Recursos de colección

Universidad Nacional de La Plata (74.538 recursos)

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vol. 30, no. 05

Mostrando recursos 1 - 20 de 34

  1. Didanosine-loaded chitosan microspheres: optimization of fabrication process

    Silva, Classius F. da; Severino, Patrícia; Martins, Fernanda; Santana, Maria H. A.
    Didanosine (ddI) is an important drug in the AIDS therapy. However, to overcome the instability of ddI in acid medium, the tablets have a large amount of buffer that can lead to side effects such as diarrhea and kidney problems. Microencapsulation of ddI can reduce the excessive use of buffers. In this work we used the ionotropic gelation technique in the preparation of chitosan microspheres. The microspheres were characterized according to the incorporation efficiency, loading capacity, degree of swelling, permeability and morphology. The best results were obtained for 10 g of chitosan/g of sodium tripolyphosphate. Subsequently, changes were carried out...

  2. Evaluation of physical properties of chitosan active membrane loaded with phytotherapic extract and silver sulfadiazine

    Camargo, Mariana; Mahl, Cynthia R.A.; Besinela Jr, Expedito; Silva, Classius F. da
    Polymeric membranes have been used as wound dressings for burns and healing wounds. Healing properties, anti-inflammatory and antibiotic in such membranes can be induced and/or made possible with the incorporation of bioactive as phytotherapic extract. In this work chitosan membranes were produced incorporating a commercial phytotherapic extract. The membranes were prepared by casting a chitosan solution and phytotherapic extract in a Petri dish and dried for 24 hs. We studied the effect of the concentration of the phytotherapic extract (PE) on membranes properties. Additionally, membranes were prepared with silver sulfadiazine (SS), an antibiotic ointment used for burns. The membranes were...

  3. Comparative pharmacological evaluation of Hypericum perforatum and Rosmarinus officinalis for anti-depressant activity

    Devi, Kshama; Satish, Hanumantharayashetty; Khajuria, Deepak Kumar; Moinuddin, Ghulam
    The comparative antidepressant activity of the extracts of Hypericum perforatum and Rosmarinus officinalis using animal models of depression were evaluated. The herbal extracts of Hypericum perforatum (20 mg/kg/p.o.), Rosmarinus officinalis (500 mg/kg/p.o.) were subjected for its antidepressant activity using four different animal models of depression, viz: Forced Swimming Test (FST), Reserpine Reversal Test (RRT), Haloperidol-Induced Catalepsy (HIC), and Pentobarbitone Sleeping Time (PST) in male Wistar rats. The study after single oral administration of Hypericum perforatum and Rosmarinus officinalis extracts revealed a statistically significant reduction in immobility time in FST, RRT, and protection against HIC, compared to control respectively. However, there...

  4. Anti-staphylococcus activity of Ibicella lutea compounds

    Wallace, Federico; Vázquez, Álvaro
    Ibicella lutea (Lindl.) Van Eselt. (Martyniaceae) is the sole species of the genus Ibicella from the small Martyniaceae family comprising just 3 genera (Martynia, Proboscidea and Ibicella) with 13 species. In Uruguay, where the plant grows freely, it is called “cuerno del diablo” (Devil’s horn) and its infusion is used in popular medicine as an antimicrobial for the treatment of eyes and skin infections. In this work we present the antibacterial activity of two compounds isolated from I. lutea against several S. aureus strains, both sensible and resistant. Both compounds were active against at least some of the S. aureus...

  5. In vitro sun protection factor evaluation of sunscreens containing rutin and its derivative

    Pedriali, Carla A.; Iizuka, Samara S.; Balogh, Tatiana S.; Pinto, Claudinéia A. S. O.; Kaneko, Telma Mary; Baby, André R.; Velasco, María Valéria R.
    This research aimed at determining spectrophotometrically (290 to 320nm) the in vitro Sun Protection Factor (SPF) of sunscreens developed with rutin (R) or succinate rutin (SR), in association or not with UVB filter. Formulations were developed based on phosphate-base O/W emulsions, with (B) or not (A) the presence of polyacrylamide/C13-14 isoparaffin/laureth-7 (PIL), in accordance with the following associations: (a) control; (b) 1.0 % SR; (c) 0.1 % R; (d) 7.5 % ethylhexyl methoxycinnamate (EHMC); (e) 7.5 % EHMC + 0.1 % RS; (f) 7.5 % EHMC + 0.1 % R. It was verified a statistical significative elevation of the SPF...

