PubMed Central (PMC3 - NLM DTD)
Archive of life sciences journal literature at the U.S. National Institutes of Health (NIH), developed and managed by NIH's National Center for Biotechnology Information (NCBI) in the National Library of Medicine (NLM).
Journal of Bacteriology
Mostrando recursos 1 - 20 de 93.978
Hyperosmotic Stress Response of Campylobacter jejuni - Cameron, Andrew; Frirdich, Emilisa; Huynh, Steven; Parker, Craig T.; Gaynor, Erin C.
The diarrheal pathogen Campylobacter jejuni and other gastrointestinal bacteria encounter changes in osmolarity in the environment, through exposure to food processing, and upon entering host organisms, where osmotic adaptation can be associated with virulence. In this study, growth profiles, transcriptomics, and phenotypic, mutant, and single-cell analyses were used to explore the effects of hyperosmotic stress exposure on C. jejuni. Increased growth inhibition correlated with increased osmotic concentration, with both ionic and nonionic stressors inhibiting growth at 0.620 total osmol liter−1. C. jejuni adaptation to a range of osmotic stressors and concentrations was accompanied by severe filamentation in subpopulations, with microscopy...
Analysis of the Regulated Transcriptome of Neisseria meningitidis in Human Blood Using a Tiling Array - Del Tordello, Elena; Bottini, Silvia; Muzzi, Alessandro; Serruto, Davide
Neisseria meningitidis is the major cause of septicemia and meningococcal meningitis. During the course of infection, the bacterium must adapt to different host environments as a crucial factor for survival and dissemination; in particular, one of the crucial factors in N. meningitidis pathogenesis is the ability to grow and survive in human blood. We recently showed that N. meningitidis alters the expression of 30% of the open reading frames (ORFs) of the genome during incubation in human whole blood and suggested the presence of fine regulation at the gene expression level in order to control this step of pathogenesis. In...
Draft Genome Sequence of Alicyclobacillus hesperidum Strain URH17-3-68 - Wang, Ping; Li, Li; Chen, Xiaoyun; Jiang, Nan; Liu, Guiyou; Chen, Lawrence; Xu, Jianyong; Song, Hui; Chen, Zugen; Ma, Yanhe
Alicyclobacillus hesperidum is a thermoacidophilic bacterium. We isolated strain URH17-3-68 from hot spring sludge in Tengchong, Yunnan province, China. Its extracellular products include heat- and acid-stable enzymes which are important for industrial applications. Here we report the draft genome of this strain.
Draft Genome Sequence of a Nonhemolytic Fish-Pathogenic Streptococcus agalactiae Strain - Delannoy, Christian M. J.; Zadoks, Ruth N.; Lainson, Frederick A.; Ferguson, Hugh W.; Crumlish, Margaret; Turnbull, James F.; Fontaine, Michael C.
Streptococcus agalactiae is a significant Gram-positive bacterial pathogen of terrestrial and aquatic animals. A subpopulation of nonhemolytic strains which appear to be pathogenic only for poikilotherms exists. We report here the first draft genome sequence of a nonhemolytic S. agalactiae isolate recovered from a diseased fish.
Genome Sequence of Pseudomonas mendocina DLHK, Isolated from a Biotrickling Reactor - Wong, Chi Fat; Niu, Hejingying; Jiang, Jingwei; Li, Jun; Chan, Charis May Ngor; Leung, Dennis Yiu Cheong; Leung, Frederick Chi Ching
Pseudomonas mendocina DLHK is an aerobic bacterium isolated from a biotrickling reactor which can remove nitric oxide, a common air pollutant from combustion exhaust gas. Here, we present the draft genome of Pseudomonas mendocina DLHK.
SurA Is Involved in the Targeting to the Outer Membrane of a Tat Signal Sequence-Anchored Protein - Rondelet, Arnaud; Condemine, Guy
The twin arginine translocation (Tat) pathway exports folded proteins from the cytoplasm to the periplasm of bacteria. The targeting of the exported proteins to the Tat pathway relies on a specific amino-terminal signal sequence, which is cleaved after exportation. In the phytopathogen Dickeya dadantii, the pectin lyase homologue PnlH is exported by the Tat pathway without cleavage of its signal sequence, which anchors PnlH into the outer membrane. In proteobacteria, the vast majority of outer membrane proteins consists of β-barrel proteins and lipoproteins. Thus, PnlH represents a new kind of outer membrane protein. In Escherichia coli, periplasmic chaperones SurA, Skp,...
Streptococcus pneumoniae Uses Glutathione To Defend against Oxidative Stress and Metal Ion Toxicity - Potter, Adam J.; Trappetti, Claudia; Paton, James C.
The thiol-containing tripeptide glutathione is an important cellular constituent of many eukaryotic and prokaryotic cells. In addition to its disulfide reductase activity, glutathione is known to protect cells from many forms of physiological stress. This report represents the first investigation into the role of glutathione in the Gram-positive pathogen Streptococcus pneumoniae. We demonstrate that pneumococci import extracellular glutathione using the ABC transporter substrate binding protein GshT. Mutation of gshT and the gene encoding glutathione reductase (gor) increases pneumococcal sensitivity to the superoxide generating compound paraquat, illustrating the importance of glutathione utilization in pneumococcal oxidative stress resistance. In addition, the gshT...
