Mostrando recursos 1 - 20 de 250

  1. Comunicação científica em livros e capítulos na área de Educação e afins

    Ferreira, Ana Gabriela Clipes; Wannmacher, Clovis Milton Duval
    Este artigo tem como objetivo analisar as publicações de uma Faculdade de Educação brasileira em livros e capítulos de livros. Contextualiza a comunidade estudada e o veículo de comunicação científica formal livro, bem como faz breve discussão sobre o comportamento de publicação da área da educação e áreas afins. Pesquisa cientométrica, de caráter exploratório e descritivo. Com recorte temporal de 2000 a 2015, os livros e capítulos representaram um total de 2740 registros, ou 17,97% do total da produção. Quando verificados os itens com maior número de circulação na biblioteca, verifica-se que existe ligação entre estes e a produção docente...

  2. Quality of life in mucopolysaccharidoses : construction of a specific measure using the focus group technique

    Oliveira, Michele Rosana; Schwartz, Ida Vanessa Doederlein; Costa, Liciane da Silva; Maia Filho, Heber de Souza; Ribeiro, Márcia Gonçalves; Guerreiro, L. B.; Acosta, Angelina Xavier; Rocha, Neusa Sica da
    Abstract Objective: To describe the perceptions of patients, their caregivers, and their healthcare providers to the development of a new specific instrument for assessment of the quality of life (QoL) in patients with mucopolysaccharidoses (MPS) using a qualitative focus group (FG) design. FGs were held in two Brazilian states (Rio Grande do Sul and Rio de Janeiro). Results: Three versions of the new instrument were developed, each for a different age group: children (age 8–12 years), adolescents (age 13–17), and adults (age ≥ 18). The FGs mostly confirmed the relevance of items. All FGs unanimously agreed on the facets: School,...

  3. Cytological and genome size data analyzed in a phylogenetic frame : evolutionary implications concerning Sisyrinchium taxa (Iridaceae: Iridoideae)

    Piccoli, Paula Burchardt; Chies, Tatiana Teixeira de Souza; Chauveau, Olivier; Callegari-Jacques, Sidia Maria; Corrêa, Lauís Brisolara; Inácio, Camila Dellanhese; Eggers, Lilian; Siljak-Yakovlev, Sonja; Campos, José Marcello Salabert; Santos, Eliane Kaltchuk dos
    Sisyrinchium is the largest genus of Iridaceae in the Americas and has the greatest amount of cytological data available. This study aimed at investigating how genomes evolved in this genus. Chromosome number, genome size and altitude from species of sect. Viperella were analyzed in a phylogenetic context. Meiotic and pollen analyses were performed to assess reproductive success of natural populations, especially from those polyploid taxa. Character optimizations revealed that the common ancestor of sect. Viperella was probably diploid (2n = 2x =18) with two subsequent polyplodization events. Total DNA content (2C) varied considerably across the phylogeny with larger genomes detected...

  4. Demography and genetics at the tribal level : the Xavante as a test case

    Callegari-Jacques, Sidia Maria; Flowers, Nancy M.; Laner, Nara F. M.; Salzano, Francisco Mauro
    Resumo não disponível

  5. Effect of sciatic nerve transection on acetylcholinesterase activity in spinal cord and skeletal muscles of the bullfrog Lithobates catesbeianus

    Kroth, Adarly; Mackedanz, Vanize; Matté, Cristiane; Wyse, Angela Terezinha de Souza; Ribeiro, Maria Flavia Marques; Partata, Wania Aparecida
    Sciatic nerve transection (SNT), a model for studying neuropathic pain, mimics the clinical symptoms of “phantom limb”, a pain condition that arises in humans after amputation or transverse spinal lesions. In some vertebrate tissues, this condition decreases acetylcholinesterase (AChE) activity, the enzyme responsible for fast hydrolysis of released acetylcholine in cholinergic synapses. In spinal cord of frog Rana pipiens, this enzyme’s activity was not significantly changed in the first days following ventral root transection, another model for studying neuropathic pain. An answerable question is whether SNT decreases AChE activity in spinal cord of frog Lithobates catesbeianus, a species that has...

