Recursos de colección

Universidad Nacional de La Plata (74.783 recursos)

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vol. 31, no. 03

Mostrando recursos 1 - 20 de 22

  1. Quantitative determination and pharmacokinetics of salvianolic acid l, a novel phenolic acid constituent from salvia miltiorrhiza, in rat plasma by high-performance liquid chromatography

    Zhao, Yongyue; Chu, Yang; Li, Wei; Guo, Jiahua; Wang, Xiangyang; Ma, Xiaohui; Jin, Yuanpeng; Aa, Jiye; Wang, Guangji
    A simple, rapid and selective HPLC method was developed for the determination of a novel phenolic acid constituent in rat plasma, salvianolic acid L (SAL), extracted from the dried root of Salvia miltiorrhiza (Danshen). Plasma samples were extracted by ethyl acetate after addition of the internal standard tinidazole. The appropriate separations were achieved using a C18 column with the mobile phase composed of a mixture of acetonitrile/water/formic acid (35:65:0.1, v/v/v) at the flow rate of 0.8 mL/min, and the wavelength of determination by diode-array detector (DAD) detection was 327 nm. Good linearity (r = 0.9996) was obtained within the concentration...

  2. Phytochemical, toxicity and microbiological activity study of tynanthus micranthus corr

    Cansian, Fernanda C.; Miguel, Marilis D.; Miguel, Obdúlio G.; Miyasaki, Cristina M.S.; Lima, Cristina P.; Kalegari, Milena; Merino, Francis J. Z.; Souza, Angela M.; Cogo, Laura L.
    Tynanthus micranthus, a plant species belonging to family Bignoniaceae, is widely used on the northwest region of Paraná state as stimulant and aphrodisiac. The lack of studies about this specie motivated the research for phytochemical evaluation and some biological activities of the plant. Toxicity studies of the ethanolic extracts and fractions of the species investigated were performed in Artemia salina eggs in addition to antimicrobial activity of the ethanolic extracts against species of some bacteria strains. The results indicated the presence of β-sitosterol steroid in the hexane fraction and apigenin flavone in the chloroform fraction, both isolated from the stalk....

  3. A simple RP-HPLC method for the determination of clotrimazole from acrylic nanocapsule suspensions

    Santos, Sara S.; Ferreira, Luana M.; Schaffazick, Scheila Rezende; Cruz, Leticia
    A simple and fast HPLC method has been validated for the determination of clotrimazole in nanocapsule suspensions prepared from an acrylic polymer, Eudragit RS100®. Chromatographic runs were performed on a RP C18 column with a mobile phase comprising methanol and water (90:10, v/v) at a flow rate of 1.0 mL/min with UV detection at 229 nm. The method was shown to be specific, linear (r = 0.9987), precise and accurate in a concentration range of 2.0-10.0 μg/mL. The proposed method can be successfully used to quantify clotrimazole content in acrylic nanocapsule formulations, as well as to estimate drug encapsulation efficiency.

  4. Pharyngoamygdalitis treatment in San Luis City, Argentina

    De Pauw, María C.; Panini, Alicia, del; Rapisarda, Ana M. P.; Valsecia, Mabel E.; Calderón, Claudia P.
    The aim of this research was to determine and compare the distribution by sex and age of pharyngoamygdalitis cases and analyze quantitatively and qualitatively the applied treatments in Hospitals A (HA) and B (HB) of San Luis city, Argentina. An observational, descriptive, transversal and indication-prescription’s study was carried out. Diagnoses and drugs were classified and the prescriptions were analyzed. Amoxicillin in HA and penicillin G benzathine in HB were mainly prescribed. Antihistamines, mucolytics and antitussives of non-elevated therapeutic value were used. There was widespread use of dipyrone, two NSAIDs were administered simultaneously and non-specific drugs were prescribed. The use of...

  5. Free radical scavenging and anti-edematogenic activities of essential oil obtained from trichilia silvatica dc. (meliaceae) leaves

    Formagio, Anelise Samara Nazari; Santos, Luiz A. C. dos; Cardoso, Claudia A. L.; Silva, Magaiver A.; Zárate, Nestor A. H.; Vieira, Maria do C.; Kassuya, Cândida A. L.; Caramão, Elina B.
    The present study was focused in evaluating the chemical composition, the anti-edematogenic and antioxidant activities of essential oil obtained from Trichilia silvatica (EOTS) leaves. The EOTS was extracted by hydrodistillation and their analyses were performed by GC/MS. The main compounds identified in the EOTS were sesquiterpenes. Furthermore, the EOTS exhibited antioxidant and in vivo anti-inflammatory activity. The oral administration of EOTS (100 and 300 mg/kg), significantly inhibited the carrageenan (Cg) induced rat paw edema. The observed inhibitions were 54 ± 7 and 49 ± 6 % (100 mg/kg) for EOTS and 68 ± 6 % and 66 ± 11 %...