  6. Pharmacokinetics of lansoprazole injection in peptic ulcer and healthy volunteers

    Yang, Dongzhi; Gao, Yuanyuan; Jiang, Xianglan; Dong, Weifeng; Tang, Daoquan
    The pharmacokinetics of lansoprazole after a single intravenous dose of 30 mg was determined in 10 healthy volunteers and 10 peptic ulcers patients. In this work, a liquid-liquid extraction and enrichment method with RP-HPLC determination route was taken with high sensitivity and low limit detection of 5 ng/mL. The concentration-time curves in the two groups were best fitted to a two-compartment model, but their main kinetic parameters were remarkably different between healthy and ulcers volunteers. The mean maximum plasma concentration (Cmax ) and area under the curve (AUC0t ) were increased from 975.8 ng/mL to 1298.7 ng/mL and from 1439...

  7. Fabrication of novel gastro-retentive Floating microcapsules by utilizing a combination of HPMC and EC and their physicochemical assessment

    Aamir, Muhammad N.; Ahmad, Mahmood; Khan, Shujaat A.; Akhtar, Naveed; Murtaza, Ghulam; Akhtar, Muhammad; Rasool, Fatima
    The project was aimed to develop floating microcapsules (FMs) using combination of a hydrophobic (ethyl cellulose, EC) and hydrophilic polymer (hydroxy propyl methyl cellulose, HPMC), and to evaluate them. An easy and novel phase separation method was adopted to prepare FMs. Chloroform and paraffin oil were employed as solvent and non-solvent respectively. Five kinetic models were applied to assess and describe the mechanism and pattern of tramadol hydrochloride (TmH) release from FMs and they were subjected to FTIR and XRD to evaluate TmH-HPMC-EC interaction. As EC concentration was increased the retardation in drug release, improvement in flow characteristics and decrease...

  8. Effect of ashwagandha and aloe vera pretreatment on intestinal transport of buspirone across rat intestine

    Yamsani, Shravan K.; Devandla, Adukondalu; Yamsani, Vamshi V.; Athukuri, Bhargavilatha; Gannu, Ramesh; Palem, Chinna R.; Rao , Yamsani Madhusudan; Manda, Sarangapani
    The transport of buspirone across rat intestine (duodenum, jejunum, ileum and colon) was studied by using the non-everted sac method. Rats were pretreated with ashwagandha (Withania somnifera) and Aloe vera juice for 7 days. The rats were sacrificed by using anesthetic ether, the intestinal segments were isolated and used for the studies. The probe drug (buspirone) solution was placed in the isolated intestinal sac. Samples were collected at preset time points and replaced with fresh buffer. The drug content in the samples was estimated using high performance liquid chromatography method. Control experiments were also performed. The results reveal that there...

  9. Diclofenac quantification: analytical attributes of a spectrophotometric method

    Monzón, Celina M.; Delfino, Mario R.; Sarno, María del C.; Delfino, Mario R. (h)
    An spectrophotometric UV-visible technique used to quantify diclofenac and its application to pharmaceutical preparations is described, based on diclofenac oxidation by Fe(III) in the presence of ophenanthroline. The formation of tris (o-phenanthroline)-Fe(II) complex (ferroin) upon diclofenac reaction was investigated. Absorbance of ferroin complex was measured at 506 nm. This method was tested on 50 mg tablets. Operating with placebos, it was found that excipients do not interfere with the determination. A good linearity was found [y = (0.0294 ± 0.0041)x + (0.1326 ± 0.0559)] with r2 = 0.9982, calibration curve showed a linear range from 5-15 μg/mL of diclofenac. The...

