Hokkaido University Collection of Scholarly and Academic Papers
(65.351 recursos)
HUSCAP (Hokkaido University Collection of Scholarly and Academic Papers) contains peer-reviewed journal articles, proceedings, educational resources and any kind of scholarly works of Hokkaido University.
Mostrando recursos 1 - 20 de 70
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X-ray Absorption Fine Structure (XAFS) Analysis of Titanium-implanted Soft Tissue - Uo, Motohiro; Asakura, Kiyotaka; Yokoyama, Atsuro; Ishikawa, Makoto; Tamura, Kazuchika; Totsuka, Yasunori; Akasaka, Tsukasa; Watari, Fumio
Tissues contacting Ti dental implants were subjected to X-ray absorption fine structure (XAFS) analysis to examine the chemical state of Ti transferred from the placed implant into the surrounding tissue. Nine tissues that contacted pure Ti cover screws for several months were excised in a second surgery whereby healing abutments were set. Six tissues that surrounded implants retrieved due to their failure were also excised. Ti distributions in the excised specimens were confirmed by X-ray scanning analytical microscopy (XSAM), and the specimens were subjected to fluorescence XAFS analysis to determine the chemical states of the low concentrations of Ti in...
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The innermost layer of cementum in rat molars: its ultrastructure, development, and calcification. - Yamamoto, T.
The present study describes the ultrastructure of the innermost layer of cementum (ILC) in the rat molars and discusses its developmental process and calcification mechanisms. The following points are the main results of this study:
1. The ILC is a thin layer, about 2.0μm thick, intensely stained with hematoxylin but not stained by silver impregnation.
2. Electron microscopically, it is composed of substances stained with ruthenium red and chromic phosphotungstic acid presumed to be proteoglycans and a few thin collagen fibrils.
3. Periodontal fibers penetrate the ILC only a short distance, and do not reach the root dentin surface.
4. The ILC begins to...
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Recovery of dynamic balance after additional small divided doses of midazolam given intravenously for sedation. - Fujisawa, Toshiaki; Iida, Akira; Takuma, Shigeru; Koseki, Hiroyo; Kimura, Kunie; Fukushima, Kazuaki
We have previously reported that a dynamic balance test with perturbation stimuli and computerised dynamic posturography sensitively reflected the inhibitory effect on balance of intravenous midazolam sedation given intravenously as a single dose, and recovery time was within 80 min. The purpose of this study was to investigate the recovery of dynamic balance after additional doses of midazolam.
Eighteen young adult male volunteers were sedated with midazolam given intravenously. The initial dose was given until the Wilson sedation score reached 3, and an additional dose was given until the same score was obtained 40 min later. They were tested with perturbation...
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Participation of the Fas and Fas ligand systems in apoptosis during atrophy of the rat submandibular glands - Takahashi, Shigeru; Gobe, Glenda C.; Yoshimura, Yoshitaka; Kohgo, Takao; Yamamoto, Tsuneyuki; Wakita, Minoru
Most acinar cells and some duct cells undergo apoptosis during atrophy of the submandibular gland. The present study was designed to elucidate whether Fas and its receptor ligand (FasL) are involved during apoptotic atrophy of the gland. The excretory duct of the right submandibular gland of rats was doubly ligated with metal clips from 1 to 14 days for induction of gland atrophy. Control rats were untreated. Fas and FasL expression in the atrophied submandibular gland was detected using immunohistochemistry (IHC) and Western immunoblot. Expression of activated caspase 8 and activated caspase 3 was also detected with IHC. Fas-positive acinar...
5.
Correlation between precise and simple tests in recovery of dynamic balance function after intravenous sedation with midazolam in the elderly. - Fujisawa, Toshiaki; Takuma, Shigeru; Koseki, Hiroyo; Kimura, Kunie; Fukushima, Kazuaki
Purpose Assessing the recovery of dynamic balance after intravenous sedation in the elderly is important for ensuring their safe discharge, especially when they are walking. A reliable, simple dynamic balance test would be useful in daily clinical practice. We observed the recovery of balance after intravenous sedation with midazolam, using computerized dynamic posturography (CDP), and we evaluated the correlation between the CDP result and the results of simple dynamic balance tests.
