ORBi Open Repository and Bibliography
In may 2007, the ULg's Administrative Board (joined in June 2007 by the FUSAGx) decided to create an institutional repository and defined a strong institutional self-archiving policy to increase the visibility, accessibility and impact of the University's publications (Board's decision).
This decision led to the official launch, in November 2008, of the ORBi platform including both the Academic Bibliography and the Institutional Repository of the Wallonia-Europe University Academy.
Etude de la reconstitution immunitaire après greffe de cellules souches hématopoïétiques : focus sur la fonction thymique et les lymphocytes T régulateurs - HANNON, Muriel
L’allogre e de cellules souches hématopoïétiques, après conditionnement myéloalbatif
ou non, est devenue une option de choix dans le traitement des maladies hématologiques
malignes ou génétiques. Malheureusement le succès de ce traitement se trouve trop souvent
entaché par des complications majeures telles que les infections ou la maladie du greffon
contre l’hôte (GVHD), dans laquelle les cellules du donneur attaquent l’organisme du
receveur. La reconstitution d’un système immunitaire fonctionnel, notamment via le thymus,
est une étape clef dans la résolution de ces deux complications et l’utilisation de lymphocytes
T régulateurs semble très prometteuse dans la lutte contre la GVHD. Le présent travail vise
donc à étudier la reconstitution...
Azacytidine augments regulatory T cells and prevents experimental xenogeneic graft-versus-host disease. - Ehx, Grégory
The demethylating agent 5-azacytidine (AZA) has proven its efficiency in myeloid malignancies treatment. Recently, several studies have shown that AZA also presents an immunomodulatory activity, mainly through the induction of regulatory T cells (Treg) differentiation from conventional T cells. Treg promotion is a promising treatment option for graft-versus-host disease (GVHD) following hematopoietic stem cell transplantation. In this way, AZA has been shown to reduce GVHD in mouse-to-mouse transplantation models. However, the impact of AZA on human GVHD remains elusive as well as its effects on human Treg and on the other T cell subsets in vivo. Here we report AZA...
AIRE polymorphism, melanoma-antigen-specific T cell immunity, and susceptibility to melanoma. - Conteduca, Giuseppina
AIRE which regulates T cell repertoire is involved in susceptibility to melanoma. Whether this role is mediated by melanoma antigen (MA)-specific T cell immunity is not known. In this study, we have tested this possibility, taking advantage of the structural and functional homology of human AIRE and MAGE with their mouse counterparts. AIRE and MAGEB2 expression was measured by real time PCR in medullary thymic epithelial cells (mTECs) from two strains of C57BL/6 mice bearing selectively one of the two (T or C) allelic variants of rs1800522 AIRE SNP. The extent of apoptosis induced by mTECs in MAGEB2-specific T cells...
AIRE polymorphisms induce variable extent of apoptosis on melanoma-specific CD8+ T lymphocytes. - Conteduca, Giuseppina
AIRE regulates thymocyte selection by inducing the expression of tissue-restricted self antigens (TRAs) in medullary thymic epithelial cells (mTECs). MAGE antigens are among TRAs regulated by AIRE. Single nucleotide polymorphisms (SNPs) of AIRE gene have been reported in humans but their impact on repertoire selection of tumor antigen-specific T lymphocytes is unknown. We report here that the rs1800522 SNP of human AIRE gene is present in mice and that the relative T or C allelic variants differently regulate MAGEB2 gene expression in mTECs. The C allelic variant, protective in humans against melanoma, induces lower MAGEB2 expression than the T allele....
Aire controls the differentiation program of thymic epithelial cells in the medulla for the establishment of self-tolerance
The roles of autoimmune regulator (Aire) in the expression of the diverse arrays of tissue-restricted antigen (TRA) genes from thymic epithelial cells in the medulla (medullary thymic epithelial cells [mTECs]) and in organization of the thymic microenvironment are enigmatic. We approached this issue by creating a mouse strain in which the coding sequence of green fluorescent protein (GFP) was inserted into the Aire locus in a manner allowing concomitant disruption of functional Aire protein expression. We found that Aire+ (i.e., GFP+) mTECs were the major cell types responsible for the expression of Aire-dependent TRA genes such as insulin 2 and...