Ahmed, Jameel; Nirmal, Sunil A.; Dhasade, Vipul V.; Mandal, Subhash C.
The present study deals with the amelioration by Delonix regia aerial parts extract against
hepatotoxicity induced by carbon tetrachloride (CCl4), which was evaluated in terms of serum marker enzymes
like aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, albumine, total
protein, direct bilirubine and total bilirubine. These biochemical parameters were alter by the single dose
of CCl4 (2 ml/kg, p.o.). Pretreatment with D. regia extracts prior to administration of CCl4, at the dose of
400 mg/kg p.o. per day for 7 days, significantly restored all the serum and liver tissue parameters near to
the normal levels. Silymarine was used as reference standard. Amongst all the fractions, ethyl acetate...
Araujo, Gabriel L. B.; Salvio Neto, Helio; Zaim, Márcio H.; Matos, Jivaldo R.
Powder mixtures (1:1) of tibolone polymorphic forms I (monoclinic) and II (triclinic) and excipients have been prepared and compacted. The samples were stored at 50 °C and 90% RH for one month and subsequently were evaluated using differential scanning calorimetry (DSC) and high-performance
liquid chromatography (HPLC). The results indicate that during the compaction, the applied pressure
reduced the chemical stability of tibolone in both polymorph forms. The triclinic form was more chemically unstable, both pure and in contact with excipients, than the monoclinic form. Lactose monohydrate
was shown to reduce chemical degradation for both forms. Ascorbyl palmitate was shown to affect
the tibolone stability...
Upadhyaya, Sunil Datt; Nayak, Preeti Sagar; Upadhyaya, Anubha
A simple procedure was described for the determination of Withanolide A and Withanolide B in Ashwagandha root by HPLC. Separation was achieved on a Spherisorb C18 column with Water: Acetonitrile in the gradient elution. Detection was accomplished with UV detection at λ = 216 nm. Using this method, the bioactive steroid in Withania somnifera was determined. Significant variations in the contents of Withanolide A and Withanolide B in Ashwagandha root growing in different locations were also observed.
Costa, José G.M. da; Santos, Paula F. dos; Brito, Samara A.; Rodrigues, Fabíola F.G.; Coutinho, Henrique D.M.; Botelho, Marco A.; Lima, Sidney G. de
Aedes aegypti L. é um dos vetores do dengue. No Brasil, tem ganhado muita atenção no setor da saúde pública, uma vez que esta doença tem se tornando mais agressiva na forma hemorrágica na popula- ção. Esse estudo teve como objetivo investigar o efeito de óleos essenciais de Piper aduncum, Piper marginatum e Piper nigrum contra larvas de Aedes aegypti. Em um esforço para encontrar uma maneira natural, eficaz e acessível para controlar esta doença endêmica, as atividades dos óleos essenciais, a partir das plantas, foram analisadas por comparação através da medida da CL50 . Os óleos essenciais obtidos por...
Gámez, Rafael; Gutiérrez, Ariadne; Más, Rosa; Arencibia, Daniel; Curveco, Dayisell; García, Haydée
D-002 is a mixture of 6 high molecular weight primary alcohols purified from beeswax with anti-inflammatory and antioxidant effects. This study investigated the in vitro genotoxic potential of D-002 to produce chromosomal aberrations (CA) in peripheral blood lymphocytes. Fresh suspensions of BWA were added (500; 750, 1000, 2500 and 5000 μg/mL) to cultures with (microsomal liver fraction S9 mix) or without metabolic activation. Concurrent negative (Tween/water vehicle) and positive controls (cyclophosphamide 15 μg/mL and mitomicyn C (0.3 μg/mL in the tests with and without metabolic activation, respectively) were included. Two hundred (200) metaphases by group were examined and the numbers...
Vhuiyan, Mynol I.; Rahman, Mohammad S.; Kaisar, Mohammad A.; Hasan, Choudhury M.; Rashid, Mohammad A.
