Recursos de colección

Universidad Nacional de La Plata (75.269 recursos)

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vol. 29, no. 08

Mostrando recursos 1 - 20 de 32

  1. Validation of a spectrophotometric method in the visible region for the quantification of kojic acid in raw materials and products

    Piantavini, Mário S.; Stremel, Dile P.; Trindade, Ângela C.L.B.; Pontarolo, Roberto
    A spectrophotometric method in the visible region was developed and validated for the quantification of kojic acid (KA) in raw materials and products. According to the validation results, this method yields linearity in the range of 5 and 50 μg/mL of KA (r = 0.99998), selectivity, suitable sensitivity, precision (RSD 1.33 % and 1.21 % for intermediate precision and repeatability respectively), accuracy (recovery near 100 %) and robustness (varying pH, temperature and reading time) were measured and led to the validation of this method.These methods were tested and validated for various parameters according to ICH guidelines and USP.

  2. In vitro antioxidant potential of different solvent extracts of Naregamia alata

    Joseph, Jince M.; Sowndhararajan, Kandhasamy; Loganayaki, Nataraj; Manian, Sellamuthu
    Total phenolics, tannins, flavonoids and the antioxidative properties of the traditionally used medicinal plant Naregamia alata Wight. & Arn. were assessed. The hot water extract contained higher levels of total phenolics, tannins and flavonoids. The extracts were subjected to assess their potential antioxidant activities using various in vitro systems such as DPPH, ABTS•+, FRAP, β-carotene linoleic acid bleaching system, phosphomolybdenum reduction and Fe2+ chelation. It is concluded that N. alata may serve as a potential source of natural antioxidants capable of offering protection against free-radical mediated damages.

  3. Chemical composition and antifungal activity of the essential oil from Piper amalago L.

    Carrara, Vanessa da S.; Souza, Amanda de; Dias Filho, Benedito Prado; Nakamura, Celso Vataru; Paulo, Luís F. de; Young, María C. M.; Svidzinski, Terezinha I. E.; García Cortez, Diógenes Aparício
    The essential oil obtained from the leaves of Piper amalago L. by hydrodistillation was analyzed by CG-MS. The yield essential oil from fresh plant material was 0.1%. The main compounds found were β-copaen-4-α-ol (26 %), 7-epi-α-eudesmol (21.84 %), epi-α-cadinol (12.70 %), and n-hexyl-benzoate (12.29 %). The essential oil demonstrated antifungal activity against nine Candida strains, as shown using the agar-diffusion method.

  4. HPTLC method for quantitative determination of granisetron hydrochloride in bulk drug and in tablets

    Lakshmana Prabu, S.; Selvamani, P.; Latha, S.
    A new simple, rapid and reproducible high performance thin layer chromatographic method has been developed and validated for the analysis of granisetron hydrochloride (GSH) in bulk drug and from pharmaceutical formulation. The chromatographic separation was achieved on HPTLC aluminium plates precoated with silica gel 60F254 as the stationary phase with chloroform: methanol (80:20 %v/v) as mobile phase. The method gives a compact band for GSH (Rf value of 0.45 ± 0.02). Densitometric analysis of GSH was carried out in the absorbance mode at 301 nm. The linear regression analysis data for the calibration plots showed good linear relationship with the...

  5. Effects of single oral and topical administration of D-002 (beeswax alcohols) on xylene-induced ear edema in mice

    Ravelo, Yazmin; Molina, Vivian; Carbajal, Daisy; Arruzazabala, María de Lourdes; Más, Rosa; Oyarzábal, Ambar; Pérez, Yohani; Jiménez, Sonia
    D-002 (beeswax alcohols) contains triacontanol as most abundant component. Local application of triacontanol has been shown anti-inflammatory effects on chemically-induced dermatitis and oral treatment with D-002 produced anti-inflammatory effects in carrageenan-induced pleurisy and cotton granuloma in rats, but its effects on xylene-induced mouse ear edema had not been studied. This study investigated the effects of single oral and topical doses of D-002 on this model. Oral dosing groups were composed by one negative control and six xylene-treated, one positive control, four D-002-treated (25, 50, 200, 400 mg/kg), and one indomethacin-treated (10 mg/kg). Topical dosing groups were conformed by one negative...

