Mostrando recursos 1 - 20 de 199

  1. Geometrical distribution of Cryptococcus neoformans mediates flower-like biofilm development

    Lopes, William; Vainstein, Mendeli Henning; Araújo, Glauber Ribeiro de Sousa; Frases, Susana; Staats, Charley Christian; Almeida, Rita Maria Cunha de; Schrank, Augusto; Silva, Lívia Kmetzsch Rosa e; Vainstein, Marilene Henning
    Microbial biofilms are highly structured and dynamic communities in which phenotypic diversification allows microorganisms to adapt to different environments under distinct conditions. The environmentally ubiquitous pathogen Cryptococcus neoformans colonizes many niches of the human body and implanted medical devices in the form of biofilms, an important virulence factor. A new approach was used to characterize the underlying geometrical distribution of C. neoformans cells during the adhesion stage of biofilm formation. Geometrical aspects of adhered cells were calculated from the Delaunay triangulation and Voronoi diagramobtained fromscanning electronmicroscopy images (SEM). A correlation between increased biofilm formation and higher ordering of the underlying...

  2. Effects of n-3 fatty acids and exercise on oxidative stress parameters in type 2 diabetic : a randomized clinical trial

    Fayh, Ana Paula Trussardi; Sapata, Katiuce Borges; Cunha, Giovani dos Santos; Krause, Maurício; Rocha, Ricardo; Bittencourt Junior, Paulo Ivo Homem de; Moreira, Jose Claudio Fonseca; Friedman, Rogério; Rossato, Juliane da Silva; Fernandes, João Roberto; Oliveira, Álvaro Reischak de
    Background: The relationship between diabetes and oxidative stress has been previously reported. Exercise represents a useful non-pharmacological strategy for the treatment in type 2 diabetic (T2DM) patients, but high intensity exercise can induce a transient inflammatory state and increase oxidative stress. Nutritional strategies that may contribute to the reduction of oxidative stress induced by acute exercise are necessary. The aim of this study was to examine if n-3 PUFA supplementation intervention can attenuate the inflammatory response and oxidative stress associated with high intensity exercise in this population. As a primary outcome, lipoperoxidation measurements (TBARS and F2-isoprostanes) were selected. Methods: Thirty...

  3. Neuro-Behçet disease mimicking brain tumor : A case report

    Tramontini, Pedro Leonel; Finkelsztejn, Alessandro; Duarte, Juliana Ávila; Santos, Guilherme Tomasi; Roesler, Rafael; Isolan, Gustavo Rassier
    Background: Behçet’s disease (BD) is an inflammatory multisystem disease with unknown etiology, and consists of a TRIAD comprising recurrent oral ulcers, genital ulcers, and uveitis. In some cases, the disease affects the central nervous system, called Neuro‑Behçet Disease (NBD). Few cases of NBD simulating a brain tumor have been previously reported. Case Description: Here, we describe the case of a 46‑year‑old male patient with a previous diagnosis of brain tumor who was later diagnosed for BD. Conclusion: This case highlights the importance of differential diagnosis of lesions with tumoral features. Checking for the possibility of NBD may help avoiding biopsy...

  4. Liver glutathione depletion after preservation and reperfusion in human liver transplantation

    Grezzana Filho, Tomáz de Jesus Maria; Corso, Carlos Otavio; Zanotelli, Maria Lucia; Marroni, Claudio Augusto; Brandao, Ajacio Bandeira de Mello; Schlindwein, Eduardo S.; Leipnitz, Ian; Meine, Mario Henrique Mendes de Mattos; Fleck Júnior, Alfeu de Medeiros; Hoppen, Ricardo Antônio; Kiss, Guilhermo; Cantisani, Guido Pio Gracco
    Purpose: The oxidative stress is an important mechanism responsible for dysfunction after orthotopic liver transplantation (OLT). Glutathione (GSH) low levels after cold storage render the grafts vulnerable to reperfusion injury. Aim of this study was to evaluate GSH and oxidized glutathione (GSSG) liver concentrations, the hepatocellular injury and function in optimal and suboptimal grafts after human OLT. Methods: Liver biopsies were taken in 33 patients before the implant and two hours after reperfusion, allowing determination of GSH, GSSG and oxidative stress ratio (GSH/GSSG). Serum transaminases, prothrombin activity (PT) and factor V were measured to evaluate injury and function respectively. Histopathological...

