Recursos de colección

PubMed Central (PMC3 - NLM DTD) (2.977.438 recursos)

Archive of life sciences journal literature at the U.S. National Institutes of Health (NIH), developed and managed by NIH's National Center for Biotechnology Information (NCBI) in the National Library of Medicine (NLM).

Microbial Cell Factories

Mostrando recursos 1 - 20 de 1.139

  1. CRISPR/Cas9 mediated targeted mutagenesis of the fast growing cyanobacterium Synechococcus elongatus UTEX 2973

    Wendt, Kristen E.; Ungerer, Justin; Cobb, Ryan E.; Zhao, Huimin; Pakrasi, Himadri B.

  2. Fermentation of mixed substrates by Clostridium pasteurianum and its physiological, metabolic and proteomic characterizations

    Sabra, Wael; Wang, Wei; Surandram, Sruthi; Groeger, Christin; Zeng, An-Ping

  3. Development of an accurate kinetic model for the central carbon metabolism of Escherichia coli

    Jahan, Nusrat; Maeda, Kazuhiro; Matsuoka, Yu; Sugimoto, Yurie; Kurata, Hiroyuki

  4. Lycopene overproduction in Saccharomyces cerevisiae through combining pathway engineering with host engineering

    Chen, Yan; Xiao, Wenhai; Wang, Ying; Liu, Hong; Li, Xia; Yuan, Yingjin

  5. Maintenance-energy requirements and robustness of Saccharomyces cerevisiae at aerobic near-zero specific growth rates

    Vos, Tim; Hakkaart, Xavier D. V.; de Hulster, Erik A. F.; van Maris, Antonius J. A.; Pronk, Jack T.; Daran-Lapujade, Pascale

  6. The fed-batch principle for the molecular biology lab: controlled nutrient diets in ready-made media improve production of recombinant proteins in Escherichia coli

    Krause, Mirja; Neubauer, Antje; Neubauer, Peter
    While the nutrient limited fed-batch technology is the standard of the cultivation of microorganisms and production of heterologous proteins in industry, despite its advantages in view of metabolic control and high cell density growth, shaken batch cultures are still the standard for protein production and expression screening in molecular biology and biochemistry laboratories. This is due to the difficulty and expenses to apply a controlled continuous glucose feed to shaken cultures. New ready-made growth media, e.g. by biocatalytic release of glucose from a polymer, offer a simple solution for the application of the fed-batch principle in shaken plate and flask...

  7. Biosynthesis of selenium nanoparticles by Azoarcus sp. CIB

    Fernández-Llamosas, Helga; Castro, Laura; Blázquez, María Luisa; Díaz, Eduardo; Carmona, Manuel

  8. Enabling low cost biopharmaceuticals: high level interferon alpha-2b production in Trichoderma reesei

    Landowski, Christopher P.; Mustalahti, Eero; Wahl, Ramon; Croute, Laurence; Sivasiddarthan, Dhinakaran; Westerholm-Parvinen, Ann; Sommer, Benjamin; Ostermeier, Christian; Helk, Bernhard; Saarinen, Juhani; Saloheimo, Markku

  9. Metabolic processes of Methanococcus maripaludis and potential applications

    Goyal, Nishu; Zhou, Zhi; Karimi, Iftekhar A.
    Methanococcus maripaludis is a rapidly growing, fully sequenced, genetically tractable model organism among hydrogenotrophic methanogens. It has the ability to convert CO2 and H2 into a useful cleaner energy fuel (CH4). In fact, this conversion enhances in the presence of free nitrogen as the sole nitrogen source due to prolonged cell growth. Given the global importance of GHG emissions and climate change, diazotrophy can be attractive for carbon capture and utilization applications from appropriately treated flue gases, where surplus hydrogen is available from renewable electricity sources. In addition, M. maripaludis can be engineered to produce other useful products such as...

  10. Mob/oriT, a mobilizable site-specific recombination system for unmarked genetic manipulation in Bacillus thuringiensis and Bacillus cereus

    Wang, Pengxia; Zhu, Yiguang; Zhang, Yuyang; Zhang, Chunyi; Xu, Jianyi; Deng, Yun; Peng, Donghai; Ruan, Lifang; Sun, Ming

  11. Production in Pichia pastoris of complementary protein-based polymers with heterodimer-forming WW and PPxY domains

    Domeradzka, Natalia E.; Werten, Marc W. T.; de Vries, Renko; de Wolf, Frits A.

