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PubMed Central (PMC3 - NLM DTD) (2,707,874 recursos)
Archive of life sciences journal literature at the U.S. National Institutes of Health (NIH), developed and managed by NIH's National Center for Biotechnology Information (NCBI) in the National Library of Medicine (NLM).

Microbial Cell Factories

Mostrando recursos 1 - 20 de 861

1. Cell factories for insulin production - Baeshen, Nabih A; Baeshen, Mohammed N; Sheikh, Abdullah; Bora, Roop S; Ahmed, Mohamed Morsi M; Ramadan, Hassan A I; Saini, Kulvinder Singh; Redwan, Elrashdy M
The rapid increase in the number of diabetic patients globally and exploration of alternate insulin delivery methods such as inhalation or oral route that rely on higher doses, is bound to escalate the demand for recombinant insulin in near future. Current manufacturing technologies would be unable to meet the growing demand of affordable insulin due to limitation in production capacity and high production cost. Manufacturing of therapeutic recombinant proteins require an appropriate host organism with efficient machinery for posttranslational modifications and protein refolding. Recombinant human insulin has been produced predominantly using E. coli and Saccharomyces cerevisiae for therapeutic use in...

2. Inorganic phosphate is a trigger factor for Microbispora sp. ATCC-PTA-5024 growth and NAI-107 production - Giardina, Anna; Alduina, Rosa; Gallo, Giuseppe; Monciardini, Paolo; Sosio, Margherita; Puglia, Anna Maria

3. Using ionic liquids in whole-cell biocatalysis for the nucleoside acylation - Yang, Meiyan; Wu, Hui; Lian, Yan; Li, Xiaofeng; Ren, Yao; Lai, Furao; Zhao, Guanglei

4. Development of a GIN11/FRT-based multiple-gene integration technique affording inhibitor-tolerant, hemicellulolytic, xylose-utilizing abilities to industrial Saccharomyces cerevisiae strains for ethanol production from undetoxified lignocellulosic hemicelluloses - Hasunuma, Tomohisa; Hori, Yoshimi; Sakamoto, Takatoshi; Ochiai, Misa; Hatanaka, Haruyo; Kondo, Akihiko

5. Catechol biosynthesis from glucose in Escherichia coli anthranilate-overproducer strains by heterologous expression of anthranilate 1,2-dioxygenase from Pseudomonas aeruginosa PAO1 - Balderas-Hernández, Víctor E; Treviño-Quintanilla, Luis G; Hernández-Chávez, Georgina; Martinez, Alfredo; Bolívar, Francisco; Gosset, Guillermo

6. Calcium-containing phosphopeptides pave the secretory pathway for efficient protein traffic and secretion in fungi - Martín, Juan F
Casein phosphopeptides (CPPs) containing chelated calcium drastically increase the secretion of extracellular homologous and heterologous proteins in filamentous fungi. Casein phosphopeptides released by digestion of alpha − and beta-casein are rich in phosphoserine residues (SerP). They stimulate enzyme secretion in the gastrointestinal tract and enhance the immune response in mammals, and are used as food supplements. It is well known that casein phosphopeptides transport Ca2+ across the membranes and play an important role in Ca2+ homeostasis in the cells.

7. Fermentation of oxidized hexose derivatives by Clostridium acetobutylicum - Servinsky, Matthew D; Liu, Sanchao; Gerlach, Elliot S; Germane, Katherine L; Sund, Christian J

8. Influence of pH control in the formation of inclusion bodies during production of recombinant sphingomyelinase-D in Escherichia coli - Castellanos-Mendoza, Andrea; Castro-Acosta, Ricardo M; Olvera, Alejandro; Zavala, Guadalupe; Mendoza-Vera, Miguel; García-Hernández, Enrique; Alagón, Alejandro; Trujillo-Roldán, Mauricio A; Valdez-Cruz, Norma A

9. Secretion of a foreign protein from budding yeasts is enhanced by cotranslational translocation and by suppression of vacuolar targeting - Fitzgerald, Ivy; Glick, Benjamin S

10. Deletion of the gene encoding the reductase component of 3-ketosteroid 9α-hydroxylase in Rhodococcus equi USA-18 disrupts sterol catabolism, leading to the accumulation of 3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid and 1,4-androstadiene-3,17-dione - Yeh, Chin-Hsing; Kuo, Yung-Shun; Chang, Che-Ming; Liu, Wen-Hsiung; Sheu, Meei-Ling; Meng, Menghsiao
The gene encoding the putative reductase component (KshB) of 3-ketosteroid 9α-hydroxylase was cloned from Rhodococcus equi USA-18, a cholesterol oxidase-producing strain formerly named Arthrobacter simplex USA-18, by PCR according to consensus amino acid motifs of several bacterial KshB subunits. Deletion of the gene in R. equi USA-18 by a PCR-targeted gene disruption method resulted in a mutant strain that could accumulate up to 0.58 mg/ml 1,4-androstadiene-3,17-dione (ADD) in the culture medium when 0.2% cholesterol was used as the carbon source, indicating the involvement of the deleted enzyme in 9α-hydroxylation of steroids. In addition, this mutant also accumulated 3-oxo-23,24-bisnorchola-1,4-dien-22-oic acid (Δ1,4-BNC). Because...

