Archive of life sciences journal literature at the U.S. National Institutes of Health (NIH), developed and managed by NIH's National Center for Biotechnology Information (NCBI) in the National Library of Medicine (NLM).
Open Access Articles
Zerhouni, Elias A.
Dong, Aiping; Zhou, Lan; Zhang, Xing; Stickel, Shawn; Roberts, Richard J.; Cheng, Xiaodong
We have determined the structure of a mutant (Q237W) of HhaI DNA methyltransferase, complexed with the methyl-donor product AdoHcy. The Q237W mutant proteins were crystallized in the monoclinic space group C2 with two molecules in the crystallographic asymmetric unit. Protein-protein interface calculations in the crystal lattices suggest that the dimer interface has the specific characteristics for homodimer protein-protein interactions, while the two active sites are spatially independent on the outer surface of the dimer. The solution behavior suggests the formation of HhaI dimers as well. The same HhaI dimer interface is also observed in the previously characterized binary (M.HhaI-AdoMet) and...
Varmus, H.; Klausner, R.; Zerhouni, E.; Acharya, T.; Daar, A. S.; Singer, P. A.
This week an international panel announces a list of 14 Grand Challenges in Global Health, and scientists throughout the world will be invited to submit grant proposals to pursue them with funds provided by the Bill & Melinda Gates Foundation. We describe the characteristics of these challenges and the process by which they were formulated and selected after receiving over 1000 responses to a "call for ideas" from the scientific community.
Lull, Cristina; Wichers, Harry J.; Savelkoul, Huub F. J.
We discuss current information on the ability of extracts and
isolated metabolites from mushrooms to modulate immune responses. This can
result in a more enhanced innate and acquired disease resistance. The major
immunomodulating effects of these active substances derived from mushrooms
include mitogenicity and activation of immune effector cells, such as
lymphocytes, macrophages, and natural killer cells, resulting in the
production of cytokines, including interleukins (ILs), tumor necrosis factor
alpha (TNF)-?, and interferon gamma
(INF)-?. In particular, the ability of selective mushroom
extracts to modulate the differentiation capacity of CD4+ T
cells to mature into TH1 and/or TH2 subsets will
be discussed. As a consequence these extracts
will have profound effects...
Beijer, L.; Rylander, R.
To assess if (1?3)-?-D-glucan, a microbial
cell wall agent normally present in pollen, has the ability to
produce pollenlike response, sensitive persons received a nasal
deposition of two doses of (1?3)-?-D-glucan.
The percentage of eosinophils and amount of eotaxin were measured
in nasal lavage 30 minutes and 24 hours after challenge. No
effect could be demonstrated. The absence of an inflammatory
response after (1?3)-?-D-glucan application
confirms earlier findings in inhalation studies.
Dong, Aiping; Zhou, Lan; Zhang, Xing; Stickel, Shawn; Roberts, Richard J.; Cheng, Xiaodong
We have determined the structure of a mutant (Q237W) of HhaI DNA methyltransferase, complexed with the methyl-donor product AdoHcy. The Q237W mutant proteins were crystallized in the monoclinic space group C2 with two molecules in the crystallographic asymmetric unit. Protein-protein interface calculations in the crystal lattices suggest that the dimer interface has the specific characteristics for homodimer protein-protein interactions, while the two active sites are spatially independent on the outer surface of the dimer. The solution behavior suggests the formation of HhaI dimers as well. The same HhaI dimer interface is also observed in the previously characterized binary (M.HhaI-AdoMet) and...
Varmus, H.; Klausner, R.; Zerhouni, E.; Acharya, T.; Daar, A. S.; Singer, P. A.
This week an international panel announces a list of 14 Grand Challenges in Global Health, and scientists throughout the world will be invited to submit grant proposals to pursue them with funds provided by the Bill & Melinda Gates Foundation. We describe the characteristics of these challenges and the process by which they were formulated and selected after receiving over 1000 responses to a "call for ideas" from the scientific community.