  5. Hypertonic saline and hemorrhagic shock : hepatocellular function and integrity after six hours of treatment

    Hoppen, Ricardo Antônio; Corso, Carlos Otavio; Grezzana Filho, Tomáz de Jesus Maria; Severino, Antonio Generoso; Dal Pizzol, Felipe; Ritter, Cristiane
    Purpose: The comparison after 6h of hemorrhagic shock (HS) treatment with NaCl 7.5% (Hypertonic Saline Solution – SSH) or Ringer Lactate (RL) on liver function and integrity. Methods: Male Wistar rats were submitted to HS (Mean Arterial Pressure – MAP= 45 mmHg) during 60 min and then treated with NaCl 7.5% (SSH, 10% of blood loss, n=8) or Ringer Lactate (RL, 400% of blood loss, n=8). After 6h rats were anesthetized, hepatic function was assessed by bile flow measurement and liver integrity evaluated by determination of alanine aminotransferase (ALT) and bilirubin activities. Results: There was no difference in MAP between...

  6. Peripheral inflammatory markers contributing to comorbidities in autism

    Jácome, Martha Cecilia Inga; Chacòn, Lilia Maria Morales; Cuesta, Hector Vera; Rizo, Carlos Maragoto; Santiesteban, Mabel Whilby; Hernandez, Lesyanis Ramos; García, Elena Noris; Fraguela, Maria Elena González; Verdecia, Caridad Ivette Fernandez; Hurtado, Yamilé Vegas; Siniscalco, Dario; Goncalves, Carlos Alberto Saraiva; Robinson Agramonte, María de los Ángeles
    This study evaluates the contribution of peripheral biomarkers to comorbidities and clinical findings in autism. Seventeen autistic children and age-matched typically developing (AMTD), between three to nine years old were evaluated. The diagnostic followed the Diagnostic and Statistical Manual of Mental Disorders 4th Edition (DMS-IV) and the Childhood Autism Rating Scale (CARS) was applied to classify the severity. Cytokine profile was evaluated in plasma using a sandwich type ELISA. Paraclinical events included electroencephalography (EEG) record. Statistical analysis was done to explore significant differences in cytokine profile between autism and AMTD groups and respect clinical and paraclinical parameters. Significant differences were...

  7. Differential expression of transcriptional regulatory units in the prefrontal cortex of patients with bipolar disorder : potential role of early growth response gene 3

    Pfaffenseller, Bianca; Magalhães, Pedro Vieira da Silva; Bastiani, Marco Antônio de; Castro, Mauro Antônio Alves; Gallitano, Amelia L.; Kapczinski, Flávio Pereira; Klamt, Fabio
    Bipolar disorder (BD) is a severe mental illness with a strong genetic component. Despite its high degree of heritability, current genetic studies have failed to reveal individual loci of large effect size. In lieu of focusing on individual genes, we investigated regulatory units (regulons) in BD to identify candidate transcription factors (TFs) that regulate large groups of differentially expressed genes. Network-based approaches should elucidate the molecular pathways governing the pathophysiology of BD and reveal targets for potential therapeutic intervention. The data from a large-scale microarray study was used to reconstruct the transcriptional associations in the human prefrontal cortex, and results...

  8. Cell therapy in the treatment of bipolar mania in an animal model : a proof of concept study

    Ascoli, Bruna Maria; Colombo, Rafael; Géa, Luíza Paul; Terraciano, Paula Barros; Pizzato, Sabrina Beal; Oliveira, Fernanda Santos de; Cirne Lima, Elizabeth Obino; Kapczinski, Flávio Pereira; Rosa, Adriane Ribeiro
    Introduction: The rationale of mesenchymal stem cells (MSCs) as a novel therapeutic approach in certain neurodegenerative diseases is based on their ability to promote neurogenesis. Hippocampal atrophy has been related to bipolar disorder (BD) in preclinical, imaging and postmortem studies. Therefore, the development of new strategies to stimulate the neurogenesis process in BD is crucial. Objectives: To investigate the behavioral and neurochemical changes induced by transplantation of MSCs in a model of manialike behavior induced by lisdexamfetamine dimesylate (LDX). Methods: Wistar rats (n=65) received one oral daily dose of LDX (10 mg/kg) or saline for 14 days. On the 8th...

