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Hokkaido University Collection of Scholarly and Academic Papers (135.521 recursos)

HUSCAP (Hokkaido University Collection of Scholarly and Academic Papers) contains peer-reviewed journal articles, proceedings, educational resources and any kind of scholarly works of Hokkaido University.

Japanese Journal of Veterinary Research

Mostrando recursos 1 - 20 de 242

  1. Erratum


  2. The SentinelTM EADR program can detect more microorganisms than bedding sentinel animals

    Niimi, Kimie; Maruyama, Shigeri; Sako, Norihisa; Miyata, Kazuyoshi; Yoshimoto, Takuro; Bilecki, Brian; Henderson, Kenneth. S.; Takahashi, Eiki
    Bedding sentinel programs have been the standard method for monitoring the health status of rodents housed in individually ventilated cages. However, it has been reported that some infectious microorganisms cannot be detected by bedding sentinels. Thus, more sensitive microbiological monitoring methods are needed. In the present study, we assessed the sensitivity of the SentinelTM EADR program, developed by Allentown Inc. and Charles River Laboratories Inc., which involves a combination of exhaust air dust and environmental PCR testing. We compared the sensitivity of SentinelTM EADR to that of bedding sentinels and fecal samples collected from mice housed in rooms. In conclusion,...

  3. Age-related change of the plasma fibrinogen concentration in dogs

    Segawa, Kazuhito; Takahashi, Chika; Takahashi, Makoto; Ito, Tetsuro; Neo, Sakurako; Hisasue, Masaharu; Tsuchiya, Ryo
    The aim of the present study was to investigate whether canine plasma fibrinogen concentration changes with age. We also investigated sex differences in the plasma fibrinogen concentration. As a result, the plasma fibrinogen concentration was found to moderately increase with age (Spearman’s r = 0.55, P < 0.001), whereas that of the male and female dogs did not differ to a statistically significant extent. This finding suggests the need to consider aging as a factor that affects the plasma fibrinogen concentration.

  4. Seroprevalence and Genotyping of Toxoplasma gondii from Free-range Chickens in Qassim, Saudi Arabia

    Nasr, Ibrahim Al; El-Ashram, Saeed; Huang, Shujian
    Toxoplasma gondii is an intracellular apicomplexan parasite that infects a wide range of warmblooded vertebrate hosts, including birds. The present study was undertaken to isolate and genotype T. gondii from free-range chickens (Gallus domesticus) in Qassim, Saudi Arabia. A total of 244 chickens were examined for T. gondii infection. Antibodies to T. gondii were detected in 29 (11.9%) chickens using the direct agglutination test (DAT). The brains of 29 chickens were bioassayed in mice. Four (13.8%) bioassayed mouse samples were positive for T. gondii DNA. Genotyping using the surface membrane antigen 3 (SAG3) locus indicated that all four isolates were...

  5. Effects of scatter correction processing on image quality of portable thoracic radiography in calves

    Shimbo, Genya; Tagawa, Michihito; Matsumoto, Kotaro; Tomihari, Mizuki; Miyahara, Kazuro
    Thoracic radiography provides diagnostic assistance in assessing the severity of lung lesions in bovine respiratory diseases. We investigated the effects of a novel scatter correction processing software on the image quality of portable thoracic radiography in calves: this scatter correction method was recently developed for mobile bedside chest radiography in humans. Thoracic radiographs of the caudodorsal region were obtained from 30 calves of various sizes. Scatter correction processing of a grid ratio of 3 : 1 or 8 : 1 was applied to each image. The delineation of caudal thoracic vertebral bodies, scapulae, proximal third of the ribs, pulmonary vessels,...

  6. Immune cellular responses to Sendai virus infection in D2.B6-Sen1Sen2Sen3 congenic mice, of which three quantitative trait loci responsible for the resistance to infection were introgressed from C57BL/6 mouse into DBA/2 mouse

    Abbas, Raghda M.F.; Tag-EL-Din-Hassan, Hassan T.; Boonyarattanasoonthorn, Tussapon; Aoshima, Keisuke; Morimatsu, Masami; Agui, Takashi
    It has been reported that C57BL/6 (B6) mice are resistant to the Sendai virus (SeV) infection, whereas DBA/2 (D2) mice are susceptible, the cause of susceptibility in D2 mice is hyper-inflammatory cytokine production, and three quantitative trait loci (QTLs), Sen1, Sen2, and Sen3 are identified to be responsible for this difference. We previously have verified that the introgression of B6-derived these three QTLs into D2-genetic background increases survival rate and suppresses cytokine production by generating D2.B6-Sen1Sen2Sen3 congenic mice. In this study, we investigated immune cellular responses of D2.B6-Sen1Sen2Sen3 mice after SeV infection. Body weight loss, viral load, immune cells in...

