Hokkaido University Collection of Scholarly and Academic Papers
(65.390 recursos)
HUSCAP (Hokkaido University Collection of Scholarly and Academic Papers) contains peer-reviewed journal articles, proceedings, educational resources and any kind of scholarly works of Hokkaido University.
Mostrando recursos 1 - 20 de 29
1.
Cross-talk between Wnt and bone morphogenetic protein (BMP)- 2 signalling in differentiation pathway of C2C12 myoblasts - Nakashima, Aiko; Katagiri, Takanobu; Tamura, Masato
Loss of function of the Wnt co-receptor, lipoprotein receptor-related protein 5, decreases bone formation, and a point mutation in this gene results in high bone mass, indicating the importance of this signalling pathway in bone formation. However, the exact mechanism is currently unknown. We examined a potential role for Wnt signalling and functional cross-talk of bone morphogenetic protein (BMP)-2 in osteoblast differentiation. To assess the contribution of Wnt, we generated C2C12 cells overexpressing Wnt3a or Wnt5a and treated these with BMP-2. We showed that expression of matix extracellular phosphoglycoprotein was induced by BMP-2 in Wnt3a over-expressing C2C12 cells but not...
2.
Regulation of matrix metalloproteinase-13 and tissue inhibitor of matrix metalloproteinase-1 gene expression by WNT3A and bone morphogenetic protein-2 in osteoblastic differentiation - Aiko, Nakashima; Masato, Tamura
During bone remodeling, degradation of skeletal connective tissue is regulated, at least in part, by the balance between matrix metalloproteinases (MMPs) and tissue inhibitors of matrix metalloproteinase (TIMPs), their natural inhibitors. Recently, the Wnt signaling pathway has been demonstrated to play a crucial role in the regulation of bone formation. Here, we investigated a potential role for Wnt signaling and functional cross-talk with bone morphogenetic protein (BMP)-2 in mRNA expression of MMPs, TIMPs and bone matrix proteins in pluripotent C2C12 cells. To assess the functional contribution of Wnt signaling, we have generated C2C12 cell lines stably over-expressing Wnt3a or Wnt5a,...
3.
Extracellular inorganic phosphate regulates gibbon ape leukemia virus receptor-2/phosphate transporter mRNA expression in rat bone marrow stromal cells - Wada, Keinoshin; Mizuno, Morimichi; Komori, Takahide; Tamura, Masato
In mammalian cells, several observations indicate not only that phosphate transport probably regulates local inorganic phosphate (Pi) concentration, but also that Pi affects normal cellular metabolism, which in turn regulates apoptosis and the process of mineralization. To elucidate how extracellular Pi regulates cellular functions of pre-osteoblastic cells, we investigated the expression of type III sodium (Na)-dependent Pi transporters in rat bone marrow stromal cells and ROB-C26 pre-osteoblastic cells. The mRNA expression level of gibbon ape leukemia virus receptor (Glvr)-2 was increased by the addition of Pi in rat bone marrow stromal cells, but not in ROB-C26 or normal rat kidney...
4.
Periodontal ligament cells under intermittent tensile stress regulate mRNA expression of osteoprotegerin and tissue inhibitor of matrix metalloprotease-1 and -2 - Tsuji, Kiyomi; Uno, Keiji; Zhang, Gui Xia; Tamura, Masato
We studied the mRNA expression of osteoprotegerin (OPG), receptor activator of NF-kappa B ligand (RANKL), tissue inhibitor of matrix metalloprotease (TIMP)-1 and -2, and matrix metalloprotease (MMP)-1 and -2 by human periodontal ligament (PDL) cells under intermittent tensile stress using a Flexercell Strain Unit. Analysis by reverse transcriptase-polymerase chain reaction showed that mechanical force upregulated OPG mRNA. We also demonstrated that the protein concentration of OPG in conditioned medium increased upon loading with tensile stress, as determined by enzyme-linked immunosorbent assay. TIMP-1 and -2 mRNA levels also increased, whereas levels of RANKL, MMP-1, and MMP-2 mRNA were barely affected. We...
5.
Regulation of mRNA Expression of Matrix Extracellular Phosphoglycoprotein (MEPE)/ Osteoblast/Osteocyte Factor 45 (OF45) by Fibroblast Growth Factor 2 in Cultures of Rat Bone Marrow-derived Osteoblastic Cells - Zhang, Gui Xia; Mizuno, Morimichi; Tsuji, Kiyomi; Tamura, Masato
Matrix extracellular phosphoglycoprotein (MEPE)/ osteoblast/osteocyte factor 45 (OF45) is a recently isolated RGD-containing matrix protein that acts as the tumor-derived phosphaturic factor in oncogenic hypophosphatemic osteomalacia. It is also highly expressed by osteoblasts and osteocytes. We examined the regulation of MEPE/OF45 mRNA expression in osteoblastic cells derived from high-density cultures of primary rat bone marrow stromal cells incubated with dexamethasone, β-glycerophosphate, and ascorbic acid. The level of MEPE/OF45 mRNA in these cells was down-regulated by the addition of fibroblast growth factor 2 (FGF2) for 48 h. These effects were observed in a dose-dependent manner between 2 and 10 ng/mL. FGF2...
