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Biblioteca Digital do IPB (53.442 recursos)

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Mostrando recursos 1 - 20 de 31

  1. Antioxidant activities of five Lamiaceae plants

    Pereira, Olívia R.; Perez, María J.; Macias, Rócio I.R.; Marín, Jose J.G.; Cardoso, Susana M.
    In the last decades, oxidative stress has been recognized as a key process in the physiopathology of several diseases. Consequently, the search for new antioxidant compounds, as well as new antioxidant sources, has increased exponentially. The Lamiaceae family encloses many plant species which are potential sources of antioxidant compounds. The present study evaluates the antioxidant activity of phenolic enriched extracts of Lamium album, Leonurus cardiaca, Lavandula dentata, Mentha aquatica and Thymus citriodorus. The antioxidant activity of the hydroethanolic plant extracts was estimated by the in vitro measurement of their 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) scavenging potential and reducing power assays. Additionally, the protective...

  2. Polyphenols characterization and toxicological evaluation of pterospartum tridentatum leaf water extracts

    Ferreira, Fernanda M.; Peixoto, Francisco P.; Pereira, Olívia R.; Dinis, Lia T.; Domingues, M.R.M.; Palmeira, Carlos M.; Cardoso, Susana M.
    Pterospartum tridentatum Willk. (prickled broom) is an autochthonous and common plant in Portugal. Leaves and stems are normally used in cooking, to flavour rice, roast meat or hunting animals. Leaves are also used as a condiment in fresh salads and, despite of its traditional use, no toxicological evaluation has been performed. P. tridentatum leaves aqueous extract ESI-MS spectrum revealed the presence of several luteolin and isorhamnetin derived phenolic compounds, which can be associated to the health benefits claimed for this plant species. Still, P. tridentatum leaves extract (up to 100 µg plant extract.mg-1 protein) stimulated state 4 and FCCP-stimulated liver...

  3. Effect of pterospartum tridentatum enriched phenolic extract in mitochondrial bioenergetic functions

    Ferreira, Fernanda M.; Peixoto, Francisco P.; Pereira, Olívia R.; Dinis, Lia T.; Domingues, M.R.M.; Crespi, António J.L.; Palmeira, Carlos M.; Cardoso, Susana M.
    Pterospartum tridentatum Willk. is an autochthonous and common plant in Portugal, locally known as ‘carqueija’. Leaves and stems are normally used in cooking, to flavour rice, roast meat or hunting animals and leaves are also used as condiment in fresh salads. Despite its traditional use, no evaluation in mitochondrial bioenergetics has been performed. P. tridentatum leaf extract phenolic composition was elucidated by electrospray ionization-mass spectrometry (ESI-MS). M. aquatica extract effects were evaluated over mitochondrial membrane potential by using a Clarktype oxygen electrode in the presence of succinate as respiratory substrate. Cytotoxicological effects of plant extracts were evaluated on HepG2 cells...

  4. Portuguese propolis enriched phenolic extract: reactive oxygen scavenging and cytoprotective activities

    Pereira, Olívia R.; Macias, Rócio I.R.; Perez, María J.; Marín, Jose J.G.; Cardoso, Susana M.
    Propolis, a resinous natural product produced by honeybees, is claimed to have a wide range of beneficial activities for human health which have been attributed to its phenolics. In general, phenolics account for approximately half of propolis weight, although its content and composition can greatly vary with propolis geographical origin. This study aims determining the antioxidant and cytoprotective properties of Northeast Portuguese propolis. The propolis hydroethanolic purified extract (PPE) was obtained by extraction at 70 °C/1 h and recovery onto SPE C18 cartridges. Total phenolic content and identification of the main phenolics in the PPE were assessed by the Folin...

  5. Mentha aquatica L. extract affects mitochondrial bioenergetics

    Ferreira, Fernanda M.; Pereira, Olívia R.; Cardoso, Susana M.; Oliveira, Paulo J.; Moreno, António J.M.
    Mentha aquatica extracts are commonly used in food flavoring and pharmacology. In the present work, we evaluated the possible effects of Mentha aquatica L. (water mint) ethanolic extract on rat liver mitochondria bioenergetics. Rat liver mitochondria were isolated using conventional protocols. M. aquatica extracts were evaluated on mitochondrial membrane electric potential by using a tetraphenylphosphonium cation (TPP+)-selective electrode, while mitochondrial respiratory activity was evaluated using a Clark-type oxygen electrode, either in the presence of glutamate + malate and succinate as respiratory substrates. Mitochondrial osmotic volume changes were measured by the pseudo-absorbance changes at 520 nm with a suitable spectrophotometer- recorder set...

  6. NO radical scavenging and iNOS expression inhibition by Cytisus multiflorus

    Saraiva, Susana C.; Pereira, Olívia R.; Liberal, Joana; Batista, Maria T.; Cruz, Maria T.; Cardoso, Susana M.
    Historically, extracts and preparations of plants are the basis of traditional medicine and the starting point for the discovery of new therapeutic agents. Cytisus multiflorus is used in folk medicine and it is claimed to have various health benefits, including anti-inflammatory properties. Still, no scientific data regarding this ability has been described for this plant. The present work aims to clarify the antioxidant capacity and the anti-inflammatory mechanisms of Cytisus multiflorus. A purified ethanolic extract was prepared and its antioxidant abilities were evaluated through the DPPH scavenging and reducing power assays. Anti-inflammatory properties were assessed by determining the amounts of...