  12. Cellulases and beyond: the first 70 years of the enzyme producer Trichoderma reesei

    Bischof, Robert H.; Ramoni, Jonas; Seiboth, Bernhard
    More than 70 years ago, the filamentous ascomycete Trichoderma reesei was isolated on the Solomon Islands due to its ability to degrade and thrive on cellulose containing fabrics. This trait that relies on its secreted cellulases is nowadays exploited by several industries. Most prominently in biorefineries which use T. reesei enzymes to saccharify lignocellulose from renewable plant biomass in order to produce biobased fuels and chemicals. In this review we summarize important milestones of the development of T. reesei as the leading production host for biorefinery enzymes, and discuss emerging trends in strain engineering. Trichoderma reesei has very recently also been...

  13. Proof of concept for the simplified breakdown of cellulose by combining Pseudomonasputida strains with surface displayed thermophilic endocellulase, exocellulase and β-glucosidase

    Tozakidis, Iasson E. P.; Brossette, Tatjana; Lenz, Florian; Maas, Ruth M.; Jose, Joachim

  14. Engineered Zymomonas mobilis for salt tolerance using EZ-Tn5-based transposon insertion mutagenesis system

    Wang, Jing-Li; Wu, Bo; Qin, Han; You, Yang; Liu, Song; Shui, Zong-Xia; Tan, Fu-Rong; Wang, Yan-Wei; Zhu, Qi-Li; Li, Yan-Bin; Ruan, Zhi-Yong; Ma, Ke-Dong; Dai, Li-Chun; Hu, Guo-Quan; He, Ming-Xiong

  15. Recombinant Lactococcus lactis NZ9000 secretes a bioactive kisspeptin that inhibits proliferation and migration of human colon carcinoma HT-29 cells

    Zhang, Bo; Li, Angdi; Zuo, Fanglei; Yu, Rui; Zeng, Zhu; Ma, Huiqin; Chen, Shangwu

  16. Recovery of bioactive protein from bacterial inclusion bodies using trifluoroethanol as solubilization agent

    Upadhyay, Vaibhav; Singh, Anupam; Jha, Divya; Singh, Akansha; Panda, Amulya K.

  17. A new regulatory mechanism controlling carotenogenesis in the fungus Mucor circinelloides as a target to generate β-carotene over-producing strains by genetic engineering

    Zhang, Yingtong; Navarro, Eusebio; Cánovas-Márquez, José T.; Almagro, Lorena; Chen, Haiqin; Chen, Yong Q.; Zhang, Hao; Torres-Martínez, Santiago; Chen, Wei; Garre, Victoriano

  18. Lanthipeptides: chemical synthesis versus in vivo biosynthesis as tools for pharmaceutical production

    Ongey, Elvis Legala; Neubauer, Peter
    Lanthipeptides (also called lantibiotics for those with antibacterial activities) are ribosomally synthesized post-translationally modified peptides having thioether cross-linked amino acids, lanthionines, as a structural element. Lanthipeptides have conceivable potentials to be used as therapeutics, however, the lack of stable, high-yield, well-characterized processes for their sustainable production limit their availability for clinical studies and further pharmaceutical commercialization. Though many reviews have discussed the various techniques that are currently employed to produce lanthipeptides, a direct comparison between these methods to assess industrial applicability has not yet been described. In this review we provide a synoptic comparison of research efforts on total synthesis...

  19. Pichia pastoris MutS strains are prone to misincorporation of O-methyl-l-homoserine at methionine residues when methanol is used as the sole carbon source

    Schotte, Peter; Dewerte, Isabelle; De Groeve, Manu; De Keyser, Saskia; De Brabandere, Veronique; Stanssens, Patrick

  20. Recombinant Ralstonia eutropha engineered to utilize xylose and its use for the production of poly(3-hydroxybutyrate) from sunflower stalk hydrolysate solution

    Kim, Hee Su; Oh, Young Hoon; Jang, Young-Ah; Kang, Kyoung Hee; David, Yokimiko; Yu, Ju Hyun; Song, Bong Keun; Choi, Jong-il; Chang, Yong Keun; Joo, Jeong Chan; Park, Si Jae

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