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Hokkaido University Collection of Scholarly and Academic Papers (135.711 recursos)

HUSCAP (Hokkaido University Collection of Scholarly and Academic Papers) contains peer-reviewed journal articles, proceedings, educational resources and any kind of scholarly works of Hokkaido University.

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Mostrando recursos 1 - 20 de 461

  1. Chronological differential effects of pro-inflammatory cytokines on RANKL-induced osteoclast differentiation of canine bone marrow-derived macrophages

    Wijekoon, Suranji; Bwalya, Eugene C.; Fang, Jing; Kim, Sangho; Hosoya, Kenji; Okumura, Masahiro
    The aim of this study was to investigate osteoclastogenic properties of inflammatory cytokines at different time-points of osteoclastogenesis. Bone marrow-derived macrophages from five healthy dogs were stimulated with the macrophage colony-stimulating factor, receptor activator of nuclear factor-kappa B ligand and inflammatory cytokines such as interleukin (IL)-1 beta, tumor necrosis factor (TNF)-alpha and IL-17. Osteoclasts (OC) formation and function were enhanced with TNF-alpha regardless of temporal differences. But in contrast, IL-1 beta suppressed the osteoclastogenesis at early phase of the process while upregulating at the late phase. Furthermore, differentiation of OC precursors into OC was suppressed at high concentrations of IL-17....

  2. IL-36 alpha Regulates Tubulointerstitial Inflammation in the Mouse Kidney

    Ichii, Osamu; Kimura, Junpei; Okamura, Tadashi; Horino, Taro; Nakamura, Teppei; Sasaki, Hayato; Elewa, Yaser Hosny Ali; Kon, Yasuhiro
    IL-36 alpha, a member of the IL-1 family, is a crucial mediator of inflammatory responses. We previously found that IL-36 alpha was overexpressed in injured distal tubules (DTs); however, its pathological function remains unclear. Herein, unilateral ureter obstruction (UUO) or folic acid (FA) injection was performed in mouse kidneys to assess the role of IL-36 alpha in kidney injury. IL-36 alpha mRNA and protein expression significantly increased in the kidneys within 24 h after UUO. IL-36 alpha localized to dilated DTs. IL-36 alpha expression significantly correlated with the progression of tubulointerstitial cell infiltration and tubular epithelium cell death in UUO...

  3. Comparison of sperm subpopulation structures in first and second ejaculated semen from Japanese black bulls by a cluster analysis of sperm motility evaluated by a CASA system

    Kanno, Chihiro; Sakamoto, Kentaro Q.; Yanagawa, Yojiro; Takahashi, Yoshiyuki; Katagiri, Seiji; Nagano, Masashi
    In the present study, bull sperm in the first and second ejaculates were divided into subpopulations based on their motility characteristics using a cluster analysis of data from computer-assisted sperm motility analysis (CASA). Semen samples were collected from 4 Japanese black bulls. Data from 9,228 motile sperm were classified into 4 clusters; 1) very rapid and progressively motile sperm, 2) rapid and circularly motile sperm with widely moving heads, 3) moderately motile sperm with heads moving frequently in a short length, and 4) poorly motile sperm. The percentage of cluster 1 varied between bulls. The first ejaculates had a higher...

  4. Local CD34-positive capillaries decrease in mouse models of kidney disease associating with the severity of glomerular and tubulointerstitial lesions

    Masum, Md Abdul; Ichii, Osamu; Elewa, Yaser Hosny Ali; Nakamura, Teppei; Kon, Yasuhiro
    Background: The renal vasculature plays important roles in both homeostasis and pathology. In this study, we examined pathological changes in the renal microvascular in mouse models of kidney diseases. Methods: Glomerular lesions (GLs) in autoimmune disease-prone male BXSB/MpJ-Yaa (Yaa) mice and tubulointerstitial lesions (TILs) in male C57BL/6 mice subjected to unilateral ureteral obstruction (UUO) for 7 days were studied. Collected kidneys were examined using histopathological techniques. A nonparametric Mann-Whitney U test (P < 0.05) was performed to compare healthy controls and the experimental mice. The Kruskal-Wallis test was used to compare three or more groups, and multiple comparisons were performed...