  6. Dosage of atenolol tablets by spectrophotometry with phenol red

    Ramírez Maisuls, Hugo Enrique; Monzón, Celina M.; Delfino, Mario R.; Sarno, María del C.
    A spectrophotometric method is proposed for the analysis of atenolol, based on its reaction with the indicator phenol red in an acetone environment. Experimental conditions for the formation of an ion pair complex peaking at 388 nm were optimized. The method is linear in the range of 1 to 5 mg/mL with a correlation coefficient (n = 30) of 0.9968. Stoichiometry of the reaction is 1: 1. Free energy change (ΔG) for complex formation and stability constant (KF) have been calculated. Working with placebo it was found that excipients do not interfere in the analysis. The proposed method was applied...

  7. Synthesis and pharmacological study of some novel thiazolidinones

    Joshi, Vishal D.; Bhat, Ishwar K.; Jayaraman, R.; Dahake, Akash P.; Desai, Arvind R.
    A series of some novel thiazolidinones derivatives were synthesized and evaluated for their pharmacological activities. Thiazolidinones were synthesized from p-nitro aniline in four steps. First Schiff’s bases (V1- 8) were prepared by reacting the N4 -(4-nitrophenyl) thiazole-2,4-diamine (3) of p-nitro aniline derivatives with different aromatic aldehydes. Cyclocondensation of the Schiff’s bases with thioglycolic acid in presence of anhydrous zinc chloride resulted in the formation of the corresponding thiazolidinone (VD1-8) analogues. The structures of the newly synthesized compounds have been established on the basis of their spectral data. The synthesized selected compounds were evaluated for their anti-inflammatory and analgesic activity.

  8. Antinociceptive and anti-inflammatory activity of ferula hermonis root oil in experimental animals

    Geroushi, Afaf; Auzi, Abdurazag A.; Elhwuegi, Abdalla S.; Elzawam, Fawzi; El-Sherif, Akram; Nahar, Lutfun; Sarker, Satyajit D.
    Ferula hermonis (Apiaceae) is a well known Middle-Eastern medicinal plant. It has long been used traditionally as an aphrodisiac agent. The antinociceptive and anti-inflammatory activities of the root oil of F. hermonis were evaluated by the hot-plate test, the acetic acid-induced writhing test and the carragennan-induced rat paw edema test. In the hot-plate test, the root oil in oral doses of 400 and 800 mg/kg significantly increased the reaction time of animals to thermal pain, and in the acetic acid-induced writhing test, in similar oral doses it showed a considerable inhibition of acetic acid-induced writhing in mice in a dose-dependant...

  9. Toxicity study of a phytotherapic with Anemopaegma mirandum, Cola nitida, Passiflora alata, Paullinia cupana, Ptychopetalum olacoides and thiamin in rabbits

    Mello, Joao Roberto Braga de; Mello, Fernanda B. de; Langeloh, Augusto
    The phytotherapic formulation constituted by Anemopaegma mirandum (catuaba), Cola nitida (nóz de cola), Passiflora alata (maracujá), Paullinia cupana (guaraná), Ptychopetalum olacoides (marapuama) and thiamin chlorhydrate (Nerviton®) was investigated from the potential of toxicological effects when orally administered for 30 days to male and female New Zealand rabbits. The daily oral dose was ten times the prescribed dosage to humans. The general signs of toxicity, locomotion, behavior, respiratory rate and rhythm were evaluated. Body weight, food and water intake, rectal temperature, hematological and biochemical blood analysis, urinalysis, anatomopathological evaluation and visceral weight were measured. The results interpreted as a whole revealed...

  10. Self-medication, substance abuse and alcohol consumption in students attending to La Plata National University, Argentina

    Marín, Gustavo Horacio; Cañás, Martín; Carlson, Soledad; Silvestrini, M. P.; Corva, Santiago; Mestorino, Olga Nora; Errecalde, Jorge Oscar
    The World Health Organization raises self-care as a strategy for health promotion. Although self-medication is included in self-care strategy, often it is associate to an irrational medicine's usage. This factor associated with illicit drugs and alcohol consumption becomes in a public health problem. In order to establish frequency of self-medication and the prevalence of illicit drugs and alcohol consumption among university students of La Plata, Argentina, was started the present project. Information was collected by anonymous survey, that evaluated age, sex, origin, cohabitants, type, frequency of consumption, and career. A total of 5170 students were polled. 46.64 % consumes regularly...

