Recursos de colección

Hokkaido University Collection of Scholarly and Academic Papers (135.521 recursos)

HUSCAP (Hokkaido University Collection of Scholarly and Academic Papers) contains peer-reviewed journal articles, proceedings, educational resources and any kind of scholarly works of Hokkaido University.

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Mostrando recursos 1 - 20 de 145

  1. Morphology of the inferior border of the mandible: correct relation between the lowest position of the outer and inner contours of the cortex for a successful split

    Matsushita, Kazuhiro; Donen, Masaki; Harata, Saori; Nagamine, Kyosuke; Tei, Kanchu
    For a sagittal split ramus osteotomy to be secure, the relation between the outer and inner contours of the cortex at the inferior border of the mandible is critical. The lowest point of the outer contour is not always immediately below that of the inner contour, and the former is placed more lingually than the latter in about a third of all cases. This tendency is much more noticeable in skeletal class I and II malocclusions than class III. It is therefore important to examine the lowest point of the inferior border in every case, and to carry the inferior...

  2. Evaluation of Tissue Behavior on Three-dimensional Collagen Scaffold Coated with Carbon Nanotubes and β-tricalcium Phosphate Nanoparticles

    Miyaji, Hirofumi; Murakami, Shusuke; Nishida, Erika; Akasaka, Tsukasa; Fugetsu, Bunshi; Umeda, Junko; Kondoh, Katsuyoshi; Iizuka, Tadashi; Sugaya, Tsutomu
    We generated a three-dimensional collagen scaffold coated with carbon nanotubes (CNTs) and β-tricalcium phosphate (β-TCP) nanoparticles and histologically evaluated tissue behavior toward the nanomodified scaffold after subcutaneous tissue implantation in rat. Scanning electron microscopy images of the nanomodified scaffold showed that the collagen surface was enveloped by a meshwork of CNTs and dispersed β-TCP nanoparticles. Histological observations indicated that application of CNTs and β-TCP nanoparticles increased cell and blood vessel penetration into the collagen scaffold. CNTs consistently stimulated giant cell aggregation. In addition, CNTs and β-TCP application to the scaffold significantly promoted the DNA content of infiltrating cells and scaffold...

  3. Characterization and evaluation of graphene oxide scaffold for periodontal wound healing of class II furcation defects in dog

    Kawamoto, Kohei; Miyaji, Hirofumi; Nishida, Erika; Miyata, Saori; Kato, Akihito; Tateyama, Akito; Furihata, Tomokazu; Shitomi, Kanako; Iwanaga, Toshihiko; Sugaya, Tsutomu
    Introduction: The 3-dimensional scaffold plays a key role in volume and quality of repair tissue in periodontal tissue engineering therapy. We fabricated a novel 3D collagen scaffold containing carbon-based 2-dimensional layered material, named graphene oxide (GO). The aim of this study was to characterize and assess GO scaffold for periodontal tissue healing of class II furcation defects in dog. Materials and methods: GO scaffolds were prepared by coating the surface of a 3D collagen sponge scaffold with GO dispersion. Scaffolds were characterized using cytotoxicity and tissue reactivity tests. In addition, GO scaffold was implanted into dog class II furcation defects...

  4. Acinar cell response to liquid diet during rats' growth period differs in submandibular and sublingual glands from that in parotid glands

    Takahashi, Shigeru; Uekita, Hiroki; Taniwaki, Hiroto; Domon, Takanori
    Continuously feeding a liquid diet to growing rodents strongly inhibits parotid gland growth, due to suppressed growth of acinar cells. This study investigated whether a liquid diet had a similar effect on submandibular and sublingual glands of growing rats. Rats were weaned on day 21 after birth and then fed a pellet diet in the control group and a liquid diet in the experimental group for 0, 1, 2, 4, and 8 weeks. Their submandibular and sublingual glands were excised, weighed, and examined histologically, immunohistochemically (using antibodies to 5'-bromo-2-deoxyuridine and cleaved caspase 3), and ultra structurally. The submandibular glands did...

