Regeneration in Zebrafish (Danio rerio) fins: pattern of expression of mineralizationârelated Gla proteins - Brito, A. B.; Gavaia, Paulo J.; Cancela, Leonor
Teleost fishes have the exceptional ability to largely regenerate severed appendages
comprising several different tissues. Fin regeneration starts through the formation of
heterogeneous mesenchyma-like cells, named blastema, and located between stump
tissues and the wounded epidermis. This event, denominated epimorphic regeneration,
comprises strict growth control and cell reprogramming leading to faithful restoration
of the lost parts. Matrix Gla Protein (Mgp) and Bone Gla Protein (Bgp, osteocalcin)
are small extracellular matrix Gla proteins, members of the vitamin K-dependent
Rearing fish larvae for extensive and semi-intensive aquaculture - the ânaturalâ mesocosms system - Cunha, M. E.; Quental-Ferreira, H.; Boglione, C.; Palamara, E.; Gavaia, Paulo J.; PousÃ£o-Ferreira, P.
Non-intensive aquaculture systems are rapidly
generating interest due to increased awareness of consumers about environmental issues, including those associated with coastal areas; eutrophication as a result
of intensive aquaculture production is seen as a problem, there is concern about animal welfare issues in fish rearing, and food safety is of course a major concern.
Factors enhancing fish sperm quality and emerging tools for sperm analysis - Cabrita, E.; Martínez-Páramo, S.; Gavaia, Paulo J.; Riesco, M. F.; Valcarce, D. G.; Sarasquete, C.; Herráez, M. P.; Robles, V.
With this review we try to give a comprehensive overview of the current methods used in research to assess
sperm quality. In addition, we identify some of the most important factors for enhancing sperm production
and quality, including, broodstock nutrition, epigenetics and sperm management (cryopreservation). Sperm
quality can be assessed by analyzing different parameters fromsimplemethods to very sophisticated approaches
involving molecular tools. Parameters related with sperm composition or function (e.g. spermatozoa plasma
membrane lipids, seminal plasma composition,motility activation) have successfully characterized a spermsample
but could not respond to the causes behind sperm defects. Reactive oxygen species (ROS) are one of the
causes of the impairment of sperm...
Spectroscopic characterization of a novel 2×[4Fe–4S] ferredoxin isolated from Desulfovibrio desulfuricans ATCC 27774 - Rodrigues, Pedro; Moura, Isabel; Macedo, Anjos L.; Moura, José J. G.
A novel iron /sulfur containing protein, a ferredoxin (Fd), was purified to homogeneity from the extract of Desulfovibrio desulfuricans American type culture collection (ATCC) 27774. The purified protein is a 13.4 kDa homodimer with a polypeptide chain of 60 amino acids residues, containing eight cysteines that coordinate two [4Fe /4S] clusters. The protein is shown to be air sensitive and cluster conversions take place. We structurally characterize a redox state that contains two [4Fe /4S] cores. 1D and 2D
1H NMR studies are reported on form containing the clusters in the oxidized state. Based on the nuclear Overhauser effect (NOE), relaxation...
Effect of antiprotozoal drugs on the proliferation of the bivalve parasite Perkinsus olseni - Elandalloussi, Laurence M.; Leite, Ricardo B.; Rodrigues, Pedro; Afonso, Ricardo; Nunes, Patrícia A.; Cancela, Leonor
The protozoan parasite Perkinsus olseni causes severe losses among Ruditapes decussatus clams. The development of an in
vitro culture of this parasite together with the use of a proliferation assay has provided the opportunity to screen for drug
sensitivity of this parasite. Xenobiotics known for their antimalarial and antiprotozoal properties were tested against P. olseni. Only four of these drugs, namely cycloheximide, pyrimethamine, deferoxamine (DFO) and 2,2-bipyridyl (BIP), showed in vitro inhibitory effect on the parasite proliferation. Two in vivo experiments were designed to determine the effect of iron chelators on reducing P. olseni infection in clams. For this purpose, naturally infected...
Influence of supplemental maslinic acid (olive-derived triterpene) on the post-mortem muscle properties and quality traits of gilthead seabream - Matos, Elisabete; Silva, Tomé S.; Wulff, Tune; Valente, Luisa M. P.; Sousa, Vera; Sampaio, Eduardo; Gonçalves, Amparo; Silva, Joana M. G.; Dinis, Maria Teresa; Rodrigues, Pedro; Dias, J.
Maslinic acid, a natural triterpene, was evaluated as a dietary supplement to modulate glycogen post-mortem mobilization in gilthead seabream muscle. For this purpose, a multidisciplinary trial was undertaken, where flesh quality criteria, as well as biochemical and histological parameters, enzymatic activities and protein expression in the muscle were assessed. Supplementing gilthead seabream diets with maslinic acid mainly resulted in hypertrophy of muscle fibres and inhibition of cathepsin B activity, with no observed differences
in terms of glycogen and ATP content of the muscle, as well as glycogen phosphorylase activity. Proteomic analysis showed a low impact of maslinic acid supplementation on muscle...
