Coucke, Philippe [author]; Lin-Quan, Sun [author]; Vogel, Charles-André [author]; Mirimanoff, René-Olivier [author]; COUCKE, Philippe [author]; Slosman, D-O [author]; Buchegger, F [author]
Timing effects of radioimmunotherapy (HIT) combined with external
beam radiotherapy (RT) were assessed In human colon carcinoma xe
nografts. Initially, dose effects offractlonated RT and RIT were evaluated
separately. Then, 30 Gy RT (10 fractions over 12 days) were combined
with three weekly Lv. injections of 200 g@Ci of 131I-labeled anti-carcino
embryonic antigen monoclonal antibodies in four different treatment
schedules. RIT was given either prior to, concurrently, Immediately after,
or 2 weeks after RT administration. The longest regrowth delay (RD) of
105 days was observed in mice treated by concurrent administration of
RT and lilT, whereas the RDs of RT and RIT alone were 34 and 20 days,
Coucke, Philippe [author]; Delaloye, J-F [author]; Pampallona, S [author]; Peltecu, G [author]; De Grandi, P [author]
of the cervix treated by radical radiation therapy. Method; Three hundred and sixty patients with FIG0 stage IB-IIIB
carcinoma of the cervix were treated in Lausanne (Switzerland) with external radiation and brachytherapy as first line
therapy. Median therapy duration was 45 days. Patients were classified according to the duration of the therapies,
taking 60 days (the 75th percentile) as an arbitrary cut-off. Results: The 5-year survival was 61% (SE. = 3%) for the
therapy duration group of less than 60 days and 53% (SE. = 7%) for the group of more than 60 days. In terms of
univariate hazard ratio (HR), the relative difference between...
Tercier, P-A [author]; COUCKE, Philippe [author]; Fankhauser, H [author]; Mirimanoff, R-O [author]; Uske, A [author]; Valley, J-F [author]
Background and purpose: This work was undertaken to improve the definition of target volumes for radiosurgery using the angiographic and CT data.
Materials and methods: The basis of this new method is to combine both imaging modalities and to compare them in each representation, i.e. to plot the volume obtained by angiography on CT images and also the contours defined by the CT on angiographic films. To obtain the angiographic volume, the radiographs are taken at several incidence angles. The X-ray sources position and the position of the films are determined using rectangular markers, then the intersection of all the loci...
Rosset, A [author]; Zografos, L [author]; COUCKE, Philippe [author]; Monney, M [author]; Mirimanoff, R-O [author]
Purpose: This retrospective study was undertaken to clarify the role of high energy external beam radiation therapy (EBRT) and to
determine its safety and efficacy on local control and visual acuity in patients suffering from choroidal metastases (CM).
Materials and methods: The records of 58 consecutive patients treated with EBRT between 1970 and 1993 were analyzed. The female to
male ratio was 2.9 and the median age was 59 years (range 40–81 years). Thirty-six patients (62%) had unilateral CM and 22 patients had
bilateral CM. The mean number of lesions per eye was two. Retinal detachment was present in 65% of cases. The primary...
Li, Y-X [author]; COUCKE, Philippe [author]; Li, J-Y [author]; Gu, D-Z [author]; Liu, X-F [author]; Mirimanoff, R-O [author]; Huang, Y-R [author]
BACKGROUND. This study was conducted to determine whether the paranasal
extension of a primary non-Hodgkin’s lymphoma (NHL) of the nasal cavity has any
deleterious effect on patient outcome.
METHODS. One hundred and seventy-five patients with previously untreated
nasal NHL were reviewed. There were 2 with low grade, 107 with intermediate
grade, 17 with high grade, and 49 with unclassifiable lymphomas. In 48
cases the immunophenotype was available and 46 were T-cell lymphoma.
According to the Ann Arbor system, there were 133 patients with Stage IE, 28
with Stage IIE, 4 with Stage IIIE, and 10 with Stage IVE lymphomas. Stage IE
was subdivided into limited Stage IE (i.e., confined...
Maingon, Philippe [author]; COUCKE, Philippe [author]; Haie-Meder, Catherine [author]; Barillot, Isabelle [author]
Le traitement des cancers du col utérin repose sur la
radiothérapie et la chirurgie. Le pronostic des formes
évoluées a conduit à proposer différentes modalités
d’association dont seuls les traitements concomitants ont
montré un intérêt. Les associations de radiothérapie et d’un
radiosensibilisant n’ont pas apporté, de bénéfice, voire
abouti à des résultats inférieurs à ceux de l’irradiation
exclusive. L’Hydroxyurée et la Mitomycine C ont été
largement testées, seules ou en association, sans qu’elles
puissent être retenues comme efficaces. Des associations
modernes et des essais récents, il peut être établi que seul
le Cisplatine (et peut-être ses dérivés) peut être retenu dans
!es propositions thérapeutiques futures. 1998 Elsevier,
Coucke, Philippe [author]; Zouhair, A [author]; Ozsahin, M [author]; De Tribolet, N [author]; Mirimanoff, R-O [author]
Purpose: To investigate whether whole brain irradiation might be replaced by focalized irradiation after resection of a single brain metastasis in patients where extracranial tumor control is deemed to be obtained.