  9. Membrane particles generated from mesenchymal stromal cells modulate immune responses by selective targeting of pro-inflammatory monocytes

    Gonçalves, Fabiany da Costa; Luk, Franka; Korevaar, Sander S.; Bouzid, Rachid; Paz, Ana Helena da Rosa; Iglesias, Carmen López; Baan, Carla C.; Merino, Ana; Hoogduijn, Martin J.
    Mesenchymal stromal cells (MSC) are a promising therapy for immunological disorders. However, culture expanded MSC are large and get trapped in the capillary networks of the lungs after intravenous infusion, where they have a short survival time. Hypothetically, living cells are a risk for tumor formation. To reduce risks associated with MSC infusion and improve the distribution in the body, we generated membrane particles (MP) of MSC and MSC stimulated with IFN-γ (MPγ). Tracking analysis and electron microscopy indicated that the average size of MP was 120 nm, and they showed a round shape. MP exhibited ATPase, nucleotidase and esterase...

  10. Genome characterization of the first mimiviruses of lineage C isolated in Brazil

    Assis, Felipe Lopes de; Franco-Luiz, Ana Paula Moreira; Santos, Raíssa Nunes dos; Campos, Fabrício Souza; Dornas, Fábio Pio; Borato, Paulo V. M.; Franco, Ana Claudia; Abrahão, Jônatas Santos; Colson, Philippe; Scola, Bernard La
    The family Mimiviridae, comprised by giant DNA viruses, has been increasingly studied since the isolation of the Acanthamoeba polyphaga mimivirus (APMV), in 2003. In this work, we describe the genome analysis of two new mimiviruses, each isolated from a distinct Brazilian environment. Furthermore, for the first time, we are reporting the genomic characterization of mimiviruses of group C in Brazil (Br-mimiC), where a predominance of mimiviruses from group A has been previously reported. The genomes of the Br-mimiC isolates Mimivirus gilmour (MVGM) and Mimivirus golden (MVGD) are composed of double-stranded DNA molecules of 1.2 Mb, each encoding more than 1,100...

  11. Reprogramming human A375 amelanotic melanoma cells by catalase overexpression : Upregulation of antioxidant genes correlates with regression of melanoma malignancy and with malignant progression when downregulated

    Bracalente, Candelaria; Ibañez, Irene Laura; Berenstein, Ariel; Notcovich, Cintia; Cerda, María B.; Klamt, Fabio; Chernomoretz, Ariel; Durán, Hebe
    Reactive oxygen species (ROS) are implicated in tumor transformation. The antioxidant system (AOS) protects cells from ROS damage. However, it is also hijacked by cancers cells to proliferate within the tumor. Thus, identifying proteins altered by redox imbalance in cancer cells is an attractive prognostic and therapeutic tool. Gene expression microarrays in A375 melanoma cells with different ROS levels after overexpressing catalase were performed. Dissimilar phenotypes by differential compensation to hydrogen peroxide scavenging were generated. The melanotic A375-A7 (A7) upregulated TYRP1, CNTN1 and UCHL1 promoting melanogenesis. The metastatic A375-G10 (G10) downregulated MTSS1 and TIAM1, proteins absent in metastasis. Moreover, differential...

  12. The paradox of high availability and low recognition of soluble HLA-G by LILRB1 receptor in rheumatoid arthritis patients

    Veit, Tiago Degani; Chies, Jose Artur Bogo; Switala, Magdalena; Wagner, Bettina; Horn, Peter A.; Busatto, Mauricio; Brenol, Claiton Viegas; Brenol, João Carlos Tavares; Xavier, Ricardo Machado; Rebmann, Vera
    HLA-G is a regulatory molecule involved in immunologic tolerance. Growing evidence indicates that HLA-G plays a role in the regulation of inflammatory processes and autoimmune diseases. This study aimed at a systematic evaluation of soluble HLA-G (sHLA-G) in plasma of rheumatoid arthritis (RA) patients with long-lasting chronic inflammation. RA patients (n=68) and healthy controls (n=26) had their plasmatic sHLA-G measured by ELISA whereas the binding capability of sHLA-G to its cognate LILRB1 receptor was measured by a Luminex-based assay. All subjects were PCR-genotyped for HLA-G 14bp polymorphism (rs66554220). Significantly higher sHLA-G levels were observed in patients (p<0.001), however no significant...

