Hokkaido University Collection of Scholarly and Academic Papers
(65.390 recursos)
HUSCAP (Hokkaido University Collection of Scholarly and Academic Papers) contains peer-reviewed journal articles, proceedings, educational resources and any kind of scholarly works of Hokkaido University.
Mostrando recursos 1 - 20 de 187
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Abnormal Brain MRI Signals in the Splenium of the Corpus Callosum, Basal Ganglia and Internal Capsule in a Suspected Case with Tuberculous Meningitis - Hirotani, Makoto; Yabe, Ichiro; Hamada, Shinsuke; Tsuji, Sachiko; Kikuchi, Seiji; Sasaki, Hidenao
The patient was a 34-year-old man who visited the hospital with chief complaints of headache, fever, and disturbance of consciousness. In view of his clinical condition, the course of the disease, and results of examination, he was diagnosed with viral meningitis and treated accordingly. However, his clinical condition worsened, and MRI revealed abnormal signals in the splenium of the corpus callosum, in the basal ganglia and in the internal capsule, as well as the presence of severe inflammation in the base of the brain. Since he had a high ADA level in the cerebrospinal fluid and was consequently suspected to...
2.
Scapular Winging as a Symptom of Cervical Flexion Myelopathy - Yaguchi, Hiroaki; Takahashi, Ikuko; Tashiro, Jun; Tsuji, Sachiko; Yabe, Ichiro; Sasaki, Hidenao
A 23-year-old man complained of weakness of the right arm that he first noted six years prior to his visit. Neurological examination revealed atrophy and weakness of the triceps and serratus anterior muscle on the right side, which resulted in scapular winging on that side. MRI with neck flexion revealed compression of the cervical cord enabling a diagnosis of flexion myelopathy. Proximal muscle weakness and atrophy in flexion myelopathies including Hirayama disease are extremely rare. Here, we report a case of unilateral, proximal upper limb atrophy with scapular winging, attributed to middle cervical flexion myelopathy.
3.
Morphological and functional analyses of adult onset vitelliform macular dystrophy. - Saito, W.; Yamamoto, S.; Hayashi, M.; Ogata, K.
Aim: To evaluate the morphology and visual function of the macula in eyes with adult onset vitelliform
macular dystrophy (AVMD).
Methods: 12 eyes of six patients with AVMD were examined by ophthalmoscopy, scanning laser ophthalmoscopy
(SLO), optical coherence tomography (OCT), and multifocal electroretinography
(mfERGs). The macular lesions were bilateral in all patients and varied from the typical vitelliform (five
eyes), faded vitelliform changes with retinal pigment epithelium (RPE) atrophy (five eyes), and a normal
fovea associated with small flecks around the macula (two eyes).
Results: SLO demonstrated small abnormal bright spots in the deep retina throughout the posterior
retina in all cases. OCT showed a highly reflective fusiform...
4.
Inhibition of nuclear factor-kappa B activation attenuates hydrogen peroxide-induced cytotoxicity in human lens epithelial cells - Jin, Xue-Hai; Ohgami, Kazuhiro; Shiratori, Kenji; Koyama, Yoshikazu; Yoshida, Kazuhiko; Kase, Satoru; Ohno, Shigeaki
Aims: Hydrogen peroxide (H2O2) is the major oxidant involved in cataract formation. Lens epithelial cells have been suggested to be the first site of oxidative damage. The authors investigated the relationship between H2O2-induced cytotoxicity and activation of nuclear factor kappa B (NF-B) in human lens epithelial (HLE) cells.
Methods: HLE B-3 cells were stimulated by various concentrations of H2O2 in the presence or absence of pyrrolidine dithiocarbamate (PDTC), a potent inhibitor of NF-B. H2O2-induced cytotoxicity was measured by lactate dehydrogenase cytotoxicity assay. Translocation of NF-B was examined by Western blot and immunocytochemistry using anti-p65 antibody.