  10. Extemporaneous oral preparations: an alternative for hospitalized children

    Santos, Luciana dos; Heineck, Isabela
    The lack of pharmaceutical formulations proper for children favors the manipulation of extemporaneous oral preparations by hospital pharmacies. The study aimed to identify the medications reformulated by the pharmacy of Hospital de Clínicas de Porto Alegre, as well as the availability for use in oral liquid formulations on Pediatrics. The drugs were classified according to the criteria approved by the Food and Drug Administration; for that, the DrugDex-Micromedex® database was used, while the reformulated items were classified as unlicensed. 2026 medicines in the 342 prescriptions were analyzed, while 35 % were classified as off-label use and 14.9 % as not...

  11. Application of UPLC to separation and analysis of ginsenosides from cultivated ginseng and forest-grown wild ginseng

    Zhang, Lanlan; Gao, Wenyuan; Zhou, Shuiping; Cai, Nan
    In the past years, much attention has been paid to Panax ginseng, mainly referring to Cultivated Ginseng (CG), but few studies have also been focused on Forest-grown Wild Ginseng (FWG). FWG has been included in the 2010 edition Pharmacopoeia of The Peoples Republic of China, as a substitute for wild ginseng. This paper compared and determined the variety and changes of ginsenosides in CG and FWG by UPLC. The results demonstrated that the content of every ginsenoside was the highest at tenyear-old FWG, but the lowest at fourteen-year-old FWG. Pharmacological activity varies for different ginsenosides, therefore, this study will provide...

  12. Determination of salmeterol xinafoate and its degradation products according to ICH guidelines with use of UPLC technique

    Patel, Pratibha; Sahu, Kapendra; Karthikeyan, Chandrabose; Moorthy, N.S.H.N.; Trivedi, Piyush
    The objective of reducing analysis time and maintaining good efficiency, there has been substantial focus on high-speed chromatographic separations. Recently, commercially available ultra performance liquid chromatography (UPLC) has proven to be one of the most promising developments in the area of fast chromatographic separations. In this work, a new isocratic reverse phase chromatographic stability indicating assay method was developed using UPLC for salmeterol xinafoate bulk drug. A novel stability-indicating UPLC assay method was developed and validated for salmeterol xinafoate and its degradation products. An isocratic UPLC method was developed to separate the drug from the degradation products, using an Acquity...

  13. Synthesis and biological evaluation of benzylideneamino-5H-thiochromeno[2,3-b]pyridin-5-one

    Khan, Muhammad N.; Khan, Misbahul A.; Munawar, Munawar A.
    Various benzylideneamino-5H-thiochromeno[2,3-b]pyridin-5-one derivatives were synthesized by the reaction of 7-amino-5H-[1] benzothiopyrano[2,3-b]pyridin-5-one and different benzaldehydes. Their structures were confirmed on the basis of Mass, 1H NMR, IR spectra and elemental analysis. These compounds were screened for their antioxidant and antibacterial properties. The results reveal that some compounds have pronounced antioxidant and antibacterial activities.

  14. In vitro and in vivo release studies of fluorouracil acetic acid-dextran conjugates

    Hao, Aijun; Deng, Yingjie; Chen, Changlan; Zheng, Ying; Chen, Lijiang
    Fluorouracil acetic acid–dextran (FUD) conjugates were synthesized and its stability in buffer solution has been investigated previously in our laboratory. In this contribution, the in vitro and in vivo releases of FUD were investigated. The results revealed that no detectable 5-fluorouracil (5-FU, FU) found during in vitro and in vivo release studies. The in vitro release was dependent on both degree of substitution (DS) of 5-fluorouracil-1-acetic acid (5-FUA) in FUD and gastrointestinal tract section (GITs). 5- FUA can be completely released from the conjugates with DS of 10.3 % (wt/wt) in homogenates of cecum and colon content. After oral administration...

  15. Release behavior and bioadhesivity of a hydrophobic polyacid gel

    Cornejo-Bravo, José M.; Ganem-Quintanar, Adriana; Piñón-Segundo, Elizabeth; Serrano-Medina, Aracely; Quintanar-Guerrero, David
    The release behavior of salicylic acid and the bioadhesion properties of crosslinked polyacid hydrophobic-gel disks, containing methyl methacrylate-co-methacrylic acid, were evaluated in simulated fluids. In gastric fluid a very low amount of drug was released with a minimal swelling and water uptake. The drug release was dependent of the drug precipitated in the interconnecting micropores of the disk structure. The transport and release type in this medium ranged from Fickian to zero order depending of drug loading. In intestinal fluid, disks swelled completely and the water absorption capacity was eight-fold its dry weight. In this case the released drug showed...