  7. Phenolic composition of Leonurus cardiaca L.

    Pereira, Olívia R.; Domingues, M.R.M.; Cardoso, Susana M.
    Leonurus cardiaca L., also known as motherwort, is a plant native from central Europe that it is spread in different temperate countries around the world. The plant has been described to exert several beneficial properties and it is presently used in infusions, decoctions, syrups and tinctures, as well as an ingredient of pharmaceutical formulations. Yet, the plant phenolics, which are frequently associated to the claimed health benefits of Leonurus cardiaca L., are not fully clarified. This topic is focused in the present work. For that, an extract of the aerial parts of the plant was obtained with aqueous ethanolic solution...

  8. Is Cytisus multiflorus an antiinflamatory plant?

    Saraiva, Susana C.; Pereira, Olívia R.; Liberal, Joana; Batista, Maria T.; Cruz, Maria T.; Cardoso, Susana M.
    Cytisus multiflorus is a leguminous shrub native from Iberian Peninsula that is distributed in the south-west Mediterranean region. This plant is used in folk medicine and it is claimed to have various health benefits, including anti-inflammatory properties[1]. Yet, the anti-inflammatory usage of C. multiflorus is totally based on the available ethnopharmacological information while no scientific data on this capacity and on molecular targets has been reported for the plant. Hence, the present work aims to clarify the possible anti-inflammatory mechanisms of C. multiflorus. A purified ethanolic extract was prepared and its high antioxidant capacity was confirmed though the DPPH radical...

  9. Body image and anthropometric data in adolescents

    Bonifácio, Carla; Francisco, Raquel; Caldeirita, Cláudia; Lemos, Alexandra; Fernandes, António; Ferro-Lebres, Vera
    Background Body Image reflects a person path, whose perceptions are part of its unit and are determinant for its existence, at each moment. Na individuals perception about his body is affected by others comments, that most of the times leads to insatisfaction with owns body image. At the present moment, studies that correlate real measured anthropometric data, body image perception and satisfac- tion are not known. Objective To evaluate the relation between real anthropometric data and body image perception and satisfaction, in Portuguese adolescents. Methods A descriptive transversal and quantitative study was designed in na education institution located in the North of Portugal. There were evaluated 214 adolescents, with ages between 10...

  10. Influence of socio-demographic issues in body mass index (BMI) of drug addicts in methadone maintenance treatment

    Costa, Ana Filipa; Alves, Daniela; Custódio, Daniela; Natário, Liliana; Andrade, Fernando; Ferro-Lebres, Vera
    The purpose of this study was to investigate the influence of social factors on the nutritional status of drug addicts. 60 drug addicts in methadone maintenance treatment of Integrated Response Center (CRI) of Bragança were evaluated. Nutritional assessment was performed by measuring the weight and height and then calculated the body mass index. The socio-demographic data were collected through a questionnaire constructed for this purpose. PASW 19.0 for Windows. The average body mass index (BMI) was 22.46 ± 3.36 kg/m2 and based on this parameter 13.3% were underweight, 65% normal weight, 20.0% overweight and 1,7% obesity grade I. BMI was significantly...

  11. National health surveys: anthropometric data of Portuguese and Brazilian women: INS 2005–2006 and PNDS 2006

    Costa, Caroline Santos; Marmitt, Luana Patrícia; Meller, Fernanda Oliveira; Ferro-Lebres, Vera
    Background Nutritional problems related to under or overweight in women have been shown itself as a relevant issue to health and quality of life, affecting mainly older women. Objective To compare the anthropometric data of Portuguese and Brazilian women studied on the 4th National Health Inquiry (48 Inque ´rito Nacional de Sau´de, INS-2005-2006, Portugal) and on the National Research of Demography and Health (Pesquisa Nacional de Demografia e Sau´de, PNDS-2006, Brazil). Methods This work is a result of a data comparison between the Portuguese and the Brazilian national inquiries, respectively, INS-2005/ 2006 and PNDS-2006. Both researches represent the population of the countries and were based on transversal studies. The nutritional status...

  12. Phenylethanoids and isoscutellarein derivatives from Lamium album L.

    Pereira, Olívia R.; Domingues, M.R.M.; Silva, Artur; Cardoso, Susana M.
    Lamium album L., a plant commonly known as white dead nettle, has been used in folk medicine in the treatment of several ailments. Some of their benefitial properties are closely related to its phenolic content, however the exact polyphenolic composition is far from being understood. In this context, the present work aims to determine the phenolic composition of an ethanolic extract of L. album using high performance liquid chromatography with diode array detection, electrospray mass spectrometry and nuclear magnetic resonance techniques. The phenolic extract of L. album was mainly composed by the phenylethanoid verbascoside and its isomer isoverbascoside. Still, it also...

