ORBi Open Repository and Bibliography
In may 2007, the ULg's Administrative Board (joined in June 2007 by the FUSAGx) decided to create an institutional repository and defined a strong institutional self-archiving policy to increase the visibility, accessibility and impact of the University's publications (Board's decision).
This decision led to the official launch, in November 2008, of the ORBi platform including both the Academic Bibliography and the Institutional Repository of the Wallonia-Europe University Academy.
Cord blood banking - Brand, A.; Rebulla, P.; Engelfriet, C. P.; Reesink, H. W.; Beguin, Yves; Baudoux, Etienne; Kögler, G.; Ebrahimi, M.; Grazzini, G.; Costa, A. N.; Bosi, A.; Sacchi, S.; Lombardini, L.; Pupella, S.; Lecchi, L.; Calderon Garciduenas, E. D.; van Beckhoven, J. M.; de Wit, H. J. C.; Fibbe, W. E.; Zhiburt, E. B.; Bart, T.; Beksac, M.; Navarrete, C.; Regan, F.
Matrix Metalloproteinase-9 gene induction by a truncated oncogenic NF-κB2 protein involves the recruitment of MLL1 and MLL2 H3K4 histone methyltransferase complexes. - Robert, Isabelle; Aussems, Marie; Keutgens, Aurore; Zhang-Shao, Xin; Hennuy, Benoît; Viatour, Patrick; Vanstraelen, Gaetan; Merville, Marie-Paule; Chapelle, Jean-Paul; de Leval, Laurence; Lambert, Frédéric; Dejardin, Emmanuel; Gothot, André; Chariot, Alain
Constitutive nuclear factor (NF)-kappaB activation in haematological malignancies is caused in several cases by loss of function mutations within the coding sequence of NF-kappaB inhibitory molecules such as IkappaBalpha or p100. Hut-78, a truncated form of p100, constitutively generates p52 and contributes to the development of T-cell lymphomas but the molecular mechanism underlying this oncogenic potential remains unclear. We show here that MMP9 gene expression is induced through the alternative NF-kappaB-activating pathway in fibroblasts and also on Hut-78 or p52 overexpression in fibroblasts as well as in lymphoma cells. p52 is critical for Hut-78-mediated MMP9 gene induction as a Hut-78...
Hemorrhage, impaired hematopoiesis, and lethality in mouse embryos carrying a targeted disruption of the Fli1 transcription factor. - Spyropoulos, Demetri D.; Pharr, Pamela N.; Lavenburg, Kim R.; Jackers, Pascale; Papas, Takis S.; Ogawa, Makio; Watson, Dennis K.
The Ets family of transcription factors have been suggested to function as key regulators of hematopoeisis. Here we describe aberrant hematopoeisis and hemorrhaging in mouse embryos homozygous for a targeted disruption in the Ets family member, Fli1. Mutant embryos are found to hemorrhage from the dorsal aorta to the lumen of the neural tube and ventricles of the brain (hematorrhachis) on embryonic day 11.0 (E11.0) and are dead by E12.5. Histological examinations and in situ hybridization reveal disorganization of columnar epithelium and the presence of hematomas within the neuroepithelium and disruption of the basement membrane lying between this and mesenchymal...
Ets-dependent regulation of target gene expression during megakaryopoiesis - Jackers, Pascale; Szalai, Gabor; Moussa, Omar; Watson, Dennis K
Megakaryopoiesis is the process by which hematopoietic stem cells in the bone marrow differentiate into mature megakaryocytes. The expression of megakaryocytic genes during megakaryopoiesis is controlled by specific transcription factors. Fli-1 and GATA-1 transcription factors are required for development of megakaryocytes and promoter analysis has defined in vitro functional binding sites for these factors in several megakaryocytic genes, including GPIIb, GPIX, and C-MPL. Herein, we utilize chromatin immunoprecipitation to examine the presence of Ets-1, Fli-1, and GATA-1 on these promoters in vivo. Fli-1 and Ets-1 occupy the promoters of GPIIb, GPIX, and C-MPL genes in both Meg-01 and CMK11-5 cells....
