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PubMed Central (PMC3 - NLM DTD) (2,795,904 recursos)
Archive of life sciences journal literature at the U.S. National Institutes of Health (NIH), developed and managed by NIH's National Center for Biotechnology Information (NCBI) in the National Library of Medicine (NLM).

Microbial Cell Factories

Mostrando recursos 1 - 20 de 943

1. Dynamic control of ERG9 expression for improved amorpha-4,11-diene production in Saccharomyces cerevisiae - Yuan, Jifeng; Ching, Chi-Bun

2. An ancient Chinese wisdom for metabolic engineering: Yin-Yang - Wu, Stephen G; He, Lian; Wang, Qingzhao; Tang, Yinjie J
In ancient Chinese philosophy, Yin-Yang describes two contrary forces that are interconnected and interdependent. This concept also holds true in microbial cell factories, where Yin represents energy metabolism in the form of ATP, and Yang represents carbon metabolism. Current biotechnology can effectively edit the microbial genome or introduce novel enzymes to redirect carbon fluxes. On the other hand, microbial metabolism loses significant free energy as heat when converting sugar into ATP; while maintenance energy expenditures further aggravate ATP shortage. The limitation of cell “powerhouse” prevents hosts from achieving high carbon yields and rates. Via an Escherichia coli flux balance analysis...

3. A quorum sensing-based in vivo expression system and its application in multivalent bacterial vaccine - Chu, Teng; Ni, Chunshan; Zhang, Lingzhi; Wang, Qiyao; Xiao, Jingfan; Zhang, Yuanxing; Liu, Qin

4. Serine protease inhibitors protect better than IL-10 and TGF-β anti-inflammatory cytokines against mouse colitis when delivered by recombinant lactococci - Bermúdez-Humarán, Luis G; Motta, Jean-Paul; Aubry, Camille; Kharrat, Pascale; Rous-Martin, Laurence; Sallenave, Jean-Michel; Deraison, Céline; Vergnolle, Nathalie; Langella, Philippe

5. Microbial host selection affects intracellular localization and activity of alcohol-O-acetyltransferase - Zhu, Jie; Lin, Jyun-Liang; Palomec, Leidy; Wheeldon, Ian

6. Bioprocess automation on a Mini Pilot Plant enables fast quantitative microbial phenotyping - Unthan, Simon; Radek, Andreas; Wiechert, Wolfgang; Oldiges, Marco; Noack, Stephan

7. Annual acknowledgement of manuscript reviewers - Villaverde, Antonio
The Editors of Microbial Cell Factories would like to thank all our reviewers who have contributed to the journal in Volume 13 (2014).

8. Heterologous expression of a recombinant lactobacillal β-galactosidase in Lactobacillus plantarum: effect of different parameters on the sakacin P-based expression system - Nguyen, Tien-Thanh; Nguyen, Hoang-Minh; Geiger, Barbara; Mathiesen, Geir; Eijsink, Vincent GH; Peterbauer, Clemens K; Haltrich, Dietmar; Nguyen, Thu-Ha

9. Metabolic engineering of Escherichia coli for enhanced arginine biosynthesis - Ginesy, Mireille; Belotserkovsky, Jaroslav; Enman, Josefine; Isaksson, Leif; Rova, Ulrika

10. Effect of plasmid replication deregulation via inc mutations on E. coli proteome & simple flux model analysis - Meade, Jonathan; Bartlow, Patrick; Trivedi, Ram Narayan; Akhtar, Parvez; Ataai, Mohammad M; Khan, Saleem A; Domach, Michael M
When the replication of a plasmid based on sucrose selection is deregulated via the inc1 and inc2 mutations, high copy numbers (7,000 or greater) are attained while the growth rate on minimal medium is negligibly affected. Adaptions were assumed to be required in order to sustain the growth rate. Proteomics indicated that indeed a number of adaptations occurred that included increased expression of ribosomal proteins and 2-oxoglutarate dehydrogenase. The operating space prescribed by a basic flux model that maintained phenotypic traits (e.g. growth, byproducts, etc.) within typical bounds of resolution was consistent with the flux implications of the proteomic changes.

11. Process development for the production of 15β-hydroxycyproterone acetate using Bacillus megaterium expressing CYP106A2 as whole-cell biocatalyst - Kiss, Flora M; Lundemo, Marie T; Zapp, Josef; Woodley, John M; Bernhardt, Rita

12. Genome reduction boosts heterologous gene expression in Pseudomonas putida - Lieder, Sarah; Nikel, Pablo I; de Lorenzo, Víctor; Takors, Ralf

13. Ag43-mediated display of a thermostable β-glucosidase in Escherichia coli and its use for simultaneous saccharification and fermentation at high temperatures - Muñoz-Gutiérrez, Iván; Moss-Acosta, Cessna; Trujillo-Martinez, Berenice; Gosset, Guillermo; Martinez, Alfredo

14. The use of fosmid metagenomic libraries in preliminary screening for various biological activities - Felczykowska, Agnieszka; Dydecka, Aleksandra; Bohdanowicz, Małgorzata; Gąsior, Tomasz; Soboň, Marek; Kobos, Justyna; Bloch, Sylwia; Nejman-Faleńczyk, Bożena; Węgrzyn, Grzegorz

15. Characterization of an Ebosin derivative produced by heterologous gene replacement in Streptomyces sp. 139 - Zhang, Yang; Shan, Junjie; Bao, Yonggang; Bai, Liping; Jiang, Rong; Guo, Lianhong; Yao, Chen; Zhang, Ren; Li, Yuan

16. A recombinant CYP11B1 dependent Escherichia coli biocatalyst for selective cortisol production and optimization towards a preparative scale - Schiffer, Lina; Anderko, Simone; Hobler, Anna; Hannemann, Frank; Kagawa, Norio; Bernhardt, Rita

17. Synthetic signal sequences that enable efficient secretory protein production in the yeast Kluyveromyces marxianus - Yarimizu, Tohru; Nakamura, Mikiko; Hoshida, Hisashi; Akada, Rinji

18. A single freeze-thawing cycle for highly efficient solubilization of inclusion body proteins and its refolding into bioactive form - Qi, Xingmei; Sun, Yifan; Xiong, Sidong

19. Construction of efficient xylose utilizing Pichia pastoris for industrial enzyme production - Li, Pengfei; Sun, Hongbing; Chen, Zao; Li, Yin; Zhu, Taicheng

20. High yield purification of full-length functional hERG K+ channels produced in Saccharomyces cerevisiae - Molbaek, Karen; Scharff-Poulsen, Peter; Helix-Nielsen, Claus; Klaerke, Dan A; Pedersen, Per Amstrup
The hERG potassium channel is essential for repolarization of the cardiac action potential. Due to this vital function, absence of unintended and potentially life-threatening interactions with hERG is required for approval of new drugs. The structure of hERG is therefore one of the most sought-after. To provide purified hERG for structural studies and new hERG biomimetic platforms for detection of undesirable interactions, we have developed a hERG expression platform generating unprecedented amounts of purified and functional hERG channels. Full-length hERG, with or without a C-terminally fused green fluorescent protein (GFP) His 8-tag was produced from a codon-optimized hERG cDNA in...

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