Recursos de colección

Repositorio Institucional de la Universidad de Cordoba :: Spain (18.741 recursos)

El repositorio recoge todo tipo de materiales digitales: artículos de revistas, comunicaciones a congresos, tesis doctorales, documentos de trabajo, materiales docentes y objetos de aprendizaje, así como los productos digitales del patrimonio bibliográfico de la Universidad de Córdoba.

DBCFI-Artículos, capítulos...

Mostrando recursos 1 - 20 de 54

  1. Rab18 Dynamics in Adipocytes in Relation to Lipogenesis, Lipolysis and Obesity

    Pulido, Marina R.; Díaz-Ruiz, Alberto; Jiménez-Gómez, Yolanda; García-Navarro, Socorro; Gracia-Navarro, F.; Tinahones, Francisco J.; López-Miranda, José; Frühbeck, Gema; Vázquez Martínez, Rafael; Malagón, María M.
    Lipid droplets (LDs) are organelles that coordinate lipid storage and mobilization, both processes being especially important in cells specialized in managing fat, the adipocytes. Proteomic analyses of LDs have consistently identified the small GTPase Rab18 as a component of the LD coat. However, the specific contribution of Rab18 to adipocyte function remains to be elucidated. Herein, we have analyzed Rab18 expression, intracellular localization and function in relation to the metabolic status of adipocytes. We show that Rab18 production increases during adipogenic differentiation of 3T3-L1 cells. In addition, our data show that insulin induces, via phosphatidylinositol 3-kinase (PI3K), the recruitment of Rab18 to the surface of...

  2. Complex I-Associated Hydrogen Peroxide Production Is Decreased and Electron Transport Chain Enzyme Activities Are Altered in n-3 Enriched fat-1 Mice

    Hagopian, Kevork; Weber, Kristina L.; Hwee, Darren T.; Van Eenennaam, Alison L.; López-Lluch, Guillermo; Villalba, José Manuel; Burón, Isabel; Navas Lloret, Plácido; German, Bruce; Watkins, Steven; Chen, Yana; Wei, Alfreda; McDonald, Roger B.; Ramsey, Jon J.
    The polyunsaturated nature of n-3 fatty acids makes them prone to oxidative damage. However, it is not clear if n-3 fatty acids are simply a passive site for oxidative attack or if they also modulate mitochondrial reactive oxygen species (ROS) production. The present study used fat-1 transgenic mice, that are capable of synthesizing n-3 fatty acids, to investigate the influence of increases in n-3 fatty acids and resultant decreases in the n-6:n-3 ratio on liver mitochondrial H2O2 production and electron transport chain (ETC) activity. There was an increase in n-3 fatty acids and a decrease in the n-6:n-3 ratio in liver mitochondria from the fat-1...

  3. Role of SST, CORT and ghrelin, and its receptors at the endocrine pancreas

    Chanclón García, Belén; Martínez-Fuentes, Antonio J.; Gracia-Navarro, F.

  4. Método de obtención de datos útiles para predecir la respuesta al tratamiento de la Hepatitis C

    Peña Martínez, José; Torre-Cisneros, Julián de la; Rivero Román, Antonio; González Fernández, Rafael A.; Manzanares Martín, Bárbara; Rivero Juárez, Antonio; Camacho Espejo, Ángela
    La presente invención proporciona un método de obtención de datos útiles para predecir cual será la respuesta de un paciente al tratamiento de la hepatitis C, específicamente al tratamiento con interferón pegilado y ribavirina (Peg-IFN/RBV). Los autores de la invención han encontrado un biomarcador génico que permite predecir la respuesta al tratamiento de la hepatitis C. Esto es de gran utilidad en la práctica clínica para determinar la terapia a aplicar en cada paciente, dentro de los que se viene en denominar “medicina individualizada”

  5. La ecoinnovación en el sector agroalimentario: ¿qué puede aportar el Campus?

    Castaño, Justo P.; Domínguez García-Barquero, Ricardo

  6. Immunohistochemical analysis of ß3 integrin (CD61): expression in pig tissues and human tumors

    Moreno, Ángela; Lucena, Concepción; López, A.; Garrido, Juan J.; Pérez de la Lastra, J.M.; Llanes Ruiz, Diego
    CD61 is a membrane glycoprotein that associates with CD41 (aIIb) to form the heterodimeric complex gpIIb/IIIa (CD41/CD61), predominantly expressed in platelets and megakariocytes. CD61 or ß3 integrin also associates with av (CD51) to form the vitronectin receptor, which is expressed in many tissues. We have used a monoclonal antibody against the porcine gpIIIa or CD61 (JM2E5) to study the distribution of this molecule in different normal pig tissues. As in humans, CD61 was broadly expressed in all tissues examined. In the kidney, strong expression of CD61 was observed in epithelial cells from renal tubules. In the testis, CD61 expression was detected in the Leydig cells. However, in liver, CD61 was weak or not...