Selective defect of anti-pneumococcal IgG in a patient with persistent polyclonal B cell lymphocytosis. - Hafraoui, Kaoutar; Moutschen, Michel; Smet, Julie; Mascart, Francoise; Schaaf-Lafontaine, Nicole; Fillet, Georges
BACKGROUND: Persistent polyclonal B cell lymphocytosis (PPBL) is a rare condition characterized by increased IgM and large excess of B cells with an IgD(+) CD27(+) phenotype. In normal individuals, these cells play a central role in the defense against pneumococcal infection. So far, few studies have characterized humoral immune responses in PPBL patients. We therefore measured IgG directed against S. pneumoniae antigens in a 51 yr-old woman with PPBL before and after vaccination with a pneumococcal 23-valent polysaccharide vaccine. METHODS: Antibodies against pneumococcal antigens were measured first with an overall immunoassay using microplates coated with the 23-valent pneumococcal vaccine. A...
Allogeneic hematopoietic cell transplantation as treatment for hematological malignancies: a review. - Baron, Frédéric; Storb, Rainer
Allogeneic hematopoietic cell transplantation (HCT) was originally developed as a form of rescue from high-dose chemoradiotherapy, which is given both to eradicate malignancy and provide sufficient immunosuppression for allogeneic engraftment. The first attempts of allogeneic HCT in humans met with little success. However, a better understanding of the complexities of the human leukocyte antigen (HLA) system has allowed selecting compatible sibling donors, and the development of postgrafting immunosuppressive regimens has helped prevent serious graft-versus-host disease, thereby changing the role of allogeneic HCT from a desperate therapeutic maneuver to a curative treatment modality for many patients with malignant hematological diseases. In...
Comorbidity and disease status based risk stratification of outcomes among patients with acute myeloid leukemia or myelodysplasia receiving allogeneic hematopoietic cell transplantation. - Sorror, Mohamed L; Sandmaier, Brenda M; Storer, Barry E; Maris, Michael B; Baron, Frédéric; Maloney, David G; Scott, Bart L; Deeg, H Joachim; Appelbaum, Frederick R; Storb, Rainer
PURPOSE: Retrospective studies have shown similar survival among patients with acute myeloid leukemia (AML) and myelodysplasia (MDS) after nonmyeloablative compared with myeloablative conditioning. Refined risk stratification is required to design prospective trials. PATIENTS AND METHODS: We stratified outcomes among patients with AML (n = 391) or MDS (n = 186) who received either nonmyeloablative (n = 125) or myeloablative (n = 452) allogeneic hematopoietic cell transplantation (HCT) based on comorbidities, as assessed by a HCT-specific comorbidity index (HCT-CI), as well as disease status. Patients receiving nonmyeloablative conditioning were older, more frequently pretreated, more often received unrelated grafts, and more often...
Les cellules souches mesenchymateuses: une nouvelle therapeutique polyvalente. - Baron, Frédéric; Gothot, André
Mesenchymal stem cells (MSC) reside in the stromal compartment of the hematopoietic bone marrow. Although present in small numbers in vivo, MSC may be easily isolated and expanded in cell culture. MSC are able to generate bone, cartilage, fat, and under specific conditions, liver, muscle and nerve. Numerous studies have suggested a potential use of MSC to repair degenerative or traumatic lesions, in organs where tissue repair is limited. Furthermore, MSC are endowed with immunosuppressive properties, utilized to control graft versus host disease and rejection of allogenic hematopoietic stem cell transplants.
Intensified postgrafting immunosuppression failed to assure long-term engraftment of dog leukocyte antigen-identical canine marrow grafts after 1 gray total body irradiation. - Sorror, Mohamed L; Leisenring, Wendy; Mielcarek, Marco; Baron, Frédéric; Diaconescu, Razvan; Hogan, William J; Graves, Scott S; Storb, Rainer
BACKGROUND: Late graft rejection after conditioning with 1 Gy of total body irradiation (TBI) was consistently seen in historical dogs given two postgrafting immunosuppressive drugs. METHODS: Here, 16 dogs were given four different three-drug combinations of cyclosporine, mycophenolate mofetil, sirolimus, or methotrexate after 1 Gy TBI and dog leukocyte antigen-identical marrow grafts. In addition, we assessed the effects of TBI doses of 0.5, 1.0, 2.0, or 3.0 Gy, respectively, on immune functions in six dogs not given marrow grafts. RESULTS: All dogs showed initial engraftment, 13 rejected, and three had sustained grafts beyond 26 weeks. The dogs with durable grafts...
