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Hokkaido University Collection of Scholarly and Academic Papers (135.521 recursos)

HUSCAP (Hokkaido University Collection of Scholarly and Academic Papers) contains peer-reviewed journal articles, proceedings, educational resources and any kind of scholarly works of Hokkaido University.

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Mostrando recursos 1 - 20 de 861

  1. Comparison of human papillomavirus genotyping and cytology triage, COMPACT Study : Design, methods and baseline results in 14 642 women

    Aoyama-Kikawa, Satomi; Fujita, Hiromasa; Hanley, Sharon J.B; Kasamo, Mitsunori; Kikuchi, Kokichi; Torigoe, Toshihiko; Matsuno, Yoshihiro; Tamakoshi, Akiko; Sasaki, Takayuki; Matsuura, Motoki; Kato, Yasuhito; Dong, Peixin; Watari, Hidemichi; Saito, Tsuyoshi; Sengoku, Kazuo; Sakuragi, Noriaki
    Although cytology‐based screening programs have significantly reduced mortality and morbidity from cervical cancer, the global consensus is that primary human papillomavirus (HPV) testing for cervical screening increases detection of high‐grade cervical intraepithelial neoplasia (CIN) and invasive cancer. However, the optimal triage strategy for HPV‐positive women to avoid over‐referral to colposcopy may be setting specific. As Japan requires data that have been generated domestically to modify screening guidelines, we conducted a 3‐year prospective study, COMparison of HPV genotyping And Cytology Triage (COMPACT), to evaluate the potential role of HPV16/18 partial genotyping and cytology for primary HPV screening. In total, 14 642...

  2. miR-34a, miR-424 and miR-513 inhibit MMSET expression to repress endometrial cancer cell invasion and sphere formation

    Dong, Peixin; Xiong, Ying; Yue, Junming; Hanley, Sharon J.B; Watari, Hidemichi
    Although the oncogene MMSET (also known as NSD2 or WHSC1) has an essential role in malignancies, its impact on human endometrial cancer (EC) metastasis and the molecular mechanism of MMSET regulation are largely unknown. We report that MMSET was markedly upregulated in EC cell lines and EC tissues, and was significantly associated with poor survival in EC. MMSET overexpression greatly promoted EC cell invasion and sphere formation, whereas inhibition of MMSET reduced EC cell invasion and sphere formation. Importantly, Twist1 was required for MMSET-induced EC cell invasion and sphere formation. Moreover, we demonstrate that miR-34a, miR-424 and miR-513 directly modulate...

  3. Post-partum podocyturia following pre-eclamptic pregnancy

    Furuta, Itsuko; Zhai, Tianyue; Umazume, Takeshi; Ishikawa, Satoshi; Hosokawa, Ami; Kojima, Takashi; Chiba, Kentaro; Yamada, Takahiro; Morikawa, Mamoru; Minakami, Hisanori
    Aim: Urine podocin mRNA expression and urine podocin : nephrin mRNA expression ratio (PNR) increase with increasing proteinuria during pregnancy complicated with pre-eclampsia (PE). This suggests that urine podocytes with reduced nephrin mRNA expression are abundant in pathological podocyturia. The aim of this study was therefore to determine post-partum changes in podocyturia and PNR in relation to proteinuria after pre-eclampsia (PE). Methods: A total of 137 peripartum urine specimens, consisting of 72 and 65 from 24 and 30 women with PE and normotensive control pregnancies (NCP), respectively, were studied. Determination of urine protein and creatinine concentration and quantitative analysis of...

  4. Effects of childbirth on podocyturia in women with normotensive, uncomplicated pregnancies

    Furuta, Itsuko; Zhai, Tianyue; Umazume, Takeshi; Ishikawa, Satoshi; Nakagawa, Kinuko; Kojima, Takashi; Yamada, Takahiro; Morikawa, Mamoru; Minakami, Hisanori
    Changes in hemodynamics and blood pressure occur shortly before and after childbirth regardless of the mode of delivery. This study aimed to test the hypothesis that parturition induces a temporal increase in podocyturia monitored by podocyte-specific protein podocin mRNA expression levels (Pod-mRNA). A total of 105 urine specimens, consisting of 43 and 62 from 18 and 20 otherwise healthy women with vaginal delivery (VD) and elective cesarean delivery (ECS), respectively, were studied. Determination of urine protein and creatinine (Cr) concentrations and quantitative analyses of Pod-mRNA, nephrin mRNA (Nep-mRNA), synaptopodin mRNA (Syn-mRNA), and aquaporin 2 mRNA expression were performed using RT-PCR...