Results: H2O2-induced cytotoxicity increased in...
5.
Positive correlation between vitreous levels of advanced glycation end products and vascular endothelial growth factor in patients with diabetic retinopathy sufficiently treated with photocoagulation - Yokoi, Masahiko; Yamagishi, Sho-ichi; Takeuchi, Masayoshi; Matsui, Takanori; Yoshida, Yumiko; Ohgami, Kazuhiro; Amano-Okamoto, Tamami; Ohno, Shigeaki
We investigated whether vitreous levels of advanced glycation end products (AGEs) were positively correlated with vascular endothelial growth factor (VEGF) in patients with diabetic retinopathy patients sufficiently treated with retinal photocoagulation. Vitreous AGE and VEGF levels were significantly higher in patients with diabetes than in controls. Positive correlation between AGE and VEGF was found in patients with diabetic retinopathy sufficiently treated with retinal photocoagulation (r = 0.44, p<0.05), but not in those who were insufficiently treated (r = 0.26, p = 0.18). The present observations suggest that AGE may induce VEGF expression in an ischaemia-independent mechanism. AGE could be one...
6.
Transient subacute cerebellar ataxia in a patient with Lambert-Eaton myasthenic syndrome after intracranial aneurysm surgery - Nakamura, Masakazu; Yabe, Ichiro; Sato, Kazunori; Nakano, Fumihito; Yaguchi, Hiroaki; Tsuji, Sachiko; Shiraishi, Hirokazu; Yoneda, Makoto; Tanaka, Keiko; Motomura, Masakatsu; Sasaki, Hidenao
Several reports have presented patients with subacute cerebellar ataxia (subacute CA) and Lambert-Eaton myasthenic syndrome (LEMS). Some clinical features of those patients have been described in the previous reports, such as priority of subacute CA to LEMS or a concurrent occurrence of both diseases, a high incidence of malignancy, less efficacy of the treatment for subacute CA compared with that for LEMS. Cerebellar ataxia in some patients with LEMS has been demonstrated to be caused by serum antibodies to P/Q-type voltage-gated calcium channels (VGCCs). Here, we report a 63-year-old woman with subacute CA and LEMS. Cerebellar ataxia appeared 15 months...
7.
Tyrosinase gene family and Vogt-Koyanagi-Harada disease in Japanese patients. - Horie, Yukihiro; Takemoto, Yuko; Miyazaki, Akiko; Namba, Kenichi; Kase, Satoru; Yoshida, Kazuhiko; Ota, Masao; Hasumi, Yukiko; Inoko, Hidetoshi; Mizuki, Nobuhisa; Ohno, Shigeaki
PURPOSE: The aim of the present study was to examine the genetic background of Vogt-Koyanagi-Harada (VKH) disease in a Japanese population by analyzing the tyrosinase gene family (TYR, TYRP1, and dopachrome tautomerase (DCT)). METHODS: 87 VKH patients and 122 healthy controls were genotyped using seven microsatellite markers on the candidate loci. We analyzed microsatellite (MS) polymorphisms at regions within tyrosinase gene family loci. In addition, the haplotype frequencies were also estimated and statistical analysis was performed. HLA-DRB1 genotyping was performed by the PCR-restriction fragment length polymorphism (RFLP) method. RESULTS: No significant evidence for an association was found. HLA-DRB1*0405 showed a...
8.