Methods Midazolam was administered in divided doses, until the Wilson sedation score reached 3, in 18 elderly male volunteers. The dynamic balance test using CDP with perturbation stimuli was...
6.
Gene silencing by the tRNA maturase tRNase ZL under the direction of small-guide RNA. - Nakashima, A.; Takaku, H.; Shibata, H.; Negishi, Y.; Takagi, M.; Tamura, M.; Nashimoto, M.
We have been developing a unique system for the downregulation of a gene expression through cutting a specific mRNA by the long form of tRNA 3'-processing endoribonuclease (tRNase ZL) under the direction of small-guide RNA (sgRNA). However, the efficacy of this system and the involvement of tRNase ZL in the living cells were not clear. Here we show, by targeting the exogenous luciferase gene, that the efficacy of the sgRNA/tRNase ZL method can become comparable to that of the RNA interference technology and that the gene silencing is owing to tRNase ZL directed by sgRNA not owing to a simple...
7.
Study on the usefulness of precise and simple dynamic balance tests for the evaluation of recovery from intravenous sedation with midazolam and propofol. - Fujisawa, T.; Takuma, S.; Koseki, H.; Kimura, K.; Fukushima, K.
Background and objective: Dynamic balance involving movement of the centre of gravity is important for the evaluation of street fitness after sedation. The purpose of this study was to compare the recovery of dynamic balance after intravenous sedation with propofol or midazolam, and to investigate the usefulness of simple dynamic balance tests in evaluating the recovery. Methods: Fourteen young male volunteers underwent intravenous sedation with propofol and midazolam for 1 h each at an interval of more than 1 week. Computerized dynamic posturography using a multi-axial tilting platform, the 10-m maximum-speed walking test and the timed ‘up & go’ test...
8.
Recovery from Postsurgical Swallowing Dysfunction in Patients With Oral Cancer - Tei, Kanchu; Maekawa, Kuniaki; Kitada, Hideaki; Ohiro, Yoichi; Yamazaki, Yutaka; Totsuka, Yasunori
Patients and Methods: The swallowing function was assessed in 25 patients (18 males and 7 females) with oral cancer. The swallowing function was assessed preoperatively and 1, 6, and 12 months and in some cases more than 24 months after surgery. Assessment of the swallowing function consisted of videofluoroscopic evaluation (oropharyngeal swallow efficiency; OPSE) and details of the method of nutrition, diet level, nutritional condition, and occurrence of pneumonia. Results: Postsurgically, OPSE did not recover to the preoperative level more than 12 months after surgery. Twenty-one patients (84%) had full oral intake and only 3 patients (12%) showed poorer nutritional...
15.
Establishment of cell lines that exhibit pluripotency from miniature swine periodontal ligaments. - Ibi, Miho; Ishisaki, Akira; Yamamoto, Matsuo; Wada, Satoshi; Kozakai, Takaharu; Nakashima, Aiko; Iida, Jynichiro; Takao, Sonshin; Izumi, Yuichi; Yokoyama, Atsuro; Tamura, Masato
Objective: The periodontal ligament (PDL) is a fibrous connective tissue composed of heterogeneous cell types, including PDL fibroblasts. It is not clear whether cells within the PDL fibroblast population retain the potency to differentiate into other cell types. Design: In the present study, clonal cell lines, derived from Clawn miniature swine PDLs, were established by gene transfection for a human telomerase reverse transcriptase, and characterized. Results: These cell lines, denoted TesPDL1–4, had PDL fibroblasts that showed fibroblastic morphology and expressed procollagen α1(I), osteopontin, periostin and alkaline phosphatase mRNA. Under the specific culture conditions, TesPDL3 cells also have the ability to...
17.
Quantitative method for measurement of aerotolerance of bacteria and its application to oral indigenous anaerobes. - KIKUCHI, HIROKO E.; SUZUKI, TAKESHI
An index which expressed the aerobic to anaerobic potential was made for bacteria with intermediate tolerance for oxygen. One method used for this analysis was measurement of the relative bacterial growth ratio. The other method was based on the pattern of the absorbancy versus depth plot. The index was applied to oral indigenous anaerobes.
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