  6. Resveratrol prevents cellular and behavioral sensory alterations in the animal model of autism induced by valproic Acid

    Silva, Mellanie Fontes Dutra da; Machado, Júlio Santos Terra; Deckmann, Iohanna; Schwingel, Gustavo Brum; Nunes, Gustavo Della Flora; Hirsch, Mauro Mozael; Negrini, Guilherme Bauer; Riesgo, Rudimar dos Santos; Bambini Júnior, Victorio; Hedin Pereira, Cecília; Gottfried, Carmem Juracy Silveira
    Autism spectrum disorder (ASD) is characterized by impairments in both social communication and interaction and repetitive or stereotyped behaviors. Although its etiology remains unknown, genetic and environmental risk factors have been associated with this disorder, including the exposure to valproic acid (VPA) during pregnancy. Resveratrol (RSV) is an anti-inflammatory and antioxidant molecule known to prevent social impairments in the VPA animal model of autism. This study aimed to analyze the effects of prenatal exposure to VPA, as well as possible preventive effects of RSV, on sensory behavior, the localization of GABAergic parvalbumin (PVC) neurons in sensory brain regions and the...

  7. Absence of A3Z3-related hypermutations in the env and vif proviral genes in FIV naturally infected cats

    Cano Ortiz, Lucía; Junqueira, Dennis Maletich; Comerlato, Juliana; Zani, André Luis da Silva; Costa, Cristina Santos da; Roehe, Paulo Michel; Franco, Ana Claudia
    Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3 (APOBEC3; A3) proteins comprise an important family of restriction factors that produce hypermutations on proviral DNA and are able to limit virus replication. Vif, an accessory protein present in almost all lentiviruses, counteracts the antiviral A3 activity. Seven haplotypes of APOBEC3Z3 (A3Z3) were described in domestic cats (hap I–VII), and in-vitro studies have demonstrated that these proteins reduce infectivity of vif-defective feline immunodeficiency virus (FIV). Moreover, hap V is resistant to vif-mediated degradation. However, studies on the effect of A3Z3 in FIV-infected cats have not been developed. Here, the correlation between APOBEC A3Z3...

  8. Isoflurane and the analgesic effect of acupuncture and electroacupuncture in an animal model of neuropathic pain

    Adachi, Lauren Naomi Spezia; Vercelino, Rafael; Oliveira, Carla de; Scarabelot, Vanessa Leal; Souza, Andressa de; Medeiros, Liciane Fernandes; Cioato, Stefania Giotti; Caumo, Wolnei; Torres, Iraci Lucena da Silva
    The present study aimed to determine whether isoflurane interferes with the analgesic effects of acupuncture (Ac) and electroacupuncture (EA), using a neuropathic pain (NP) rat model. In total, 140 male Wistar rats were used; isoflurane-induced nociceptive response was evaluated using the von Frey test, serum calcium-binding protein b (S100b) levels and nerve growth factor (NGF) levels in the left sciatic nerve. The NP model was induced by chronic constriction injury of the sciatic nerve at 14 days after surgery. Treatment was initiated after NP induction with or without isoflurane anesthesia (20 min/ day/8 days). The von Frey test was performed...

  9. Apa-I polymorphism in VDR gene is related to metabolic syndrome in polycystic ovary syndrome : a cross-sectional study

    Santos, Betânia Rodrigues dos; Lecke, Sheila Bünecker; Spritzer, Poli Mara
    Background: Polycystic ovary syndrome (PCOS) is a common endocrine disorder determined by polygenic traits as well as environmental factors. Lower vitamin D levels have been detected in PCOS women and related to hormone and metabolic disturbances. Vitamin D acts in tissues through the vitamin D receptor (VDR). VDR gene variants have been associated with worse metabolic profile in the general population. We investigated the genotype and haplotype distribution of the Bsm-I (rs1544410), Apa-I (rs7975232), and Taq-I (rs731236) VDR gene polymorphisms in PCOS and non-hirsute women from southern Brazil. We further investigated the associations of these gene variants and their haplotypes...

  10. Data on social transmission of food preference in a model of autism induced by valproic acid and translational analysis of circulating microRNA

    Hirsch, Mauro Mozael; Deckmann, Iohanna; Silva, Mellanie Fontes Dutra da; Negrini, Guilherme Bauer; Nunes, Gustavo Della Flora; Nunes, Walquiria Souza; Rabelo, Bruna Daniel; Riesgo, Rudimar dos Santos; Margis, Rogerio; Bambini Júnior, Victorio; Gottfried, Carmem Juracy Silveira
    This article contains data of Social Transmission of Food Preference in an animal model of autism and the evaluation of a set of microRNA analyzed in autistic patients and animal model of autism. The analyses of the absolute consumption of two flavored food by male rats prenatally exposed to valproic acid (VPA) and treated with resveratrol (RSV), showed that VPA animals show a trend to eat less of the flavored food presented by a demonstrator rat. We also identified 13 microRNA with similar levels among rodents’ experimental groups, as well as 11 microRNA with no alterations between autistic and control...

