Recursos de colección

Universidad Nacional de La Plata (75.687 recursos)

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vol. 30, no. 10

Mostrando recursos 1 - 20 de 32

  1. Determination of ramosetron in rat plasma by LC-ESI-MS and its application

    Lin, Guanyang; Chen, Xiaole; Zheng, Meiqin; Wang, Youpei; Pang, Lingxia; Wang, Qing
    A sensitive and simple liquid chromatography/electrospray mass spectrometry (LC-ESI-MS) method for determination of ramosetron in rat plasma using one-step protein precipitation was developed and validated. After addition of midazolam as internal standard (IS), protein precipitation by acetonitrile was used as sample preparation. Chromatographically separation was achieved on an SB-C18 (2.1 mm × 150 mm, 5 μm) column with acetonitrile-0.1 % formic acid as the mobile phase with gradient elution. Electrospray ionization (ESI) source was applied and operated in positive ion mode; selected ion monitoring (SIM) mode was used to quantification using target fragment ions m/z 280 for ramosetron and m/z...

  2. Microscopic diagnosis of the leaf and stem of Lucilia nitens Less., Asteraceae

    Menarim, Daniele O.; Matzenbacher, Nelson I.; Budel, Jane M.; Duarte, Márcia do Rocio
    Lucilia nitens is a herb used in folk medicine for treating diarrhoea and popularly called in Brazil "erva-pombinha". Aiming to expand the knowledge on this medicinal plant, this work has dealt with anatomical analysis of the leaf and stem for microscopic diagnosis. Standard procedures of light and scanning electron microscopy were carried out. The leaf is amphistomatous and has abundant non-glandular trichomes and few glandular ones. The mesophyll is isobilateral and the midrib, in transection, is planoconvex. It is traversed by a collateral vascular bundle presenting sclerenchymatic caps. The stem has onelayered epidermis, lacunar collenchyma and parenchymatic pith. Among the...

  3. Determination of ibudilast in rabbit plasma by liquid chromatography-mass spectrometry and its application

    Hu, Lufeng; Chen, Xiaole; Ma, Jianshe; Wang, Xianqin; Sun, Wei; Dong, Wei; Yang, Fangfang; Ding, Chen
    A sensitive and selective liquid chromatography-mass spectrometry (LC-MS) method for determination of ibudilast in rabbit plasma was developed and validated. After addition of estazolam as internal standard (IS), protein precipitation by acetonitrile was used as sample preparation, and chromatography involved Agilent SB-C18 column (2.1 mm x 50 mm, 3.5 μm) using 0.1 % formic acid in water and acetonitrile as a mobile phase with gradient elution. Detection involved positive ion mode electrospray ionization (ESI), and selective ion monitoring (SIM) mode was used for quantification of target fragment ions m/z 230.7 for ibudilast and m/z 294.7 for estazolam (internal standard, IS)....

  4. Vitamin D deficiency and acute respiratory infections among brazilian children

    Barberato Fihlo, Silvio; Lopes, Luciane C.; Zabotto, Cláudia B.; Gerenutti, Marli; Del Fiol, Fernando S.
    The prescription of antibiotics worldwide remains large, especially in children. The lack of accurate diagnoses leads to overuse of these drugs, increasing bacterial resistance. A large number of studies have shown that another important factor in the onset of upper respiratory infections (URI) is inadequate intake of nutrients for these children, especially vitamins A, C and D. This study aimed to understand the use of antibiotics and the profile of nutrient intake in children diagnosed with URI. During 12 months, a survey (24 h dietary recall) was applied in 131 parents of children using antibiotics. The results showed antibiotic prescriptions...

  5. Enhancement of dissolution profile of gliclazide by solid dispersion adsorbates

    Singh, Narender; Arora, Ashish; Kaushik, Deepak
    This article investigates enhancement of the dissolution profile of gliclazide, an antidiabetic drug, using the combination of solid dispersions and melt adsorption techniques. Poloxamer and PEG 6000 were utilized as hydrophilic carriers for solid dispersions preparation and lactose selected on the basis of preliminary studies was utilized as an adsorbent for the preparation of solid dispersion adsorbates. The techniques of FTIR spectroscopy, differential scanning calorimetry (DSC), and X-ray diffractometry (XRD) were performed to characterize the solid dispersions and to identify the physicochemical interaction between drug and carriers. Dissolution rate of gliclazide was higher in case of solid dispersion adsorbates as...

