Mostrando recursos 1 - 20 de 34

  1. Poor glycaemic control in Brazilian patients with type 2 diabetes attending the public healthcare system : a cross-sectional study

    Viana, Luciana Verçoza; Leitão, Cristiane Bauermann; Kramer, Caroline Kaercher; Zucatti, Alessandra Teixeira Netto; Jezini, Deborah Laredo; Felicio, João Soares; Mendes, Ana Beatriz Valverde; Chacra, Antonio Roberto; Azevedo, Mirela Jobim de; Gross, Jorge Luiz
    Objectives: To describe the clinical profile of Brazilian patients with type 2 diabetes attending the public healthcare system and identify factors associated with poor glycaemic control. Design: Cross-sectional study. Setting: 14 centres in five regions of Brazil, including primary care units and outpatient clinics of University Hospitals. Participants: Patients with type 2 diabetes attending outpatient clinics of public healthcare system. Main outcome measured: Glycated haemoglobin (HbA1c), centrally measured by high-performance liquid chromatography (National Glycohemoglobin Standardization Program certified). Results: A total of 5750 patients aged 61±10 years, with 11±8 years of diabetes duration (66% women, 56% nonwhite, body mass index: 28.0±5.3...

  2. Poor glycaemic control in Brazilian patients with type 2 diabetes attending the public healthcare system : a cross-sectional study

    Viana, Luciana Verçoza; Leitão, Cristiane Bauermann; Kramer, Caroline Kaercher; Zucatti, Alessandra Teixeira Netto; Jezini, Deborah Laredo; Felicio, João Soares; Mendes, Ana Beatriz Valverde; Chacra, Antonio Roberto; Azevedo, Mirela Jobim de; Gross, Jorge Luiz
    Objectives: To describe the clinical profile of Brazilian patients with type 2 diabetes attending the public healthcare system and identify factors associated with poor glycaemic control. Design: Cross-sectional study. Setting: 14 centres in five regions of Brazil, including primary care units and outpatient clinics of University Hospitals. Participants: Patients with type 2 diabetes attending outpatient clinics of public healthcare system. Main outcome measured: Glycated haemoglobin (HbA1c), centrally measured by high-performance liquid chromatography (National Glycohemoglobin Standardization Program certified). Results: A total of 5750 patients aged 61±10 years, with 11±8 years of diabetes duration (66% women, 56% nonwhite, body mass index: 28.0±5.3...

  3. Evaluation of different procedure involved in the Transcranial Direct Current Stimulation (tDCS) technique experimental application

    Adachi, Lauren Naomi Spezia; Oliveira, Carla de; Vercelino, Rafael; Macedo, Isabel Cristina de; Laste, Gabriela; Quevedo, Alexandre Silva de; Scarabelot, Vanessa Leal; Caumo, Wolnei; Torres, Iraci Lucena da Silva
    Introduction: The transcranial direct current stimulation (tDCS) is a non-invasive technique, which induces neuroplastic changes in the central nervous system of animals and humans. Furthermore, tDCS has been suggested as a therapeutic tool for pain management. The aim of this study was to standardize a non-invasive tDCS technique indexed by the nociceptive response of rats submitted to different conditions necessary to the tDCS application. Method: 60-day-old male Wistar rats (n=65), divided into 6 groups: control(C); non‑active sham (NAS); active-sham (AS); active-sham restrained (ASR); non-active sham restrained (NASR); active tDCS treatment. Animals received treatment during 30 seconds (sham-active) or 20 minutes...

  4. Evaluation of different procedure involved in the Transcranial Direct Current Stimulation (tDCS) technique experimental application

    Adachi, Lauren Naomi Spezia; Oliveira, Carla de; Vercelino, Rafael; Macedo, Isabel Cristina de; Laste, Gabriela; Quevedo, Alexandre Silva de; Scarabelot, Vanessa Leal; Caumo, Wolnei; Torres, Iraci Lucena da Silva
    Introduction: The transcranial direct current stimulation (tDCS) is a non-invasive technique, which induces neuroplastic changes in the central nervous system of animals and humans. Furthermore, tDCS has been suggested as a therapeutic tool for pain management. The aim of this study was to standardize a non-invasive tDCS technique indexed by the nociceptive response of rats submitted to different conditions necessary to the tDCS application. Method: 60-day-old male Wistar rats (n=65), divided into 6 groups: control(C); non‑active sham (NAS); active-sham (AS); active-sham restrained (ASR); non-active sham restrained (NASR); active tDCS treatment. Animals received treatment during 30 seconds (sham-active) or 20 minutes...