  13. Depressive symptoms and alcohol use among adolescents

    Pinto, Isabel C.; Fraga, Sílvia; Ramos, Elisabete
    Alcohol is the most consumed substance by young people, sometimes it’s associated with depressive symptoms. Objective: Assess the factors associated with alcohol use among 13-year-old teenagers, like depressive symptoms. Methods: Data of a population-based cohort of urban teenagers included cross-sectional information of 919 boys and 1016 girls collected by self-reported questionnaire. Depressive symptoms were evaluated using Beck Depressive Inventory II (BDI). The Mann-Whitney test was used to compare medians. Odds ratio (OR) and multiple linear regression (β) were performed to estimate the magnitude of associations with 95% confidence intervals (95%CI). The final model was adjusted for parents’ education, sports, smoking, have friends...

  14. Bioactive properties of Clitocybe alexandri

    Vaz, Josiana A.; Heleno, Sandrina A.; Martins, Anabela; Almeida, Gabriela M.; Vasconcelos, M. Helena; Ferreira, Isabel C.F.R.
    Some mushrooms are known to have strong antioxidant capacity [1]. There is an accepted relationship between the physiopathology of several chronic diseases and oxidative stress. Therefore, the use of foods such as those mushrooms with antioxidant capacity, as phytochemical protectors, may be relevant for the prevention of oxidative stress related diseases such as cancer. Additionally, mushrooms have been described as a source of potential antitumour molecules, making them attractive candidates for drug discovery [2,3]. However, there are no such studies on the Portuguese wild mushroom Clitocybe alexandri .

  15. In vitro growth inhibitory activity of the Portuguese wild mushroom Clitocybe alexandri in human tumour cell lines

    Vaz, Josiana A.; Almeida, Gabriela M.; Martins, Anabela; Vasconcelos, M. Helena; Ferreira, Isabel C.F.R.

  16. Antioxidant activity and growth inhibitory activity of Portuguese wild mushrooms

    Vaz, Josiana A.; Martins, Anabela; Almeida, Gabriela M.; Vasconcelos, M. Helena; Ferreira, Isabel C.F.R.

  17. Nutritional knowledge and calcium intake on health professionals

    Ferro-Lebres, Vera; Almeida de Souza, Juliana; Alves, Lisandra; Jardim, Marta; Pedrosa, Sofia; Mendes, Sónia; Nogueira, Tiago
    It is demonstrated that a better knowledge leads to better eating habits but there are no studies on the relation between NK* and calcium intake. Verify if calcium intake of health professionals is influenced by their NK. 103 workers of the three Hospitals of Oporto filled two previously validated questionnaires Food Frequency Questionnaire (FFQ), General Nutritional Knowledge Questionnaire(GNKQ) and a questionnaire about Socio-demographic data. The relation between NK and calcium intake was analyzed using Kruskal-Wallis test. Individuals that had a calcium intake within DRI’s** had a significantly higher score in GNKQ Total Score and in the Section It appears that NK influences calcium DRIs compliance in health...

  18. Antioxidant activity of thymus citriodorus

    Pereira, Olívia R.; Perez, María J.; Macias, Rócio I.R.; Marín, Jose J.G.; Cardoso, Susana M.
    Background and aims: Thymus citriodorus, or lemon thyme, is used as a medicinal plant in the treatment of asthma and other respiratory diseases, although the mechanism of its beneficial properties is poorly understood. The aim of the present study was to determine the antioxidant ability of the polyphenols present in this plant. Methods: An ethanolic extract was prepared and its antioxidant activity was determined by in vitro measurement of its 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) scavenging potential, reducing power, and protective effects against the generation of reactive oxygen species (ROS) induced by potassium dichromate (5 and 25 μM) in hepatoblastoma HepG2 cells. The...

  19. Thymus citriodorus as a source of antioxidants

    Pereira, Olívia R.; Peres, António M.; Domingues, M.R.M.; Cardoso, Susana M.
    Thymus species are well known as medicinal plants because of their biological and pharmacological properties, which include anti-asthmatic, anti-septic, antimicrobial and antioxidant. It is believed that part of these beneficial effects are due to the volatile constituents of Thymus, and thus, their essential oil composition has been the focus of many investigations. In contrast, there is only a limited number of data on the composition of other bioactive phytochemicals of Thymus and their potential biological effects. The present study aims to elucidate the phenolic composition of an ethanolic extract of Thymus citriodorus, as well as to determine its antioxidant capacity....

  20. Thymus citriodorus: phenolic characterization and antioxidant activity

    Pereira, Olívia R.; Domingues, M.R.M.; Cardoso, Susana M.
    The present work aims to determine the phenolic composition of Thymus citriodorus and to estimate its antioxidant capacity. An ethanolic extract was prepared from the plant and its total phenolic and flavone contents were determined. The phenolic extract was fractionated by reversed-phase HPLC and the major phenolic compounds of each fraction were identified by ESI-MS and MSn analysis, in order to establish the specific phenolic profile of the plant. The total phenolic compounds in the ethanolic extract of Thymus citriodorus accounted for 138,75±13,56 mg/g, and its content of flavones was estimated as 27,30±2,15 mg/g. Moreover, the interpretation of the fragmentation...

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