  6. Multiple compounds determination and fingerprint analysis of herbal preparation Shuang-Huang-Lian capsule by HPLC-DAD

    Zhao, Ziming; Jiang, Xianglan; Yin, Xiaoxin; Tang, Daoquan; An, Yi-qiang; Chen, Xu; Wu, Jing
    The objective of this paper was to develop a high performance liquid chromatography with diode array detection both for chromatographic fingerprint and simultaneous determination of twelve analytes of Shuang-Huang-Lian (SHL) capsule. The chromatographic separation was performed on an Aglient Zorbax SB-C18 column with a gradient elution program using a mixture of acetonitrile and 0.2 % acetic acid as mobile phase within 110 min detected at 278 nm wavelength. For fingerprint analysis, 50 peaks were selected as the common peaks to evaluate the similarities of different samples collected from different pharmaceutical companies in China, and two kinds of data, relative retention...

  7. Fast determination of voriconazole in oral fluid using microextractionby packed sorbent and HPLC with fluorescence detection

    Antunes, Marina V.; Linden, Rafael; Pasqualotto, Alessandro C.; Andreolla, Huander F.; Spaniol, Bárbara; Bordin, Natália A.
    A fast and reliable method for the determination of voriconazole in oral fluid using microextraction by packed sorbent and liquid chromatography with fluorescence detection was developed and validated. MEPS was performed at basic pH with only 50 μL of oral fluid and the extract was injected without an evaporation step. The overall procedure, including extraction and chromatographic analysis, took only 15 min. Voriconazole and internal standard were separated on a Lichrospher RP 8ec column (250 x 4 mm, particle diameter 5 μm) eluted with a mobile phase composed of phosphate pH 2.3 (containing 0.1 % triethylamine) and acetonitrile (64:36, v/v)...

  8. Incidence of adverse drug reactions at a university hospital in Southern Brazil

    Gonzalez, Cristina; Tust, Patrícia B.
    We aimed to evaluate the incidence of adverse drug reactions (ADRs) at a hospital, determine the variation in the incidence of ADRs between genders and among age groups, and determine the pharmacological class most often involved in ADRs. A longitudinal prospective study was conducted over a period of 5 months by using an active search in all hospital units. Four hundred forty-six patients were followed during their hospitalization; 103 showed ADRs, resulting in a 23 % overall incidence. Suspected ADRs were identified in 136 patients. From this group of patients, 9 reactions were classified as definite (6.6 %), 77 as...

  9. Effect of total flavonoids from Chrysanthemum indicum on ultrastructure and secretory function of synoviocytes in adjuvant arthritis rats

    Hu, Rong; Jiang, Hui; Zhang, Lei; Cheng, Wen-Ming; Li, Jun; Chen, Xiao-Yu
    Chrysanthemum is a traditional Chinese medicine used in China to treated inflammatory disease. The total flavonoids of Chrysanthemum indicum (TFC) were extracted from the dried bud of C. indicum. Our previous study had demonstrated TFC was a new class of effective anti- inflammation, analgesia and immunoloregulation agents. In this study, we established an adjuvant arthritis (AA) model by injection of Freund’s Complete Adjuvant (FCA) to investigate the effect of TFC on chronic autoimmune disease. Secondary paw swelling of AA rats was measured with volume meter and polyarthritis index was scored. IL-1β and TNF-α production in synoviocytes were determined by radioimmunoassay....

  10. Thermodynamics of sulfanilamide solubility in propylene glycol + water mixtures

    Martínez, Fleming; Romdhani, Asma; Delgado, Daniel R.
    The solubility of sulfanilamide (SA) in propylene glycol + water cosolvent mixtures was determined at temperatures from 293.15 to 313.15 K. The thermodynamic functions: Gibbs energy, enthalpy, and entropy of solution and mixing were obtained from these solubility data by using the van’t Hoff and Gibbs equations. The solubility was maximal in propylene glycol and very low in water at all the temperatures. A non linear enthalpy–entropy relationship was observed from a plot of enthalpy vs. Gibbs energy of solution. The maximum point in plot of ΔsolnH° vs. ΔsolnnG° (0.20 mass fraction of propylene glycol) separates two different trends, one...