The methanolic extract of the whole plant of Oxalis corniculata was evaluated for antioxidant and membrane stabilizing activities. The total phenolics in the methanol extract was found 213.69 mg of gallic acid per g of extract when determined using the Folin-Ciocalteu reagent. Besides, antioxidant activity was also assessed by various methods, including free radical, hydrogen peroxide, and nitric oxide scavenging and phosphomolybdenum antioxidant assay and reducing power determination. In these cases, butylated hydroxytoluene (BHT) and ascorbic acid were used as a standard antioxidants. The membrane stabilizing activity was assessed by using erythrocyte in hypotonic solution, using indomethacin as the standard....
Boligon, Aline A.; Janovik, Vanessa; Feltrin, Andriéli C.; Machado, Michel; Frohlich, Janaína K.; Athayde, Margareth L.
Fracionamento cromatográfico da fração em diclorometano obtida do extrato etanólico das cascas do tronco de Scutia buxifolia Reissek (Rhamnaceae) conduziu ao isolamento de lupeol, β-sitosterol e estigmasterol. As estruturas foram identificadas através de técnicas espectroscópicas usuais, além da comparação com dados relatados na literatura. Os compostos isolados são relatados pela primeira vez para a espécie S. buxifolia.
Rossato, Angela E.; Schenkel, Eloir Paulo
Os procedimentos relacionados com o processo de Seleção e Padronização de Medicamentos foram analisados em 25 Farmácias Hospitalares dos Hospitais do sul do estado de Santa Catarina – Brasil.
Os dados foram coletados através de questionário estruturado, aplicado in loco na forma de entrevista e posteriormente consolidados pela abordagem metodológica do Discurso do Sujeito Coletivo. Os resultados apontam que nenhum dos hospitais dispõe de Comissão de Farmácia e Terapêutica e em torno de 70% dos hospitais a escolha dos medicamentos é realizada exclusivamente pelos médicos. Em relação à prescrição de medicamentos não padronizados, em 80 % dos casos prevalece o parecer...
Yajima, Edson M.; Zanin, Gisella M.; Albuquerque Cavalcanti, Osvaldo
Hidrocolóides têm sido amplamente estudados no desenvolvimento de novos materiais voltados a aplicação em sistemas para liberação modificada de fármacos, especialmente em função das características de biodegradabilidade e ampla variedade de estruturas e propriedades. Todavia muitos desses materiais apresentam elevada hidrossolubilidade, podendo quando aplicados na condição de carreadores de fármaco, promover liberação prematura do conteúdo. Esta limitação encontra na reticulação química alternativa promissora tanto em nível terapêutico quanto industrial. Neste trabalho foram estudadas as propriedades térmicas de filmes isolados de gelatina, quitosana e suas associações, reticulados com genipina, através das técnicas de termogravimetria (TG) e calorimetria exploratória diferencial (DSC). Os...
Teoh, Magdalene; Radhakrishnan, Shantini; Moo, Kai S.; Narayanan, Prasad; Bukhari, Nadeem I.; Segarra, Ignacio
Imatinib inhibits Bcr-Abl, c-KIT and PDGFR kinases involved in chronic myelogenous leukemia and gastrointestinal stromal tumors. Mice were given imatinib PO (50 mg/kg) or IV (12.5 mg/kg) and plasma, liver, brain, spleen, kidney disposition profiles analyzed. Plasma t1/2 was 4.5 h. IV plasma AUC0→∞ was 11.59 μg·h/mL, MRT was 4.87 h, Cl was 1.08 l/h/kg and VSS was 5.23 l/kg. PO AUC0→∞ was 12.82 μg·h/mL, MRT 5.1 h, CMAX was 6.99 ± 2.84 μg/mL, absorption rate constant, Ka was 4.348 h–1, bioavailability was 27.7%, VSS was 5.51 l/kg. The hepatic extraction ratio was 0.384 and the minimum dose fraction absorbed...
