Briquet, Alexandra; Dolhet, Marie; Beguin, Yves; Gothot, André
38th Annual Scientific Meeting of the ISEH - society for Hematology and Stem Cells
Bouzourene, Hanifa; Bosman, Fred; Seelentag, Walter; Matter, Maurice; COUCKE, Philippe
BACKGROUND: Locally advanced rectal carcinoma has a poor prognosis. However,
since the introduction of preoperative radiotherapy, the outcome of patients with
rectal carcinoma has been reported to have improved. Nevertheless, to the authors’
knowledge few data are available regarding the histopathologic response to
radiotherapy as assessed on surgical specimens as a potential predictive factor for
METHODS: To estimate the effect of radiotherapy on rectal carcinoma, the authors
retrospectively reviewed the surgical specimens of 102 patients with T3-4, N0 or
N1 rectal carcinoma and 1 patient with T2 but N1 rectal carcinoma. All patients
were treated preoperatively with a...
Voelter; Zouhair; Vuilleumier; Matter; Bouzourene; Leyvra; Bauer; COUCKE, Philippe; Stupp
Patients with rectal cancer are at high risk of disease recurrence despite neoadjuvant radiochemotherapy with 5-Fluorouracil (5FU), a
regimen that is now widely applied. In order to develop a regimen with increased antitumour activity, we previously established the
recommended dose of neoadjuvant CPT-11 (three times weekly 90 mgm 2) concomitant to hyperfractionated accelerated
radiotherapy (HART) followed by surgery within 1 week. Thirty-three patients (20 men) with a locally advanced adenocarcinoma of
the rectum were enrolled in this prospective phase II trial (1 cT2, 29 cT3, 3 cT4 and 21 cNþ). Median age was 60 years (range 43–
75 years). All...
Nikkels, Arjen; Hansen, Isabelle; Collignon, Jacques; Fassotte, M F; Fillet, Georges; Pierard, Gérald
Neutrophilic eccrine hidradenitis (NEH) is a rare neutrophilic dermatosis occurring most frequently during induction chemotherapy for a variety of malignancies. We report a case of NEH in a 41-year-old woman with acute myeloblastic leukemia under daunorubicin, cytarabine and etoposide chemotherapy. She developed red, tender and painful nodules on a shoulder. The lesions resolved spontaneously. Histological examination is mandatory as the clinical presentation of this dermatosis is highly polymorphic. Leukemia cutis, sepsis, deep fungal infection and Sweet's syndrome must be excluded as these implicate different therapies.
Lechanteur, Chantal; Delvenne, Philippe; Princen, Frédéric; Lopez Y Cadenas, Miguel; Fillet, Georges; Gielen, Jacques; Merville, Marie-Paule; Bours, Vincent
BACKGROUND: Gene therapy is a novel approach for the treatment of cancers, and tumours disseminated in the peritoneal cavity are suitable for in situ delivery of a therapeutic gene. AIMS: The efficacy of a therapy combining a suicide gene (herpes simplex virus type I thymidine kinase (HSV-TK)) and cytokine genes was investigated in a model of peritoneal carcinomatosis induced by colon carcinoma cells in syngeneic rats. MATERIAL AND METHODS: Pre-established macroscopic tumours in BDIX rats were treated by intraperitoneal injections of retrovirus producing cells (FLYA13 TK, FLYA13 granulocyte macrophage-colony stimulating factor (GM-CSF), FLYA13 interleukin 12 (IL-12)) and ganciclovir (GCV). RESULTS:...
Coucke, Philippe; Crompton
Briquet, Alexandra; Beguin, Yves; Gothot, André
Lachatre, F; Gothot, André
D-dimers are fibrin degradation products which are released during local or systemic activation of coagulation. D-dimer testing is widely used for the work-up of patients suspected of deep vein thrombosis or pulmonary embolism. Interpretation of D-dimer levels must take into account the sensitivity and specificity of the assay. The specificity is usually low, and increased D-dimer levels are encountered in many non-thrombotic situations. The sensitivity varies among the different testing methods and is maximal with ELISA assays. Highly sensitive assays allow exclusion of venous thromboembolism when D-dimer levels are normal, without further imaging. However, this diagnostic strategy may only be...