  11. New validated RP-UPLC method for determination of aripiprazole assay in aripiprazole tablets

    Negi, Bhawana; Bansal, Ruchi; Singh, Dhirendra P.; Singh, Lokesh K.
    A simple, economic and time-efficient, isocratic reverse-phase ultra performance liquid chromatographic (RP-UPLC) method has been developed to analyze aripiprazole in tablets. Successful chromatographic elution and quantification of the drug was achieved on a Waters Symmetry C18, 100 mm x 4.6mm, 3.5 μm column, UV detection at 220 nm with a isocratic mobile phase comprising a mixture of component A (pH 2.5, phosphate buffer) and component B (methanol and acetonitrile (1:1, v/v) in the ratio of 45:55 (v/v). The flow rate was 1.0 mL/min. The method was validated for specificity, precision, linearity, accuracy, range, stability in analytical solution, robustness and system...

  12. Development and characterization of ternary solid dispersion systems of olmesartan medoxomil

    Rao, J. Venkateswara; Pore, Yogesh V.; Shinde, Vikram R.
    The ternary solid dispersion systems of poorly water soluble olmesartan medoxomil (OLM) were prepared by conventional kneading method in order to improve its physicochemical performance. A 32 full factorial design approach was employed to optimize influence of concentration of polyvinylpyrrolidone K30 (PVP) and poloxamer 407 (PLX) on physicochemical characteristics of these dispersions. All formulations were characterized by XRPD, DSC and dissolution studies. Physical studies revealed complete loss of crystallinity and formation of uniform molecular dispersion of OLM in its ternary systems. All dispersion systems showed significant improvement in dissolution profile in comparison to pure drug alone (p < 0.001). The...

  13. Effect of drug properties on formulation properties of eudragit non effervescent floating microparticulates

    Harikumar, S. L.; Singh, Satish
    The objective of the present investigation was to investigate the effects of selected drugs (captopril and celecoxib) properties on different parameters drug entrapment, in vitro drug release, release pattern, in vitro drug permeation and buoyancy in the formulation of Eudragit S100 non effervescent floating microparticulates. Microparticulates were in size ranges 268.36-352.27 μm (captopril) and 271.36- 365.54 μm (celecoxib). Encapsulation efficiency of celecoxib was good as compare to captopril. In vitro permeation studies showed in range (ES6) 74.83 μg – (ES1) 79.84 μg (celecoxib), (EU6) 57.01 μg - (EU1) 67.38 μg (captopril). In vitro release followed Non-Fickian diffusion mechanism while in...

  14. Hydrogel-thickened nanoemulsion with green coffee seed oil for topical delivery of vitamin A

    Santos, Orlando D. H.; Mezadri, Hygor; Aguiar, Tatiana A.; Oliveira, Juliana S.
    Nanoemulsions are special emulsions that consist in a very small drop with sizes between 20 to 500 nm. When they are intended for topical application, the main problem of nanoemulsions is their low viscosity, which is reflex of the small drop size. It was proposed the attainment of hydrogel-thickned nanoemulsions with vitamin A palmitate (retinyl palmitate) with green coffee seed oil by phase inversion. Hydrogel-thickned nanoemulsions with drops size ranging between 77 to 110 nm were obtained, depending on the polymer used, with increased viscosity. Rheological profile of developed hydrogel-thickned nanoemulsions was determined, showing the influence of the kind of...

  15. Bioequivalence evaluation of two different controlled release matrix formulations of ketoprofen tablets in healthy malaysian volunteers

    Mohammed, Kaleemullah; Fadli, Mohd; Yusuf, Eddy; Hamid, Junainah Abd; Chitneni, Mallikarjun; Elhassan, Gamal O.; Al-Dhalli, Samer; Wong, Jia W.; NG, Bee H.; Yuen, Kah H.; Khan, Jiyauddin
    The aim of this study was to evaluate the in vivo behavior of matrix tablets formulated with ketoprofen as a model drug after oral administrations in healthy Malaysian male volunteers and to compare its rate and extent of absorption with the commercially available tablet Apo-Keto SR® as a reference product. The test formulation containing 20 % HPC (GXF) as release retardant was selected in this regards. The bioequivalence study was conducted according to a single dose, randomized, 2-treatment, 2-sequence, 2-period crossover study design on six healthy non-smoking Malaysian adult male volunteers. Plasma concentrations of ketoprofen were determined by a high-performance...