  6. A novel bucco-vaginal controlled release drug delivery system of miconazole nitrate for candidiasis-design and evaluation

    Pendekal, Mohamed S.; Tegginamat, Pramod K.
    A variety of approaches have been studied in the past to overcome the problems encountered with the delivery of antifungal, for effective treating of oral and vaginal candidiasis. In this study, a novel mucoadhesive tablets with pH-independent drug release characteristic was prepared by chitosan and carbopol® 71G interpolymer complex (IPC) claims for multipurpose use. Precipitation method is employed for preparation of IPC followed by characterization with Fourier transform infrared spectroscopy (FTIR) and Differential scanning calorimeter (DSC). Bucco-Vaginal Miconazole nitrate (MN) compacts were prepared by direct compression using IPC. The formulations were tested for physicomechanical properties, in vitro drug release (buccal...

  7. Toxicity study of piroxicam in broilers

    Awan, Asif F.; Ashraf, Muhammad; Malik, Abdul; Akram, Abdul; Murtaza, Ghulam
    This project was designed for the evaluation of different effects of toxic dosage levels of piroxicam in broiler chickens. For this project, one hundred healthy broiler chickens were purchased from the market and were reared up to 28 days. These fifty birds were divided into two groups A and B, having twenty five birds in each group. On day 29th , group A was medicated with piroxicam twice a day at dose rate of 1 mg/kg body weight intra-muscularly (I/M) for four days. Birds from group B were kept as control. Feed and water were provided ad libitum. A physical...

  8. Biological activities of arrabidaea chica (Bonpl.) B. Verl. Leaves

    Amaral, Raquel R. do; Santos, Amanda A. D.; Saravia, Amarillis; Botas, Gisele da S.; Cruz, Rodrigo A.S.; Fernandes, Caio P.; Rocha, Leandro; Boylan, Fábio
    Leaves of Arrabidaea chica (Bonpl.) B. Verl., Bignoniaceae, are commonly used in the Amazon region as anti-inflammatory, antimicrobial, astringent and as a wound healing agent. In the Southeast Region of Brazil, this species is used for urinary infection treatment. Ethanol extract from leaves of Arrabidaea chica and its fractions were prepared and tested for antioxidant, cytotoxic, antibacterial and diuretic activities. All samples, with the exception of the hexane fraction, showed a potential antioxidant activity. Hexane, ethyl acetate fraction and luteolin, a flavone isolated from dichlorometane fraction, demonstrated diuretic activity. None of the samples assayed showed cytotoxicity or antimicrobial activity. Therefore, our...

  9. Nanotechnology in hormone replacement therapy: Safe and efficacy of transdermal estriol and estradiol nanoparticles after 5 Years Follow-Up Study

    Gonzaga, Luiz W.; Botelho, Marco A.; Queiroz, Dinalva; Fechine, Pierre B.; Azevedo, Eduardo; Morais, Angélica; Ruela, Ronaldo; Lyra, Aarão; Gomes, Samela; Quintans Júnior, Lucindo José; Freire, Rafael
    This study aimed to evaluate the safety and efficacy of a novel protocol of transdermal Hormone Replacement Therapy (HRT) based on a nanostructured formulation of Estriol (0.1 %) + Estradiol (0.25 %) restoring serum levels and relieving menopausal symptoms. We evaluated 122 women with mean age of 56.88 (± 6.27) as part a longitudinal prospective study on post-menopausal women with natural menopause, received in the right forearm a transdermal formulation of (EE) daily for 60 months. Clinical parameters including the degree of satisfaction with symptomatic relief, serum concentrations of estradiol, weight, blood pressure, and bilateral mammography BI-RADS were compared between...

  10. Biotransformation of Oleanolic Acid by Penicillium melinii

    Liu, Dailin; Ai, Ting; Yan, Huijun; Liu, Ying; Liu, Hao; Chen, Hong
    Oleanolic acid (1) is a bioactive compound widely distributed in nature. Microbial transformation is an effective way to alter the structures of compounds. The result of biotransformation of oleanolic acid was carried out in this paper. Four transform products (2-5) from 1 by Penicillium melinii were isolated. Their structures were established by spectral data interpretation as 21β-hydroxyoleanolic acid (2), 21α-hydroxyoleanolic acid (3), canthic acid (4), and 7α, 21β-dihydroxyl oleanolic acid (5). Compound 3 had stronger antibacterial activities against MRSA and Staphylococcus aureus than the substrate. It was reported for the first time that the hydroxylation products of oleanic acid can...