  5. Bilateral hypoplasia of the maxillary sinus : swelling of the nasal mucosa after periapical periodontitis

    Matsushita, Kazuhiro; Yamamoto, Hidekazu
    Maxillary sinus hypoplasia is a relatively rare condition and opportunities to encounter this pathology are scarce. We describe a bilateral case in a 64-year-old woman with swelling of the nasal floor mucosa resulting from periapical periodontitis in the condition of maxillary sinus hypoplasia, similar to the swelling of the sinus membrane in the condition of normal anatomical structure.

  6. Periodontal tissue repair after sealing of the gap in vertical root fracture

    Sugaya, Tsutomu; Tomita, Mahito; Motoki, Youji; Zaman, Khurshiduz; Miyaji, Hirofumi; Kawanami, Masamitsu
    The aim of this study was to determine whether sealing of fracture gap using adhesive resin through the root canal can prevent inflammation of periodontal tissue, and resealing the incompletely sealed fracture gap from outside can resolve such inflammation in experimentally created vertical root fractures. Vertical root fractures were created in incisor of beagles. In the experimental group, the fracture gap was sealed through the root canal with adhesive resin. After 5 weeks, sites with the clinical attachment level >= 4 mm were further divided randomly into the poor-replanting group and the poor-untreated group. In the poor-replanting group, the tooth...

  7. Movement-related cortical magnetic fields associated with self-paced tongue protrusion in humans

    Maezawa, Hitoshi; Oguma, Hidetoshi; Hirai, Yoshiyuki; Hisadome, Kazunari; Shiraishi, Hideaki; Funahashi, Makoto
    Sophisticated tongue movements are coordinated finely via cortical control. We elucidated the cortical processes associated with voluntary tongue movement. Movement-related cortical fields were investigated during self-paced repetitive tongue protrusion. Surface tongue electromyograms were recorded to determine movement onset. To identify the location of the primary somatosensory cortex (S1), tongue somatosensory evoked fields were measured. The readiness fields (RFs) over both hemispheres began prior to movement onset and culminated in the motor fields (MFs) around movement onset. These signals were followed by transient movement evoked fields (MEFs) after movement onset. The MF and MEF peak latencies and magnitudes were not different...

  8. Bone Induction by α-tricalcium Phosphate Microparticle Emulsion Containing Simvastatin

    Tateyama, Akito; Kato, Akihito; Miyaji, Hirofumi; Nishida, Erika; Iwasaki, Yasuhiro; Fujii, Syuji; Kawamoto, Kohei; Shitomi, Kanako; Furihata, Tomokazu; Mayumi, Kayoko; Sugaya, Tsutomu
    To improve the degradability and operability of conventional bone graft materials, we fabricated a water-oil emulsion based on α-tricalcium phosphate (α-TCP) bone paste. Simvastatin, a lipophilic hyperlipidemia treatment agent, reportedly enhances the expression of bone morphogenetic protein-2 and subsequent bone formation. Accordingly, we assessed the bone forming effects of α-TCP bone-paste containing simvastatin in rat cranial bone defects. Bone paste exhibited porous structure and generation of hydroxyapatite after solidification. X-ray image analysis and histological examination were carried out after implantation of bone paste into rat skull defect. The results showed that new bone was formed after implantation of bone paste containing...

  9. Near-infrared Irradiation and Graphene Oxide Film Fabricated on Dentin Surface Exhibit Photothermal and Antibacterial Effects

    Nagao, Keishi; Miyaji, Hirofumi; Nishida, Erika; Akasaka, Tsukasa; Miyata, Saori; Shitomi, Kanako; Mayumi, Kayoko; Kato, Akihito; Sugaya, Tsutomu
    Background and objectives: Graphene oxide (GO) is a monolayer sheet of carbon with a thickness of 1 nm or less. Recent studies have revealed that GO exerts antibacterial properties, absorbs near-infrared (NIR) irradiation and generates heat. In this study, we fabricated a GO film on a human dentin block and investigated the photothermal and antibacterial effects of GO and NIR irradiation against Streptococcus mutans. Methods: The dentin block was immersed in GO dispersion (concentration: 0, 1 and 10 μg/mL). GO-coated dentin blocks were observed using scanning electron microscopy (SEM) and characterized using the dentinal tubule sealing score. The temperature increase of...