What is the role for donor natural killer cells after nonmyeloablative conditioning? - Baron, Frédéric; Petersdorf, Effie W; Gooley, Ted; Sandmaier, Brenda M; Malkki, Mari; Chauncey, Thomas R; Maloney, David G; Storb, Rainer
We investigated the impacts of the tempo of early (days 14, 28, and 42) donor T cell and natural killer (NK) cell engraftment, missing recipient killer cell immunoglobulin-like receptor (KIR) ligands, and numbers of donor inhibitory and activating KIR genes on hematopoietic cell transplantation (HCT) outcomes in 282 patients with hematologic malignancies given nonmyeloablative conditioning. Modeling chimerism levels as a continuous linear variable, we found that high early donor T cell chimerism was significantly associated with acute graft-versus-host disease (aGVHD) (P = .01), whereas high donor NK cell chimerism levels had no such association (P = .38). Conversely, high donor...
A quantitative study of peripheral blood stem cell contamination in diffuse large-cell non-Hodgkin's lymphoma: one-half of patients significantly mobilize malignant cells. - Jacquy, C; Soree, A; Lambert, Frédéric; Bosly, A; Ferrant, A; Andre, Marie-Elizabeth; Parma, J; Kentos, A; Martiat, Philippe
Autologous transplantation using peripheral blood stem cells (PBSCs) collected after chemotherapy, followed by growth factor administration (ASCT), is increasingly used in the treatment of non-Hodgkin's lymphoma (NHL). However, quantitative data regarding contaminating malignant cells in the harvests are still scarce. We prospectively investigated 37 diffuse large-cell lymphomas (DLCLs) in complete remission (CR) that were treated according to multicentric protocols at our centre. DNA was extracted from the diagnostic lymph node. The complementarity-determining region (CDR) III was sequenced and a patient-specific oligomer synthesized. Contamination was evaluated semiquantitatively by polymerase chain reaction (PCR) and was confirmed by a limiting dilution analysis. PBSCs...
Peripheral blood stem cell contamination in mantle cell non-Hodgkin lymphoma: the case for purging? - Jacquy, Caroline; Lambert, Frédéric; Soree, A; Van Daele, S; Heusterspreute, M; Bosly, A; Ferrant, A; Parma, J; Bron, D; Martiat, P
Intensification using peripheral blood stem cells collected after chemotherapy followed by growth factors is being increasingly investigated as an alternative to conventional chemotherapy for mantle cell non-Hodgkin lymphoma. We investigated 14 grades III-IV, t(11;14)-positive cases for contamination of PBSC collected after a polychemotherapy regimen followed by G-CSF. Patients were first treated with a polychemotherapy regimen. There were four CR, seven PR, two refractory and one early death. Seven patients have been transplanted, in whom PBSC were mobilized, using either cyclophosphamide/VP16 or Dexa-BEAM followed by G-CSF. For all patients, whether actually autografted or not, PB cells were tested at the time...
Molecular classification of T-cell lymphomas. - De Leval, Laurence; Bisig, Bettina; Thielen, Caroline; Boniver, Jacques; Gaulard, P
T-cell neoplasms encompass a heterogeneous group of relatively rare disease entities. This review, focused on lymphoblastic tumors (T-ALL/LBL) and nodal-based peripheral T-cell lymphomas (PTCL), summarizes recent advances in the molecular characterization of these diseases. In T-ALL/LBL, molecular subgroups delineated by gene expression profiling correlate with leukemic arrest at specific stages of normal thymocyte development and different oncogenic pathways, and seem to be of interest for prognosis prediction. Angioimmunoblastic T-cell lymphoma (AITL), one of the most common PTCL entities, comprises neoplastic cells with a molecular signature similar to normal follicular helper T cells, and this cellular derivation might account for several...