  5. Risk factors of human herpesvirus 6 encephalitis/myelitis after allogeneic hematopoietic stem cell transplantation

    Miyashita, Naohiro; Endo, Tomoyuki; Onozawa, Masahiro; Hashimoto, Daigo; Kondo, Takeshi; Fujimoto, Katsuya; Kahata, Kaoru; Sugita, Junichi; Goto, Hideki; Matsukawa, Toshihiro; Hashino, Satoshi; Teshima, Takanori
    Background: Human herpesvirus 6 (HHV-6) encephalitis/myelitis is now a well-known complication after allogeneic stem cell transplantation (allo-HSCT), particularly after cord blood transplantation (CBT). In this study, we evaluated the risk factors of HHV-6 encephalitis/myelitis. Methods: We evaluated 253 patients who received allo-HSCT from 2007 to 2015 at our institute. HHV-6 encephalitis/myelitis was defined as HHV-6 DNA detection in the cerebrospinal fluid or peripheral blood by polymerase chain reaction in the presence of typical manifestations without other concurrent condition that led to the manifestations. Results: HHV-6 encephalitis/myelitis occurred in 11 patients (4.5%) (9 encephalitis, 3.7%; 2 myelitis, 0.8%). Multivariate analysis showed...

  6. Refining prognosis in patients with hepatocellular carcinoma through incorporation of metabolic imaging biomarkers

    Takeuchi, Satoshi; Rohren, Eric M.; Abdel-Wahab, Reham; Xiao, Lianchun; Morris, Jeffrey S.; Macapinlac, Homer A.; Hassan, Manal M.; Kaseb, Ahmed O.
    Purpose: 18F-fluorodeoxyglucose positron emission tomopraphy/computed tomography (FDGPET/CT) has been proven to be useful for imaging many types of cancer; however, its role is not well defined in hepatocellular carcinoma (HCC). We assessed the prognostic value of metabolic imaging biomarkers as established by baseline pretreatment FDG PET/CT in patients with HCC. Methods: We retrospectively analyzed the records of patients with HCC who underwent FDG PET/CT before initial treatment from May 2013 through May 2014. Four PET/CT parameters were measured: maximum standardized uptake value (SUVmax), total lesion glycolysis (TLG), metabolic tumor volume (MTV), and tumor-to-normal-liver SUV ratio (TNR). Optimal cut-off values for...

  7. 18F-FDG PET findings of pericardial lymphangiohemangioma

    Aikawa, Tadao; Naya, Masanao; Oyama-Manabe, Noriko; Maekawa, Satoshi; Nambu, Hideo; Mitsuyama, Hirofumi; Hirata, Kenji; Kanno-Okada, Hiromi; Tsutsui, Hiroyuki

  8. Prospective study to evaluate the safety of the world-first spot-scanning dedicated, small 360-degree gantry, synchrotron-based proton beam therapy system

    Nishioka, Kentaro; Prayongrat, Anussara; Ono, Kota; Onodera, Shunsuke; Hashimoto, Takayuki; Katoh, Norio; Inoue, Tetsuya; Kinoshita, Rumiko; Yasuda, Koichi; Mori, Takashi; Onimaru, Rikiya; Shirato, Hiroki; Shimizu, Shinichi
    This is a report of a single-institution prospective study evaluating the safety of a spot-scanning dedicated, small 360-degree gantry, synchrotron-based proton beam therapy (PBT) system. Data collection was performed for 56 patients with 59 treatment sites who received proton beam therapy at Hokkaido University Hospital between March 2014 and July 2015. Forty-one patients were male and 15 were female. The median age was 66 years. The primary lesion sites were prostate (n = 17), bone/soft tissue (n = 10), liver (n = 7), lung (n = 6), central nervous system (n = 5), colon (n = 2), pancreas (n =...