  5. Contamination status and accumulation characteristics of heavy metals and arsenic in five seabird species from the central Bering Sea

    Ishii, Chihiro; Ikenaka, Yoshinori; Nakayama, Shouta M. M.; Mizukawa, Hazuki; Yohannes, Yared Beyene; Watanuki, Yutaka; Fukuwaka, Masaaki; Ishizuka, Mayumi
    Seabirds are marine top predators and accumulate high levels of metals and metalloids in their tissues. Contamination by metals in the highly productive offshore region has become a matter of public concern. It is home to 80% of the seabird population in the U.S.A., 95% of northern fur seals (Callorhinus ursinus), and major populations of Steller sea lions (Eumetopias jubatus), walruses (Odobenus rosmarus) and whales. Here, the concentrations of eight heavy metals (Hg, Cd, Cr, Co, Ni, Cu, Zn and Pb) and a metalloid (As) in the liver and kidneys of the northern fulmar (Fulmarus glacialis), thick-billed murre (Uria lomvia),...

  6. Listeria monocytogenes serotype 4b strains replicate in monocytes/macrophages more than the other serotypes

    Hasebe, Rie; Nakao, Ryo; Ohnuma, Aiko; Yamasaki, Takeshi; Sawa, Hirofumi; Takai, Shinji; Horiuchi, Motohiro
    We analyzed the pathogenicity of various serotypes of Listeria monocytogenes using a Balb/c mouse intravenous injection model. The survival rates of mice inoculated with strains NS1/2b (serotype 1/2b), NS3b (serotype 3b) and NS 4b (serotype 4b) were 60, 63.6 and 63.6%, respectively. Although the survival rates were similar, the bacterial growth in the liver of NS3b-infected mice was 144.5-fold higher than that in the liver of NS4b-infected mice. Histopathological analyses suggest that the NS4b strain replicated more in monocytes/macrophages, whereas the NS3b strain replicated more in hepatocytes. These results raise a possibility that the serotype 4b strains replicated more in...

  7. Cell-cycle arrest in mature adipocytes impairs BAT development but not WAT browning, and reduces adaptive thermogenesis in mice

    Okamatsu-Ogura, Yuko; Fukano, Keigo; Tsubota, Ayumi; Nio-Kobayashi, Junko; Nakamura, Kyoko; Morimatsu, Masami; Sakaue, Hiroshi; Saito, Masayuki; Kimura, Kazuhiro
    We previously reported brown adipocytes can proliferate even after differentiation. To test the involvement of mature adipocyte proliferation in cell number control in fat tissue, we generated transgenic (Tg) mice over-expressing cell-cycle inhibitory protein p27 specifically in adipocytes, using the aP2 promoter. While there was no apparent difference in white adipose tissue (WAT) between wild-type (WT) and Tg mice, the amount of brown adipose tissue (BAT) was much smaller in Tg mice. Although BAT showed a normal cellular morphology, Tg mice had lower content of uncoupling protein 1 (UCP1) as a whole, and attenuated cold exposure-or beta 3-adrenergic receptor (AR)...

  8. Lead exposure in raptors from Japan and source identification using Pb stable isotope ratios

    Ishii, Chihiro; Nakayama, Shouta M. M.; Ikenaka, Yoshinori; Nakata, Hokuto; Saito, Keisuke; Watanabe, Yukiko; Mizukawa, Hazuki; Tanabe, Shinsuke; Nomiyama, Kei; Hayashi, Terutake; Ishizuka, Mayumi
    Lead (Pb) poisoning is widespread among raptors and water birds. In Japan, fragments of Pb ammunition are still found in endangered eagles although more than 10 years have passed since legislation regarding use of Pb ammunition was introduced. This study was performed to investigate Pb exposure in raptors from various locations in Japan. We measured hepatic and renal Pb concentrations and hepatic Pb isotope ratios of Steller's sea eagles (Haliaeetus pelagicus), white-tailed sea eagles (Haliaeetus albicilla), golden eagles (Aquila chrysaetos), and 13 other species (total 177 individuals) that were found dead, as well as blood samples from three eagles found...

  9. Retinoic acid modulates lipid accumulation glucose concentration dependently through inverse regulation of SREBP-1 expression in 3T3L1 adipocytes

    Abd Eldaim, Mabrouk Attia; Matsuoka, Shinya; Okamatsu-Ogura, Yuko; Kamikawa, Akihiro; Ahmed, Mohamed Mohamed; Terao, Akira; Nakajima, Kei-ichi; Kimura, Kazuhiro
    It is well known that retinoic acid (RA) suppresses adipogenesis, although there are some contradicting reports. In this study, we examined the effect of extracellular glucose on RA-induced suppression of adipogenesis in 3T3L1 cell culture. When the cells were cultured in normal glucose medium (NG), the addition of RA suppressed lipid accumulation. However, when cultured in high glucose medium (HG), addition of RA to the cells enhanced lipid accumulation. These changes were accompanied by parallel alterations in fatty acid synthase (FAS) and sterol regulatory element-binding protein (SREBP)-1 gene expression. Transfection of SREBP-1 siRNA suppressed RA-induced enhancement of lipid accumulation and...