Polymorphism of IFN-gamma gene and Vogt-Koyanagi-Harada disease. - Horie, Yukihiro; Kitaichi, Nobuyoshi; Takemoto, Yuko; Namba, Kenichi; Yoshida, Kazuhiko; Hirose, Shigeto; Hasumi, Yukiko; Ota, Masao; Inoko, Hidetoshi; Mizuki, Nobuhisa; Ohno, Shigeaki
PURPOSE: Interferon-gamma (IFN-gamma) is a key cytokine in inflammatory disorders. Elevated aqueous and serum levels of IFN-gamma levels have been reported to be elevated in patients with Vogt-Koyanagi-Harada (VKH) disease. The aim of this study was to determine the IFN-gamma gene polymorphisms in VKH disease. METHODS: The study involved 136 VKH patients and 176 healthy controls, who were genotyped for functional single nucleotide polymorphism (SNP; rs2430561; A/T) and functional microsatellite (CA) repeats (rs3138557) in the first intron of the IFN-gamma gene. Moreover, clinical manifestations of the patients were also analyzed. RESULTS: Diffuse choroiditis/staining of fluorescein angiography was seen in all...
9.
The effect of antagonism of adenosine A1 receptor against ischemia and reperfusion injury of the liver - Magata, Shinichiro; Taniguchi, Masahiko; Suzuki, Tomomi; Shimamura, Tsuyoshi; Fukai, Moto; Furukawa, Hiroyuki; Fujita, Miri; Todo, Satoru
Background: Adenosine is known to exert protective roles in hepatic ischemia and reperfusion injury, while all adenosine receptors do not play the cytoprotective roles. We have tested our hypothesis that blockage of adenosine binding to A1 receptor by its antagonist, KW3902 [8-(noradamantan-3-yl)-1,3-dipropylxanthine] attenuates hepatic ischemia-reperfusion injury. Methods: Adult female beagle dogs underwent a 2 h total hepatic vascular exclusion (THVE) with a venovenous bypass. Nontreated animals that underwent THVE with a venovenous bypass alone were used as the control (Group CT, n = 6). KW3902 was given to the animals by continuous intraportal infusion for 60 min before ischemia at...
10.
Establishment of a newly improved detection system for NF-κB activity - Matsuda, Mayuko; Tsukiyama, Tadasuke; Bohgaki, Miyuki; Nonomura, Katsuya; Hatakeyama, Shigetsugu
The transcription factor nuclear factor-κB (NF-κB) plays roles in apoptosis, inflammation and oncogenesis. It is important for biological and medical research to understand when proteins of interest are activated in cells, leading to the establishment of a luciferase/EGFP assay to monitor the activation of transcription factors. Here, we describe an improved reporter system for NF-κB, the NF-κB-activated transgene (NAT) system that can detect NF-κB signalling with high sensitivity and specificity. The NAT system consists of large copy numbers of NF-κB consensus sequence and a minimal promoter derived from the mouse interleukin-2 (IL-2) gene. Furthermore, we generated NAT systems with stable...
11.
Ligand-dependent transcription of estrogen receptor α is mediated by the ubiquitin ligase EFP - Nakajima, Ayako; Maruyama, Satoru; Bohgaki, Miyuki; Miyajima, Naoto; Tsukiyama, Tadasuke; Sakuragi, Noriaki; Hatakeyama, Shigetsugu
Estrogen-mediated ubiquitylation and subsequent degradation of the estrogen receptor α (ERα) appears to be involved in the transcriptional activity of ERα. We show that the estrogen-responsive finger protein (EFP) interacts with and ubiquitylates ERα. EFP promoted the ubiquitylation of ERα in vitro and in vivo and consequently promoted the degradation of ERα. The interaction between EFP and ERα was greatly enhanced in the presence of estrogen. The action of EFP on ERα in the presence of estrogen resulted in a robust interaction between ERα and Tip60, one of the transcriptional coactivators, leading to activation of ERα transcriptional activity. However, a...
12.