  16. Formulation and characterization of rifabutin loaded floating gellan gum beads

    Verma, Anurag; Pandit, Jayant K.
    Rifabutin loaded floating gellan gum beads were prepared by Ca++ induced ionotropic gelation in acidic medium by drop wise addition of gellan gum dispersion containing drug and gas-generating agent. The prepared beads were evaluated for in vitro characterization and in vivo Helicobacter pylori clearance efficiency following repeated oral administration to H. pylori infected albino rats. Live cell staining of stomach homogenates of H. pylori infected animals treated with rifabutin showed pronounced anti H. pylori activity. Our results suggested that the floating gellan gum beads may be used for a potential oral stomach-specific release system to treat stomach-specific infections like multi-drug...

  17. Comparative in vitro transmucosal permeation of three proprietary diclofenac gels

    Tripathi, Poonam; Pandey, Awanish; Pandey, Rishabh D.; Pandey, Aditya
    Studies to evaluate the clinical efficacy and safety of various topical NSAIDS compared to oral NSAIDS have been reported but little research has been conducted to evaluate the efficacy of topical NSAIDS from different manufacturers containing the same concentation of medicament. The aim of this study was to make comparison of the in vitro release rate of three proprietary Diclofenac gels. To study the in vitro potential of these formulation permeation studies were performed with Franz diffusion cell by using cellulose acetate membrane. In vitro permeation study was found that the diffusion profile of A gel was equivalent to Voveran...

  18. Alginate/hydrophobic HPMC (60L) particulate systems: new matrix for controlled release of diclofenac potassium

    Ray, Sarbani D.; Ghosal, Kajal; Ghosal, Ingana; Ghosh, Dipanwita
    This work is focused on the development of a new particulate drug delivery system using sodium alginate (SA) and modified hydrophobic hydroxyl propyl methyl cellulose (HPMC, 60L grade) containing diclofenac as a model drug molecule for intestinal drug prolonged release. Diclofenac loaded HPMC-SA beads have been developed by ion exchange methods and were characterized by encapsulation efficiency, drug release profile, swelling and matrix erosion rate. Matrix swelling of calcium alginate beads induced by phosphate buffer ends up in erosion and destruction. However, for HPMC-SA beads do not lead to complete erosion, which may be the main cause of diclofenac retention...

  19. Characterization and basic guidelines for the quality control of chitosan for drug delivery in pharmaceutical applications

    Cunha Júnior, Armando S. da; Moura, Edson S.; Heneine, Luiz G. D.; Faraco, André A.G.
    Chitosan is increasingly used as an alternative to synthetic polymers due to its water solubility, biodegradability, and biological activity. In this work, chitosan samples from three suppliers were evaluated and characterized by physicochemical methods in an attempt to analyze pharmaceutical applications. The degree of deacetylation (DD) was determined by 1H NMR, IR spectra, potentiometric titration, and UV spectrophotometry. The analysis was supplemented by the techniques of DSC, TG, loss on drying, pH of the suspension, heavy metals, sulphated ash, and triple detector size exclusion chromatography (TriSEC). The use of UV, IR, and 1H NMR techniques to determine the degree of...

  20. Development of novel microemulsion-based hydrogel for topical delivery of sinomenium

    Gui, Shuangyin; Pan, Enyuan; Liu, Peng; Lu, Chuanhua; Peng, Daiyin
    The objective of the present investigation was to develop and evaluate microemulsion-based hydrogel (MBH) for the topical delivery of sinomenium. The solubility of sinomenium in oils and surfactants was evaluated to identify components of the microemulsion, the pseudo-ternary phase diagrams were developed to identify the area of microemulsion existence and obtain the optimization Km (the weight ratio of surfactant to cosurfactant). The transdermal ability of various microemulsion formulations were evaluated in vitro using Franz diffusion cells fitted with rat skins and sinomenium was analyzed by HPLC. The permeation of microemulsions accorded with the Fick’s first diffusion law and the optimal...

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