  11. The oleaginous yeast Meyerozyma guilliermondii BI281A as a new potential biodiesel feedstock : selection and lipid production optimization

    Ramírez Castrillón, Mauricio; Garcia, Victoria Patricia Jaramillo; Rosa, Priscila Dallé da; Landell, Melissa Fontes; Vu, Duong; Fabricio, Mariana Fensterseifer; Ayub, Marco Antônio Záchia; Robert, Vicent; Henriques, Joao Antonio Pegas; Silva, Patrícia Valente da
    A high throughput screening (HTS) methodology for evaluation of cellular lipid content based on Nile red fluorescence reads using black background 96-wells test plates and a plate reader equipment allowed the rapid intracellular lipid estimation of strains from a Brazilian phylloplane yeast collection. A new oleaginous yeast,Meyerozyma guilliermondii BI281A, was selected, for which the gravimetric determination of total lipids relative to dry weight was 52.38%for glucose or 34.97%for pure glycerol. The lipid production was optimized obtaining 108 mg/L of neutral lipids using pure glycerol as carbon source, and the strain proved capable of accumulating oil using raw glycerol from a...

  12. Cofilin-1 levels and intracellular localization are associated with melanoma prognosis in a cohort of patients

    Bracalente, Candelaria; Rinflerch, Adriana; Ibañez, Irene Laura; Garcia, Francisco M.; Volonteri, Victoria; Galimberti, Gastón N.; Klamt, Fabio; Durán, Hebe
    Melanoma is an aggressive cancer with highly metastatic ability. We propose cofilin-1, a key protein in the regulation of actin dynamics and migration, as a prognostic marker. We determined cofilin-1 levels in a retrospective cohort of patients with melanomas and benign lesions of melanocytes (nevi) by immunohistochemistry. Higher cofilin-1 levels were found in malignant melanoma (MM) with Breslow Index (BI)>2 vs MM with BI<2, melanoma in situ (MIS) and nevi and also in MM with metastasis vs MM without detected metastasis. Kaplan-Meier survival curves were performed, clustering patients according to either the type of melanocytic lesions or cofilin-1 level. Survival...

  13. Effects of Achyrocline satureioides inflorescence extracts against pathogenic intestinal bacteria : chemical characterization, in vitro tests, and in vivo evaluation

    Moresco, Karla Suzana; Silveira, Alexandre Kleber; Zeidán-Chuliá, Fares; Correa, Ana Paula Folmer; Oliveira, Rafael Rodrigues de; Borges, Adriana Giongo; Grun, Lucas Kich; Barbé-Tuana, Florencia María; Zmozinski, Ariane Vanessa; Brandelli, Adriano; Vale, Maria Goreti Rodrigues; Gelain, Daniel Pens; Bassani, Valquiria Linck; Moreira, Jose Claudio Fonseca
    Three Achyrocline satureioides (AS) inflorescences extracts were characterized: (i) a freeze-dried extract prepared fromthe aqueous extractive solution and (ii) a freeze-dried and (iii) a spray-dried extract prepared from hydroethanol extractive solution (80% ethanol).The chemical profile, antioxidant potential, and antimicrobial activity against intestinal pathogenic bacteria of AS extracts were evaluated. In vitro antioxidant activity was determined by the total reactive antioxidant potential (TRAP) assay. In vivo analysis and characterization of intestinal microbiota were performed in male Wistar rats (saline versus treated animals with AS dried extracts) by high-throughput sequencing analysis: metabarcoding. Antimicrobial activity was tested in vitro by the disc diffusion...

  14. Effects of methanol fraction from leaves of Schinus terebinthifolius raddi on nociception and spinal-cord oxidative biomarkers in rats with neuropathic pain

    Scheid, Taina; Moraes, Maira Silmara de; Henriques, Thiago Pereira; Riffel, Ana Paula Konzen; Belló-Klein, Adriane; Von Poser, Gilsane Lino; Ethur, Eduardo Miranda; Partata, Wania Aparecida
    We determined the antioxidant potential of fractions obtained from leaves of Schinus terebinthifolius, a medicinal plant known in Brazil as aroeira, to select the fraction with the best yield and antioxidant performance. These qualities were found in themethanol fraction (MeF), which was administered intraperitoneally (20mg/kg/day) for 3 and 10 days to rats with chronic constriction injury (CCI) of the sciatic nerve, a model of neuropathic pain. The MeF increased the mechanical and thermal thresholds that had been lowered by CCI. In parallel, the lumbosacral spinal cord showed an increase in superoxide dismutase but a decrease in glutathione peroxidase and glutathione-S-transferase...