Photochemistry of 1-allyl-4-aryltetrazolones in solution; structural effects on photoproduct selectivity - Ismael, A.; Serpa, C.; Cristiano, Maria Lurdes Santos
The photochemistry of tetrazolones derived from the carbocyclic allylic alcohols cyclohex-2-enol and 3-methylcyclohex-2-enol and from the natural terpene alcohol nerol was investigated in solution with the
aim of assessing the effect of solvent and of structural constraints imposed by bulky allylic moieties on photoproduct selectivity and stability. Photolysis of tetrazolones derived from nerol and cyclohex-2-enol
afforded the corresponding pyrimidinones as major products through a pathway that appears to be similar to that proposed for other 1-allyl-4-phenyl-1,4-dihydro-5H-tetrazol-5-ones derived from acyclic and
unhindered allylic alcohols previously investigated but photolysis of the tetrazolone derived from the bulkier 3–methylcyclohex-2-enol 4c leads to formation of a benzimidazolone, indicating...
Vertebral deformities and local accumulation of gla proteins in sparus aurata - Roberto, V.; Gavaia, Paulo J.; Cancela, Leonor
Development of skeletal deformities is a frequent problem in Mediterranean fish aquaculture. Investigation of proteins
involved in tissue calcification may help us uncover a possible correlation between the appearance of skeletal deformities
and a defect in the site of expression and/or accumulation of a particular gene or protein. The purpose of this study was
to investigate if the presence of skeletal deformities was related with any changes in the sites of osteocalcin (Oc or Bgp
â Bone Gla Protein) and matrix Gla protein (Mgp) accumulation using immunohistochemistry methods.
Two-dimensional proteomics as a tool to evaluate nutritional effects in farmed fish - Richard, N.; Gavaia, Paulo J.; De Vareilles, M.; Silva, Tomé S.; Cordeiro, O.; Rodrigues, Pedro; Conceição, L. E. C.
The use of proteomics in fish research is at a relatively early stage compared to terrestrial vertebrates. It has already been applied in the field of fish nutrition, enabling to point out metabolic changes occurring in response to dietary manipulations such as a variation in energy content, dietary nitrogen compostion, lipid composition or the incorporation of plant protein sources in aquafeeds.
Skeletal quality in Senegalese sole: characterization of fish resulting from environmental and nutritional trials - Gavaia, Paulo J.; DionÃsio, G.; Cardeira, J.; Cunha, M. E.; PousÃ£o-Ferreira, P.; ConceiÃ§Ã£o, L. E. C.; Cancela, Leonor
The Senegalese sole (Solea senegalensis) is characteristic from southern Europe and
Mediterranean. It has recently been adapted for aquaculture production since it is well accepted by
consumers and reaches high commercial values. After the initial description of the ontogenic
events of skeletogenesis, systematic evaluation of the incidence of skeletal malformations by
several groups revealed high levels of skeletal deformities reaching up to 90% of the population.
Skeletal development and performance of zebrafish Danio rerio (Hamilton, 1822) larvae and juvenile fish fed with different diets - Cardeira da Silva, J.; DionÃsio, G.; Bensimon-Brito, A.; Cancela, Leonor; Gavaia, Paulo J.
The appearance of skeletal deformities is a major problem associated to intensive, large-scale culture of fish
species, affecting growth, development and ultimately, survival of produced fish. Although the mechanisms
working behind it are still unclear, it is known that environmental, genetic and nutritional factors play
fundamental roles. Given its importance as a vertebrate model to study skeleton-related diseases, the selection
of an appropriate dietary protocol is a critical step in order to diminish the nutritional effect on bone and
Skeletal development and bone-related gene expression in fish larvae - Gavaia, Paulo J.; Cancela, Leonor
The fish skeleton; skeletal malformations; skeletal malformations in mediterranean species; Types of skeletal malformations; Senegal sole skeletal development; Senegal sole skeletal deformities; Skeletal development in Danio rerio; ALP and TRAP in skeletal development; Skeletal development in Pagrus auriga; Time course of skeletal development in Pagrus auriga; Spatio-temporal pattern of bgp expression; bgp / mgp expression during development; In situ localization of zebrafish bgp mRNA;In situ localization of sole bgp mRNA; Immuno-detection of Bgp accumulation in zebrafish; Immuno-detection of Bgp accumulation in sole
P.; In situ localization of zebrafish and sole mgp mRNA; Immuno-detection of MGP accumulation in zebrafish; Summary of zebrafish...
Identification of bone gla protein during early development of Solea Senegalensis - Gavaia, Paulo J.; Faustino, M.; Cancela, Leonor
With the objective of better understanding the calcification processus in the Sole (Solea senegalensis) we have followed the appearance of cartilage and bone structures during the sole early developmental stages (from hatching till 51 days post-Hatching-DPH) by a specific double labelling method (Alcin blue/Alizarin red).