  5. New biotechnological tools to accelerate scab-resistance trait transfer to apple

    Cusin, Roberta; Revers, Luis Fernando; Maraschin, Felipe dos Santos
    Apple is a fruit crop cultivated worldwide. Apple orchards are exposed to a diverse set of environmental and biological factors that affect the productivity and sustainability of the culture. Many of the efforts and costs for apple production rely on reducing the incidence of fungal diseases, and one of the main diseases is apple scab caused by the fungus Venturia inaequalis. The economic impact of scab on apple productivity has guided many breeding programs to search for cultivars resistant to apple scab. Introgression from wild relatives has been successful to some extent, and genetic engineering for resistant cultivars has even...

  6. Interactions between plant hormones and heavy metals responses

    Bücker Neto, Lauro; Paiva, Ana Luiza Sobral; Machado, Ronei Dorneles; Arenhart, Rafael Augusto; Pinheiro, Marcia Margis
    Heavy metals are natural non-biodegradable constituents of the Earth’s crust that accumulate and persist indefinitely in the ecosystem as a result of human activities. Since the industrial revolution, the concentration of cadmium, arsenic, lead, mercury and zinc, amongst others, have increasingly contaminated soil and water resources, leading to significant yield losses in plants. These issues have become an important concern of scientific interest. Understanding the molecular and physiological responses of plants to heavy metal stress is critical in order to maximize their productivity. Recent research has extended our view of how plant hormones can regulate and integrate growth responses to...

  7. Salt stress affects mRNA editing in soybean chloroplasts

    Rodrigues, Nureyev Ferreira; Fonseca, Guilherme Cordenonsi da; Kulcheski, Franceli Rodrigues; Margis, Rogerio
    Soybean, a crop known by its economic and nutritional importance, has been the subject of several studies that assess the impact and the effective plant responses to abiotic stresses. Salt stress is one of the main environmental stresses and negatively impacts crop growth and yield. In this work, the RNA editing process in the chloroplast of soybean plants was evaluated in response to a salt stress. Bioinformatics approach using sRNA and mRNA libraries were employed to detect specific sites showing differences in editing efficiency. RT-qPCR was used to measure editing efficiency at selected sites. We observed that transcripts of NDHA,...

  8. Using ancient DNA to unravel taxonomic puzzles : the identity of Deuterodon pedri (Ostariophysi: Characidae)

    Silva, Priscilla Caroline; Malabarba, Maria Claudia de Souza Lima; Malabarba, Luiz Roberto
    Accurate identification is essential for any study exploring biodiversity. Unfortunately, museum type specimens preserved for more than a hundred years are often not informative enough for precise identification of the species represented by the name-bearing type. The use of ancient DNA can help solve taxonomic problems when name-bearing types no longer have diagnostic morphological features that allow for an accurate identification of the species involved. That is the case for Deuterodon pedri, an endemic species from a small drainage in the rio Doce basin in Minas Gerais, Brazil, for which the type material is in poor condition. Specimens of D....

  9. Effect of soybean ureases on seed germination and plant development

    Rechenmacher, Ciliana; Strohm, Beatriz Wiebke; Busatto, Luisa A. de Oliveira; Polacco, Joseph Carmine; Carlini, Celia Regina Ribeiro da Silva; Bodanese-Zanettini, Maria Helena
    Urease catalyzes the hydrolysis of urea to ammonia and carbon dioxide. The ammonia (nitrogen (N) product of urease activity) is incorporated into organic compounds. Thus, urease is involved in N remobilization, as well as in primary N assimilation. Two urease isoforms have been described for soybean: the embryo-specific, encoded by the Eu1 gene, and the ubiquitous urease, encoded by Eu4. A third urease-encoding gene was recently identified, designated Eu5, which encodes the putative protein product SBU-III. The present study aimed to evaluate the contribution of soybean ureases to seed germination and plant development. Analyses were performed using Eu1/Eu4/Eu5- co-suppressed transgenic...

  10. URS Campestre seedless orange : a new mutant with female sterility

    Bender, Renar João; Santos, Rinaldo Pires dos; Guerra, Divanilde; Schwarz, Sergio Francisco; Bender, Stefan da Silveira
    Seedlessness in fruit is a trait that is much sought after by juice making industries. Close to the city of São Sebastião do Caí, in the state of Rio Grande do Sul (RS), Brazil, a new mutant orange originating from natural mutation was identified and selected as a seedless material. To determine the mechanisms involved in the absence of seeds, the reproductive structures of this new mutant by comparison with a Valencia sweet orange as control, a cultivar with a profusion of seeds, was analyzed in terms of meiotic behavior, meiotic index, pollen viability, in vitro germination, and ovule features...