  15. The impact of deep brain stimulation on the quality of life and swallowing in individuals with Parkinson's Disease

    Olchik, Maira Rozenfeld; Ghisi, Marciele; Ayres, Annelise; Schuh, Artur Francisco Schumacher; Oppitz, Paulo Petry; Rieder, Carlos Roberto de Mello
    Introduction Deep brain stimulation (DBS) is an established treatment for Parkinson’s disease (PD). However, there is little evidence regarding the effect of DBS on dysphagia. Objective To assess the swallowing and quality of life of individuals with PD before and after DBS surgery. Methods Our sample consisted of people who had undergone DBS surgery in a referral hospital in the state of Rio Grande do Sul, Brazil. The inclusion criteria were a diagnosis of PD and having undergone DBS surgery. A cognitive screening, through a questionnaire about depression and quality of life, was conducted. Evaluations of each patient’s swallowing were...

  16. Phylogenetic grouping based on triplex PCR of multiresistant Escherichia coli of environmental, human and animal origin

    Pastore, Ana Paula Winter; Canal, Natalia; Costa, Marisa da; Corção, Gertrudes
    (Grupamento filogenético baseado em PCR triplex de Escherichia coli multiresistentes de origem ambiental, humana e animal). A bactéria Escherichia coli é largamente utilizada como indicador biológico de contaminação fecal devido à sua ubiquidade em fezes. No entanto, possui também a capacidade de persistir e multiplicar em ambientes fora do seu habitat primário. A subestrutura genética de E. coli pode ser determinada através de presença/ausência dos genes chuA e yjaA e do fragmento de DNA TspE4.C2, com base nesta metodologia cepas podem ser classificadas como pertencentes aos filogrupos A, B1, B2 ou D. Este estudo teve como objetivo realizar a determinação...

  17. Treatment with ascorbic acid and α-tocopherol modulates oxidative-stress markers in the spinal cord of rats with neuropathic pain

    Riffel, Ana Paula Konzen; Santos, Maria do Carmo Quevedo; Souza, Jéssica Araújo de; Scheid, Taina; Horst, Andréa; Kolberg, Carolina; Belló-Klein, Adriane; Partata, Wania Aparecida
    Vitamin E (vit. E) and vitamin C (vit. C) are antioxidants that inhibit nociception. The effect of these vitamins on oxidative-stress markers in the spinal cord of rats with chronic constriction injury (CCI) of the sciatic nerve is unknown. This study investigated the effect of intraperitoneal administration of vit. E (15 mg kg–1 day–1) and vit. C (30 mg kg–1 day–1), given alone or in combination, on spinal cord oxidative-stress markers in CCI rats. Adult male Wistar rats weighing 200–250 g were divided equally into the following groups: Naive (rats did not undergo surgical manipulation); Sham (rats in which all...

  18. Preclinical to clinical translation of studies of transcranial direct-current stimulation in the treatment of epilepsy : a systematic review

    Regner, Gabriela Gregory; Pereira, Patricia; Leffa, Douglas Teixeira; Oliveira, Carla de; Vercelino, Rafael; Fregni, Felipe; Torres, Iraci Lucena da Silva
    Epilepsy is a chronic brain syndrome characterized by recurrent seizures resulting from excessive neuronal discharges. Despite the development of various new antiepileptic drugs, many patients are refractory to treatment and report side effects. Non-invasive methods of brain stimulation, such as transcranial direct current stimulation (tDCS), have been tested as alternative approaches to directly modulate the excitability of epileptogenic neural circuits. Although some pilot and initial clinical studies have shown positive results, there is still uncertainty regarding the next steps of investigation in this field. Therefore, we reviewed preclinical and clinical studies using the following framework: (1) preclinical studies that have...

  19. Psychotropic in the environment : risperidone residues affect the behavior of fish larvae

    Kalichak, Fabiana; Idalencio, Renan; Rosa, João Gabriel Santos; Barcellos, Heloisa Helena de Alcantara; Fagundes, Michele; Piato, Angelo Luis Stapassoli; Barcellos, Leonardo Jose Gil
    Resumo não disponível

  20. Network-based identification of altered stem cell pluripotency and calcium signaling pathways in metastatic melanoma

    Oliveira, Ben Hur Neves de; Dalmaz, Carla; Zeidán-Chuliá, Fares
    Malignancy of cancer has been linked to distinct subsets of stem-like cells, the so-called cancer stem cells (CSCs), which persist during treatment and seem to lead to drug-resistant recurrence. Metastatic spread of cancer cells is one of the hallmarks of malignancy and contributes to most human melanoma-related deaths. Recently, overlapping groups of proteins and pathways were shown to regulate stem cell migration and cancer metastasis, raising the question of whether genes/proteins involved in stem cell pluripotency may have important implications when applied to the biology of cancer metastasis. Furthermore, it is well known that ion channels and receptors, particularly those...

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