Recursos de colección

PubMed Central (PMC3 - NLM DTD) (2.987.316 recursos)

Archive of life sciences journal literature at the U.S. National Institutes of Health (NIH), developed and managed by NIH's National Center for Biotechnology Information (NCBI) in the National Library of Medicine (NLM).

Microbial Cell Factories

Mostrando recursos 1 - 20 de 1.143

  1. EctD-mediated biotransformation of the chemical chaperone ectoine into hydroxyectoine and its mechanosensitive channel-independent excretion

    Czech, Laura; Stöveken, Nadine; Bremer, Erhard

  2. Versatile biocatalysis of fungal cytochrome P450 monooxygenases

    Durairaj, Pradeepraj; Hur, Jae-Seoun; Yun, Hyungdon
    Cytochrome P450 (CYP) monooxygenases, the nature’s most versatile biological catalysts have unique ability to catalyse regio-, chemo-, and stereospecific oxidation of a wide range of substrates under mild reaction conditions, thereby addressing a significant challenge in chemocatalysis. Though CYP enzymes are ubiquitous in all biological kingdoms, the divergence of CYPs in fungal kingdom is manifold. The CYP enzymes play pivotal roles in various fungal metabolisms starting from housekeeping biochemical reactions, detoxification of chemicals, and adaptation to hostile surroundings. Considering the versatile catalytic potentials, fungal CYPs has gained wide range of attraction among researchers and various remarkable strategies have been accomplished...

  3. Efficient production of 2H, 13C, 15N-enriched industrial enzyme Rhizopus chinensis lipase with native disulfide bonds

    Zhang, Meng; Yu, Xiao-Wei; Swapna, G. V. T.; Xiao, Rong; Zheng, Haiyan; Sha, Chong; Xu, Yan; Montelione, Gaetano T.

  4. Erratum to: SHuffle, a novel Escherichia coli protein expression strain capable of correctly folding disulfide bonded proteins in its cytoplasm

    Lobstein, Julie; Emrich, Charlie A.; Jeans, Chris; Faulkner, Melinda; Riggs, Paul; Berkmen, Mehmet

  5. Revisiting overexpression of a heterologous β-glucosidase in Trichoderma reesei: fusion expression of the Neosartorya fischeri Bgl3A to cbh1 enhances the overall as well as individual cellulase activities

    Xue, Xianli; Wu, Yilan; Qin, Xing; Ma, Rui; Luo, Huiying; Su, Xiaoyun; Yao, Bin

  6. Activation of a plasmid-situated type III PKS gene cluster by deletion of a wbl gene in deepsea-derived Streptomyces somaliensis SCSIO ZH66

    Huang, Huiming; Hou, Lukuan; Li, Huayue; Qiu, Yanhong; Ju, Jianhua; Li, Wenli

  7. CRISPR/Cas9 mediated targeted mutagenesis of the fast growing cyanobacterium Synechococcus elongatus UTEX 2973

    Wendt, Kristen E.; Ungerer, Justin; Cobb, Ryan E.; Zhao, Huimin; Pakrasi, Himadri B.

  8. Fermentation of mixed substrates by Clostridium pasteurianum and its physiological, metabolic and proteomic characterizations

    Sabra, Wael; Wang, Wei; Surandram, Sruthi; Groeger, Christin; Zeng, An-Ping

  9. Development of an accurate kinetic model for the central carbon metabolism of Escherichia coli

    Jahan, Nusrat; Maeda, Kazuhiro; Matsuoka, Yu; Sugimoto, Yurie; Kurata, Hiroyuki

  10. Lycopene overproduction in Saccharomyces cerevisiae through combining pathway engineering with host engineering

    Chen, Yan; Xiao, Wenhai; Wang, Ying; Liu, Hong; Li, Xia; Yuan, Yingjin

  11. Maintenance-energy requirements and robustness of Saccharomyces cerevisiae at aerobic near-zero specific growth rates

    Vos, Tim; Hakkaart, Xavier D. V.; de Hulster, Erik A. F.; van Maris, Antonius J. A.; Pronk, Jack T.; Daran-Lapujade, Pascale

  12. The fed-batch principle for the molecular biology lab: controlled nutrient diets in ready-made media improve production of recombinant proteins in Escherichia coli

    Krause, Mirja; Neubauer, Antje; Neubauer, Peter
    While the nutrient limited fed-batch technology is the standard of the cultivation of microorganisms and production of heterologous proteins in industry, despite its advantages in view of metabolic control and high cell density growth, shaken batch cultures are still the standard for protein production and expression screening in molecular biology and biochemistry laboratories. This is due to the difficulty and expenses to apply a controlled continuous glucose feed to shaken cultures. New ready-made growth media, e.g. by biocatalytic release of glucose from a polymer, offer a simple solution for the application of the fed-batch principle in shaken plate and flask...