  13. Metabotropic glutamate receptors as a new therapeutic target for malignant gliomas

    Pereira, Mery Stéfani Leivas; Klamt, Fabio; Thomé, Chairini Cássia; Worm, Paulo Valdeci; Oliveira, Diogo Losch de
    Metabotropic glutamate receptors (mGluR) are predominantly involved in maintenance of cellular homeostasis of central nervous system. However, evidences have suggested other roles of mGluR in human tumors. Aberrant mGluR signaling has been shown to participate in transformation and maintenance of various cancer types, including malignant brain tumors. This review intends to summarize recent findings regarding the involvement of mGluR-mediated intracellular signaling pathways in progression, aggressiveness, and recurrence of malignant gliomas, mainly glioblastomas (GBM), highlighting the potential therapeutic applications of mGluR ligands. In addition to the growing number of studies reporting mGluR gene or protein expression in glioma samples (resections, lineages,...

  14. Emergence of OXA-72-producing Acinetobacter baumannii belonging to high-risk clones (CC15 and CC79) in different brazilian states

    Pagano, Mariana; Rocha, Lisiane da Luz; Sampaio, Jorge Luiz Mello; Martins, Andreza Francisco; Barth, Afonso Luis
    Resumo não disponível

  15. Changes in astroglial markers in a maternal immune activation model of schizophrenia in wistar rats are dependent on sex

    Souza, Daniela Fraga de; Wartchow, Krista Minéia; Lunardi, Paula Santana; Brolese, Giovana; Tortorelli, Lucas Silva; Batassini, Cristiane; Biasibetti, Regina; Goncalves, Carlos Alberto Saraiva
    Data from epidemiological studies suggest that prenatal exposure to bacterial and viral infection is an important environmental risk factor for schizophrenia. The maternal immune activation (MIA) animal model is used to study how an insult directed at the maternal host can have adverse effects on the fetus, leading to behavioral and neurochemical changes later in life. We evaluated whether the administration of LPS to rat dams during late pregnancy affects astroglial markers (S100B and GFAP) of the offspring in later life. The frontal cortex and hippocampus were compared in male and female offspring on postnatal days (PND) 30 and 60....

  16. A proteomic insight into vitellogenesis during tick ovary maturation

    Xavier, Marina Amaral; Tirloni, Lucas; Pinto, Antonio Frederico Michel; Diedrich, Jolene K.; Yates III, John R.; Mulenga, Albert; Logullo, Carlos; Vaz Junior, Itabajara da Silva; Seixas, Adriana; Termignoni, Carlos
    Ticks are arthropod ectoparasites of importance for public and veterinary health. The understanding of tick oogenesis and embryogenesis could contribute to the development of novel control methods. However, to date, studies on the temporal dynamics of proteins during ovary development were not reported. In the present study we followed protein profile during ovary maturation. Proteomic analysis of ovary extracts was performed by liquid chromatography-tandem mass spectrometry (LC-MS/ MS) using shotgun strategy, in addition to dimethyl labelling-based protein quantification. A total of 3,756 proteins were identified, which were functionally annotated into 30 categories. Circa 80% of the annotated proteins belong to...