Necchi, Raquel M.M.; Zanetti, Gilberto Dolejal; Maki, Tiago D.T.; Royer, Lauri A.J.; Manfron, Melânia Palermo
Morphoanathomical parameters, for macroscopic and microscopic analyses of the cladodes
of Nopalea cochenillifera, plant recognized traditionally like medicinal, allow his authenticity. The phylloclades
have wavyness and areoles with three straight and hyaline thorns. The uniseriate epidermis has
parallelocytic stomata and mucilaginous cells, followed by an annular collenchyma and a palisade
parenchyma on average with eight cell layers. The vascular region is constituted by isolated vascular bundles
of the open collateral type. The central cylinder is composed by a parenchymatic medulla. The mucilage
structures and the druses of calcium oxalate are present in cortical region in and central cylinder.
They are in bigger number near to the vascular...
Dias, Ejussara B.; Santos, Gabriella P.; Gleizer M.B., Vieira; Rezende, Ana M.B.; Boechat, Giovanna A.P.; Simões, Ângela M.C.; Andrade, Tadeu U.
No posee resumen.
Tang, Daoquan; Gao, Yuanyuan; Tang, Anbang; Chen, Yonggang; Wei, Yaqin; Yin, Xiaoxin
Diabetes mellitus (DM) is a serious health problem affecting millions of individuals worldwide.
It is showed that some changes of many enzymes and transporters concerned with metabolism and
disposal of drug have taken place in organism under pathologic state of DM. The pharmacokinetic of drug
should be different between diabetic and normal animals. Rutin and quercetin can also be used to treatment
of diabetic mellitus. So, this paper investigated the difference of pharmacokinetic profiles of rutin
and quercetin in diabetic and normal rats in vivo by HPLC-DAD method. The pharmacokinetic parameters
were analyzed by double-compartmental method (DAS2.0). The pharmacokinetic parameters of rutin
in normal and diabetic rats...
Huang, Xianxiao; Gao, Wenyuan; Man, Shuli; Gao, Ying; Huang, Luqi; Liu, Changxiao
Twelve compounds were isolated from the rhizoma of Paris polyphylla Smith var. pubescens Handel-Mazzetti. Based on spectral data, the isolated compounds were identified as stigmasterol (1), β- Ecdysterone (2), Diosgenin (3), Ophiopogonin C’ (4), Diosgenin-3-O-α-L-arabinofuranosyl(1→4)-β-D-glucoside (5), Dioscin (6), Paris saponin I (7), Paris saponin II (8), Pennogenin-3-O-α-L- arabinofuranosyl (1→4)- β- D-glucoside (9), Paris saponin VI (10), Pennogennin- 3-O-α-L-arabinofuranosyl(1→4)-[α-Lrhamnopyranosyl(1→2)]-β-D-glucoside (11) and Paris saponin VII (12). Their cytotoxicity and anti-migration on mouse B16 melanoma cells were evaluated.
Kshirsagar, Sanjay J.; Bhalekar, Mangesh R.; Madgulkar, Ashwini R.; Sable, Preeti N.; Gupta, Bijan Kumar
The aim of the present investigation is to improve the dissolution of poorly water soluble drug valsartan by preparing solid dispersions and also to evaluate the effect of different inert carriers on flow properties of solid dispersion. Valsartan is a poorly soluble drug useful in the treatment of hypertension. Absorption window of valsartan is stomach and upper part of small intestine. One possible way to improve dissolution rate is solid dispersions of the drug. The solid dispersions were prepared by solvent evaporation method using HPMC E5 LV as water soluble carrier, as use of HPMC low viscosity polymers for solid...
