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UCL University College London Eprints (335,763 recursos)
UCL Eprints collects the work of UCL researchers and makes it freely available over the web, helping the worldwide scholarly community to discover UCL research. Institutional repositories like UCL Eprints complement the traditional academic publishing and scholarly communications processes. They raise the visibility of research and help to maximise its impact. UCL researchers are encouraged to deposit a copy of each journal article, conference paper, working paper, and any other research output, in the UCL Eprints at the earliest opportunity, ensuring that their research reaches as wide an audience as possible.

Mostrando recursos 161 - 180 de 335,186

161. Smith-Lemli-Opitz syndrome: A variable phenotype caused by 7 dehydro cholesterol reductase deficiency. - Ryan, AK; Bartlett, K; Clayton, P; Eaton, S; Mills, K; Collins, J; Donnai, D; Winter, R; Burn, J

162. Smith Lemli Opitz syndrome: a variable phenotype caused by 7 dehydro cholesterol reductase deficiency. - Ryan, AK; Barlett, K; Clayton, P; Eaton, S; Mills, K; Collins, J; Donnai, D; Winter, R; Burn, J

163. Effect of in vitro ischaemia on mitochondrial beta-oxidation in the heart. - Eaton, S; Bartlett, K

164. Inhibition of mitochondrial beta-oxidation in the heart by increased redox state of the ubiquinone pool. - Eaton, S; Bartlett, K

165. Quantitation of acyl-CoA and acylcarnitine esters accumulated during abnormal mitochondrial fatty acid oxidation. - Kler, RS; Jackson, S; Bartlett, K; Bindoff, LA; Eaton, S; Pourfarzam, M; Frerman, FE; Goodman, SI; Watmough, NJ; Turnbull, DM
We have used radio-high pressure liquid chromatography to study the acyl-CoA ester intermediates and the acylcarnitines formed during mitochondrial fatty acid oxidation. During oxidation of [U-14C]hexadecanoate by normal human fibroblast mitochondria, only the saturated acyl-CoA and acylcarnitine esters can be detected, supporting the concept that the acyl-CoA dehydrogenase step is rate-limiting in mitochondrial beta-oxidation. Incubations of fibroblast mitochondria from patients with defects of beta-oxidation show an entirely different profile of intermediates. Mitochondria from patients with defects in electron transfer flavoprotein and electron transfer flavoprotein:ubiquinone oxido-reductase are associated with slow flux through beta-oxidation and accumulation of long chain acyl-CoA and acylcarnitine...

166. Intermediates of peroxisomal beta-oxidation. A study of the fatty acyl-CoA esters which accumulate during peroxisomal beta-oxidation of [U-14C]hexadecanoate. - Bartlett, K; Hovik, R; Eaton, S; Watmough, NJ; Osmundsen, H
1. 14C-labelled fatty acyl-CoA esters resulting from beta-oxidation of [U-14C]hexadecanoate by peroxisomal fractions isolated from rats treated with clofibrate showed the presence of the full range of saturated intermediates down to acetyl-CoA. 2. The pattern of intermediates generated was fairly constant. At low concentrations of [U-14C]hexadecanoate (50 microM), decanoyl-CoA was present in lowest amounts. At higher concentrations of [U-14C]hexadecanoate (greater than 100 microM), all intermediates of chain length shorter than 12 carbon atoms (except acetyl-CoA) were present at similar low concentrations; the process of beta-oxidation now resembling chain-shortening of hexadecanoate by two cycles of beta-oxidation. 3. In the absence of...

167. Intramitochondrial control of the oxidation of hexadecanoate in skeletal muscle. A study of the acyl-CoA esters which accumulate during rat skeletal-muscle mitochondrial beta-oxidation of [U-14C]hexadecanoate and [U-14C]hexadecanoyl-carnitine. - Eaton, S; Bhuiyan, AK; Kler, RS; Turnbull, DM; Bartlett, K
1. We describe the acyl-CoA and acyl-carnitine esters which arise from the incubation of well-coupled State 3 rat skeletal-muscle mitochondrial fractions with [U-14C]hexadecanoate and [U-14C]hexadecanoyl-carnitine. 2. Acyl-CoA ester intermediates of chain length 16, 14, 12, 10 and 8 carbons were detected. 3. Although incubations were in steady state in respect of oxygen consumption, 14CO2 production and generation of acid-soluble radioactivity, quantitative analysis of acyl-CoA esters showed that steady state was not achieved in respect of all intermediates. 4. 3-Hydroxyacyl- and 2-enoyl-CoA and -carnitine esters were found under normoxic conditions. 5. Direct measurement of NAD+ and NADH shows that under identical...

168. Investigation of beta-oxidation in human liver biopsies. - Eaton, S; Record, CO; Bartlett, K

169. Production of 3-enoyl-CoA esters from palmitate by rat liver mitochondria. - Eaton, S; Turnbull, DM; Bartlett, K

170. Redox control of beta-oxidation in rat liver mitochondria. - Eaton, S; Turnbull, DM; Bartlett, K
Coupled rat liver mitochondria were incubated with [U-14C]hexadecanoate and carnitine which resulted in the formation of acyl-, 2-enoyl- and 3-hydroxyacyl-CoA and carnitine esters. The production of 2-enoyl-CoA and 3-hydroxyacyl-CoA esters was associated with a significant lowering of the NAD+/NADH ratio, in contrast to rat muscle mitochondria [Eaton, S., Bhuiyan, A. K. M. J., Kler, R. S., Turnbull, D. M. & Bartlett, K. (1993) Biochem. J. 289, 161-172], suggesting that control by the respiratory chain is important under normal conditions. When NAD+/NADH ratios were held low by succinate-induced reverse electron flow, 3-enoyl-CoA esters were also detected, probably formed by the action...