  11. Solution Thermodynamics of Sodium Diclofenac in Ethanol + Water Mixtures and Correlation with the Jouyban-Acree Model

    Almanza, Fernán; Holguín, Andrés R.; Delgado, Daniel R.; Martínez, Fleming; Jouyban, Abolghasem
    The solubility of sodium diclofenac (NaDIC) in several ethanol + water mixtures was determined at 293.15 K to 313.15 K. The thermodynamic functions Gibbs energy, enthalpy, and entropy of solution were obtained from these solubility data by using the van’t Hoff and Gibbs equations. Thermodynamic quantities of mixing were also obtained by means of the drug properties of fusion. In contrast to other electrolyte drugs, NaDIC exhibits low solubility and the greatest value was obtained in the mixture 0.80 in mass fraction of ethanol. A non-linear enthalpy–entropy relationship was observed from a plot of enthalpy vs. Gibbs energy of solution....

  12. Antihyperglycemic effect of casearia sylvestris leaves extract in streptozotocin induced diabetic rats

    Buccioli, Paulo T.; Bortolin, Raul H.; Furini, Gleison T.; Cincotto, Danielli U.; Sousa, Janaína H.; França, Suzelei C.; Oliveira, Luciana; Pereira, Paulo S.
    The antihyperglycaemic effect of Casearia sylvestris aqueous extract (CS) and its flavonoidic fraction using in vivo models in streptozotocin-induced diabetic rats was evaluated. CS (500 mg/kg) reduced blood glucose in rats three weeks after a single oral dose. The blood glucose significantly decreased in a test of tolerance to insulin. Phytochemical investigation on TLC, HPLC-DAD and NMR spectral analysis of extract and fractions confirmed the presence of flavonoids. These results direct the hypothesis that CS improves glucose metabolism by reducing insulin resistance, and it may be useful for the treatment of type 2 diabetes.

  13. Cleaning Validation: A Case Study Involving Dexamethasone Cream

    Freitas, Márcia R.; Soares Sobrinho, José L.; Granjeiro Júnior, Severino; Oliveira, Antônio D. P.
    In order to achieve a reliable degree of quality, the pharmaceutical industry needs to introduce a quality control system that includes validation of the cleaning of equipment. This study carried out cleaning validation on the dexamethasone cream production line. The validation study involved evaluation of the cleaning procedures for the dexamethasone cream production line. Samples were collected at thirteen points involving the reactor, the colloidal mill, the industrial blender, the mixer and the packaging machine, for each of the batches. Samples were collected for study of the residue of the principle active ingredient, of detergent, and for microbiological analysis. Cleaning...

  14. Protective Activities of Cistanoside A on CCl4 Induced Hepatotoxicity in Mice

    Luo, Huiying; Wang, Lijuan; Li, Juan; Zhu, Lijuan
    To evaluate the protective efficacy of Cistanoside A (C.A), a phenylethanol glycoside isolated from Cistanche deserticola, on CCl4 induced hepatotoxicity in mice, 50 animals were divided into five different protocols, and hepatic functional index were detected by diagnostic kits. Histological changes were compared by H&E stain. Activities of mitochondrial antioxidant enzymes (GST, SOD, and CAT) and respiratory marker enzymes (MDH, SDH, NADH dehydrogenase, and cytochrome c oxidases) were measured. To confirm the effect of C.A on free radical, tests on the free radical scavenging activities were also carried out in vitro. We found treatment with C.A (10, 20 mg/kg o.p....

  15. Metabolism and Excretion Study of Daphnoretin in Rats after Oral Administration

    Sun, Lixin; Sun, Changshan; Guo, Junyang; Yang, Wengen; Jia, Yurong; Tong, Lijin
    The metabolism and excretion profile in rats were investigated after a single dose of daphnoretin. Metabolites of daphnoretin in rats were characterized by HPLC-MS n analysis. A HPLC-UV method was developed to determine the concentration of daphnoretin in rat urine, feces and bile. Daphnoretin was biotransformed via conjunctive and oxidative pathways to three detected metabolites. The structures of these metabolites were tentatively identified. The cumulative excretion percentage of daphnoretin in urine, feces and bile of rats was 0.13, 52.7, and 0.018 %, respectively. All the metabolites and excretion data are reported for the first time.