6.
RECOVERY OF INTENTIONAL DYNAMIC BALANCE FUNCTION AFTER INTRAVENOUS SEDATION WITH MIDAZOLAM IN YOUNG AND ELDERLY SUBJECTS - Fujisawa, Toshiaki; Takuma, Shigeru; Koseki, Hiroyo; Kimura, Kunie; Fukushima, Kazuaki
Background and objective: Computerized dynamic posturography using an intentional postural sway task can be used to assess body-leaning ability and postural control ability to prevent falls. Falls are the leading cause of morbidity and mortality for the elderly. The purpose of the present study was to evaluate the recovery of intentional dynamic balance function after intravenous sedation with midazolam in elderly subjects in comparison with that in young subjects.
Methods: Midazolam was administered in small, divided doses over 4 to 5 minutes until the Wilson sedation score reached 3 in 20 young and 18 elderly male volunteers. A dynamic limits of...
7.
Influence of intravenous sedation with midazolam on Respiratory function and muscle activity inelderly and young patients - Fujisawa, Toshiaki; Suzuki, Shinobu; Kurozumi, Akihiro; Kimura, Yukifumi; Iida, Akira; Fukushima, Kazuaki
Objective: The purpose of this study was to investigate the optimal dosage of intravenous midazolam for sedation in elderly patients and compare the amount of depression of respiratory function and skeletal muscle activity after obtaining similar sedation levels in younger patients.
Patients and Methods: Fifteen elderly patients older than 65 years (group E) and 15 patients younger than 55 years (group Y) underwent oral surgery or dental treatment with or without local anaesthesia after intravenous administration of midazolam.
Results: The optimal dose for the elderly patients was 62% that for the younger patients. The duration of depression of arterial oxygen saturation and...
8.
Apatite formation on carbon nanotubes - Akasaka, Tsukasa; Watari, Fumio; Sato, Yoshinori; Tohji, Kazuyuki
Apatite coating on carbon nanotubes (CNTs) was done with a biomimetic coating method. The multi-walled CNTs (MWNTs) of curled shape with about 30 nm in diameter were immersed for 2 weeks in the simulated body fluid. Observation by scanning electron microscopy (SEM) showed the formation of apatite on the MWNTs surface. The clusters of spherules consisting of needle shape of apatite crystallites were massively grown on the aggregated MWNTs. The crystallites of 100 nm in width and 200-500 nm in length were grown perpendicularly to the longitudinal direction and radially originating from a common center of a single MWNT. Thus...
9.
Staining of hybrid composites with coffee, oolong tea, or red wine - Omata, Yo; Uno, Shigeru; Nakaoki, Yasuko; Tanaka, Toru; Sano, Hidehiko; Yoshida, Shigemitsu; Sidhu, Sharanbirk
This study examined the surface staining mechanism of a photopolymerized composite by coffee, oolong tea, and red wine.
Dental composite was subjected to an experimental 24-hour staining cycle: 17-hour immersion in artificial saliva solution containing 0.3% mucin followed by 7-hour immersion in coffee, tea, or wine. After one, two, and four weeks, digital images of the composite surface were analyzed in grayscale mode with an imaging analyzer. Specimens polished but not immersed were used as a baseline measurement for color change. Additionally, the effects of mechanical brushing and chlorhexidine on drink-induced staining were examined.
Wine caused the most severe staining, followed by...
10.
Effect of enamel shades on color of layered resin composites - Kamishima, Naoko; Ikeda, Takatsumi; Sano, Hidehiko
The purpose of this study was to evaluate the effect of the covering enamel layer of various thicknesses on the color of layered resin composites (LRC). To this end, the CIELAB parameters of the following were evaluated: underlying base (ULB: 2.0 mm of body shade + 3.0 mm of opaque shade), inherent color of the enamel shade (4.0 mm of enamel shade + ULB), and LRC disks (0.5, 1.0, 2.0, 3.0 mm of enamel shade + ULB) of Filtek Supreme (FS, 3M) and Gradia Direct (GD, GC). To assess the effect of the enamel layer on the color of LRC,...
11.
Sublethal concentrations of diverse gold compounds inhibit mammalian cytosolic thioredoxin reductase (TrxR1) - Omata, Yo; Folan, Matt; Shaw, Melissa; Messer, Regina L.; Lockwood, Petra E.; Hobbs, David; Bouillaguet, Serge; Sano, Hidehiko; Lewis, Jill B.; Wataha, John C.
