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PubMed Central (PMC3 - NLM DTD) (2,805,745 recursos)
Archive of life sciences journal literature at the U.S. National Institutes of Health (NIH), developed and managed by NIH's National Center for Biotechnology Information (NCBI) in the National Library of Medicine (NLM).

Microbial Cell Factories

Mostrando recursos 1 - 20 de 951

1. Identification of a heterologous cellulase and its N-terminus that can guide recombinant proteins out of Escherichia coli - Gao, Dongfang; Wang, Shengjun; Li, Haoran; Yu, Huili; Qi, Qingsheng

2. Identification of a cluster-situated activator of oxytetracycline biosynthesis and manipulation of its expression for improved oxytetracycline production in Streptomyces rimosus - Yin, Shouliang; Wang, Weishan; Wang, Xuefeng; Zhu, Yaxin; Jia, Xiaole; Li, Shanshan; Yuan, Fang; Zhang, Yuxiu; Yang, Keqian

3. A novel locus for mycelial aggregation forms a gateway to improved Streptomyces cell factories - van Dissel, Dino; Claessen, Dennis; Roth, Martin; van Wezel, Gilles P

4. Fungal cytochrome P450 monooxygenases of Fusarium oxysporum for the synthesis of ω-hydroxy fatty acids in engineered Saccharomyces cerevisiae - Durairaj, Pradeepraj; Malla, Sailesh; Nadarajan, Saravanan Prabhu; Lee, Pyung-Gang; Jung, Eunok; Park, Hyun Ho; Kim, Byung-Gee; Yun, Hyungdon

5. Dissecting microbial community structure and methane-producing pathways of a full-scale anaerobic reactor digesting activated sludge from wastewater treatment by metagenomic sequencing - Guo, Jianhua; Peng, Yongzhen; Ni, Bing-Jie; Han, Xiaoyu; Fan, Lu; Yuan, Zhiguo

6. Protein recovery from inclusion bodies of Escherichia coli using mild solubilization process - Singh, Anupam; Upadhyay, Vaibhav; Upadhyay, Arun Kumar; Singh, Surinder Mohan; Panda, Amulya Kumar
Formation of inclusion bodies in bacterial hosts poses a major challenge for large scale recovery of bioactive proteins. The process of obtaining bioactive protein from inclusion bodies is labor intensive and the yields of recombinant protein are often low. Here we review the developments in the field that are targeted at improving the yield, as well as quality of the recombinant protein by optimizing the individual steps of the process, especially solubilization of the inclusion bodies and refolding of the solubilized protein. Mild solubilization methods have been discussed which are based on the understanding of the fact that protein molecules...

7. Metabolic network analysis and experimental study of lipid production in Rhodosporidium toruloides grown on single and mixed substrates - Bommareddy, Rajesh Reddy; Sabra, Wael; Maheshwari, Garima; Zeng, An-Ping

8. An analysis of the changes in soluble hydrogenase and global gene expression in Cupriavidus necator (Ralstonia eutropha) H16 grown in heterotrophic diauxic batch culture - Jugder, Bat-Erdene; Chen, Zhiliang; Ping, Darren Tan Tek; Lebhar, Helene; Welch, Jeffrey; Marquis, Christopher P

9. Dynamic control of ERG9 expression for improved amorpha-4,11-diene production in Saccharomyces cerevisiae - Yuan, Jifeng; Ching, Chi-Bun

10. An ancient Chinese wisdom for metabolic engineering: Yin-Yang - Wu, Stephen G; He, Lian; Wang, Qingzhao; Tang, Yinjie J
In ancient Chinese philosophy, Yin-Yang describes two contrary forces that are interconnected and interdependent. This concept also holds true in microbial cell factories, where Yin represents energy metabolism in the form of ATP, and Yang represents carbon metabolism. Current biotechnology can effectively edit the microbial genome or introduce novel enzymes to redirect carbon fluxes. On the other hand, microbial metabolism loses significant free energy as heat when converting sugar into ATP; while maintenance energy expenditures further aggravate ATP shortage. The limitation of cell “powerhouse” prevents hosts from achieving high carbon yields and rates. Via an Escherichia coli flux balance analysis...

11. A quorum sensing-based in vivo expression system and its application in multivalent bacterial vaccine - Chu, Teng; Ni, Chunshan; Zhang, Lingzhi; Wang, Qiyao; Xiao, Jingfan; Zhang, Yuanxing; Liu, Qin

12. Serine protease inhibitors protect better than IL-10 and TGF-β anti-inflammatory cytokines against mouse colitis when delivered by recombinant lactococci - Bermúdez-Humarán, Luis G; Motta, Jean-Paul; Aubry, Camille; Kharrat, Pascale; Rous-Martin, Laurence; Sallenave, Jean-Michel; Deraison, Céline; Vergnolle, Nathalie; Langella, Philippe

13. Microbial host selection affects intracellular localization and activity of alcohol-O-acetyltransferase - Zhu, Jie; Lin, Jyun-Liang; Palomec, Leidy; Wheeldon, Ian

14. Bioprocess automation on a Mini Pilot Plant enables fast quantitative microbial phenotyping - Unthan, Simon; Radek, Andreas; Wiechert, Wolfgang; Oldiges, Marco; Noack, Stephan

15. Annual acknowledgement of manuscript reviewers - Villaverde, Antonio
The Editors of Microbial Cell Factories would like to thank all our reviewers who have contributed to the journal in Volume 13 (2014).

16. Heterologous expression of a recombinant lactobacillal β-galactosidase in Lactobacillus plantarum: effect of different parameters on the sakacin P-based expression system - Nguyen, Tien-Thanh; Nguyen, Hoang-Minh; Geiger, Barbara; Mathiesen, Geir; Eijsink, Vincent GH; Peterbauer, Clemens K; Haltrich, Dietmar; Nguyen, Thu-Ha

17. Metabolic engineering of Escherichia coli for enhanced arginine biosynthesis - Ginesy, Mireille; Belotserkovsky, Jaroslav; Enman, Josefine; Isaksson, Leif; Rova, Ulrika

18. Effect of plasmid replication deregulation via inc mutations on E. coli proteome & simple flux model analysis - Meade, Jonathan; Bartlow, Patrick; Trivedi, Ram Narayan; Akhtar, Parvez; Ataai, Mohammad M; Khan, Saleem A; Domach, Michael M
When the replication of a plasmid based on sucrose selection is deregulated via the inc1 and inc2 mutations, high copy numbers (7,000 or greater) are attained while the growth rate on minimal medium is negligibly affected. Adaptions were assumed to be required in order to sustain the growth rate. Proteomics indicated that indeed a number of adaptations occurred that included increased expression of ribosomal proteins and 2-oxoglutarate dehydrogenase. The operating space prescribed by a basic flux model that maintained phenotypic traits (e.g. growth, byproducts, etc.) within typical bounds of resolution was consistent with the flux implications of the proteomic changes.

19. Process development for the production of 15β-hydroxycyproterone acetate using Bacillus megaterium expressing CYP106A2 as whole-cell biocatalyst - Kiss, Flora M; Lundemo, Marie T; Zapp, Josef; Woodley, John M; Bernhardt, Rita

20. Genome reduction boosts heterologous gene expression in Pseudomonas putida - Lieder, Sarah; Nikel, Pablo I; de Lorenzo, Víctor; Takors, Ralf

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