X-linked Charcot-Marie-Tooth disease (CMTX) in a severely affected female patient with scattered lesions in cerebral white matter - Basri, Rehana; Yabe, Ichiro; Soma, Hiroyuki; Matsushima, Masaaki; Tsuji, Sachiko; Sasaki, Hidenao
Charcot-Marie-Tooth neuropathy (CMT) is an inherited degenerative disorder of the peripheral nervous system that results in slowly progressive distal muscle weakness, atrophy and loss of proprioception in the affected areas. X-linked CMT (CMTX) has been localized to the pericentric region of the X chromosome. CMTX neuropathy is usually associated with mutations in exon 2 of the gap junction protein β1 (GJB1) gene. GJB1 is a gap junction protein expressed in various cells including oligodendrocytes, astrocytes and myelinating schwann cells. Here, we report a female case of CMTX with a GJB1 mutation. The patient was severely clinically affected and exhibited both...
13.
Novel SCN5A mutation (Q55X) associated with age-dependent expression of Brugada syndrome presenting as neurally mediated syncope. - Makita, Naomasa; Sumitomo, Naokata; Watanabe, Ichiro; Tsutsui, Hiroyuki
Background: An association between Brugada syndrome and neurally mediated syncope has been described. Although mutations in SCN5A have been identified in Brugada syndrome, the genetic link between Brugada syndrome and neurally mediated syncope has not been determined. Objectives: The purpose of the study was to clinically and genetically characterize a man with recurrent syncope that originally was diagnosed as neurally mediated syncope at age 8 years but subsequently manifested as Brugada syndrome at age 17 years. Methods: The proband underwent clinical examination, which included head-up tilt test, sodium channel provocation test, and electrophysiologic study. Genetic screening of SCN5A was performed...
14.
Separate oscillating cell groups in mouse suprachiasmatic nucleus couple photoperiodically to the onset and end of daily activity. - Inagaki, Natsuko; Honma, Sato; Ono, Daisuke; Tanahashi, Yusuke; Honma, Ken-ichi
The pattern of circadian behavioral rhythms is photoperiod-dependent, highlighted by the conservation of a phase relation between the behavioral rhythm and photoperiod. A model of two separate, but mutually coupled, circadian oscillators has been proposed to explain photoperiodic responses of behavioral rhythm in nocturnal rodents: an evening oscillator, which drives the activity onset and entrains to dusk, and a morning oscillator, which drives the end of activity and entrains to dawn. Continuous measurement of circadian rhythms in clock gene Per1 expression by a bioluminescence reporter enabled us to identify the separate oscillating cell groups in the mouse suprachiasmatic nucleus (SCN),...
15.
Relations of occupational stress to occupational class in Japanese civil servants : analysis by two occupational stress models - Kawaharada, Mariko; Saijo, Yasuaki; Yoshioka, Eiji; Sato, Tetsuro; Sato, Hirokazu; Kishi, Reiko
The aim of the present study was to identify relations between occupational stress and occupational class in Japanese civil servants, using two occupational stress models – the Effort-Reward Imbalance (ERI) Model and the Job Demand-Control (JDC) Model. The subjects were employees of three local public organizations. We distributed self-administered questionnaires and assessed occupational stress by ERI and JDC. We used seven occupational categories based on the Standard Occupational Classification for Japan. The data of 6,423 male and 1,606 female subjects were analyzed by logistic regression analysis to obtain odds ratios (OR) for relations between occupational stress and occupational class. In...
16.
Segmental and complementary expression of L-serine biosynthetic enzyme 3-phosphoglycerate dehydrogenase and neutral amino acid transporter ASCT1 in the mouse kidney. - Takasaki, Chihiro; Miura, Eriko; Watanabe, Masahiko
3-Phosphoglycerate dehydrogenase (Phgdh) is the initial step enzyme in the phosphorylated pathway of L-serine biosynthesis. We have previously revealed in the brain that Phgdh is preferentially expressed in glial cells, but not in neurons, and that glia-borne L-serine exerts strong neurotrophic actions to neuronal survive, differentiation, and development. To investigate whether such an L-serine-meditated intercellular relationship is constructed in peripheral organs and tissues, we examined the kidney, which is one of the organs with the highest expression of Phgdh mRNA in the body. We found that Phgdh was distributed highly in the renal papilla and inner layer of the outer...