  7. Hypothalamic mTOR Signaling Mediates the Orexigenic Action of Ghrelin

    Martins, Luis; Fernández-Mayo, Diana; Novelle, Marta G.; Vázquez, María J.; Tena-Sempere, Manuel; Nogeiras, Rubén; López, Miguel; Diéguez, Carlos
    Current evidence suggests that ghrelin, a stomach derived peptide, exerts its orexigenic action through specific modulation of Sirtuin1 (SIRT1)/p53 and AMP-activated protein kinase (AMPK) pathways, which ultimately increase the expression of agouti-related protein (AgRP) and neuropeptide Y (NPY) in the arcuate nucleus of the hypothalamus (ARC). However, there is a paucity of data about the possible action of ghrelin on alternative metabolic pathways at this level. Here, we demonstrate that ghrelin elicits a marked upregulation of the hypothalamic mammalian target of rapamycin (mTOR) signaling pathway. Of note, central inhibition of mTOR signaling with rapamycin decreased ghrelin’s orexigenic action and normalized the mRNA expression of AgRP and...

  8. Intensification of Antiretroviral Therapy with a CCR5 Antagonist in Patients with Chronic HIV-1 Infection: Effect on T Cells Latently Infected

    Gutiérrez, Carolina; Díaz, Laura; Vallejo, Alejandro; Hernández-Novoa, Beatriz; Abad, María; Madrid, Nadia; Dahl, Viktor; Rubio, Rafael; Moreno, Ana M.; Dronda, Fernando; Casado, José Luis; Navas, Enrique; Pérez-Elías, María Jesús; Zamora, Javier; Palmer, Sarah; Muñoz Blanco, Eduardo; Muñoz-Fernández, María Ángeles; Moreno, Santiago
    Objective: The primary objective was to assess the effect of MVC intensification on latently infected CD4+ T cells in chronically HIV-1-infected patients receiving antiretroviral therapy. Methods: We performed an open-label pilot phase II clinical trial involving chronically HIV-1-infected patients receiving stable antiretroviral therapy whose regimen was intensified with 48 weeks of maraviroc therapy. We analyzed the latent reservoir, the residual viremia and episomal 2LTR DNA to examine the relationship between these measures and the HIV-1 latent reservoir, immune activation, lymphocyte subsets (including effector and central memory T cells), and markers associated with bacterial translocation. Results: Overall a non significant reduction in the size of the latent reservoir...

  9. The Somatostatin Analogue Octreotide Inhibits Growth of Small Intestine Neuroendocrine Tumour Cells

    Li, Su-Chen; Martijn, Cécile; Cui, Tao; Essaghir, Ahmed; Luque, Raúl M.; Demoulin, Jean-Baptiste; Castaño, Justo P.; Öberg, Kjell; Giandomenico, Valeria
    Octreotide is a widely used synthetic somatostatin analogue that significantly improves the management of neuroendocrine tumours (NETs). Octreotide acts through somatostatin receptors (SSTRs). However, the molecular mechanisms leading to successful disease control or symptom management, especially when SSTRs levels are low, are largely unknown. We provide novel insights into how octreotide controls NET cells. CNDT2.5 cells were treated from 1 day up to 16 months with octreotide and then were profiled using Affymetrix microarray analysis. Quantitative real-time PCR and western blot analyses were used to validate microarray profiling in silico data. WST-1 cell proliferation assay was applied to evaluate cell growth of CNDT2.5 cells in the...