  9. The effect of duration of illness and antipsychotics on subcortical volumes in schizophrenia : Analysis of 778 subjects

    Hashimoto, Naoki; Ito, Yoichi M.; Okada, Naohiro; Yamamori, Hidenaga; Yasuda, Yuka; Fujimoto, Michiko; Kudo, Noriko; Takemura, Ariyoshi; Son, Shuraku; Narita, Hisashi; Yamamoto, Maeri; Tha, Khin Khin; Katsuki, Asuka; Ohi, Kazutaka; Yamashita, Fumio; Koike, Shinsuke; Takahashi, Tsutomu; Nemoto, Kiyotaka; Fukunaga, Masaki; Onitsuka, Toshiaki; Watanabe, Yoshiyuki; Yamasue, Hidenori; Suzuki, Michio; Kasai, Kiyoto; Kusumi, Ichiro; Hashimoto, Ryota
    Background: The effect of duration of illness and antipsychotic medication on the volumes of subcortical structures in schizophrenia is inconsistent among previous reports. We implemented a large sample analysis utilizing clinical data from 11 institutions in a previous meta-analysis. Methods: Imaging and clinical data of 778 schizophrenia subjects were taken from a prospective meta-analysis conducted by the COCORO consortium in Japan. The effect of duration of illness and daily dose and type of antipsychotics were assessed using the linear mixed effect model where the volumes of subcortical structures computed by FreeSurfer were used as a dependent variable and age, sex,...

  10. Increased podocyturia in pregnant women compared to non-pregnant women

    Furuta, Itsuko; Zhai, Tianyue; Umazume, Takeshi; Ishikawa, Satoshi; Nakagawa, Kinuko; Akaishi, Rina; Yamada, Takahiro; Morikawa, Mamoru; Minakami, Hisanori
    Aim: Hyperfiltration is a cause of podocyturia and occurs physiologically in the kidney of pregnant women. Podocyturia is increased in preeclamptic pregnancies, but it is unclear whether there is also any increase in uncomplicated pregnancies. This study was performed to examine whether podocyturia and urine aquaporin 2 mRNA expression are increased in healthy pregnant women (PW) compared to healthy non-pregnant women (NPW). Methods: Eleven urines obtained from 11 NPW and longitudinal 76 urines from 40 PW with uncomplicated pregnancies (median number [range] of urine samples/person, 2 [1 - 3]) were studied. Determination of protein and creatinine concentrations and number of...

  11. Loss of luteotropic prostaglandin E plays an important role in the regulation of luteolysis in women

    Nio-Kobayashi, Junko; Kudo, Masataka; Sakuragi, Noriaki; Iwanaga, Toshihiko; Duncan, W. Colin
    STUDY QUESTION: Do intraluteal prostaglandins (PG) contribute to luteal regulation in women? SUMMARY ANSWER: Prostaglandin E (PGE), which is produced in human granulosa-lutein cells stimulated with luteotropic hCG, exerts similar luteotropic effects to hCG, and the expression of PG synthetic and metabolic enzymes in the human CL is driven toward less PGE but more prostaglandin F (PGF) during luteolysis. WHAT IS KNOWN ALREADY: Uterine PGF is a major luteolysin in many non-primate species but not in women. Increases in the PGF synthase, aldo-ketoreductase family one member C3 (AKRIC3), have been observed in the CL of marmoset monkeys during luteolysis. PGE...