  10. Serial measurement of pancreatic lipase immunoreactivity concentration in dogs with immune-mediated disease treated with prednisolone

    Ohta, H.; Morita, T.; Yokoyama, N.; Osuga, T.; Sasaki, N.; Morishita, K.; Nakamura, K.; Takiguchi, M.
    Objectives: In this pilot study, serum canine pancreatic lipase immunoreactivity was measured repeatedly in dogs with various immune-mediated diseases that were treated with immunosuppressive doses of prednisolone. Methods: Ten client-owned dogs with newly diagnosed immune-mediated disease that had normal canine pancreatic lipase immunoreactivity concentrations (200 mu g/l) were treated with 2 to 2.2 mg/kg prednisolone orally once daily as the initial treatment. Serum samples were obtained from each of the dogs prior to treatment and at 1- to 4-week intervals during immunosuppressive treatment. The highest canine pancreatic lipase immunoreactivity concentration detected during immunosuppressive treatment was defined as the peak canine...

  11. Erratum

  12. The SentinelTM EADR program can detect more microorganisms than bedding sentinel animals

    Niimi, Kimie; Maruyama, Shigeri; Sako, Norihisa; Miyata, Kazuyoshi; Yoshimoto, Takuro; Bilecki, Brian; Henderson, Kenneth. S.; Takahashi, Eiki
    Bedding sentinel programs have been the standard method for monitoring the health status of rodents housed in individually ventilated cages. However, it has been reported that some infectious microorganisms cannot be detected by bedding sentinels. Thus, more sensitive microbiological monitoring methods are needed. In the present study, we assessed the sensitivity of the SentinelTM EADR program, developed by Allentown Inc. and Charles River Laboratories Inc., which involves a combination of exhaust air dust and environmental PCR testing. We compared the sensitivity of SentinelTM EADR to that of bedding sentinels and fecal samples collected from mice housed in rooms. In conclusion,...

  13. Age-related change of the plasma fibrinogen concentration in dogs

    Segawa, Kazuhito; Takahashi, Chika; Takahashi, Makoto; Ito, Tetsuro; Neo, Sakurako; Hisasue, Masaharu; Tsuchiya, Ryo
    The aim of the present study was to investigate whether canine plasma fibrinogen concentration changes with age. We also investigated sex differences in the plasma fibrinogen concentration. As a result, the plasma fibrinogen concentration was found to moderately increase with age (Spearman’s r = 0.55, P < 0.001), whereas that of the male and female dogs did not differ to a statistically significant extent. This finding suggests the need to consider aging as a factor that affects the plasma fibrinogen concentration.

  14. Seroprevalence and Genotyping of Toxoplasma gondii from Free-range Chickens in Qassim, Saudi Arabia

    Nasr, Ibrahim Al; El-Ashram, Saeed; Huang, Shujian
    Toxoplasma gondii is an intracellular apicomplexan parasite that infects a wide range of warmblooded vertebrate hosts, including birds. The present study was undertaken to isolate and genotype T. gondii from free-range chickens (Gallus domesticus) in Qassim, Saudi Arabia. A total of 244 chickens were examined for T. gondii infection. Antibodies to T. gondii were detected in 29 (11.9%) chickens using the direct agglutination test (DAT). The brains of 29 chickens were bioassayed in mice. Four (13.8%) bioassayed mouse samples were positive for T. gondii DNA. Genotyping using the surface membrane antigen 3 (SAG3) locus indicated that all four isolates were...

  15. Effects of scatter correction processing on image quality of portable thoracic radiography in calves

    Shimbo, Genya; Tagawa, Michihito; Matsumoto, Kotaro; Tomihari, Mizuki; Miyahara, Kazuro
    Thoracic radiography provides diagnostic assistance in assessing the severity of lung lesions in bovine respiratory diseases. We investigated the effects of a novel scatter correction processing software on the image quality of portable thoracic radiography in calves: this scatter correction method was recently developed for mobile bedside chest radiography in humans. Thoracic radiographs of the caudodorsal region were obtained from 30 calves of various sizes. Scatter correction processing of a grid ratio of 3 : 1 or 8 : 1 was applied to each image. The delineation of caudal thoracic vertebral bodies, scapulae, proximal third of the ribs, pulmonary vessels,...