Patients and methods: Twelve patients were introduced in a phase I/II prospective study of conformal postoperative external irradiation after resection of a solitary brain metastasis. The radiation treatment consisted of 50.4 Gy (1.8 Gy per fraction, five fractions per week). The planning target volume consisted of the tumor bed and a 2 cm safety margin. All treatments were optimized with head immobilization, dedicated tomodensitometry and computer assisted three-dimensional treatment planning.
Ozsahin, M; Zouhair, A; Villa, S; Storme, G; Chauvet, B; Taussky, D; Gouder, D; Ries, G; Bontemps, P; COUCKE, Philippe; Mirimanoff, R-O
Decoster, L-A [author]; Chen, X [author]; Lejeune, F [author]; Mirimanoff, R-O [author]; Biolla, J [author]; COUCKE, Philippe [author]
Simultaneous determination of ribonucleoside and deoxyribonucleoside
triphosphates in cells by HPLC is an
analytical challenge since the concentration of dNTP
present in mammalian cells is several orders of magnitude
lower than the corresponding NTP. Hence, the
quantitation of dNTP in cells is generally performed
after selective oxidation or removal of the major NTP.
The procedures reported so far are lengthy and cumbersome
and do not enable the simultaneous determination
of NTP. We report the development of a simple, direct
HPLC method for the simultaneous determination of
dNTP and NTP in colon carcinoma WiDr cell extracts
using a stepwise gradient elution ion-pairingHPLCwith
uv detection at 260 nm and with a minimal chemical
manipulation of cells....
Wilson, G-D [author]; Paschoud, N [author]; Pavy, J-J [author]; Weber, K. [author]; Weber, P. [author]; Dubray, B [author]; COUCKE, Philippe [author]
Summary We compared the flow cytometric measurement and analysis of the potential doubling time ( Tpot) between three centres involved in
the National Cancer Institute (NCI) protocol T92-0045. The primary purpose was to understand and minimize the variation within the
measurement. A total of 102 specimens were selected at random from patients entered into the trial. Samples were prepared, stained, run
and analysed in each centre and a single set of data analysed by all three centres. Analysis of the disc data set revealed that the
measurement of labelling index (LI) was robust and reproducible. The estimation of duration of S-phase ( Ts) was...
LI, Y-X [author]; Sun, L-Q [author]; Paschoud, N [author]; Weber-Johnson, K [author]; COUCKE, Philippe [author]
(E)-28-deoxy-28-(fluoromethylene) cytidine (FMdC), a novel
inhibitor of ribonucleotide-diphosphate reductase, has been
shown to have anti-tumor activity against solid tumors and
sensitize tumor cells to ionizing radiation. Pentoxifylline
(PTX) can potentiate the cell killing induced by DNAdamaging
agents through abrogation of DNA-damagedependent
G2 checkpoint. We investigated the cytotoxic,
radiosensitizing and cell-cycle effects of FMdC and PTX in a
human colon-cancer cell line WiDr. PTX at 0.25–1.0 mM
enhanced the cytotoxicity of FMdC and lowered the IC50 of
FMdC from 79 6 0.1 to 31.2 6 2.1 nM, as determined by MTT
assay. Using clonogenic assay, pre-irradiation exposure of
exponentially growing WiDr cells to 30 nM FMdC for 48 hr or
post-irradiation to 0.5 to...
Zouhair, A [author]; Ozsahin, M [author]; Mouhsine, E [author]; Leyvraz, P-F [author]; Mirimanoff, R-O [author]; COUCKE, Philippe [author]
External radiotherapy or non-steroid antiinflammatory
drugs for prevention of
heterotopic ossification following total
Heterotopic ossification (HO) is defined as
the development of abnormal ossification in
soft tissues. HO is a common disease after
total hip replacement. Many therapeutic modalities
have been proposed to prevent HO.
The most commonly used modalities are nonsteroidal
anti-inflammatory drugs (NSAID) or
ionizing radiation administered just before or
immediately after total hip replacement. As far
as external radiation therapy is concerned,
there are several published randomized studies
aimed at investigating its efficacy and timing
related to surgery, and at comparing ionizing
irradiation to NSAID. In this article we review
the published data in order to define guidelines
which could be used in daily...
Koutaissoff, S [author]; Wellmann, D [author]; COUCKE, Philippe [author]; Ozsahin, M [author]; Pampallona, S [author]; Mirimanoff, R-O [author]
Purpose: To evaluate a hyperfractionated and accelerated radiotherapy (HART) protocol in patients with inoperable non-small cell lung carcinoma (NSCLC) who were ineligible for combination radiochemotherapy
Methods and Materials: From February 1989 through August 1994, 23 patients ineligible for available combined
modality protocols in our institution were enrolled and treated with HART, consisting of 63 Gy given in 42 fractions of 1.5 Gy each, twice daily, with a minimum time interval of 6 h between fractions, 5 days a week, over an elapsed time of 4.2 weeks, or 29 days. There was no planned interruption.