  12. Efficacy and safety of non-suture dural closure using a novel dural substitute consisting of polyglycolic acid felt and fibrin glue to prevent cerebrospinal fluid leakage : A non-controlled, open-label, multicenter clinical trial

    Terasaka, Shunsuke; Taoka, Toshiaki; Kuroda, Satoshi; Mikuni, Nobutaka; Nishi, Toru; Nakase, Hiroyuki; Fujii, Yukihiko; Hayashi, Yasuhiko; Murata, Jun-ichi; Kikuta, Ken-ichiro; Kuroiwa, Toshihiko; Shimokawa, Sachie; Houkin, Kiyohiro
    Objective: To evaluate the efficacy and safety of non-suture dural closure using a novel dural substitute (GM111) consisting of polyglycolic acid felt with a fibrin-glue-coated area commensurate in size with the dural defect. Methods: This was a non-controlled, open-label, multicenter clinical trial. The efficacy evaluation endpoints were 1) GM111's intra-operative capability to close dural defects and 2) prevention of cerebrospinal fluid (CSF) leakage and subcutaneous CSF retention throughout the postoperative period (evaluated by diagnostic imaging). Patients meeting the following three preoperative and two intra-operative selection criteria were enrolled: 1) between 12 and <75 years of age; 2) the dura is...

  13. Recurrent Pregnancy Loss and Cardiovascular Disease Mortality in Japanese Women : A Population-Based, Prospective Cohort Study

    Yamada, Keiko; Iso, Hiroyasu; Cui, Renzhe; Tamakoshi, Akiko
    Background: This study aimed to examine the association between recurrent pregnancy loss and the risk of cardiovascular disease mortality. Methods: We identified 54,652 women who were pregnant during the Japan Collaborative Cohort Study. These women were 40-79 years at the date of cohort entry between 1988 and 1990. Participants received municipal health screening examinations and completed self-administered questionnaires. The cause of death was confirmed by annual or biannual follow-up surveys for a median of 18 years. The exposure was the number of pregnancy loss. The outcome was mortality from total cardiovascular disease and its subtypes according to the International Classification...

  14. Blood soluble Fas levels and mortality from cardiovascular disease in middle-aged Japanese : The JACC study

    Iso, Hiroyasu; Maruyama, Koutatsu; Eshak, Ehab S.; Ikehara, Satoyo; Yamagishi, Kazumasa; Tamakoshi, Akiko
    Background and aims: Limited evidence has been available on the relationship between apoptosis and cardiovascular disease in population-based samples. We examined whether blood soluble Fas (sFas) are associated with mortality from cardiovascular diseases. Methods: In a nested case-control study under a large prospective cohort, the Japan Collaborative Cohort (JACC) Study, where a total of 39,242 subjects, 40-79 years of age, provided serum samples and were followed up for 9 years, we measured sFas levels among cases and controls, matched for sex, age, area of residence and year of serum storage. Conditional logistic regression models were used to calculate odds ratio...

  15. Passive smoking and chronic obstructive pulmonary disease mortality : findings from the Japan collaborative cohort study

    Ukawa, Shigekazu; Tamakoshi, Akiko; Yatsuya, Hiroshi; Yamagishi, Kazumasa; Ando, Masahiko; Iso, Hiroyasu
    Objectives: To elucidate the association between passive smoking at home and chronic obstructive pulmonary disease (COPD) mortality via a large-scale nationwide cohort study in Japan. Methods: Never smokers (n=34,604) aged 40-79 years at baseline (1988-1990; 4,884 men, 29,720 women) were included in the analysis. Passive smoking at home was measured based on self-reported frequency of weekly exposure to passive smoking at home. An inverse probability of treatment weighted competing risk model was used to calculate the hazard ratio (HR) and 95% confidence interval (CI) for COPD mortality. Results: During a median follow-up of 16.4 years, 33 participants (10 men, 23...

  16. Toll-like receptor 2 ligand and interferon-γ suppress anti-tumor T cell responses by enhancing the immunosuppressive activity of monocytic myeloid-derived suppressor cells

    Shime, Hiroaki; Maruyama, Akira; Yoshida, Sumito; Takeda, Yohei; Matsumoto, Misako; Seya, Tsukasa
    CD11b+Gr1+ myeloid-derived suppressor cells (MDSCs) suppress activation/proliferation of cytotoxic T cells, thereby hindering cancer immunotherapy. MDSCs are increased after adjuvant therapy with toll-like receptor (TLR) 2 ligands, such as Pam2CSK4, in tumor-bearing mice. However, it remains unknown if the activation of TLR2 in MDSCs affects their function and the therapeutic efficacy of TLR2 ligand. Here, we show that TLR2 signaling in CD11b+Ly6G-Ly6Chigh monocytic MDSCs (M-MDSCs), but not CD11b+Ly6G+Ly6Clow granulocytic MDSCs (G-MDSCs), enhances their immunosuppressive activity, thereby limiting anti-tumor T cell responses induced by TLR2-activated dendritic cells (DCs). iNOS induction was critical for Pam2CSK4-enhanced T cell suppression by M-MDSCs. iNOS was...