  11. Ontogenetic changes in mouth morphology triggers conflicting hypotheses of relationships in characid fishes (Ostariophysi: Characiformes)

    Hirschmann, Alice; Fagundes, Nelson Jurandi Rosa; Malabarba, Luiz Roberto
    Bryconamericus lethostigmus is the type-species of the monotypic genus Odontostoechus, diagnosed in part based on the presence of a unique tooth series in the premaxilla. Recently a new proposal of classification of the Stevardiinae placed Odontostoechus as a junior synonym of a monophyletic genus Bryconamericus sensu stricto, a genus characterized by the presence of two tooth series. Bryconamericus lethostigmus is redescribed herein and the single tooth series in the premaxilla is demonstrated to originate from merging of the external tooth row with the inner row during ontogeny refuting primary hypothesis of homology between the mouth morphology of B. lethostigmus and...

  12. Secondary structure of nrDNA Internal Transcribed Spacers as a useful tool to align highly divergent species in phylogenetic studies

    Giudicelli, Giovanna Câmara; Mader, Geraldo; Arias, Gustavo Adolfo Silva; Zamberlan, Priscilla Mena; Bonatto, Sandro Luis; Freitas, Loreta Brandao de
    Recently, it has been suggested that internal transcribed spacer (ITS) sequences are under selective constraints to preserve their secondary structure. Here, we investigate the patterns of the ITS nucleotide and secondary structure conservation across the Passiflora L. genus to evaluate the potential use of secondary structure data as a helpful tool for the alignment in taxonomically complex genera. Considering the frequent use of ITS, this study also presents a perspective on future analyses in other plant groups. The ITS1 and ITS2 sequences presented significant differences for mean values of the lowest energy state (LES) and for number of hairpins in...

  13. Secondary structure of nrDNA Internal Transcribed Spacers as a useful tool to align highly divergent species in phylogenetic studies

    Giudicelli, Giovanna Câmara; Mader, Geraldo; Arias, Gustavo Adolfo Silva; Zamberlan, Priscilla Mena; Bonatto, Sandro Luis; Freitas, Loreta Brandao de
    Recently, it has been suggested that internal transcribed spacer (ITS) sequences are under selective constraints to preserve their secondary structure. Here, we investigate the patterns of the ITS nucleotide and secondary structure conservation across the Passiflora L. genus to evaluate the potential use of secondary structure data as a helpful tool for the alignment in taxonomically complex genera. Considering the frequent use of ITS, this study also presents a perspective on future analyses in other plant groups. The ITS1 and ITS2 sequences presented significant differences for mean values of the lowest energy state (LES) and for number of hairpins in...

  14. High-Frequency Sequences in the Quaternary of Pelotas Basin (coastal plain): a record of degradational stacking as a function of longer-term base-level fall

    Rosa, Maria Luiza Correa da Camara; Barboza, Eduardo Guimaraes; Abreu, Vitor dos Santos; Tomazelli, Luiz Jose; Dillenburg, Sergio Rebello
    The aim of this work was to analyze the sedimentary record of the coastal plain of Pelotas Basin, placing it in the context of temporal and spatial scales, and proposing a stratigraphic hierarchical framework. The coastal plain, located in southern Brazil and in northern Uruguay, is mainly formed by four Quaternary barrier-lagoon systems. Three of these systems were studied through the integration of surface and subsurface data (geomorphological and topographical mapping, outcrops description, geoprocessing, dating and Ground Penetrating Radar — GPR — records) and interpreted as the preserved, onshore portion of depositional sequences mainly controlled by glacioeustatic cycles of about...

  15. Taxonomy and evolutionary relationships of Passiflora subg. Decaloba supersect. Decaloba sect. Xerogona (Passifloraceae) : contributions of palynological, morphological and molecular studies

    Azevedo, Michaele Alvim Milward de; Freitas, Loreta Brandao de; Kinoshita, Luiza Sumiko
    Passiflora subg. Decaloba supersect. Decaloba sect. Xerogona (Passifloraceae) is a tropical and subtropical group comprising 14 species that occur in tropical biomes throughout Latin America, including the Atlantic Forest. The section Xerogona comprises herbaceous vines characterized by a lack of petiole glands on their leaves, of bracts and of ocelli on their leaf blades, as well as by their capsular fruits. We analyzed the phylogeny on the basis of morphological characters (including pollen characters) and molecular data. The inferred phylogeny was used in order to characterize, circumscribe and delimit the section and the species. We examined the phylogenetic relationships among...