  10. Role of Endogenous Cortistatin in the Regulation of Ghrelin System Expression at Pancreatic Level under Normal and Obese Conditions

    Chanclón García, Belén; Luque, Raúl M.; Córdoba-Chacón, José; Gahete Ortiz, Manuel D.; Pozo-Salas, Ana I.; Castaño, Justo P.; Gracia-Navarro, F.; Martínez-Fuentes, Antonio J.
    Ghrelin-system components [native ghrelin, In1-ghrelin, Ghrelin-O-acyltransferase enzyme (GOAT) and receptors (GHS-Rs)] are expressed in a wide variety of tissues, including the pancreas, where they exert different biological actions including regulation of neuroendocrine secretions, food intake and pancreatic function. The expression of ghrelin system is regulated by metabolic conditions (fasting/obesity) and is associated with the progression of obesity and insulin resistance. Cortistatin (CORT), a neuropeptide able to activate GHS-R, has emerged as an additional link in gut-brain interplay. Indeed, we recently reported that male CORT deficient mice (cort2/2) are insulin-resistant and present a clear dysregulation in the stomach ghrelin-system. The present work was focused at analyzing the...

  11. Modifications of plasma proteome in healthy humans fed o a coenzymen Q-10 supplemented diet

    Santos-González, Mónica; López-Miranda, José; Pérez-Jiménez, Francisco; Villalba, José Manuel
    Comunicaciones a congresos

  12. Factores limitantes del rendimiento físico del toro bravo durante la lidia

    Agüera Buendía, Estrella; Requena Domenech, Francisco
    Con este trabajo se persigue aportar nuevas ideas sobre la fi siopatología del ejercicio en el toro bravo, siendo el objetivo principal, valorar los cambios metabólicos que se presentan deforma secundaria a las lesiones producidas durante la lidia como factores limitantes del rendimiento físico. Se obtuvieron muestras de sangre antes y después de la lidia y biopsias musculares después de la lidia de 30 toros de 4-5 años de edad pertenecientes a ganaderías de las provincias de Córdoba, Sevilla y Badajoz. La sangre fue separada en dos fracciones: una para el estudio desangre entera y otra para el estudio bioquímico...

  13. Diferencias en el perfil enzimático muscular y respuesta metabólica a la lidia en toros de uno a tres años de edad

    Muñoz, A.; Castejón Montijano, F.; Agüera Buendía, Estrella
    The metabolic response to exercise is partially linked to muscle enzyme profile, and both factors might be influenced by ageing. This research aims: 1) To analyze the differences in muscle characteristics of fighting bulls of different ages; and 2) To assess whether the muscle enzyme profile is affected by ageing in the same way it affects other bovine breeds. Muscle biopsies from gluteus medius and semitendinosus muscles were withdrawn immediately after bullfighting, at depths of 3 and 5 cm, from a total of 24 animals, belonging to three age groups: A (1-year-olds; n=6), B (2-year-olds; n=6) and C (3-year-olds; n=12). Muscle concentrations of glycogen,...

  14. A Novel Human Ghrelin Variant (In1-Ghrelin) and Ghrelin-O-Acyltransferase Are Overexpressed in Breast Cancer: Potential Pathophysiological Relevance

    Gahete Ortiz, Manuel D.; Córdoba-Chacón, José; Hergueta-Redondo, Marta; Martínez-Fuentes, Antonio J.; Kineman, Rhonda D.; Moreno-Bueno, Gema; Luque, Raúl M.; Castaño, Justo P.
    The human ghrelin gene, which encodes the ghrelin and obestatin peptides, contains 5 exons (Ex), with Ex1-Ex4 encoding a 117 amino-acid (aa) preproprotein that is known to be processed to yield a 28-aa (ghrelin) and/or a 23-aa (obestatin) mature peptides, which possess biological activities in multiple tissues. However, the ghrelin gene also encodes additional peptides through alternative splicing or post-translational modifications. Indeed, we previously identified a spliced mRNA ghrelin variant in mouse (In2-ghrelin-variant), which is regulated in a tissue-dependent manner by metabolic status and may thus be of biological relevance. Here, we have characterized a new human ghrelin variant that contains Ex0-1, intron (In) 1,...

  15. Metabolic Impact of Adult-Onset, Isolated, Growth Hormone Deficiency (AOiGHD) Due to Destruction of Pituitary Somatotropes

    Luque, Raúl M.; Lin, Quig; Córdoba-Chacón, José; Subbaiah, Papasani V.; Buch, Thorsten; Waisman, Ari; Vankelecom, Hugo; Kineman, Rhonda D.
    Growth hormone (GH) inhibits fat accumulation and promotes protein accretion, therefore the fall in GH observed with weight gain and normal aging may contribute to metabolic dysfunction. To directly test this hypothesis a novel mouse model of adult onset-isolated GH deficiency (AOiGHD) was generated by cross breeding rat GH promoter-driven Cre recombinase mice (Cre) with inducible diphtheria toxin receptor mice (iDTR) and treating adult Cre+/2,iDTR+/2 offspring with DT to selectively destroy the somatotrope population of the anterior pituitary gland, leading to a reduction in circulating GH and IGF-I levels. DT-treated Cre2/2,iDTR+/2 mice were used as GH-intact controls. AOiGHD improved whole body insulin sensitivity in both...