Mila C., Loreley; Valdes, Rodolfo; Padilla, Sigifredo; Mendoza, Otto; Gomez, Leonardo; García A., Cristina; Geada, Déborah; Ferro, Williams; Pujol, Merardo; Tamayo, Andrés; Enriquez, Gil; Álvarez, Tatiana; Sanchez, Rafael; Brito, José
The production of biopharmaceuticals by transgenic plants is a promising choice to achieve the multi-kilogram amount of products needed to treat many human diseases. However, this scientific field is still lacking of approved specific guidelines regarding points to consider for manufacturing and application of these products. In such sense, the implementation of new manufacturing processes and quality systems using the quality risks management is recognized as something of prime importance in the current pharmaceutical industry. In this work, we summarize the application of the FMEA method to design the manufacturing process of a plantibody, employed in the hepatitis B vaccine...
Magina, Michele A.; Gilioli, Andressa; Moresco, Henrique H.; Colla, Guilherme; Pizzolatti, Moacir Geraldo; Brighente, Inês Maria Costa
A atividade antioxidante dos extratos vegetais de Eugenia brasiliensis, E. beaurepaireana e E. umbelliflora foi avaliada usando diferentes métodos: Determinação da ação sequestrante de radicais livres usando DPPH, determinação do potencial redutor e determinação do potencial inibidor da peroxidação lipídica. Foi correlacionado também a o conteúdo de fenólicos totais e de flavonóides contidos nestes extratos e frações com a atividade antioxidante. As espécies E. brasiliensis e E. beaurepaireana mostraram maior capacidade antioxidante em relação a todos os métodos utilizados. Estas espécies exibiram também maior teor de compostos fenólicos. Uma significante relação entre a atividade antioxidante e o conteúdo de fenólicos...
Tian, Yongzhi; Wang, Jianjie; Li, Qingwang; Ou, Yetao; Li, Chunjiang; Sun, Xiaoxin
Paeonol is a micromolecular phenolic compound and it is the main component of Chinese
herbal medicine that has been isolated from the root bark of Paeonia moutan. Paeonol is identified to
have various physiological activities. In this study the antitumor activity and the possible mechanisms of
paeonol were investigated in mice bearing EMT6 breast cancer model. The results showed that paeonol
(150 mg/kg and 300 mg/kg) effectively reduced the weight of EMT6 breast tumor. Compared with the control
group, paeonol significantly increased the number of tumor cells in G0/G1 phase, increased the number
of cells in apoptosis and decreased the number of cells in S phase...
Bhatia, Manish S.; Pakhare, Krishna D.; Choudhari, Prafulla B.; Kokare, Chandrakant R.
Fungi play a predominant role in microbial infections with serious health risk to immunocompromised individuals, including AIDS, cancer, diabetics, newborns and elderly patients. Fungi specific riboflavin metabolism involves lumazine synthase catalyzed synthesis of 6,7-dimethyl-8-D-ribityl lumazine which is converted to riboflavin by a riboflavin synthase. Therefore lumazine synthase has been targeted for design of newer antifungal agents. 32 novel N-substituted aryl amine derivatives have been designed, synthesized, characterized and screened as antifungals. Molecular modelling and docking studies with fungal lumazine synthase using the 32 inhibitors have elucidated unique binding areas within the active site of the enzyme. Amongst the selected 2-D...
Dias, Remeth J.; Mali, Kailas K.; Shinde, Jitendra V.; Havaldar, Vijay D.; Mali, Rahul K.
The prolonged residence of drug formulation in the nasal cavity is of utmost importance for intranasal drug delivery. The objective of the present investigation was to develop a mucoadhesive in situ gel with reduced nasal mucocilliary clearance in order to improve the bioavailability of the antiemetic drug, metoclopramide hydrochloride. The in situ gelation upon contact with nasal mucosa was conferred via the use of the thermogelling Pluronic flake 127 (18%). Mucoadhesion was modulated via the use of hydroxy propyl methyl cellulose (HPMC), sodium carboxy methyl cellulose (Na CMC) and sodium alginate (Na-alginate) whereas drug release was modified by varying concentrations...