  11. Effect of simvastatin on the generation of autoantibodies against oxidized LDL and progression of atherosclerosis in rabbits

    Cavallini, Daniela C. U.; Abdalla, Dulcinéia S. P.; Pauly-Silveira, Nadiége D.; Rossi, Elizeu A.
    Oxidized Low-Density Lipoproteins (oxLDL) and autoantibodies against oxLDL are important in the development of atherosclerotic lesions. Statins are efficacious in the control of dyslipidemia and prevention of atherosclerosis; however, many questions concerning the mechanism of action of such drugs remain unknown. This work investigated the effect of simvastatin on generation of autoantibodies against oxLDL and development of atherosclerosis in rabbits. The animals were divided into three groups: control, hypercholesterolemic, and hypercholesterolemic simvastatin (3.0 mg simvastatin/ kg body weight). Concentrations of autoantibodies against oxLDL were determined on days 0, 30 and 60 of the experiment and the atherosclerotic lesions were evaluated at the end of the study. Simvastatin...

  12. In vitro absorption studies of acyclovir using natural permeation enhancers

    Dias, Remeth J.; Mali, Kailas K.; Ghorpade, Vishwajeet S.; Garje, Sandeep B.; Havaldar, Vijay D.
    Gastroretentive Delivery Systems are employed to improve the bioavailability of drugs which are absorbed through upper part of GIT, by increasing their retention time. Incorporation of permeability enhancers in the formulations of such drugs can further increase their bioavailability; however their use in the formulations is questionable due to the toxicity exhibited by them. Acyclovir is a class III drug having low oral bioavailability due to improper absorption. Mucoadhesive tablets of acyclovir containing natural permeation enhancers were prepared by direct compression and evaluated for mucoadhesion strength, in-vitro dissolution parameters and in-vitro absorption studies. The formulations containing Aloe vera extract showed increase in the mucoadhesion strength and retarded...

  13. Formulation and gastrointestinal transit evaluation of mucoadhesive oral Multiple Unit Systems of Furazolidone

    Asane, Govind S.; Yamsani, Madhusudan R.; Bhatt, Jaykrishna H.; Kolhe, Mahesh H.; Mukkanti, Khagga
    The objective of present study was to improve gastric residence time of furazolidone by preparing mucoadhesive Multiple Unit Systems (MUS) with chitosan, Hydroxypropyl methyl cellulose K4M and sodium carboxymethyl cellulose by employing ionotropic gelation method. The resultant MUS were evaluated in vitro and in vivo. The particle size length ranged between 0.76 ± 0.25 to 0.89 ± 0.23mm, while the breadth was 0.76 ± 0.15 to 0.89 ± 0.06 mm, respectively. Encapsulation efficiency was in range of 82 to 90 %. MUS exhibited good mucoadhesive property in in vitro wash-off test. Stability studies showed no significant change in dissolution profiles...

  14. Total alkaloids from Solanum Iyratum Thunb. inhibited hela cells proliferation through induction of apoptosis and cell cycle arrest

    Lu, Wenzong; Ni, Yuan; Zhao, Chen; Zhang, Liang; Ren, Yumiao
    The object of the present study was to investigate the anticancer properties of total alkaloids from Solanum lyratum Thunb (SLT-A), including the inhibitory effect of SLT-A on HeLa cells and the apoptosis-inducing capacity in vitro. In our study, cytotoxicity was measured by the growth inhibition assay and detection of apoptosis was performed by Hoechst33324 and Tdt-mediated dUTP nick end labeling (TUNEL) staining assays. The in vitro cytotoxic studies were complemented by the cell cycle analysis and determination caspase-3 activity. Reverse transcription-polymerase chain reaction (RT-PCR) assay was applied on the expression of apoptosis-associated genes. The result showed that treatment of HeLa cells with SLT-A resulted in the growth...

  15. Anti-inflammatory effects of Fritillaria ussuriensis maxim

    Huang, Li-jing; Gao, Wenyuan; Li, Xia; Man, Shuli; Zhang, Yanjun; Huang, Luqi; Liu, Changxiao
    Bulbs of Fritillaria ussuriensis Maxim., usually known as Bulbus Fritillariae ussuriensis, (BFU) has been used as antitussive, antiasthmatic and expectorant in traditional herbal medicine. In this study, the aqueous extract of BFU (BFUE) was evaluated for its anti-inflammatory activity. Meanwhile, the content of PGE2 and MDA in inflammatory exudates was measured to explore the anti-inflammatory mechanisms of BFUE. In order to identify the active components of BFU, the total alkaloids (TA), the total flavonoids (TF) and the total saponins (TS) were evaluated for their bioactivities. Results showed that BFUE inhibited carrageenin-induced paw edema, xylene-induced auricular edema and acetic acid-induced vascular...