  10. Activation of nucleotide-binding domain-like receptor containing protein 3 inflammasome in dendritic cells and macrophages by Streptococcus sanguinis

    Saeki, Ayumi; Suzuki, Toshihiko; Hasebe, Akira; Kamezaki, Ryousuke; Fujita, Mari; Nakazawa, Futoshi; Shibata, Ken-Ichiro
    Streptococcus sanguinis is frequently isolated from the blood of patients with infective endocarditis and contributes to the pathology of this disease through induction of interleukin (IL)-1β responsible for the development of the disease. However, the mechanism of IL-1β induction remains unknown. In this study, S. sanguinis activated a murine dendritic cell (DC) to induce IL-1β and this activity was attenuated by silencing the mRNAs of nucleotide-binding domain-like receptor containing protein 3 (NLRP3) and caspase-1. S. sanguinis induced IL-1β production in murine bone marrow-derived macrophage (BMM), but this activity was significantly reduced in BMMs from NLRP3-, apoptosis-associated speck-like protein containing a caspase-recruitment domain-, and caspase-1-deficient mice. DC phagocytosed S. sanguinis cells,...

  11. Inhibition of neuropeptide Y Y1 receptor induces osteoblast differentiation in MC3T3-E1 cells

    Yahara, Motoki; Tei, Kanchu; Tamura, Masato
    Neuropeptide Y (NPY) is a major neural signaling molecule. NPY is produced by peripheral tissues, such as osteoblasts, and binds to the corresponding Y1 receptor that belongs to the G-protein-coupled receptor family. Osteoblast-specific Y1 receptor knockout mice exhibit high bone mass, indicating a role of the NPY-Y1 receptor axis in the regulation of bone homeostasis. In the bone microenvironment, peripheral nerve fibers and osteoblasts produce NPY. However, the effects of the Y1 receptor on osteoblasts remain unexplored. In the present study, an RNA interference approach was employed to target the Y1 receptor, in order to determine whether it may function...

  12. Biological application of focus ion beam-scanning electron microscopy (FIB-SEM) to the imaging of cartilaginous fibrils and osteoblastic cytoplasmic processes

    Hasegawa, Tomoka; Endo, Takashi; Tsuchiya, Erika; Kudo, Ai; Zhao, Shen; Moritani, Yasuhito; Abe, Miki; Yamamoto, Tomomaya; Hongo, Hiromi; Tsuboi, Kanako; Yoshida, Taiji; Nagai, Tomoya; Khadiza, Naznin; Yokoyama, Ayako; Luiz de Freitas, Paulo Henrique; Li, Minqi; Amizuka, Norio
    Objectives: The aim of this study is a biological application of focused ion beam-scanning electron microscopy (FIB-SEM) to demonstrate serial sectional images of skeletal tissues, here presenting the ultrastructure of 1) cartilaginous extracellular fibrils and 2) osteoblastic cytoplasmic processes. Methods: Seven weeks-old female wild-type mice were fixed with half-Karnovsky solution and subsequent OsO4, and the tibiae were extracted for block staining prior to observation under transmission electron microscope (TEM) and FIB-SEM. Results: TEM showed the fine fibrillar, but somewhat amorphous ultrastructure of the intercolumnar septa in the growth plate cartilage. Alternatively, FIB-SEM revealed bundles of stout fibrils at regular intervals paralleling the...