171. New developments in neonatal screening. - Bartlett, K; Eaton, SJ; Pourfarzam, M

172. Mitochondrial beta-oxidation in human alcoholic liver disease. - Eaton, S; Record, CO; Bartlett, K

173. An HPLC assay for sn-1,2-diacylglycerol. - Eaton, S; Shmueli, E; al-Mardini, H; Bartlett, K; Record, CO
A method for the analysis of 1,2-diacylglycerols in biological samples is presented. After tissue extraction and derivatisation with 3,5-dinitrobenzoyl chloride, samples are analysed by normal phase HPLC, using a 3.9 x 300 mm microPorasil column, and ultraviolet detection at 254 nm. The method gives quantitative recovery of 1,2-diacylglycerol, and is of sufficient sensitivity to allow quantitation of 1,2-diacylglycerol in human muscle needle biopsy specimens, from as little as 10 mg muscle. Human skeletal muscle from fasted control subjects was found to have a 1,2-diacylglycerol content of 455 +/- 78 nmol/g wet weight. The method is robust, giving intra- and inter-assay...

174. PKC{varepsilon}-Calcineurin Co-Signalling Downstream of TLR4 Downregulates Fibrosis and Induces Wound Healing Gene Expression in Cardiac Myofibroblasts. - Mesquita, RF; Paul, MA; Valmaseda, A; Francois, A; Jabr, R; Anjum, S; Marber, MS; Budhram-Mahadeo, V; Heads, RJ
The pathways which regulate resolution of inflammation and contribute to positive remodelling of the myocardium following injury are poorly understood. Here we show that protein kinase C epsilon (PKCε) co-operates with the phosphatase calcineurin (CN) to potentiate induction of cardioprotective gene expression whilst suppressing expression of fibrosis markers. This was achieved by detailed analysis of the regulation of cyclooxygenase-2 (COX-2) expression as a marker gene and by using gene expression profiling to identify genes regulated by co-expression of CN-Aα/PKCε in adult rat cardiac myofibroblasts (ARVFs) on a larger scale. Gene-chip analysis of CN-Aα/PKCε co-expressing ARVFs showed that COX-2 provides a...

175. Cell cycle arrest and morphological differentiation can occur in the absence of apoptosis in a neuronal cell line - Budhram-Mahadeo, V; Lillycrop, KA; Latchman, DS

176. Regulation of Hsp27 expression and cell survival by the POU transcription factor Brn3a - Farooqui-Kabir, SR; Marber, MS; Heads, RJ; Budhram-Mahadeo, V; Lewis, H; Latchman, DS

177. The Post-Dosaster Phase of Transitional Settlement: A Perspective From Typhoon Yolanda (Haiyan) in Eastern Philippines - Faure Walker, JP; Alexander, D; macabuag, J; Suppasri, A
In March 2014, four months after Typhoon Yolanda (Haiyan), a collaborative field survey between UCL-IRDR and Tohoku University-IRIDeS was conducted in order to compare the effects of domestic and imported aid and assistance on the quality and speed of recovery and the vulnerability during the transitional phase between the initial emergency and long-term recovery and reconstruction. The team, comprised of Dr. Joanna Faure Walker and Prof. David Alexander from UCL-IRDR, Mr Joshua Macabuag from UCL-EPICentre and Dr. Anawat Suppasri from IRIDeS at Tohoku University, conducted interviews and accompanying structural engineering surveys of the respondents' dwellings for 160 households in 12...

178. Self-similar solution for oblique impact of a water column with sharp front on a wall and its zero inner angle steady limit - Sun, SL; Wu, GX

179. The effect of respiratory chain impairment of beta-oxidation in rat heart mitochondria. - Eaton, S; Pourfarzam, M; Bartlett, K
Cardiac ischaemia leads to an inhibition of beta-oxidation flux and an accumulation of acyl-CoA and acyl-carnitine esters in the myocardium. However, there remains some uncertainty as to which esters accumulate during cardiac ischaemia and therefore the site of inhibition of beta-oxidation [Moore, Radloff, Hull and Sweely (1980) Am. J. Physiol. 239, H257-H265; Latipää (1989) J. Mol. Cell. Cardiol. 21, 765-771]. When beta-oxidation of hexadecanoyl-CoA in state III rat heart mitochondria was inhibited by titration of complex III activity, flux measured as 14CO2 release, acid-soluble radioactivity or as acetyl-carnitine was progressively decreased. Low concentrations of myxothiazol caused reduction of the ubiquinone...

180. Oosterhoff on Trusts - Oosterhoff, AH; Chambers, R; McInnes, M
Text, cases, and materials on the law of trusts in Canada

 

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