PubMed Central (PMC3 - NLM DTD)
(2.081.148 recursos)
Archive of life sciences journal literature at the U.S. National Institutes of Health (NIH), developed and managed by NIH's National Center for Biotechnology Information (NCBI) in the National Library of Medicine (NLM).
10.
SF-1 a key player in the development and differentiation of steroidogenic tissues - Val, Pierre; Lefrançois-Martinez, Anne-Marie; Veyssière, Georges; Martinez, Antoine
Since its discovery in the early 1990s, the orphan nuclear receptor SF-1 has been attributed a central role in the development and differentiation of steroidogenic tissues. SF-1 controls the expression of all the steroidogenic enzymes and cholesterol transporters required for steroidogenesis as well as the expression of steroidogenesis-stimulating hormones and their cognate receptors. SF-1 is also an essential regulator of genes involved in the sex determination cascade. The study of SF-1 null mice and of human mutants has been of great value to demonstrate the essential role of this factor in vivo, although the complete adrenal and gonadal agenesis in...
13.
ERβ Binds N-CoR in the Presence of Estrogens via an LXXLL-like Motif in the N-CoR C-terminus - Webb, Paul; Valentine, Cathleen; Nguyen, Phuong; Price, Richard H; Marimuthu, Adhirai; West, Brian L; Baxter, John D; Kushner, Peter J
Nuclear receptors (NRs) usually bind the corepressors N-CoR and SMRT in the absence of ligand or in the presence of antagonists. Agonist binding leads to corepressor release and recruitment of coactivators. Here, we report that estrogen receptor β (ERβ) binds N-CoR and SMRT in the presence of agonists, but not antagonists, in vitro and in vivo. This ligand preference differs from that of ERα interactions with corepressors, which are inhibited by estradiol, and resembles that of ERβ interactions with coactivators. ERβ /N-CoR interactions involve ERβ AF-2, which also mediates coactivator recognition. Moreover, ERβ recognizes a sequence (PLTIRML) in the N-CoR...
18.
Establishment of a monoclonal antibody for human LXRα: Detection of LXRα protein expression in human macrophages - Watanabe, Yuichiro; Tanaka, Toshiya; Uchiyama, Yasutoshi; Takeno, Tetsu; Izumi, Akashi; Yamashita, Hisahiko; Kumakura, Junko; Iwanari, Hiroko; Shu-Ying, Jiang; Naito, Makoto; Mangelsdorf, David J; Hamakubo, Takao; Kodama, Tatsuhiko
Liver X activated receptor alpha (LXRα) forms a functional dimeric nuclear receptor with RXR that regulates the metabolism of several important lipids, including cholesterol and bile acids. As compared with RXR, the LXRα protein level in the cell is low and the LXRα protein itself is very hard to detect. We have previously reported that the mRNA for LXRα is highly expressed in human cultured macrophages. In order to confirm the presence of the LXRα protein in the human macrophage, we have established a monoclonal antibody against LXRα, K-8607. The binding of mAb K-8607 to the human LXRα protein was...
1.