  16. Relaciones dentro de la subfamilia Papilionoideae a la luz de los datos palinológicos

    Prados, A.M.; Porras, A.; Ubera Jiménez, José Luis; Domínguez Vilches, E.
    En este t rabajo se realiza un análisis numérico de la semejanza pol ínica de 216 especies de Leguminosae de Andalucía Occi de ntal ~ dichas semejanzas se representan según un dendrograma y a partir del cual se comentan l as implicaciones taxonómicas.

  17. Estudio palinológico de Carlina L. y Atractylis L. en Andalucía Occidental

    Tormo Molina, Rafael; Ubera Jiménez, José Luis; Domínguez Vilches, E.; Guerrero, F.H.
    En este trabaj o se estuéia l a palinologia de s eis espe c i e s de Carlina L. y Atractylis L. (C . racer:to•a L., c . corymbosa !. .. , C. l anata L. , A. hurnilis L. A. gwrnifera L., A. cancellala L.) mediante el ana b :as a <~icros copia óptica, elec-:rónica de bar:-ido y t ransmisión. En el se dcoc r1ben dos t:pos polin1cos: "Car lina" (Carlina ssp. , A. gWD111ifere y A. humilis) y 11Atractylis1' (A. cancel lata) . Por último se conen.ta la delimi 'taciún g~ner i e o Ce los taxoncs estudi ados , en base a...

  18. Aproximación diagnostica a la disminución de rendimiento en el caballo de deporte

    Requena Domenech, Francisco; Agüera Buendía, Estrella
    El presente trabajo se estructura de una forma práctica, se va a exponer la manera de actuar del clínico veterinario ante un caso de disminución del rendimiento de un caballo de deporte. La anamnesis es lo primero que tenemos que realizar, cuando se nos presenta un caso de disminución del rendimiento. Una anamnesis bien realizada es fundamental para poder llegar más tarde junto al examen físico y pruebas complementarias a un diagnóstico preciso. Deberemos de conocer si el caballo ha tenido alguna vez buen rendimiento o si nunca lo tuvo. A la anamnesis le seguirá la realización de un examen físico tanto en reposo como en movimiento. El examen en reposo constará de: examen respiratorio, del aparato cardiocirculatorio, neurológico, hemograma...

  19. Genética de la caseína de la leche en el bovino Frisón

    Requena Domenech, Francisco; Agüera Buendía, Estrella
    Hoy en día la Industria Alimentaria tiene un importante objetivo buscar una mejora en la composición de la leche para elaborar productos de mayor calidad, puesto que es lo que demanda el consumidor. Para conseguir esta mejora, es necesario incrementar la proteína de la leche, especialmente la caseína, que se considera la de mejor calidad. El ganado bovino produce una media de 3,5% de proteína en la leche, del cual, el 80% son caseínas (α, β y ) y el 20% proteínas séricas (α-lactoalbúmina y β- lactoglobulina).En la producción de caseínas de un animal intervienen varios factores, como la alimentación, la edad del animal, número de lactación, número de ordeños, genética del animal, etc. Por lo tanto la...

  20. Diseño de una nueva asignatura dirigida al aprendizaje, integración y evaluación de competencias transversales en el grado de Medicina para la aplicación y mejora de su perfil laboral

    Solana Lara, Rafael; Jiménez Reina, L.; Labella Quesada, Fernando; Peña Amaro, J.
    La propuesta presenta la creación de una nueva asignatura en el grado de Medicina: Aplicación Clínica de las Ciencias Médicas Básicas –cuyo desarrollo ha implicado una inversión de 6.000 euros– que se imparte en el segundo semestre del segundo curso de la titulación. Se trata de una asignatura dirigida en exclusiva al aprendizaje y evaluación de las competencias transversales que, según los autores de la misma, supondrá una mejora para el alumnado a la hora de incorporarse al mercado de trabajo

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