  16. Densitometric Determination of Risedronate Sodium in Tablets

    Sangshetti, Jaiprakash; Sakhare, Esha; Wavhal, Parmeshwar; Wakte, Pravin S.; Shinde, Devanand B.
    An HPTLC method for analysis of risedronate sodium in bulk and pharmaceutical formulation has been established and validated. The analyte was separated on aluminium plates precoated with silica gel 60 F254 . The mobile phase was water-acetontrile-ammonia solution 9.3:0.40:0.3 (v/v). Quantification was done by densitometric scanning at 262 nm. Response was a linear function of risedronate asdium concentration in the range of 5 to 25 μg/mL. The limit of detection and quantification for risedronate sodium were 0.86 and 3.03 μg/mL respectively. Average recovery of risedronate sodium was 99.31, which shows that the method was free from interference from excipients present...

  17. Simultaneous Determination of Three Bioactive Compounds in Traditional Chinese Medicine Patent Prescription Zuojin Pill by HPLC

    Cai, Jinzhang; Huang, Zhenzhen; Chen, Chan; Xu, Ren-ai; Lin, Chongliang; Lin, Guanyang; Wang, Xianqin
    A high-performance liquid chromatography (HPLC) method coupled with ultraviolet (UV) detection has been developed and validated for simultaneous quantification of berberine hydrochloride, evodiamine and rutaecarpine in Zuojin Pill. Chromatographic separation were achieved on a ODS-C18 column (250×4.6 mm i.d., 5.0 μm particle) through a 25 min gradient delivery of a mixture of A (acetonitrile) and B (0.3 % phosphoric acid-0.3 % triethylamine) at a flow rate of 0.8 mL/min at 40 °C. All of the analytes showed good linearity (r > 0.999). The method was validated for repeatability, precision, stability, accuracy and selectivity. The validated method was applied to quality...

  18. In Vitro Release Studies of Diclofenac Potassium Tablet from Pure and Blended Mixture of Hydrophilic and Hydrophobic Polymers

    Tariq, Imran; Mumtaz, Ahmad M.; Saeed, Tariq; Shah, Pervaiz A.; Raza, Syed A.; Jawa, Najam ul Hassan; Ali, Muhammad Yasir; Abbas, Ghulam
    The purpose of the present study was to evaluate the effect of pure and blended mixtures, with different compositions of hydroxy propyl methyl cellulose (HPMC) and carnauba wax (CW) on the release of diclofenac potassium from matrix tablets. Fifteen different matrix tablet formulations were prepared by direct compression process by using Carver Hydraulic laboratory press having 13 mm flat dies set at constant pressure. The paddle dissolution apparatus II (Curio DL 2020) was used to assess the dissolution of drug in phosphate buffer, pH 7.4 for 8 h. The release data was fitted to different release models. Zoom stereo micrography was...

  19. Mathematical Modeling of Drug Release of Novel Extended-Release Formulations of Tizanidine Hydrochloride

    Naeem, Muhammad; Akhter, Khalid P.; Ahmad, Ajaz; Aamir, Muhammad N.; Aslam, Zartasha
    Six extended-release formulations of tizanidine hydrochloride were prepared by direct compression technique using hydroxypropylmethylcellulose (HPMC) and ethylcellulose (EC). The drug release from these tablets was evaluated by analyzing the samples at 227 nm. Both model dependent and independent mathematical approaches were applied to investigate the extended release pattern and mechanism of tizanidine hydrochloride. About 87.8, 74.4, 85.1, 74.8, 70.8, and 56.2 % drug was released from F1 to F6 , respectively, in 12 h. Drug release kinetics indicated that drug release was best explained by Higuchi equation. The values of ‘n’ from Korsmeyer-Peppas model (0.45 to 0.89) supported anomalous or...

  20. Current Clinical Practices in Pakistan and Hospital Pharmacist’s Perception towards Their Role: A Qualitative Approach

    Azhar, Saira; Hassali, Mohamed A.; Murtaza, Ghulam; Hussain, Izhar
    The professional role of the pharmacist in hospital setting is changing from a focus on preparation of medication to patient oriented approach and now pharmacist assists the patient to get best possible results from medication. The aim of this study was to evaluate the perception of hospital pharmacists towards their role in the healthcare system of Pakistan. A qualitative study design was adopted. Face to face interviews were conducted using a semi structure interview guide. A total of nineteen interviews were conducted and become the part of the study. All the interviews were transcribed verbatim and thematically analyzed for its...

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