

  16. Contribution of WUSCHEL : related homeobox (WOX) genes to identify the phylogenetic relationships among Petunia species

    Segatto, Ana Lúcia Anversa; Thompson, Claudia Elizabeth; Freitas, Loreta Brandao de
    Developmental genes are believed to contribute to major changes during plant evolution, from infrageneric to higher levels. Due to their putative high sequence conservation, developmental genes are rarely used as molecular markers, and few studies including these sequences at low taxonomic levels exist. WUSCHEL-related homeobox genes (WOX) are transcription factors exclusively present in plants and are involved in developmental processes. In this study, we characterized the infrageneric genetic variation of Petunia WOX genes. We obtained phylogenetic relationships consistent with other phylogenies based on nuclear markers, but with higher statistical support, resolution in terminals, and compatibility with flower morphological changes.

  17. Teratogens : a public health issue - a brazilian overview

    Nascimento, Thiago Mazzu; Melo, Débora Gusmão; Morbioli, Giorgio Gianini; Carrilho, Emanuel; Vianna, Fernanda Sales Luiz; Silva, André Anjos da; Faccini, Lavinia Schuler
    Congenital anomalies are already the second cause of infant mortality in Brazil, as in many other middle-income countries in Latin America. Birth defects are a result of both genetic and environmental factors, but a multifactorial etiology has been more frequently observed. Here, we address the environmental causes of birth defects – or teratogens – as a public health issue and present their mechanisms of action, categories and their respective maternal- fetal deleterious effects. We also present a survey from 2008 to 2013 of Brazilian cases involving congenital anomalies (annual average of 20,205), fetal deaths (annual average of 1,530), infant hospitalizations...

  18. Teratogens : a public health issue - a brazilian overview

    Nascimento, Thiago Mazzu; Melo, Débora Gusmão; Morbioli, Giorgio Gianini; Carrilho, Emanuel; Vianna, Fernanda Sales Luiz; Silva, André Anjos da; Faccini, Lavinia Schuler
    Congenital anomalies are already the second cause of infant mortality in Brazil, as in many other middle-income countries in Latin America. Birth defects are a result of both genetic and environmental factors, but a multifactorial etiology has been more frequently observed. Here, we address the environmental causes of birth defects – or teratogens – as a public health issue and present their mechanisms of action, categories and their respective maternal- fetal deleterious effects. We also present a survey from 2008 to 2013 of Brazilian cases involving congenital anomalies (annual average of 20,205), fetal deaths (annual average of 1,530), infant hospitalizations...

  19. New species of Scleromystax Günther, 1864 (Siluriformes:Callichthyidae) : extending the meridional distribution of genera endemic to the Atlantic Forest

    Britto, Marcelo Ribeiro de; Fukakusa, Clayton Kunio; Malabarba, Luiz Roberto
    The genus Scleromystax includes species occurring in several coastal river basins from southern Bahia to southern Santa Catarina States. Examination of Scleromystax specimens sampled from the laguna dos Patos drainage revealed a new taxon, further extending the distribution of the genus to the southern border of the Atlantic forest. The new species differs from its congeners by the second infraorbital ventrally expanded. It is also distinct, except from S. macropterus and S. salmacis, by roughly oblique, elongate, dark brown blotches along body; large specimens displaying cranial fontanel completely occluded, leaving just a shallow fossae; and sexually dimorphic features inconspicuous, i.e....

  20. New species of Scleromystax Günther, 1864 (Siluriformes:Callichthyidae) : extending the meridional distribution of genera endemic to the Atlantic Forest

    Britto, Marcelo Ribeiro de; Fukakusa, Clayton Kunio; Malabarba, Luiz Roberto
    The genus Scleromystax includes species occurring in several coastal river basins from southern Bahia to southern Santa Catarina States. Examination of Scleromystax specimens sampled from the laguna dos Patos drainage revealed a new taxon, further extending the distribution of the genus to the southern border of the Atlantic forest. The new species differs from its congeners by the second infraorbital ventrally expanded. It is also distinct, except from S. macropterus and S. salmacis, by roughly oblique, elongate, dark brown blotches along body; large specimens displaying cranial fontanel completely occluded, leaving just a shallow fossae; and sexually dimorphic features inconspicuous, i.e....

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