  7. A pilot study of transplantation of an autologous corneal epithelial cell sheet in a canine model of corneal injury

    Nam, Eunryel; Fujita, Naoki; Morita, Maresuke; Lin, Hsing-Yi; Endo, Kentaro; Nakagawa, Takayuki; Nishimura, Ryohei; Tsuzuki, Keiko
    Corneal transplantation is the most effective method of restoring corneal transparency. However, graft rejection due to immunoreaction may occur and prevent proper healing of the wounded corneal surface. To address this issue, we transplanted an autologous corneal epithelial sheet into the wounded corneal surface of a dog and evaluated the sheet’s efficiency and safety for clinical use. Four dogs with a wounded corneal surface— three experimental dogs receiving transplanted corneal epithelial cell sheets and a control dog who did not receive the transplant. We removed the entire layers of corneal epithelium and superficial stroma from each dog. Corneal epithelial sheets...

  8. Evaluation of body condition using body mass and chest girth in brown bears of Hokkaido, Japan (Ursus arctos yesoensis)

    Moriwaki, Jun; Omori, Ryosuke; Shimozuru, Michito; Tsuruga, Hifumi; Mano, Tsutomu; Tsubota, Toshio
    Chest girth and body mass of 3,576 brown bears (Ursus arctos yesoensis) harvested for conflict management in Hokkaido, Japan during 1991-2012 were used to establish methods to assess body condition and to compare the body condition of bears by sex, month, year, and reproductive status. The body mass was estimated based on the chest girth in cases with no measurements of the bear body mass. Using the measured and estimated body mass, a growth curve by age was demonstrated to ascertain the mean asymptotical body mass (245 kg for males, 114 kg for females) and ages at 95% asymptotic body...

  9. Study of stress in dairy cattle during student practical training on a farm

    Gin, Azusa; Sato, Takumi; Tohei, Atsushi; Miura, Ryotaro; Mizutani, Hisashi; Amao, Hiromi; Yamada, Yutaka; Kamiya, Shinji; Yosimura, Itaru; Mochizuki, Mariko
    We investigated changes in cortisol (COR) concentration, which is well known as an index of stress in the serum of dairy cattle. The COR concentrations in serum obtained from dairy cattle were collected during practical training of first-year students on a farm attached to the Nippon Veterinary and Life Science University. Mean COR concentration in serum determined after practical training was significantly higher (P < 0.001) than that in serum collected before training. Discriminant analysis was used to classify the relation between COR concentration of serum collected before and after practical training. In conclusion, the data was bipartite according to...

  10. Investigation of chromated copper arsenate-treated waste wood used for bedding material in the Hokkaido area

    Noda, Jun; Izumi, Kenichi; Tamura, Yutaka
    Chromated copper arsenate (CCA)-treated wood has prompted public concern regarding the contamination of livestock-rearing environment. We investigated the current status of CCA-treated waste wood used as bedding material for livestock in the Hokkaido area. Two-step surveys with structured question and purposive sampling were conducted to clarify the current status of woodbased bedding materials for contamination of CCA compounds in farms. The analyses of CCA compounds with an atomic absorption spectrometer indicated unusually high concentrations of chromium, copper, and arsenic in some bedding materials. These findings suggest that ca. 2 % (n = 84) of bedding materials for livestock are most...

  11. Discovery of genome of an immunodeficiency-associated virus-like virus from pig feces in Japan

    Oba, Mami; Katayama, Yukie; Tsuchiaka, Shinobu; Omatsu, Tsutomu; Murata, Yoshiteru; Ohya, Kenji; Makino, Shinji; Nagai, Makoto; Mizutani, Tetsuya
    Immunodeficiency-associated stool virus (IASV) is an unclassified virus, for which the only genome information is available from a patient infected by human immunodeficiency virus. In domestic animals, one report described detection of IASV-like virus in pig feces, whereas no nucleotide sequence information of this virus is currently available. Using deep sequencing method, we detected a DNA fragment homologous to IASV in several pig feces in Japan. The sequence of the PCR product in this sample had 70% homology to that of IASV. The infectious rate of the IASV-like virus was 72.9% among the 9 pig farms, from which the samples...

