Hokkaido University confered the degree of Doctor of Philosophy on December 24, 2010 to 4
Yamanaka, Atsushi; Yamauchi, Kiyoshi; Tsujimoto, Tsunenori; Mizoguchi, Toshio; Oi, Toru; Sawada, Seigo; Shimozuru, Michito; Tsubota, Toshio
In order to develop a method for estimating the success/failure rates of reproductive
processes, especially those of ovulation and neonate nurturing, in the Japanese black bear
(Ursus thibetanus japonicus), we examined offspring status, corpora lutea (CLs), placental
scars (PSs) and corpora albicantia (CAs) in 159 females (0-23 years old) killed as nuisances
on Honshu Island of Japan during 2001-2009. PSs were found to remain in the uterus at
least until November of the year of parturition. CA detectability began to decline after
September of the year of parturition. Monthly and age-specific proportions of CL-present
females revealed that the post-mating season starts in August, and that the age...
Asmah Abdul Samad, Rozanah; Nomura, Naoki; Tsuda, Yoshimi; Manzoor, Rashid; Kajihara, Masahiro; Tomabechi, Daisuke; Sasaki, Takashi; Kokumai, Norihide; Ohgitani, Toshiaki; Okamatsu, Masatoshi; Takada, Ayato; Sakoda, Yoshihiro; Kida, Hiroshi
Inactivated influenza virus vaccine prepared from a non-pathogenic influenza virus strain
A/duck/Hokkaido/Vac-1/2004 (H5N1) from the virus library conferred protective immunity
to chickens against the challenge of antigenically drifted highly pathogenic avian influenza
virus (HPAIV), A/whooper swan/Hokkaido/1/2008 (H5N1). The efficacy of the vaccine was
comparable to that prepared from genetically modified HPAIV strain ΔRRRRK rg-A/
whooper swan/Mongolia/3/2005 (H5N1), which is more antigenically related to the challenge
virus strain, in chickens.
Asmah Abdul Samad, Rozanah; Sakoda, Yoshihiro; Tsuda, Yoshimi; Simulundu, Edgar; Manzoor, Rashid; Okamatsu, Masatoshi; Ito, Kimihito; Kida, Hiroshi
Recent introduction of H5N1 highly pathogenic avian influenza virus (HPAIV) in wild birds
from poultry in Eurasia signaled the possibility that this virus may perpetuate in nature.
Surveillance of avian influenza especially in migratory birds, therefore, has been conducted
to provide information on the viruses brought by them to Hokkaido, Japan, from their
nesting lakes in Siberia in autumn. During 2008-2009, 62 influenza viruses of 21 different
combinations of hemagglutinin (HA) and neuraminidase (NA) subtypes were isolated. Up to
September 2010, no HPAIV has been found, indicating that H5N1 HPAIV has not
perpetuated at least dominantly in the lakes where ducks nest in summer in Siberia. The
Moustafa, Amira; Sakamoto, Kentaro Q; Habara, Yoshiaki
In an attempt to explore the functioning of nitric oxide (NO) in pancreatic exocrine cells,
we have recently obtained several lines of circumstantial evidence indicating that one of
molecular targets of NO is phospholipase C (PLC), the activation of which leads to an
increase in the cytosolic Ca2＋ concentration ([Ca2＋]i) via inositol 1, 4, 5-trisphosphate, IP3.
However, whether IP3 is actually produced by NO has not yet been substantiated. The
present study was therefore designed to directly measure the intracellular IP3
concentration ([IP3]i) for better understanding of the underlying mechanisms with the help
of pharmacological tools. [IP3]i was measured using a fluorescence polarization technique
(HitHunter™). We obtained the...