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UCL University College London Eprints (373.594 recursos)

UCL Eprints collects the work of UCL researchers and makes it freely available over the web, helping the worldwide scholarly community to discover UCL research. Institutional repositories like UCL Eprints complement the traditional academic publishing and scholarly communications processes. They raise the visibility of research and help to maximise its impact. UCL researchers are encouraged to deposit a copy of each journal article, conference paper, working paper, and any other research output, in the UCL Eprints at the earliest opportunity, ensuring that their research reaches as wide an audience as possible.

Mostrando recursos 1 - 20 de 373.101

  1. Screening for protein-protein interactions using Förster resonance energy transfer (FRET) and fluorescence lifetime imaging microscopy (FLIM)

    Margineanu, A; Chan, JJ; Kelly, DJ; Warren, SC; Flatters, D; Kumar, S; Katan, M; Dunsby, CW; French, PM
    We present a high content multiwell plate cell-based assay approach to quantify protein interactions directly in cells using Förster resonance energy transfer (FRET) read out by automated fluorescence lifetime imaging (FLIM). Automated FLIM is implemented using wide-field time-gated detection, typically requiring only 10 s per field of view (FOV). Averaging over biological, thermal and shot noise with 100's to 1000's of FOV enables unbiased quantitative analysis with high statistical power. Plotting average donor lifetime vs. acceptor/donor intensity ratio clearly identifies protein interactions and fitting to double exponential donor decay models provides estimates of interacting population fractions that, with calibrated donor...

  2. Eating disorder behaviours amongst adolescents: investigating classification, persistence and prospective associations with adverse outcomes using latent class models

    Micali, N; Horton, NJ; Crosby, RD; Swanson, SA; Sonneville, KR; Solmi, F; Calzo, JP; Eddy, KT; Field, AE
    Diagnostic criteria for eating disorders (ED) remain largely based on clinical presentations, but do not capture the full range of behaviours in the population. We aimed to derive an empirically based ED behaviour classification using behavioural and body mass index (BMI) indicators at three time-points in adolescence, and to validate classes investigating prospective associations with adverse outcomes. Adolescents from the Avon Longitudinal Study of Parents and Children (ALSPAC) provided data on ED at age 14 (n = 6615), 16 (n = 5888), and 18 years (n = 5100), and had weight and height measured. Psychological and behavioural outcomes were assessed at 15.5/16 and 17.5/18 years. We fit gender-...

  3. Theranostic Fibers for Simultaneous Imaging and Drug Delivery

    Jin, M; Yu, DG; Geraldes, CF; Williams, GR; Bligh, SW
    New methods for creating theranostic systems with simultaneous encapsulation of therapeutic, diagnostic, and targeting agents are much sought after. This work reports for the first time the use of coaxial electrospinning to prepare such systems in the form of core-shell fibers. Eudragit S100 was used to form the shell of the fibers, while the core comprised poly(ethylene oxide) loaded with the magnetic resonance contrast agent Gd(DTPA) (Gd(III) diethylenetriaminepentaacetate hydrate) and indomethacin as a model therapeutic agent. The fibers had linear cylindrical morphologies with clear core-shell structures, as demonstrated by electron microscopy. X-ray diffraction and differential scanning calorimetry proved that both...

  4. A genomic approach to therapeutic target validation identifies a glucose-lowering GLP1R variant protective for coronary heart disease