  17. Chronic exposure to ethanol causes steatosis and inflammation in zebrafish liver

    Schneider, Ana Cláudia Reis; Gregório, Cleandra; Cruz, Carolina Uribe; Guizzo, Ranieli; Malysz, Tais; Heuser, Maria Cristina Faccioni; Longo, Larisse; Silveira, Themis Reverbel da
    AIM To evaluate the effects of chronic exposure to ethanol in the liver and the expression of inflammatory genes in zebrafish. METHODS Zebrafish (n = 104), wild type, adult, male and female, were divided into two groups: Control and ethanol (0.05 v/v). The ethanol was directly added into water; tanks water were changed every two days and the ethanol replaced. The animals were fed twice a day with fish food until satiety. After two and four weeks of trial, livers were dissected, histological analysis (hematoxilineosin and Oil Red staining) and gene expression assessment of adiponectin, adiponectin receptor 2 (adipor2 ),...

  18. Effect of diacerein on metabolic control and inflammatory markers in patients with type 2 diabetes using antidiabetic agents : a randomized controlled trial

    Tres, Glaucia Sarturi; Fuchs, Sandra Cristina Pereira Costa; Piovesan, Fabiana; Santos, Patricia Koehler dos; Pereira, Fernanda dos Santos; Camey, Suzi Alves; Lisboa, Hugo Roberto Kurtz; Moreira, Leila Beltrami
    Introduction. Studies have shown that T2DM is an inflammatory disease. Thus, the present study was aimed at evaluating whether diacerein could improve the metabolic and inflammatory profile among patients with T2DM under long-term treatment with glucose-lowering agents. Methods. This is a double-blind, parallel, placebo-controlled trial with 72 participants randomly assigned to diacerein 50 mg or placebo for 12 weeks. The primary endpoint was the between-group difference in change in HbA1c. Secondary endpoints included the proportion of patients achieving metabolic control [HbA1c ≤ 7 0% (53 mmol/mol)] and change in inflammatory mediators. Results. Participants in the diacerein group had greater reductions...

  19. DNA damage in homocystinuria : 8-oxo-, 8-dihydro-2’-deoxyguanosine levels in cystathionine-β-synthase deficient patients and the in vitro protective effect of N-acetyl-L-cysteine

    Vanzin, Camila Simioni; Mescka, Caroline Paula; Marchetti, Desirèe Padilha; Donida, Bruna; Deon, Marion; Jacques, Carlos Eduardo Diaz; Hauschild, Tatiane Cristina; Faverzani, Jéssica Lamberty; Hauschild, Tatiane Cristina; Moura, Dinara Jaqueline; Saffi, Jenifer; Coelho, Daniella de Moura; Wajner, Moacir; Wyse, Angela Terezinha de Souza; Vargas, Carmen Regla
    Introduction: Homocysteine (Hcy) tissue accumulation occurs in a metabolic disease characterized biochemically by cystathionine β-synthase (CBS) deficiency and clinically by mental retardation, vascular problems, and skeletal abnormalities. Previous studies indicate the occurrence of DNA damage secondary to hyperhomocysteinemia and it was observed that DNA damage occurs in leukocytes from CBS-deficient patients. This study aimed to investigate whether an oxidative mechanism could be involved in DNA damage previously found and investigated the in vitro effect of N-acety-L-cysteine (NAC) on DNA damage caused by high Hcy levels. Methods: We evaluated a biomarker of oxidative DNA damage in the urine of CBS‑deficient patients,...

  20. Effects of transcranial direct current stimulation block remifentanil-induced hyperalgesia : a randomized, double-blind clinical trial

    Braulio, Gilberto; Passos, Sávio Cavalcante; Leite, Fabrício Moura; Schwertner, André; Stefani, Luciana Paula Cadore; Souza, Ana Cláudia de; Torres, Iraci Lucena da Silva; Fregni, Felipe; Caumo, Wolnei
    Background: Remifentanil-induced hyperalgesia (r-IH) involves an imbalance in the inhibitory and excitatory systems. As the transcranial Direct Current Stimulation (tDCS) modulates the thalamocortical synapses in a top-down manner, we hypothesized that the active (a)-t-DCS would be more effective than sham(s)-tDCS to prevent r-IH. We used an experimental paradigm to induce temporal summation of pain utilizing a repetitive cold test (rCOLDT) assessed by the Numerical Pain Score (NPS 0-10) and we evaluated the function of the descending pain modulatory system (DPMS) by the change on the NPS (0–10) during the conditioned pain modulation (CPM)-task (primary outcomes). We tested whether a-tDCS would...

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