  13. Enabling low cost biopharmaceuticals: high level interferon alpha-2b production in Trichoderma reesei

    Landowski, Christopher P.; Mustalahti, Eero; Wahl, Ramon; Croute, Laurence; Sivasiddarthan, Dhinakaran; Westerholm-Parvinen, Ann; Sommer, Benjamin; Ostermeier, Christian; Helk, Bernhard; Saarinen, Juhani; Saloheimo, Markku

  14. Metabolic processes of Methanococcus maripaludis and potential applications

    Goyal, Nishu; Zhou, Zhi; Karimi, Iftekhar A.
    Methanococcus maripaludis is a rapidly growing, fully sequenced, genetically tractable model organism among hydrogenotrophic methanogens. It has the ability to convert CO2 and H2 into a useful cleaner energy fuel (CH4). In fact, this conversion enhances in the presence of free nitrogen as the sole nitrogen source due to prolonged cell growth. Given the global importance of GHG emissions and climate change, diazotrophy can be attractive for carbon capture and utilization applications from appropriately treated flue gases, where surplus hydrogen is available from renewable electricity sources. In addition, M. maripaludis can be engineered to produce other useful products such as...

  15. Mob/oriT, a mobilizable site-specific recombination system for unmarked genetic manipulation in Bacillus thuringiensis and Bacillus cereus

    Wang, Pengxia; Zhu, Yiguang; Zhang, Yuyang; Zhang, Chunyi; Xu, Jianyi; Deng, Yun; Peng, Donghai; Ruan, Lifang; Sun, Ming

  16. Production in Pichia pastoris of complementary protein-based polymers with heterodimer-forming WW and PPxY domains

    Domeradzka, Natalia E.; Werten, Marc W. T.; de Vries, Renko; de Wolf, Frits A.

  17. Cellulases and beyond: the first 70 years of the enzyme producer Trichoderma reesei

    Bischof, Robert H.; Ramoni, Jonas; Seiboth, Bernhard
    More than 70 years ago, the filamentous ascomycete Trichoderma reesei was isolated on the Solomon Islands due to its ability to degrade and thrive on cellulose containing fabrics. This trait that relies on its secreted cellulases is nowadays exploited by several industries. Most prominently in biorefineries which use T. reesei enzymes to saccharify lignocellulose from renewable plant biomass in order to produce biobased fuels and chemicals. In this review we summarize important milestones of the development of T. reesei as the leading production host for biorefinery enzymes, and discuss emerging trends in strain engineering. Trichoderma reesei has very recently also been...

  18. Proof of concept for the simplified breakdown of cellulose by combining Pseudomonasputida strains with surface displayed thermophilic endocellulase, exocellulase and β-glucosidase

    Tozakidis, Iasson E. P.; Brossette, Tatjana; Lenz, Florian; Maas, Ruth M.; Jose, Joachim

  19. Engineered Zymomonas mobilis for salt tolerance using EZ-Tn5-based transposon insertion mutagenesis system

    Wang, Jing-Li; Wu, Bo; Qin, Han; You, Yang; Liu, Song; Shui, Zong-Xia; Tan, Fu-Rong; Wang, Yan-Wei; Zhu, Qi-Li; Li, Yan-Bin; Ruan, Zhi-Yong; Ma, Ke-Dong; Dai, Li-Chun; Hu, Guo-Quan; He, Ming-Xiong

  20. Recombinant Lactococcus lactis NZ9000 secretes a bioactive kisspeptin that inhibits proliferation and migration of human colon carcinoma HT-29 cells

    Zhang, Bo; Li, Angdi; Zuo, Fanglei; Yu, Rui; Zeng, Zhu; Ma, Huiqin; Chen, Shangwu

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