  16. Determination of atorvastatin and gemfibrozil in human plasma by reversed-phase liquid chromatography

    Khan, Islam U.; Ashfaq, Muhammad
    A simple, sensitive and reproducible RP-HPLC method was developed and validated for the simultaneous determination of atorvastatin and gemfibrozil in human plasma. After a single step liquidliquid extraction of both the drugs with acetonitrile, the separation was accomplished on a Merck C 18 column (250 x 4.6, 5 µm). Diode array detection at a wavelength of 240 nm was carried out with a mobile phase comprising of a mixture of 0.1M ammonium acetate buffer (pH 5.0) and acetonitrile in the ratio of (45:55, v/v). The method was linear in the concentration range of 0.1-20 µg/ml for atorvastatin and 6-1200 µg/ml...

  17. Injectable thermosensitive gels based in poloxamer as modified drug release systems for veterinary use

    Bermúdez, José M.; Grau, Ricardo
    The purpose of this work is to explore the potential of combining poloxamer 407 and carrageenan for its utilization in an injectable depot drug release system. Reverse thermal gelation of these formulations allow the local injection in liquid form, gelling in situ after its administration. Carrageenan reinforces the structure of poloxamer gels (after 50 h of testing only 20 % of the system is eroding) and allows to modulate the release rate of progesterone as a function of formulations composition. The elastic modulus of sole poloxamer gels (G' = 56 Pa) increases significantly in presence of carrageenan (G' = 1...

  18. Anti-inflammatory effect of diclofenac diethylammonium gel on acute phase of ligature induced periodontitis in rats

    Botelho, Marco A.; Paixão, Mônica S.; Rachid, Ítalo; Bannet, Leonard Edward; Patrus, Ana Helena; Mattos, Thiago Borges; Queiroz, Dinalva; Ruela, Ronaldo; Costa, Jose M. C; Quintans Júnior, Lucindo José
    This study aimed to evaluate the effect of a diclofenac diethylammonium gel 10 mg/g (DD) on acute phase of ligature induced periodontitis model in rats. Experimental Periodontitis Disease (EPD) was induced in 30 Wistar rats subjected to ligature placement on left molars. Animals were treated with (DD), immediately after (EPD) induction. Saline-based gel (SG) was utilized as negative control and DD gel 10 mg/g was the tested substance. Animals were randomly assigned into the groups. The periodontium and the surrounding gingiva were examined at histopathology, as well as the neutrophil influx into the gingiva was assayed using myeloperoxidase activity levels...

  19. Novel vesicular approach for topical delivery of baclofen via niosomes

    Keservani, Raj K.; Sharma, Anil K.; Ramteke, Suman
    Niosomes have been reported as a possible approach to improve the low skin penetration and bioavailability characteristics shown by conventional topical vehicle for Baclofen (centrally acting skeletal muscle relaxant). Niosomes were prepared by lipid film hydration method using non ionic surfactant (Span 20) and cholesterol in varying molar ratios such as 1:1, 1:2 and 2:1. The prepared systems were characterized for vesicle surface morphology, entrapment efficiency, Osmotic fragility and stability studies. The in vitro drug release behavior was determined by an in-house fabricated dissolution-dialysis apparatus. The skeletal muscle relaxant activity was determined by rota rod method using Swiss albino mice. The average particle size of niosomes was...

  20. Physicochemical quality evaluation of acetylsalicylic acid tablets distributed by a basic pharmacy

    Bunhak, Élcio; Stoef, Paulo R.; Melo, Eduardo B. de
    The present paper aims to investigate the physicochemical quality of pharmaceutical drugs acquired and distributed by the Basic Pharmacy of the city of Cascavel-Paraná State. During the period between June 2004 and March 2006, samples of eight batches of acetylsalicylic acid 100 mg produced by two laboratories were analyzed. Following the techniques and specifications established by official compendiums, physicochemical tests of disintegration, dissolution, hardness, friability, uniformity of mass, content and limit of free acetylsalicylic acid were carried out. All the analyzed products presented quality deviations. All the six batches produced by one of the laboratories failed in the punctual dissolution...

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