PubMed Central (PMC3 - NLM DTD)
Mostrando recursos 81 - 100 de 599
Generation of single-copy transgenic mouse embryos directly from ES cells by tetraploid embryo complementation
- Misra, Ravi P; Bronson, Sarah K; Xiao, Qi; Garrison, Wendy; Li, Jixuan; Zhao, Roong; Duncan, Stephen A
A combined in vitro / in vivo selection for polymerases with novel promoter specificities
- Chelliserrykattil, Jijumon; Cai, George; Ellington, Andrew D
Optimisation of transgene action at the post-transcriptional level: high quality parthenocarpic fruits in industrial tomatoes
- Pandolfini, Tiziana; Rotino, Giuseppe L; Camerini, Serena; Defez, Roberto; Spena, Angelo
A fluorescent cassette-based strategy for engineering multiple domain fusion proteins
- Truong, Kevin; Khorchid, Ahmad; Ikura, Mitsuhiko
Characterization of mutations and loss of heterozygosity of p53 and K-ras2 in pancreatic cancer cell lines by immobilized polymerase chain reaction
- Butz, James; Wickstrom, Eric; Edwards, Jeremy
An automated method for high-throughput protein purification applied to a comparison of His-tag and GST-tag affinity chromatography
- Scheich, Christoph; Sievert, Volker; Büssow, Konrad
Hydrodynamic guiding for addressing subsets of immobilized cells and molecules in microfluidic systems
- Brevig, Thomas; Krühne, Ulrich; Kahn, Rachel A; Ahl, Thomas; Beyer, Michael; Pedersen, Lars H
A new reverse transcription-polymerase chain reaction method for accurate quantification
- Shiao, Yih-Horng
Recombining overlapping BACs into a single larger BAC
- Kotzamanis, George; Huxley, Clare
Gene expression analysis on small numbers of invasive cells collected by chemotaxis from primary mammary tumors of the mouse
- Wang, Weigang; Wyckoff, Jeffrey B; Wang, Yarong; Bottinger, Erwin P; Segall, Jeffrey E; Condeelis, John S
Expression of self-complementary hairpin RNA under the control of the rolC promoter confers systemic disease resistance to plum pox virus without preventing local infection
- Pandolfini, Tiziana; Molesini, Barbara; Avesani, Linda; Spena, Angelo; Polverari, Annalisa
Evaluation of five different cDNA labeling methods for microarrays using spike controls
- Badiee, Azadeh; Eiken, Hans Geir; Steen, Vidar M; Løvlie, Roger
Quantitative Real Time Polymerase Chain Reaction for measurement of human Interleukin â?? 5 receptor alpha spliced isoforms mRNA
- Pérez, Claudina; Vandesompele, Jo; Vandenbroucke, Ina; Holtappels, Gabriele; Speleman, Frank; Gevaert, Philippe; Van cauwenberge, Paul; Bachert, Claus
Real-time PCR based on SYBR-Green I fluorescence: An alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications and micro gene deletions
- Ponchel, Frederique; Toomes, Carmel; Bransfield, Kieran; Leong, Fong T; Douglas, Susan H; Field, Sarah L; Bell, Sandra M; Combaret, Valerie; Puisieux, Alain; Mighell, Alan J; Robinson, Philip A; Inglehearn, Chris F; Isaacs, John D; Markham, Alex F
Chemically defined medium and Caenorhabditis elegans
- Szewczyk, Nathaniel J; Kozak, Elena; Conley, Catharine A
Coupling of importin beta binding peptide on plasmid DNA: transfection efficiency is increased by modification of lipoplex's physico-chemical properties
- Carrière, Marie; Escriou, Virginie; Savarin, Aline; Scherman, Daniel
Performance of high-throughput DNA quantification methods
- Haque, Kashif A; Pfeiffer, Ruth M; Beerman, Michael B; Struewing, Jeff P; Chanock, Stephen J; Bergen, Andrew W
Expressing functional siRNAs in mammalian cells using convergent transcription
- Tran, Nham; Cairns, Murray J; Dawes, Ian W; Arndt, Greg M
In vivo mucosal delivery of bioactive human interleukin 1 receptor antagonist produced by Streptococcus gordonii
- Ricci, Susanna; Macchia, Giovanni; Ruggiero, Paolo; Maggi, Tiziana; Bossù, Paola; Xu, Li; Medaglini, Donata; Tagliabue, Aldo; Hammarström, Lennart; Pozzi, Gianni; Boraschi, Diana
Expression of a crown gall biological control phenotype in an avirulent strain of Agrobacterium vitis by addition of the trifolitoxin production and resistance genes
- Herlache, Thomas C; Triplett, Eric W
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