Draft Genome Sequence of Fusobacterium nucleatum ChDC F128, Isolated from a Periodontitis Lesion - Park, Soon-Nang; Kong, Si-Won; Kim, Hwa-Sook; Park, Mo-Se; Lee, Jee-Won; Cho, Eugene; Lim, Yun Kyong; Choi, Mi-Hwa; Chang, Young-Hyo; Shin, Jeong Hwan; Park, Hong-Seog; Choi, Sang-Haeng; Kook, Joong-Ki
Fusobacterium nucleatum is classified into five subspecies. F. nucleatum ChDC F128 was isolated from a periodontitis lesion and proposed as a new subspecies based on the comparison of the nucleotide sequences of the RNA polymerase beta subunit and zinc protease genes. Here, we report the draft genome sequence of the strain.
Differential Control of the Rate of 5′-End-Dependent mRNA Degradation in Escherichia coli - Luciano, Daniel J.; Hui, Monica P.; Deana, Atilio; Foley, Patricia L.; Belasco, Kevin J.; Belasco, Joel G.
Many Escherichia coli mRNAs are degraded by a 5′-end-dependent mechanism in which RppH-catalyzed conversion of the 5′-terminal triphosphate to a monophosphate triggers rapid endonucleolytic cleavage by RNase E. However, little is understood about what governs the decay rates of these transcripts. We investigated the decay of three such messages—rpsT P1, yfcZ, and ydfG—to characterize the rate-determining step in their degradation. The steady-state ratio of monophosphorylated to triphosphorylated rpsT P1 and yfcZ mRNA indicates that their decay rate is limited by cleavage of the monophosphorylated intermediate, making RNase E critical for their rapid turnover. Conversely, the decay rate of ydfG is...
Draft Genome Sequence of Virgibacillus halodenitrificans 1806 - Lee, Sang-Jae; Lee, Yong-Jik; Jeong, Haeyoung; Lee, Sang Jun; Lee, Han-Seung; Pan, Jae-Gu; Kim, Byoung-Chan; Lee, Dong-Woo
Virgibacillus halodenitrificans 1806 is an endospore-forming halophilic bacterium isolated from salterns in Korea. Here, we report the draft genome sequence of V. halodenitrificans 1806, which may reveal the molecular basis of osmoadaptation and insights into carbon and anaerobic metabolism in moderate halophiles.
Characterization of a Ferrous Iron-Responsive Two-Component System in Nontypeable Haemophilus influenzae - Steele, Kendra H.; O'Connor, Lauren H.; Burpo, Nicole; Kohler, Katharina; Johnston, Jason W.
Nontypeable Haemophilus influenzae (NTHI), an opportunistic pathogen that is commonly found in the human upper respiratory tract, has only four identified two-component signal transduction systems. One of these, an ortholog to the QseBC (quorum-sensing Escherichia coli) system, was characterized. This system, designated firRS, was found to be transcribed in an operon with a gene encoding a small, predicted periplasmic protein with an unknown function, ygiW. The ygiW-firRS operon exhibited a unique feature with an attenuator present between ygiW and firR that caused the ygiW transcript level to be 6-fold higher than the ygiW-firRS transcript level. FirRS induced expression of ygiW...
Whole-Genome Sequence of Cupriavidus sp. Strain BIS7, a Heavy-Metal-Resistant Bacterium - Hong, Kar Wai; Thinagaran, Dinaiz a/l; Gan, Han Ming; Yin, Wai-Fong; Chan, Kok-Gan
Cupriavidus sp. strain BIS7 is a Malaysian tropical soil bacterium that exhibits broad heavy-metal resistance [Co(II), Zn(II), Ni(II), Se(IV), Cu(II), chromate, Co(III), Fe(II), and Fe(III)]. It is particularly resistant to Fe(II), Fe(III), and Zn(II). Here we present the assembly and annotation of its genome.
Genome Sequence of Nitratireductor aquibiodomus Strain RA22 - Singh, Ajit; Jangir, Pramod Kumar; Kumari, Chanchal; Sharma, Rakesh
The genus Nitratireductor represents nitrate-reducing bacteria from the family Phyllobacteriaceae. Here we report the draft genome sequence of Nitratireductor aquibiodomus strain RA22, which contains 4,592,790 bp, with a G+C content of 61.30%, and has 4,241 protein coding genes.
Analysis of ThiC Variants in the Context of the Metabolic Network of Salmonella enterica - Palmer, Lauren D.; Dougherty, Michael J.; Downs, Diana M.
In bacteria, the 4-amino-hydroxymethyl-2-methylpyrimidine (HMP) moiety of thiamine is synthesized from 5-aminoimidazole ribotide (AIR), a branch point metabolite of purine and thiamine biosynthesis. ThiC is a member of the radical S-adenosylmethionine (AdoMet) superfamily and catalyzes the complex chemical rearrangement of AIR to HMP-P. As reconstituted in vitro, the ThiC reaction requires AdoMet, AIR, and reductant. This study analyzed variants of ThiC in vivo and in vitro to probe the metabolic network surrounding AIR in Salmonella enterica. Several variants of ThiC that required metabolic perturbations to function in vivo were biochemically characterized in vitro. Results presented herein indicate that the subtleties...