    Scott, RA; Freitag, DF; Li, L; Chu, AY; Surendran, P; Young, R; Grarup, N; Stancáková, A; Chen, Y; Varga, TV; Yaghootkar, H; Luan, J; Zhao, JH; Willems, SM; Wessel, J; Wang, S; Maruthur, N; Michailidou, K; Pirie, A; van der Lee, SJ; Gillson, C; Al Olama, AA; Amouyel, P; Arriola, L; Arveiler, D; Aviles-Olmos, I; Balkau, B; Barricarte, A; Barroso, I; Garcia, SB; Bis, JC; Blankenberg, S; Boehnke, M; Boeing, H; Boerwinkle, E; Borecki, IB; Bork-Jensen, J; Bowden, S; Caldas, C; Caslake, M; CVD50 consortium,; Cupples, LA; Cruchaga, C; Czajkowski, J; den Hoed, M; Dunn, JA; Earl, HM; Ehret, GB; Ferrannini, E; Ferrieres, J; Foltynie, T; Ford, I; Forouhi, NG; Gianfagna, F; Gonzalez, C; Grioni, S; Hiller, L; Jansson, JH; Jørgensen, ME; Jukema, JW; Kaaks, R; Kee, F; Kerrison, ND; Key, TJ; Kontto, J; Kote-Jarai, Z; Kraja, AT; Kuulasmaa, K; Kuusisto, J; Linneberg, A; Liu, C; Marenne, G; Mohlke, KL; Morris, AP; Muir, K; Müller-Nurasyid, M; Munroe, PB; Navarro, C; Nielsen, SF; Nilsson, PM; Nordestgaard, BG; Packard, CJ; Palli, D; Panico, S; Peloso, GM; Perola, M; Peters, A; Poole, CJ; Quirós, JR; Rolandsson, O; Sacerdote, C; Salomaa, V; Sánchez, MJ; Sattar, N; Sharp, SJ; Sims, R; Slimani, N; Smith, JA; Thompson, DJ; Trompet, S; Tumino, R; van der A, DL; van der Schouw, YT; Virtamo, J; Walker, M; Walter, K; GERAD_EC Consortium,; Neurology Working Group of the Cohorts for Heart,; Aging Research in Genomic Epidemiology (CHARGE),; Alzheimer’s Disease Genetics Consortium,; Pancreatic Cancer Cohort Consortium,; European Prospective Investigation into Cancer and Nutrition–Car,; EPIC-InterAct,; Abraham, JE; Amundadottir, LT; Aponte, JL; Butterworth, AS; Dupuis, J; Easton, DF; Eeles, RA; Erdmann, J; Franks, PW; Frayling, TM; Hansen, T; Howson, JM; Jørgensen, T; Kooner, J; Laakso, M; Langenberg, C; McCarthy, MI; Pankow, JS; Pedersen, O; Riboli, E; Rotter, JI; Saleheen, D; Samani, NJ; Schunkert, H; Vollenweider, P; O'Rahilly, S; CHARGE consortium,; CHD Exome+ Consortium,; CARDIOGRAM Exome Consortium,; Deloukas, P; Danesh, J; Goodarzi, MO; Kathiresan, S; Meigs, JB; Ehm, MG; Wareham, NJ; Waterworth, DM
    Regulatory authorities have indicated that new drugs to treat type 2 diabetes (T2D) should not be associated with an unacceptable increase in cardiovascular risk. Human genetics may be able to guide development of antidiabetic therapies by predicting cardiovascular and other health endpoints. We therefore investigated the association of variants in six genes that encode drug targets for obesity or T2D with a range of metabolic traits in up to 11,806 individuals by targeted exome sequencing and follow-up in 39,979 individuals by targeted genotyping, with additional in silico follow-up in consortia. We used these data to first compare associations of variants...

  5. Epigenetic profiling of ADHD symptoms trajectories: a prospective, methylome-wide study

    Walton, E; Pingault, JB; Cecil, CA; Gaunt, TR; Relton, CL; Mill, J; Barker, ED
    Attention-deficit/hyperactivity disorder (ADHD) is a prevalent developmental disorder, associated with a range of long-term impairments. Variation in DNA methylation, an epigenetic mechanism, is implicated in both neurobiological functioning and psychiatric health. However, the potential role of DNA methylation in ADHD symptoms is currently unclear. In this study, we examined data from the Avon Longitudinal Study of Parents and Children (ALSPAC)-specifically the subsample forming the Accessible Resource for Integrated Epigenomics Studies (ARIES)-that includes (1) peripheral measures of DNA methylation (Illumina 450k) at birth (n=817, 49% male) and age 7 (n=892, 50% male) and (2) trajectories of ADHD symptoms (7-15 years). We...

  6. Teasing apart explanations of a developmental delay in binding: experimental evidence from the comparison of SLI and Williams syndrome

    Perovic, A; Wexler, K
    This study investigates the knowledge of binding in 21 English-speaking children with SLI, aged 6;08-16;05, compared to 21 children with WS, language- and age-matched, and 21 language-matched control children, aged 4-7;10. Our results demonstrate no difficulties in the interpretation of reflexive or personal pronouns in SLI, revealing an intact knowledge of reflexive binding, as well as spared pragmatic functioning. Children with WS however show difficulties with their interpretation of pronouns, accepting the local binding of a pronoun, indicating impaired pragmatics. Not surprisingly, our language-matched typical controls, aged between 4-7;08 (M=5;03), showed a classic pattern of the Apparent Delay of Principle...

  7. Adding Narratives to Numbers in a Mixed Methods Study of Successful Ageing: The 6-Day Sample of the Scottish Mental Survey 1947

    Carpentieri, JD; Elliott, BJ; Brett, CB; Deary, IJ
    This article details the development and potential uses of a qualitative sub-study within a quantitative, longitudinal study of a Scottish cohort born in 1936 (the 6-Day Sample). Analysing narratives and other biographical interview material, we explore the potential of mixed methods research designs to improve the study of successful ageing, a widely used but contested concept. While acknowledging the critiques of successful ageing, we suggest that the concept can be improved by the adoption of mixed method research strategies that address key criticisms, in particular the lack of attention to older people's own voices. Including those voices in our study...