17.
Electron swarm parameters in water vapour - Hasegawa, H.; Date, H.; Shimozuma, M.
Electron swarm parameters, such as the drift velocity and the ionization coefficient, in water vapour have been measured for relatively wide ranges in reduced electric fields (E/N) at room temperature. The drift velocity (Wm) was obtained based upon the arrival-time spectra of electrons by using a double-shutter drift tube for the E/N from 60 to 1000 Td, while the first and second ionization coefficients (α and γ) were determined by the steady-state Townsend method from 50 to 3000 Td. A comparison between the results and other data in the literature shows that our results for both the drift velocity and...
18.
Effect of eradication of Helicobacter pylori on incidence of metachronous gastric carcinoma after endoscopic resection of early gastric cancer : an open-label, randomised controlled trial - Fukase, Kazutoshi; Kato, Mototsugu; Kikuchi, Shogo; Inoue, Kazuhiko; Uemura, Naomi; Okamoto, Shiro; Terao, Shuichi; Amagai, Kenji; Hayashi, Shunji; Asaka, Masahiro
Background: Although the relationship between H.pylori infection and gastric cancer has been proved in epidemiological studies and animal experiments, prophylactic effect of H. pylori eradication is controversial in human studies. Methods: We conducted a large-scale, multi-center, randomized controlled open-labeled study to determine whether eradication of H. pylori had inhibitory effects on the development of metachronous gastric carcinomas after endoscopic resection. The study included 542 subjects including patients diagnosed with early gastric cancer, either newly diagnosed and planning to have endoscopic treatment; or in the post-resection follow-up phase after endoscopic treatment. They were randomly allocated to eradication and control arms and...
19.
Endothelin-1 decreases [Ca2+]i via Na+/Ca2+ exchanger in CHO cells stably expressing endothelin ETA receptor. - Horinouchi, Takahiro; Nishimoto, Arata; Nishiya, Tadashi; Lu, Lingyun; Kajita, Emi; Miwa, Soichi
Endothelin ETA receptor couples to Gq/11 protein that transduces a variety of receptor signals to modulate diverse cellular responses including Ca2+ mobilization. Stimulation of endothelin ETA receptor with endothelin-1 is generally believed to induce an increase in intracellular Ca2+ concentration ([Ca2+]i) via Gq/11 protein. Here we provide the first convincing evidence that endothelin-1 elicited Gq/11 protein-dependent and -independent ‘decrease’ in [Ca2+]i via Na+/Ca2+ exchanger (NCX) in Chinese hamster ovary (CHO) cells stably expressing human endothelin ETA receptor. In the cells treated with 1 μM thapsigargin, an inhibitor of endoplasmic Ca2+ pump, that induces an increase in [Ca2+]i via capacitative Ca2+...
20.
OCTN2VT, a splice variant of OCTN2, does not transport carnitine because of the retention in the endoplasmic reticulum caused by insertion of 24 amino acids in the first extracellular loop of OCTN2 - Maekawa, Satoshi; Mori, Daisuke; Nishiya, Tadashi; Takikawa, Osamu; Horinouchi, Takahiro; Nishimoto, Arata; Kajita, Emi; Miwa, Soichi
A novel organic cation transporter OCTN2 is indispensable for carnitine transport across plasma membrane and subsequent fatty acid metabolism in the mitochondria. Here, we report a novel splice variant of OCTN2 (OCTN2VT), in which a 72-base-pair sequence located in the first intron of OCTN2 gene was spliced between exons 1 and 2 of OCTN2, causing the insertion of 24 amino acids in the first extracellular loop of OCTN2. Despite the similarity between OCTN2 and OCTN2VT regarding primary structure and tissue distribution, their biochemical characteristics were significantly different. OCTN2 was expressed on the plasma membrane with robust N-glycosylation, whereas OCTN2VT was...
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