  16. New microdeletion and microduplication syndromes : a comprehensive review

    Nevado Blanco, Julián; Mergener, Rafaella; Palomares-Bralo, María; Souza, Karen Regina Silva de; Vallespín García, Elena; Mena, Rocío; Martínez-Glez, Víctor; Palomino, María Ángeles; Santos Simarro, Fernando; García Miñaur, Sixto; García Santiago, Fé Amalia; Mansilla Aparicio, Elena; Fernández García-Moya, Luis; Torres Pérez Hidalgo, María Luisa de; Mariluce Riegel, Mariluce Riegel; Lapunzina Badía, Pablo D.
    Several new microdeletion and microduplication syndromes are emerging as disorders that have been proven to cause multisystem pathologies frequently associated with intellectual disability (ID), multiple congenital anomalies (MCA), autistic spectrum disorders (ASD) and other phenotypic findings. In this paper, we review the “new” and emergent microdeletion and microduplication syndromes that have been described and recognized in recent years with the aim of summarizing their main characteristics and chromosomal regions involved. We decided to group them by genomic region and within these groupings have classified them into those that include ID, MCA, ASD or other findings. This review does not intend...

  17. New microdeletion and microduplication syndromes : a comprehensive review

    Nevado Blanco, Julián; Mergener, Rafaella; Palomares-Bralo, María; Souza, Karen Regina Silva de; Vallespín García, Elena; Mena, Rocío; Martínez-Glez, Víctor; Palomino, María Ángeles; Santos Simarro, Fernando; García Miñaur, Sixto; García Santiago, Fé Amalia; Mansilla Aparicio, Elena; Fernández García-Moya, Luis; Torres Pérez Hidalgo, María Luisa de; Mariluce Riegel, Mariluce Riegel; Lapunzina Badía, Pablo D.
    Several new microdeletion and microduplication syndromes are emerging as disorders that have been proven to cause multisystem pathologies frequently associated with intellectual disability (ID), multiple congenital anomalies (MCA), autistic spectrum disorders (ASD) and other phenotypic findings. In this paper, we review the “new” and emergent microdeletion and microduplication syndromes that have been described and recognized in recent years with the aim of summarizing their main characteristics and chromosomal regions involved. We decided to group them by genomic region and within these groupings have classified them into those that include ID, MCA, ASD or other findings. This review does not intend...

  18. Gm and Inv allotypes of brazilian Cayapo indians

    Salzano, Francisco Mauro; Steinberg, A. G.; Tepfenhart, Mary A.
    Data from 440 individuals belonging to four populations of Brazilian Cayapo Indians are reported. About 99% of them are Gm (1, 21) or Gm (1, 2, 21), while the frequency of sera with Inv (1) varies from 59%7 to 75%. These values are generally in accordance with those previously obtained among other South American Indians. The four populations can be separated into two groups if the distribution of Gm' 21 and Gm 2, 21 is considered, but this distinction is not apparent in the frequencies of Inv'. A surprisingly high number of samples showed antibody activity against the sensitized red...

  19. Gm and Inv allotypes of brazilian Cayapo indians

    Salzano, Francisco Mauro; Steinberg, A. G.; Tepfenhart, Mary A.
    Data from 440 individuals belonging to four populations of Brazilian Cayapo Indians are reported. About 99% of them are Gm (1, 21) or Gm (1, 2, 21), while the frequency of sera with Inv (1) varies from 59%7 to 75%. These values are generally in accordance with those previously obtained among other South American Indians. The four populations can be separated into two groups if the distribution of Gm' 21 and Gm 2, 21 is considered, but this distinction is not apparent in the frequencies of Inv'. A surprisingly high number of samples showed antibody activity against the sensitized red...

  20. Effect of nonsurgical periodontal therapy on serum and gingival crevicular fluid cytokine levels during pregnancy and postpartum

    Fiorini, Tiago; Susin, Cristiano; Rocha, José Mariano da; Weidlich, Patrícia; Vianna, Priscila; Moreira, Carlos Heitor Cunha; Chies, Jose Artur Bogo; Rösing, Cassiano Kuchenbecker; Oppermann, Rui Vicente
    Background and Objective: A low-grade systemic inflammatory status originating from periodontal infection has been proposed to explain the association between periodontal disease and systemic conditions, including adverse obstetric outcomes. The aim of this study was to evaluate the effect of periodontal therapy during pregnancy on the gingival crevicular fluid and serum levels of six cytokines associated with periodontal disease and preterm birth. Material and Methods: A subsample of 60 women (18–35 years of age) up to 20 gestational weeks, previously enrolled in a larger randomized clinical trial, was recruited for the present study. Participants were randomly allocated to receive either...

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