  16. Potential drug therapy problems on an elderly nursing home population

    Dias Junior, Carlos A.C.; Ribeiro, Andréia; Soares, Adriana C.; Pereira, Mariana L.; Nascimento, Mariana M.G.
    The elderly population and the incidence of chronic diseases are growing rapidly in Brazil. This raises the demand for health services (like Nursing Homes - NH) and drugs, exposing this population to Potential Drug Therapy Problems (PDTP). A cross-sectional study in a Brazilian NH was developed through prescription analyses. PDTP were accounted when one of the following were detected: double therapy (DT); sub-dose; overdose; drug-drug interaction (DDI); food-drug interaction (FDI); Potentially Inappropriate Medication (PIM) according to the Beers Criteria; PIM according to the STOPP (Screening Tool for Older Persons’ Prescriptions). 116 PDTP were identified (17 DT, 16 sub-doses, an overdose,...

  17. Development and sedative effect of a new formulation of midazolam in chocolate bars

    Flores Pérez, Carmen; Juárez Olguín, Hugo; Flores Pérez, Janett; Ramírez Mendiola, Blanca; González Zamora, José F.; Alemón Medina, Radamés; Rivera Espinosa, Liliana; Chávez Pacheco, Juan L.
    The aim of this work was to assess the stability and sedative effect of midazolam in chocolate bars. The stability of 5 g chocolate bars containing 6 mg midazolam hydrochloride was evaluated at room temperature (25 ± 2 °C), at 4 and 40 °C, by HPLC. Drug plasma levels were measured and the sedative effect was confirmed in six healthy volunteers according to the Ramsay’s scale. Data regarding chocolate bar administration were compared to those from the apple juice solution. Pharmacokinetic data were processed using the WinNonLin 5.2 software. Midazolam in chocolate bars remained stable for 14 days at room...

  18. Preparation, evaluation and stability profile of alginate based floating multiparticulates of Simvastatin

    Barik, B. B.; Premchandani, T. A.
    The present study involves preparation and characterization of floating multiparticulate microcapsules with Simvastatin as model drug for prolongation of gastric residence time. The main objective is to improve solubility of simvastatin β-CD complex (1:2) by co-precipitation method and then to deliver the same in sustained release dosage form. Sustained-release simvastatin microcapsules were prepared by the ionic gelation technique, using carbopol-941 as self-swelling polymer. A 33 Full factorial design was used to study the effect of polymer concentration, Drug complex and sodium alginate. The formed microcapsules were subjected to various evaluation tests such as drug encapsulation efficiency, in vitro drug release...

  19. Design and development of gastro retentive drug delivery System of tramadol hydrochloride

    Rathore, Devashish; Dahima, Rashmi
    The present investigation concerns the development of floating tablets of tramadol hydrochloride, which after oral administration are designed to prolong the gastric residence time; improves the drug bioavailability, reduces drug waste and diminish the side effects of drug. The D-optimal experimental design was employed to evaluate contribution of hydroxypropyl methyl cellulose (HPMC) K4M concentration, lactose concentration and kollidone SR concentration on drug release from floating tablets. Tablets were prepared using direct compression technique. Formulations were evaluated for in vitro buoyancy and drug release study using United States Pharmacopoeia (USP) 24 paddle type dissolution apparatus using 0.1 N HCl as dissolution...

  20. Development and evaluation of naproxen sodium 250 Mg effervescent tablets

    Hanif, Muhammad; Muhammad, Iyad N.; Effet, Wajhia; Yousuf, Rabia I.; Ghiasuddin, Muhammad; Shoaib, Harris M.
    Effervescent tablets have always been convenient, simple and measured dosage form. The phenomenon of carbonation, in this type of dosage form, accelerates the solubility and enhances the bioavailability of the drug and the addition of flavorant also masks the objectionable taste of the medicament in a more patient compliant way. The present study focuses on developing a new, simple, cost effective formulation of naproxen sodium 250 mg as an effervescent tablet using direct compression technique. Nine different trial formulations of naproxen 250 mg were designed with varying proportions of sodium carbonate, sodium bicarbonate, citric acid and PEG 6000 and were...

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