  12. Acute testicular atrophy in an active Thoroughbred stallion

    Toishi, Yuko; Tsunoda, Nobuo; Okamoto, Minoru; Watanabe, Minami; Katagiri, Seiji; Taya, Kazuyoshi
    A Thoroughbred stallion was used as an active stallion for ten years, however, the number and motility of sperm dramatically decreased and azoospermia occurred at 16 years of age. Circulating luteinizing hormone (LH) was high at 16 years of age compared to the levels before the occurrence of these symptoms. Testicular endocrine function was examined by human chorionic gonadotropin (hCG) administration. No increase in circulating testosterone was detected at 16 years of age, whereas two peaks of testosterone were observed with hCG treatment at 12 years of age. The number of Leydig cells in the testes increased, but the LH...

  13. Potency of an inactivated influenza vaccine prepared from A/duck/Mongolia/245/2015 (H10N3) against H10 influenza virus infection in a mouse model

    Suzuki, Mizuho; Okamatsu, Masatoshi; Fujimoto, Yuri; Hiono, Takahiro; Matsuno, Keita; Kida, Hiroshi; Sakoda, Yoshihiro
    The H10N8 influenza virus became a threat to public health when cases of fatal infections were identified in China in 2013 and 2014. Thus, genetic and antigenic characterization of H10 influenza viruses and development of an appropriate vaccine are essential to prepare for a future pandemic by H10 influenza viruses. However, current information regarding these properties of H10 influenza viruses circulating in birds is limited. In this study, genetic analysis of H10 influenza viruses revealed that the viruses recently circulating in wild birds in East Asia are genetically close to human H10N8 influenza viruses. Furthermore, the antigenicity of H10 influenza...

  14. Serological survey of severe fever with thrombocytopenia syndrome virus infection in Sika deer and rodents in Japan

    Lundu, Tapiwa; Yoshii, Kentaro; Kobayashi, Shintaro; Morikawa, Shigeru; Tsubota, Toshio; Misawa, Naoaki; Hayasaka, Daisuke; Kariwa, Hiroaki
    Severe fever with thrombocytopenia syndrome (SFTS) is a newly recognized zoonosis that occurs in China, Japan, and South Korea and is caused by the SFTS virus (SFTSV), which is in the genus Phlebovirus, family Phenuiviridae. Since its discovery in Japan in 2013, SFTS has been reported in the western parts of the country. To elucidate the distribution of SFTSV, we conducted a serological survey of deer and rodents. Serum was screened using enzyme-linked immunosorbent assay (ELISA) and suspected cases were further tested with an indirect immunofluorescence antibody (IFA) assay. Serum samples from 315 deer from Hokkaido (non-endemic area), 41 deer...

  15. Evaluation of grid performance for thoracic radiography in calves using a portable x-ray unit

    Shimbo, Genya; Tagawa, Michihito; Matsumoto, Kotaro; Tomihari, Mizuki; Miyahara, Kazuro
    Thoracic radiography provides diagnostic assistance in assessing the severity of lung lesions in bovine respiratory diseases. We investigated the effects of grid use on overall image quality for portable thoracic radiography in calves. Thoracic radiographs of the dorsocaudal region were made for 30 calves of various sizes, using three acquisition techniques (digital radiography with no grid, with a 3 : 1 grid, and with an 8 : 1 grid). Overall image quality was graded independently by five veterinarians using a 5-point scale based on the visibility of the thoracic vertebral bodies, thoracic spinous processes, scapulae, proximal third of the ribs,...

  16. Isolation and molecular characterization of extended-spectrum β-lactamase producing Escherichia coli from industrial food animals in Mekong Delta, Vietnam

    Hinenoya, Atsushi; Tran, Suong Thi Thu; Nguyen, Ngu Trong; Nguyen, Ha Cong; Nguyen, Doan Duy Le; Hoang, Phuong Hoai; Awasthi, Sharda Prasad; Hassan, Jayedul; Sumimura, Yoshinori; Yamamoto, Yoshimasa; Yamasaki, Shinji
    The aim of this study was to investigate if extended-spectrum β-lactamase producing Escherichia coli (ESBL-EC) is present in intestinal tracts of apparently healthy broiler chickens at large-scale chicken farm and pigs, and their environments in Vietnam. ESBL-EC was isolated from 86.7% cloacal swabs of chickens (13 out of 15), 55.0% rectal swabs of pigs (11 out of 20) and 100% from their surroundings (2 beddings in a chicken farm and 2 drainages in pig farms). All the isolates from chicken and pig farms were multidrug-resistant. Interestingly, 94.7% (36/38) isolates from chicken were resistant to ciprofloxacin and mcr-1 gene-positive (related to...