Begg, A-C [author]; Hart, AAM [author]; Dische, S [author]; Saunders, M [author]; COUCKE, Philippe [author]; Paschoud [author]; Hoyer, M [author]; Overgaard, J [author]; Antognoni, P [author]; Richetti, A [author]; Bourhis, J [author]; Bartelink, H [author]; Horiot, J-C [author]; Corvo, R [author]; Giaretti, W [author]; Awwad, H [author]; Maciorowski, Z [author]; Dobrowsky, W [author]; Struikmans, H [author]; Wilson, GD [author]
Purpose: The aim of this study was to assess the potential of pre-treatment cell kinetic parameters to predict outcome in head and neck cancer patients treated by conventional radiotherapy.
Materials and methods: Data from 11 different centers were pooled. Inclusion criteria were such that the patients received radiotherapy alone, and that the radiotherapy was given in an overall time of at least 6 weeks with a dose of at least 60 Gy. All patients received a tracer dose of either iododeoxyuridine (IdUrd) or bromodeoxyuridine (BrdUrd) intravenously prior to treatment and a tumor biopsy was taken several hours later. The cell kinetic...
Copaceanu, M-L [author]; COUCKE, Philippe [author]; Cottin, E [author]; Mirimanoff, R-O [author]
The potential effect of AZT as a thymidine analogue on radiation response in vitro was investigated. Two human cell lines (WiDr and HeLa) were used. The effect of 10 μM AZT on exponentially growing cells was studied after different exposure times (24, 48 and 72 h). The surviving fraction (clonogenic assay) or metabolic activity (MTT assay) after irradiation of AZT-exposed cells, was compared to unexposed irradiated controls. Flow cytometry was used to assess the cell-cycle effect of pre-exposure of exponentially growing cells to AZT. AZT had a radioprotective effect for all experimental time points as far as WiDr was concerned....
Miralbell, R [author]; COUCKE, Philippe [author]; Behrouz, F [author]; Blazek, N [author]; Melliger, M [author]; Philipp, S [author]; Wickenhauser, R [author]; Schwabb, R [author]; Rosset, A [author]; Hagen, G [author]; Douglas, P [author]; Bieri, S [author]; Allal, A [author]; Majno, S [author]; Bardina, A [author]
A prospective trial was performed to better assess the risk of nausea and vomiting and the rescue value of
tropisetron (TRO), a 5HT3 receptor antagonist, in 88 patients undergoing fractionated radiotherapy to the
abdomen or to large supradiaphragmatic fields and failing a first anti-emetic trial with metoclopramide (MET).
Nausea was graded 0 (absent), 1 (mild), 2 (moderate) and 3 (severe). Nausea requiring anti-emetics (L grade 2)
was present in 64% of the patients. MET was able to control nausea (I grade 1) in 26 of 58 patients (45%) who
developed 1 grade 2 nausea during radiation treatment (2 patients vomiting without nausea included). 34 patients
COUCKE, Philippe [author]
Buchegger, F [author]; Rojas, A [author]; Delaloye, A [author]; Vogel, C-A [author]; Mirimanoff, R-O [author]; COUCKE, Philippe [author]; Denekamp, J [author]; Mach, J-P [author]
The effect of combined radioimmunotherapy (RIT) and fractionated
external beam radiotherapy (RT) was assessed in two human colon cancer
xenografts, Col 12 and LS174T in nude mice. These tumors were selected
for being resistant to RIT alone, as is usually the case in the clinical
situation. Tumor-bearing mice were treated with a combination of five
X-ray fractions over 5 days followed by RIT with two doses of 1.5 mCi
131I-labeled anticarcinoembryonic antigen monoclonal antibody F(ab')2.