Results: The 1-, 2-, and 3-year survival rates were...
Crompton, Nigel; Miralbell, Raymond; Rutz, Hans-Peter; Ersoy, Fügen; Sanal, Özden; Wellmann, Danielle; Bieri, Sabine; Emery, Gillian; Shi, Yu-Quan; COUCKE, Philippe; OzsahinBlattmann; Ozsahin
Coucke, Philippe [author]; Decosterd, L-A [author]; Li, Y-X [author]; Cottin, E [author]; Chen, X [author]; Sun, L-Q [author]; Stern, S [author]; Paschoud, N [author]; Denekamp, J [author]
(E)-2*-Deoxy-(fluoromethylene)cytidine (FMdC) is known as an inhibitor
of ribonucleoside diphosphate reductase, a key enzyme in the de novo
pathway of DNA synthesis. FMdC was tested as a modifier of radiation
response in vitro on a human colon carcinoma cell line (WiDr), and the
observed radiosensitization was confirmed on two human cervix cancer
cell lines (C33-A and SiHa). Using the clonogenic assay, the effect ratio
(ER) at a clinically relevant dose level of 2 Gy was 2.10 (50 nM FMdC),
1.70 (30 nM FMdC), and 1.71 (40 nM FMdC) for the three cell lines WiDr,
C33-A, and SiHa, respectively. A more detailed analysis of the importance
of timing and concentration...
Lomax, T [author]; Goitein, G [author]; Debus, J [author]; Dykstra, C [author]; Tercier, P-A [author]; COUCKE, Philippe [author]; Mirimanoff, R-O [author]
Purpose: A comparative treatment planning study has been undertaken between standard photon delivery techniques,b intensity modulated photon methods and spot scanned protons in order to investigate the merits and limitations of each of these treatment approaches.
Methods: Plans for each modality were performed using CT scans and planning information for nine patients with varying indications and lesion sites and the results have been analysed using a variety of dose and volume based parameters.
Results: Over all cases, it is predicted that the use of protons could lead to a reduction of the total integral dose by a factor three compared to standard...
Coucke, Philippe [author]; Maingon, P [author]; Ciernik, I [author]; Do, H-P [author]
Background: The treatment outcome of advanced stage uterine cervical carcinoma remains unsatisfactory. In order to elaborate a novel
trial within The Radiotherapy Cooperative Group (RCG) of the European Organization for Research and Treatment of Cancer (EORTC), we
conducted a survey in 1997±1998 to determine the variability of pre-treatment assessment and treatment options. The variability of choosing
surgery, de®ned radiation therapy techniques and chemotherapy are investigated, as well as the center's choices of future treatment strategies.
Methods: Fifty two of 81 RCG centers from the RCG have participated in the survey. As one would expect, there is a large variation in the
techniques used for pretreatment...
Delaloye, J-F [author]; Pampallona, S [author]; COUCKE, Philippe [author]; Megalo, A [author]; Dr Grandi, P [author]
Objective : To analyse the effect of differentiation on disease-free survival (DFS) and overall survival (OS) in patients with stage I adenocarcinoma of the endometrium. Patients and methods : From 1979 to 1995, 350 patients with FIGO stage IA–IC with well (G1), moderately (G2) or poorly (G3) differentiated tumors were treated with surgery and high dose-rate brachytherapy with or without external radiation. Median age was 65 years (39–86 years). Results : The 5-year DFS was 8863% for the G1 tumors, 7764% for the G2 tumors,
and 6767% for the G3 tumors (P50.0049). With regard to the events contributing to DFS, the...
Coucke, Philippe; PHUOC DO, Hu; Pica, Alessia; Urban, Filip; Pache, Gilbert
Endovascular brachytherapy after percutaneous coronary intervention (PCI), is becoming a standard approach for the treatment and prevention of restenosis. A variety of technical approaches are currently available to deliver ionizing irradiation to the vascular target. Basically two kinds of radioactive isotopes are available that emit gamma radiation (photons) or beta radiation (electrons). The pitfalls and solutions for the optimization of dosimetry are discussed. As might be expected, the inhomogeneous dose distribution across the target volume
results in recurrence by underdosage or in complications because of overdosage. Moreover, uniformization of the target definition and reporting of the dose distribution in endovascular brachytherapy...
The one-and-a-half day Young Investigators Workshop was
held in the Holiday Inn, Bethesda, August 1–2, 2000. Using
the SCAROP mailing list and recommendations from department
chiefs, approximately 55 “early-career” physicianscientists
were invited to attend the workshop. Table 1
includes the participants.
The goals of the meeting were:
c To bring together radiation oncology physician-scientists
who were in the early part of their career to discuss
research ideas and opportunities as well as potential barriers
to progress for the field and for young-investigator
c To help develop camaraderie among and a critical-mass
of a new generation of physician-scientists with interests
ranging from technology development, to basic and translational
research, to outcomes research and analysis.