  8. Jeremy Bentham, 'The Psychology of Economic Man', and Behavioural Economics

    Quinn, M
    Section 1 briefly reviews first the received interpretation of Bentham, which sees him as having had little to do with the development of economics (excepting some passing mentions which recognize his deployment of the concept of utility or his reduction of human motivation to self-interest, and perhaps a note on his discussion of the concept of diminishing marginal utility); and second, the manner in which he applies his concept of rationality to political economy. In section 2, the central thesis of the paper is presented: It is argued that an examination of his insights into the psychology of individual choice...

  9. Contrasting effects of tropical cyclones on the annual survival of a pelagic seabird in the Indian Ocean

    Nicoll, MAC; Nevoux, M; Jones, CG; Ratcliffe, N; Ruhomaun, K; Tatayah, V; Norris, K
    Tropical cyclones are renowned for their destructive nature and are an important feature of marine and coastal tropical ecosystems. Over the last 40 years, their intensity, frequency and tracks have changed, partly in response to ocean warming, and future predictions indicate that these trends are likely to continue with potential consequences for human populations and coastal ecosystems. However, our understanding of how tropical cyclones currently affect marine biodiversity, and pelagic species in particular, is limited. For seabirds, the impacts of cyclones are known to be detrimental at breeding colonies, but impacts on the annual survival of pelagic adults and juveniles...

  10. Hot and bothered: using trait-based approaches to assess climate change vulnerability in reptiles

    Bohm, M; Cook, D; Ma, H; Davidson, AD; García, A; Tapley, B; Pearce-Kelly, P; Carr, J
    One-fifth of the world’s reptiles are currently estimated as threatened with extinction, primarily due to the immediate threats of habitat loss and overexploitation. Climate change presents an emerging slow-acting threat. However, few IUCN Red List assessments for reptiles explicitly consider the potential role of climate change as a threat. Thus, climate change vulnerability assessments can complement existing Red List assessments and highlight further, emerging priorities for conservation action. Here we present the first trait-based global climate change vulnerability assessment for reptiles to estimate the climate change vulnerability of a random representative sample of 1498 species of reptiles. We collected species-specific...

  11. Overcoming data deficiency in reptiles

    Bland, LM; Bohm, M
    We have no information on the risk of extinction of 21% of reptiles listed as Data Deficient on the Sampled Red List Index (SRLI), an indicator developed to track global change in species status. Data Deficient species are of high research priority, because they contribute to uncertainty in estimates of extinction risk and are neglected by conservation programmes. We review the causes of data deficiency in reptiles; the likely status of Data Deficient reptiles; and possible solutions for their re-assessment. We find that 52% of Data Deficient reptiles lack information on population status and trends, and that few species are...

  12. Investigation into the effects of high-Z nano materials in proton therapy

    Ahmad, R; Royle, G; Lourenço, A; Schwarz, M; Fracchiolla, F; Ricketts, K
    High-Z nano materials have been previously shown to increase the amount of dose deposition within the tumour due to an increase in secondary electrons. This study evaluates the effects of high-Z nano materials in combination with protons, and the impact of proton energy, nanoparticle material and concentration. These effects were studied in silico through Monte Carlo simulation and experimentally through a phantom study, with particular attention to macroscale changes to the Bragg peak in the presence of nanoparticles. Three nanoparticle materials were simulated (gold, silver and platinum) at three concentrations (0.01, 0.1 and 6.5 mg ml(-1)) at two clinical proton energies...

  13. Epidermal grafting versus split-thickness skin grafting for wound healing (EPIGRAAFT): study protocol for a randomised controlled trial

    Kanapathy, M; Hachach-Haram, N; Bystrzonowski, N; Harding, K; Mosahebi, A; Richards, T
    BACKGROUND: Split-thickness skin grafting (SSG) is an important modality for wound closure. However, the donor site becomes a second, often painful wound, which may take more time to heal than the graft site itself and holds the risk of infection and scarring. Epidermal grafting (EG) is an alternative method of autologous skin grafting that harvests only the epidermal layer of the skin by applying continuous negative pressure on the normal skin to raise blisters. This procedure has minimal donor site morbidity and is relatively pain-free, allowing autologous skin grafting in an outpatient setting. We plan to compare EG to SSG...