In Col 12 and LS174T, RIT alone achieved a regrowth delay similar to
that of fractionated RT with total doses of 28 and 26 Gy, respectively. In
both tumor types, an additive therapeutic effect,...
COUCKE, Philippe; Sartirelli, Britta; Cuttat, Jean-François; Jeanneret, Wendy; Gillet, Michel; Mirimanoff, René-Olivier
Delaloye, J-F [author]; COUCKE, Philippe [author]; Pampallona, S [author]; De Grandi, P [author]
Delaloye, J-F [author]; Pampallona, S [author]; COUCKE, Philippe [author]; De Grandi, P [author]
Objective: To verify the influence of age on the prognosis of cervix carcinoma. Study design: Five hundred and sixty eight patients
treated for a FIGO stage IB-IVA with radical irradiation in the Centre Hospitalier Universitaire Vaudois of Lausanne were subdivided
according to the following age categories: _<45, 46-60, 61-69 and > 70 years. Taking the 46-60 years age group as the
reference, the hazard ratios (HR) of death and corresponding 95% confidence intervals (95% CI) were estimated by means of a
Cox multivariate analysis. Results: The 5-year survival rates were, respectively, 57%, 67%, 60% and 45%. For the youngest women
the risk of death was...
Li; COUCKE, Philippe; Qian; Huang, Yi-Rong; Gu, Da-Zhong; Mirimanoff, René-Olivier; Yu, Zi-Hao
Purpose: To analyze the clinical characteristics, prognosis, and treatment outcome of pelvic cryptorchid seminoma
(PCS), and to determine whether whole abdominal-pelvic irradiation for Stage I disease is necessary.
Methods and Materials: From 1958 to 1991,60 patients with PCS were treated at the Cancer Hospital of Chinese
Academy of Medical Sciences, Beijing. They presented with a lower abdominal mass and showed a predominance
for the right side. A high proportion of patients with PCS [ 26 of 60 (43% )] had metastatic disease, compared to
20% of those with scrotal seminoma, and there was a tendency toward a higher frequency of pelvic nodal metastases.
There were 34...
Li, Y-X [author]; COUCKE, Philippe [author]; Qian, T-L [author]; Huang, Y-R [author]; Gu, D-Z [author]; Mirimanoff, R-O [author]; Yu, Z-H [author]
Purpose: The purpose of this study was to analyze prognosis and treatment results for seminoma arising in
corrected and uncorrected inguinal cryptorchidism (SCIC and SUIC).
Methods and Materials: We reviewed 66 patients with inguinal seminomas between June 1958 and December
1991 at the Cancer Hospital and Institute of Chinese Academy of Medical Sciences. Of these patients, 23 had
prior orchiopexy and 43 presented with an inguinal form of cryptorchidism. At presentation, 17 of 66 (26%)
patients had nodal metastases. This nodal involvement was 30% (7 of 23) for SCIC and 23% (10 of 43) for SUIC,
respectively. These numbers are comparable with those in a series...
Coucke, Philippe; Li, Ye-Xiong; Copaceanu, Marie-Laure; Paschoud, Nicolas; Cottin, Eliane; Ozsahin, Mahmut; Mirimanoff
The potential of zalcitabine (ddC) to act as an ionizing radiation response modifier was tested on exponentially growing human cancer
cells in vitro. Two human cell lines, WiDr (colon) and MCF-7 (breast) were exposed to ddC at 10 p M concentration for various lengths
of tide (18, 24, 48 and 72 h). On the WiDr cell line the dual effect of concentration and duration of exposure prior to irradiation was
investigated. Experimental endpoints were clonogenicity and viability, as measured by colony formation assay (CFA) and MTT assay
respectively. The impact on cell-cycle distribution prior to irradiation was assessed by flow cytometry using a double...