  17. Overexpression of perilipin1 protects against atheroma progression in apolipoprotein E knockout mice

    Yamamoto, Kohei; Miyoshi, Hideaki; Cho, Kyu Yong; Nakamura, Akinobu; Greenberg, Andrew S.; Atsumi, Tatsuya
    Background and aims: Perilipin1 (PLIN1), a lipid droplet-associated protein, plays an important role in the regulation of lipolysis and lipid storage in adipocytes. PLIN1 has recently been reported to be expressed in macrophages within atheroma plaques, suggesting PLIN1 may play a role in the accumulation of lipids at the arterial wall and in the development of atherosclerosis. To clarify the role of PLIN1 in the pathophysiology of atherosclerosis, we assessed the progression of atherosclerosis in PLIN1 transgenic mice (Plin1Tg). Methods: Plin1Tg were crossed with apolipoprotein E knockout mice (ApoeKO). C57BL/6J mice, ApoeKO and Plin1Tg/ApoeKO received a normal chow diet for...

  18. Pseudo-homozygous mutation due to a primer site polymorphism in hereditary ATTR amyloidosis : a pitfall of PCR-based genetic testing

    Shibata, Yuka; Matsushima, Masaaki; Yabe, Ichiro; Matsuda, Koichiro; Nagai, Azusa; Kano, Takahiro; Yamada, Takahiro; Sekijima, Yoshiki; Sasaki, Hidenao

  19. Impact of elemental diet on early recovery after laparoscopic colectomy : findings of a randomized controlled trial

    Shichinohe, Toshiaki; Sasaki, Takeshi; Kitashiro, Shuji; Morita, Takayuki; Ono, Koichi; Senmaru, Naoto; Ikeda, Junichi; Kojima, Tetsufumi; Kyogoku, Noriaki; Yamada, Hidehisa; Sato, Nagato; Kato, Kentaro; Murakami, Soichi; Ebihara, Yuma; Kurashima, Yo; Tamoto, Eiji; Noji, Takehiro; Nakamura, Toru; Okamura, Keisuke; Tsuchikawa, Takahiro; Hirano, Satoshi
    Purpose: An amino acid-containing elemental diet (ED) does not require digestion for nutritional absorption, making it a good option for patients with gastrointestinal malabsorption. We conducted a randomized trial to confirm that perioperative ED enhanced the recovery of patients undergoing laparoscopic colectomy. Methods: Patients in the intervention arm received commercially available ED from the day prior to surgery until postoperative day (POD) 3, whereas patients in the control group received a conventional perioperative diet program. To verify the endpoints, "estimated minimum length of stay in hospital after surgery" (emLOS) was defined as the number of days necessary to reach all...

  20. Altered balance of epidermis-related chemokines in epidermolysis bullosa

    Ujiie, Inkin; Fujita, Yasuyuki; Nakayama, Chihiro; Matsumura, Wakana; Suzuki, Shotaro; Shinkuma, Satoru; Nomura, Toshifumi; Abe, Riichiro; Shimizu, Hiroshi
    Background: Epidennolysis bullosa (EB) is a congenital, refractory skin disease and there are no fundamental treatments. Recently, allogenic cell therapies are beginning to be applied as potential treatments, that are based on the concept that the allogenic cells can migrate into the skin and reconstitute the skin components. Although the mechanisms of cell migration into skin are not fully understood, chemokines are regarded as key factors in recruiting bone marrow-derived cells. Objectives: Our study aims to elucidate the expression of chemokines in the EB patients. Methods: We determined the expression of wound-healing related chemokines in the sera, keratinocytes, and skin...

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