  13. Periodontal tissue engineering by nano beta-tricalcium phosphate scaffold and fibroblast growth factor-2 in one-wall infrabony defects of dogs

    Ogawa, Kosuke; Miyaji, Hirofumi; Kato, Akihito; Kosen, Yuta; Momose, Takehito; Yoshida, Takashi; Nishida, Erika; Miyata, Saori; Murakami, Shusuke; Takita, Hiroko; Fugetsu, Bunshi; Sugaya, Tsutomu; Kawanami, Masamitsu
    Background and Objective: Nanoparticle bioceramics are being investigated for biomedical applications. We fabricated a regenerative scaffold comprising type I collagen and beta-tricalcium phosphate (β-TCP) nanoparticles. Fibroblast growth factor-2 (FGF-2) is a bioeffective signaling molecule that stimulates cell proliferation and wound healing. This study examined the effects on bioactivity from a nano-β-TCP/collagen scaffold loaded with FGF-2, particularly on periodontal tissue wound healing. Material and Methods: β-TCP was pulverized into nano-sized particles (84 nm) and was then dispersed. Nano-β-TCP scaffold was prepared by coating the surface of a collagen scaffold with a nano-sized β-TCP dispersion. Scaffolds were characterized using scanning electron microscopy (SEM), compressive testing,...

  14. Dose effects of beta-tricalcium phosphate nanoparticles on biocompatibility and bone conductive ability of three-dimensional collagen scaffolds

    Murakami, Shusuke; Miyaji, Hirofumi; Nishida, Erika; Kawamoto, Kohei; Miyata, Saori; Takita, Hiroko; Akasaka, Tsukasa; Fugetsu, Bunshi; Iwanaga, Toshihiko; Hongo, Hiromi; Amizuka, Norio; Sugaya, Tsutomu; Kawanami, Masamitsu
    Three-dimensional collagen scaffolds coated with beta-tricalcium phosphate (β-TCP) nanoparticles reportedly exhibit good bioactivity and biodegradability. Dose effects of β-TCP nanoparticles on biocompatibility and bone forming ability were then examined. Collagen scaffold was applied with 1, 5, 10, and 25 wt% β-TCP nanoparticle dispersion and designated TCP1, TCP5, TCP10, and TCP25, respectively. Compressive strength, calcium ion release and enzyme resistance of scaffolds with β-TCP nanoparticles applied increased with β-TCP dose. TCP5 showed excellent cell-ingrowth behavior in rat subcutaneous tissue. When TCP10 was applied, osteoblastic cell proliferation and rat cranial bone augmentation were greater than for any other scaffold. The bone area...

  15. How much medium do you use for cell culture? Medium volume influences mineralization and osteoclastogenesis in vitro

    Yoshimura, Yoshitaka; Kikuiri, Takashi; Hasegawa, Tomokazu; Matsuno, Mino; Minamikawa, Hajime; Deyama, Yoshiaki; Suzuki, Kuniaki
    Bone is maintained by a balance between bone formation and resorption. This remodeling is controlled by a wide variety of systemic and local factors including hormones, cytokines and mechanical stresses. The present in vitro study examined the impact of medium volume, using 0.4, 0.6, 0.8, 1.0, 1.5 and 2.0 ml/well in a 24-well plate, on the differentiation of osteoblasts and osteoclasts. There were no differences in the alkaline phosphatase activity of osteoblasts amongst the groups; however, the area of mineral deposition was decreased in a media volume-dependent manner. A co-culture of osteoblastic cells with bone marrow cells revealed a reduction...