  14. A Simple fMRI Compatible Robotic Stimulator to Study the Neural Mechanisms of Touch and Pain

    Riillo, F; Bagnato, C; Allievi, AG; Takagi, A; Fabrizi, L; Saggio, G; Arichi, T; Burdet, E
    This paper presents a simple device for the investigation of the human somatosensory system with functional magnetic imaging (fMRI). PC-controlled pneumatic actuation is employed to produce innocuous or noxious mechanical stimulation of the skin. Stimulation patterns are synchronized with fMRI and other relevant physiological measurements like electroencephalographic activity and vital physiological parameters. The system allows adjustable regulation of stimulation parameters and provides consistent patterns of stimulation. A validation experiment demonstrates that the system safely and reliably identifies clusters of functional activity in brain regions involved in the processing of pain. This new device is inexpensive, portable, easy-to-assemble and customizable to...

  15. Nanotechnology: Changing of the guard

    Howorka, S
    Membrane proteins control access of ions and molecules to a cell's interior, shuttle cargo and information across the cell boundary, and determine the cell's shape. Engineering the function of these proteins is key to the development of vaccines, biofuels, biosensor elements, and research tools. However, the range of accessible architectures is limited, because protein engineering usually involves making relatively modest structural changes to existing protein structures; folding extensively altered polypeptides into defined structures is very challenging. Recent studies have shown that some membrane-protein functions can be mimicked with DNA nanostructures, which are easier to manipulate than their natural templates.

  16. Human subthalamic nucleus-medial frontal cortex theta phase coherence is involved in conflict and error related cortical monitoring

    Zavala, B; Tan, H; Ashkan, K; Foltynie, T; Limousin, P; Zrinzo, L; Zaghloul, K; Brown, P
    The medial prefrontal cortex (mPFC) is thought to control the shift from automatic to controlled action selection when conflict is present or when mistakes have been recently committed. Growing evidence suggests that this process involves frequency specific communication in the theta (4-8Hz) band between the mPFC and the subthalamic nucleus (STN), which is the main target of deep brain stimulation (DBS) for Parkinson's disease. Key in this hypothesis is the finding that DBS can lead to impulsivity by disrupting the correlation between higher mPFC oscillations and slower reaction times during conflict. In order to test whether theta band coherence between...

  17. Core and local skin temperature: 3–24 months old toddlers and comparison to adults

    Garcia-Souto, MDP; Dabnichki, P
    The thermoregulatory responses, thermal sensation and comfort level of children are very different to those of the adult population, and should be considered in the modelling of thermal comfort and the use of climate control systems in children-shared spaces. In addition, thermoregulation is believed to be related with Sudden Infant Death Syndrome, a major cause of death in children aged below 1 year. However experimental core and skin temperature data of the infant population is very limited, especially in toddlers. The present work investigates and reports the body core and local skin temperature characteristics of young children, as well as...

  18. CYP450-derived oxylipins mediate inflammatory resolution

    Gilroy, DW; Edin, ML; De Maeyer, RP; Bystrom, J; Newson, J; Lih, FB; Stables, M; Zeldin, DC; Bishop-Bailey, D
    Resolution of inflammation has emerged as an active process in immunobiology, with cells of the mononuclear phagocyte system being critical in mediating efferocytosis and wound debridement and bridging the gap between innate and adaptive immunity. Here we investigated the roles of cytochrome P450 (CYP)-derived epoxy-oxylipins in a well-characterized model of sterile resolving peritonitis in the mouse. Epoxy-oxylipins were produced in a biphasic manner during the peaks of acute (4 h) and resolution phases (24-48 h) of the response. The epoxygenase inhibitor SKF525A (epoxI) given at 24 h selectively inhibited arachidonic acid- and linoleic acid-derived CYP450-epoxy-oxlipins and resulted in a dramatic...

  19. Cosmesis and breast-related quality of life outcomes following intra-operative radiotherapy for early breast cancer - a sub-study of the TARGIT - a trial

    Corica, T; Nowak, AK; Saunders, CM; Bulsara, M; Taylor, M; Vaidya, JS; Baum, M; Joseph, DJ
    Purpose: The international randomized TARGIT-A trial compared risk-adapted single-dose intra-operative radiotherapy (TARGIT-IORT) to 3-7 weeks of daily conventional external beam radiotherapy (EBRT) in women with early breast cancer treatable with breast conserving surgery. TARGIT-A showed TARGIT-IORT to be non-inferior compared to EBRT in terms of reducing the risk of local cancer recurrence and found no difference in breast cancer survival however its effect on patient reported cosmesis and breast-related quality of life (QOL) have not yet been described. Methods and Materials: Longitudinal cosmesis and QOL data were collected from a sub-set of TARGIT-A participants who received TARGIT-IORT as a separate...

  20. Community interest obligations in international energy law: a European perspective

    Azaria, D

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