11. Secreted production of assembled Norovirus virus-like particles from Pichia pastoris - Tomé-Amat, Jaime; Fleischer, Lauren; Parker, Stephanie A; Bardliving, Cameron L; Batt, Carl A

12. Engineering Escherichia coli to overproduce aromatic amino acids and derived compounds - Rodriguez, Alberto; Martínez, Juan A; Flores, Noemí; Escalante, Adelfo; Gosset, Guillermo; Bolivar, Francisco
The production of aromatic amino acids using fermentation processes with recombinant microorganisms can be an advantageous approach to reach their global demands. In addition, a large array of compounds with alimentary and pharmaceutical applications can potentially be synthesized from intermediates of this metabolic pathway. However, contrary to other amino acids and primary metabolites, the artificial channelling of building blocks from central metabolism towards the aromatic amino acid pathway is complicated to achieve in an efficient manner. The length and complex regulation of this pathway have progressively called for the employment of more integral approaches, promoting the merge of complementary tools...

13. Bacterial cytoplasm as an effective cell compartment for producing functional VHH-based affinity reagents and Camelidae IgG-like recombinant antibodies - Djender, Selma; Schneider, Aurelie; Beugnet, Anne; Crepin, Ronan; Desrumeaux, Klervi Even; Romani, Chiara; Moutel, Sandrine; Perez, Franck; de Marco, Ario

14. Suitable extracellular oxidoreduction potential inhibit rex regulation and effect central carbon and energy metabolism in Saccharopolyspora spinosa - Zhang, Xiangmei; Xue, Chaoyou; Zhao, Fanglong; Li, Dashuai; Yin, Jing; Zhang, Chuanbo; Caiyin, Qinggele; Lu, Wenyu

15. High-quality production of human α-2,6-sialyltransferase in Pichia pastoris requires control over N-terminal truncations by host-inherent protease activities - Ribitsch, Doris; Zitzenbacher, Sabine; Augustin, Peter; Schmölzer, Katharina; Czabany, Tibor; Luley-Goedl, Christiane; Thomann, Marco; Jung, Christine; Sobek, Harald; Müller, Rainer; Nidetzky, Bernd; Schwab, Helmut

16. An Air-well sparging minifermenter system for high-throughput protein production - Deantonio, Cecilia; Sedini, Valentina; Cesaro, Patrizia; Quasso, Fabio; Cotella, Diego; Persichetti, Francesca; Santoro, Claudio; Sblattero, Daniele

17. MEP pathway-mediated isopentenol production in metabolically engineered Escherichia coli - Liu, Huaiwei; Wang, Yang; Tang, Qiang; Kong, Wentao; Chung, Wook-Jin; Lu, Ting

18. Pichia pastoris Aft1 - a novel transcription factor, enhancing recombinant protein secretion - Ruth, Claudia; Buchetics, Markus; Vidimce, Viktorija; Kotz, Daniela; Naschberger, Stefan; Mattanovich, Diethard; Pichler, Harald; Gasser, Brigitte

19. Functional recombinant protein is present in the pre-induction phases of Pichia pastoris cultures when grown in bioreactors, but not shake-flasks - Bawa, Zharain; Routledge, Sarah J; Jamshad, Mohammed; Clare, Michelle; Sarkar, Debasmita; Dickerson, Ian; Ganzlin, Markus; Poyner, David R; Bill, Roslyn M

20. Use of Ubp1 protease analog to produce recombinant human growth hormone in Escherichia coli - Wojtowicz-Krawiec, Anna; Sokolowska, Iwona; Smorawinska, Maria; Chojnacka-Puchta, Luiza; Mikiewicz, Diana; Lukasiewicz, Natalia; Marciniak-Rusek, Alina; Wolinowska, Renata; Bierczynska-Krzysik, Anna; Porebska, Anna Joanna; Kuthan-Styczen, Jolanta; Gurba, Lidia; Borowicz, Piotr; Mazurkiewicz, Anna; Plucienniczak, Grazyna; Plucienniczak, Andrzej

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