  17. Assessment of the growth inhibitory effect of gellan sulfate in rodent malaria in vivo

    Recuenco, Frances C.; Takano, Ryo; Sugi, Tatsuki; Takemae, Hitoshi; Murakoshi, Fumi; Ishiwa, Akiko; Inomata, Atsuko; Enomoto-Rogers, Yukiko; Fundador, Noreen Grace V.; Iwata, Tadahisa; Horimoto, Taisuke; Akashi, Hiroomi; Kato, Kentaro
    In our effort to discover novel antimalarials, we previously synthesized gellan sulfate, a novel anticoagulant derivative of gellan gum and showed that it inhibits the growth and invasion of erythrocytes by Plasmodium falciparum parasites in vitro (Recuenco et al. 2014. Sci Rep. 4:4723.). Gellan sulfate was also found to have low cytotoxicity and low anticoagulant activities in vitro. Here, we assessed gellan sulfate for its antimalarial effects in vivo. Despite its effectiveness in vitro, gellan sulfate inhibited the growth of both P. berghei ANKA and P. yoelii 17XL poorly in C57BL/6 and BALB/c mice, respectively. To our knowledge, this is the first...

  18. Case Report: Congenital methemoglobinemia in a cat with the reduced NADH-cytochrome b5 reductase 3 activity and missense mutations in CYB5R3

    Tani, Akiyoshi; Yamazaki, Jumpei; Nakamura, Kensuke; Takiguchi, Mitsuyoshi; Inaba, Mutsumi
    A one-year-old castrated male mixed-breed cat was referred for detailed examination of long-term pale mucous membrane without any clinical episodes that may cause cyanosis. While no causative abnormalities were detected in thoracic radiography and echocardiography, and arterial partial pressure of oxygen was within normal value, methemoglobin concentration of the cat was increased to 30% and cytochrome b5 reductase activity, which converts methemoglobin to hemoglobin, was reduced to 2.0 IU/gHb (13.4 ± 1.7 IU/gHb in control cats, n = 3), indicating congenital methemoglobinemia. Sequence analysis of CYB5R3, which codes cytochrome b5 reductase, showed two missense mutations found in the patient, one of which was...

  19. Comparative analysis of the reproductive tract microbial communities in female dogs with and without pyometra through the 16S rRNA gene pyrosequencing

    Young, Young Gang; Guevarra, Robin B.; Jun, Hyung Lee; Wattanaphansak, Suphot; Bit, Na Kang; Hyeun, Bum Kim; Kun, Ho Song
    Canine pyometra is one of the most common illnesses in middle-aged to aged bitches. We used the 16S rRNA gene analysis to evaluate whether there are differences in bacterial compositions between dogs with and without pyometra. Control vaginal swabs were obtained from clinically healthy bitches (n = 5) while uterus of bitches (n = 5) with pyometra were obtained through ovariohysterectomy. Results from this study showed that bacteria belonging to families Pasteurellaceae, Fusobacteriaceae and Porphyromonadaceae were abundant in the pyometra group. It is likely that these families comprise bacterial species which may be involved in the pathogenesis of canine pyometra. This...

  20. Fate and tissue depletion of nivalenol in ducks

    Amnart, Poapolathep; Saranya, Poapolathep; Piyaluk, Sinphithakkul; Kanjana, Imsilp; Supaporn, Isariyodom; Usuma, Jermnak; Phanwimol, Tanhan; Helen, Owen; Mario, Giorgi
    Nivalenol (NIV), a mycotoxin belonging to the trichothecenes type B group, has recently been identified as causing one of the more potent toxicities among mycotoxins of this group. The purpose of this study was to clarify the toxicokinetics, and residues of NIV in ducks. Then, NIV was administered intravenously (iv) or orally (po) to ducks at a dosage of 0.8 mg/kg body weight. The concentrations of NIV in plasma and various tissues were quantified using liquid chromatography tandem-mass spectrometry. The plasma concentrations of NIV were measurable up to 12 h after iv and po administrations, respectively. A non-compartmental model was used to...

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