  16. Immune cellular responses to Sendai virus infection in D2.B6-Sen1Sen2Sen3 congenic mice, of which three quantitative trait loci responsible for the resistance to infection were introgressed from C57BL/6 mouse into DBA/2 mouse

    Abbas, Raghda M.F.; Tag-EL-Din-Hassan, Hassan T.; Boonyarattanasoonthorn, Tussapon; Aoshima, Keisuke; Morimatsu, Masami; Agui, Takashi
    It has been reported that C57BL/6 (B6) mice are resistant to the Sendai virus (SeV) infection, whereas DBA/2 (D2) mice are susceptible, the cause of susceptibility in D2 mice is hyper-inflammatory cytokine production, and three quantitative trait loci (QTLs), Sen1, Sen2, and Sen3 are identified to be responsible for this difference. We previously have verified that the introgression of B6-derived these three QTLs into D2-genetic background increases survival rate and suppresses cytokine production by generating D2.B6-Sen1Sen2Sen3 congenic mice. In this study, we investigated immune cellular responses of D2.B6-Sen1Sen2Sen3 mice after SeV infection. Body weight loss, viral load, immune cells in...

  17. A pilot study of transplantation of an autologous corneal epithelial cell sheet in a canine model of corneal injury

    Nam, Eunryel; Fujita, Naoki; Morita, Maresuke; Lin, Hsing-Yi; Endo, Kentaro; Nakagawa, Takayuki; Nishimura, Ryohei; Tsuzuki, Keiko
    Corneal transplantation is the most effective method of restoring corneal transparency. However, graft rejection due to immunoreaction may occur and prevent proper healing of the wounded corneal surface. To address this issue, we transplanted an autologous corneal epithelial sheet into the wounded corneal surface of a dog and evaluated the sheet’s efficiency and safety for clinical use. Four dogs with a wounded corneal surface— three experimental dogs receiving transplanted corneal epithelial cell sheets and a control dog who did not receive the transplant. We removed the entire layers of corneal epithelium and superficial stroma from each dog. Corneal epithelial sheets...

  18. Evaluation of body condition using body mass and chest girth in brown bears of Hokkaido, Japan (Ursus arctos yesoensis)

    Moriwaki, Jun; Omori, Ryosuke; Shimozuru, Michito; Tsuruga, Hifumi; Mano, Tsutomu; Tsubota, Toshio
    Chest girth and body mass of 3,576 brown bears (Ursus arctos yesoensis) harvested for conflict management in Hokkaido, Japan during 1991-2012 were used to establish methods to assess body condition and to compare the body condition of bears by sex, month, year, and reproductive status. The body mass was estimated based on the chest girth in cases with no measurements of the bear body mass. Using the measured and estimated body mass, a growth curve by age was demonstrated to ascertain the mean asymptotical body mass (245 kg for males, 114 kg for females) and ages at 95% asymptotic body...

  19. Study of stress in dairy cattle during student practical training on a farm

    Gin, Azusa; Sato, Takumi; Tohei, Atsushi; Miura, Ryotaro; Mizutani, Hisashi; Amao, Hiromi; Yamada, Yutaka; Kamiya, Shinji; Yosimura, Itaru; Mochizuki, Mariko
    We investigated changes in cortisol (COR) concentration, which is well known as an index of stress in the serum of dairy cattle. The COR concentrations in serum obtained from dairy cattle were collected during practical training of first-year students on a farm attached to the Nippon Veterinary and Life Science University. Mean COR concentration in serum determined after practical training was significantly higher (P < 0.001) than that in serum collected before training. Discriminant analysis was used to classify the relation between COR concentration of serum collected before and after practical training. In conclusion, the data was bipartite according to...

  20. Preclinical study on hypoxic radiosensitizing effects of glycididazole in comparison with those of doranidazole in vitro and in vivo

    Yasui, Hironobu; Kubota, Nobuo; Nishizumi, Junko; Sakai, Yuri; Yamamori, Tohru; Inanami, Osamu
    To overcome the radioresistance of hypoxic cells in solid tumor, numerous types of radiosensitizers specifically against them have been developed. Glycididazole has a chemical structure in which two metronidazole forms are combined, and is widely used as a hypoxic radiosensitizer in China. However, a detailed investigation of its radiosensitizing properties has not been performed. The present study reported a comparative assessment of glycididazole and doranidazole, another hypoxic radiosensitizer. All experiments were performed using the murine squamous cell carcinoma cell line SCCVII. Prior to X‑irradiation, the cells were treated with the test drugs at concentrations of 10 mM and 200 mg/kg...

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