  16. Evaluation of the maxillofacial morphological characteristics of Apert syndrome infants

    Kakutani, Hitomi; Sato, Yoshiaki; Tsukamoto-Takakusagi, Yuri; Saito, Fumio; Oyama, Akihiko; Iida, Junichiro
    Apert syndrome is a rare craniosynostosis syndrome characterized by irregular craniosynostosis, midface hypoplasia, and syndactyly of the hands and feet. Previous studies analyzed individuals with Apert syndrome and reported some facial and intraoral features caused by severe maxillary hypoplasia. However, these studies were performed by analyzing both individuals who had and those had not received a palate repair surgery, which had a high impact on the maxillary growth and occlusion. To highlight the intrinsic facial and intraoral features of Apert syndrome, five Japanese individuals with Apert syndrome from 5 years and 2 months to 9 years and 10 months without...

  17. Antibacterial and cytotoxic effects of photoexcited Au clusters via blue high-power or white low-power light emitting diode irradiation

    Miyata, Saori; Miyaji, Hirofumi; Kawasaki, Hideya; Nishida, Erika; Shitomi, Kanako; Akasaka, Tsukasa; Tanaka, Saori; Iizuka, Tadashi; Sugaya, Tsutomu
    The development of photosensitizers and light sources has enabled the use of antimicrobial photodynamic therapy (aPDT) in various dental therapies. In the present study, we compared the antibacterial and cytotoxic effects of Au clusters photoexcited by blue and white LED irradiation. We fabricated novel photosensitizers, captopril-protected gold (Capt-Au) clusters and lysozyme-stabilized gold (Lyz-Au) clusters, for aPDT. Au clusters were then photoexcited by two kinds of light sources, blue high-power and white low-power light-emitting diodes (LEDs). Since white LED contains a wide spectrum of light (400–750 nm), white LED would be relevant for aPDT even if using a low-power source. The turbidity and viability of Streptococcus...

  18. Influence of enamel matrix derivative on healing of root surfaces after bonding treatment and intentional replantation of vertically fractured roots

    Sugaya, Tsutomu; Tomita, Mahito; Motoki, Youji; Miyaji, Hirofumi; Kawamami, Masamitsu
    Background/Aim: The objectives of this study were to histopathologically evaluate cementum regeneration on root surfaces when an enamel matrix derivative (EMD) was used to bond a vertically fractured root and to evaluate the effectiveness of EMD in inhibiting root resorption. Material and methods: A total of 40 roots from 24 maxillary premolars in beagles were used. The root was vertically fractured using a chisel and mallet. Super-Bond was then used to bond the fractured root. In the experimental group, the root surface was treated with ethylenediaminetetraacetic acid and an EMD. The control group received no treatment. The root was then...

  19. Influence of enamel matrix derivative on healing of root surfaces after bonding treatment and intentional replantation of vertically fractured roots

    Sugaya, Tsutomu; Tomita, Mahito; Motoki, Youji; Miyaji, Hirofumi; Kawamami, Masamitsu
    Background/Aim: The objectives of this study were to histopathologically evaluate cementum regeneration on root surfaces when an enamel matrix derivative (EMD) was used to bond a vertically fractured root and to evaluate the effectiveness of EMD in inhibiting root resorption. Material and methods: A total of 40 roots from 24 maxillary premolars in beagles were used. The root was vertically fractured using a chisel and mallet. Super-Bond was then used to bond the fractured root. In the experimental group, the root surface was treated with ethylenediaminetetraacetic acid and an EMD. The control group received no treatment. The root was then...

  20. Study of training for improving lip incompetence

    Yoshizawa, Saori; Ohtsuka, Mai; Kaneko, Tomoo; Iida, Junichiro
    Purpose: We have been using myofunctional therapy in orthodontic treatment to improve orofacial disorders. Our previous study showed that lip training increased orbicularis oris muscle strength and endurance. The aim of this study was to determine the effectiveness of hypoxic lip training for improving lip incompetence. Subjects and methods: Twenty healthy subjects (10 males and 10 females, 23.6 +/- 2.3 years old) with lip incompetence participated in this study. We recorded the sealed lip ratio calculated by using the formula "(lip-sealingtime/totalrecorded time) x 100" during relaxation (listening to soothing music) and during concentration (performing a mathematical calculation). Then the subjects...

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