Thioredoxin reductase (TrxR) reduces thioredoxin (Trx), thereby contributing to cellular redox balance, facilitating the synthesis of deoxy-ribose sugars for DNA synthesis, and regulating redox-sensitive gene expression. Auranofin is a gold compound that potently inhibits TrxR. This inhibition is one suspected mechanism of auranofin’s therapeutic benefit in the treatment of rheumatoid arthritis. The use of other gold compounds to treat cancer or inflammatory disease may rely on their ability to inhibit TrxR. In the current study, we tested the hypothesis that a variety of gold compounds may inhibit TrxR.
Methods: We exposed rat-TrxR1 to auranofin, gold sodium thiomalate, sodium aurothiosulfate, triphenyl phosphine...
12.
Intra- and extracellular reactive oxygen species (ROS) generated by blue light - Omata, Y.; Lewis, J.B.; Rotenberg, S.; Lockwood, P.E.; Messer, R.L.W.; Noda, M; Hsu, S.D.; Sano, H.; Wataha, J.C.
The title when publishing in the magazine [Intra- and extracellular reactive oxygen species generated by blue light]
14.
Relationship between stress factor and periodontal disease in a rural area population in Japan - Akhter, Rahena; Hannan, MA; Okhubo, R; Morita, M
Objectives: Several studies conducted in Western countries have shown significant associations between stress factors and periodontal disease. However, there have been only a few studies conducted in Asian countries. The present study was designed to identify possible relationship between stress and periodontal disease in residents of a rural area in Japan.
Material and Methods: Data were collected from 1,089 adults with at least six natural teeth in a typical farming district of Japan. Subjects were asked to complete a questionnaire on daily stresses in various aspects of life. Data on gender, occupation, smoking, alcohol drinking habits, dental health behavior and...
15.
Biological behavior of myoepithelial cells in the regeneration of rat atrophied sublingual glands following release from duct ligation - Takahashi, S.; Kohgo, T.; Nakamura, S.; Arambawatta, A. K. S.; Domon, T.; Wakita, M.
The present study aimed to clarify how myoepithelial cells behave during regeneration of an atrophied sublingual gland by investigating cell proliferation and ultrastructure. Atrophy of rat sublingual glands was induced by unilateral ligation of the excretory duct near the hilum with metal clips, which were then removed after one week of ligation for regeneration. The sublingual glands 0–14 days after unligation were examined with single immunohistochemistry for actin as a marker of myoepithelial cells, double immunohistochemistry for actin and proliferating cell nuclear antigen (PCNA) as a marker of proliferating cells, and transmission electron microscopy (TEM). The single immunohistochemistry and TEM...
16.
Relationships between lifestyle and dental health behaviors in a rural population in Japan - Harada, Shoji; Akhter, Rahena; Kurita, Keiko; Mori, Miyako; Hoshikoshi, Misuzu; Tamashiro, Hidehiko; Morita, Manabu
The aim of this study was to determine associations of
lifestyle with dental health behaviors such as tooth brushing frequency, use of
extra cleaning devices, and regular dental visits to a dentist. Methods: Data
were collected from 1182 dentate residents 18 years of age or older who resided
in a typical farming district. The data included data on the demographic factors,
dental health behavior, and various aspects of lifestyle, i.e. mental condition,
alcohol consumption, smoking habit, physical activity, social activity, dietary
habits, and presence of systemic diseases. Results: Multiple logistic regression
analysis revealed that subjects in a younger group (18–39 years of age) and
subjects who had never smoked...
17.
Continuous assay of protein tyrosine phosphatases based on fluorescence resonance energy transfer - Nishikata, M; Yoshimura, Y; Deyama, Y; Suzuki, K
An assay method that continuously measures the protein tyrosine phosphatase
(PTP)-catalyzed dephosphorylation reaction based on fluorescence resonance energy
transfer (FRET) was developed as an improvement of our previously reported
discontinuous version [M. Nishikata, K. Suzuki, Y. Yoshimura, Y. Deyama, A.
Matsumoto, Biochem. J. 343 (1999) 385-391]. The assay uses oligopeptide substrates
that contain Mca [(7-methoxycoumarin-4-yl)acetyl] group as a fluorescence donor and
DNP (2,4-dinitrophenyl) group as a fluorescence acceptor, in addition to a
phosphotyrosine residue located between these two groups. In the assay, a PTP solution
is added to a buffer solution containing a FRET substrate and chymotrypsin. The
PTP-catalyzed dephosphorylation of the substrate and subsequent chymotryptic
cleavage of the dephosphorylated...
18.
Effect of surface condition of dental zirconia ceramic (Denzir) on bonding - Uo, Motohiro; Sjögren, Göran; Sundh, Anders; Goto, Mitsunari; Watari, Fumio; Bergman, Maud
Yttria partially stabilized zirconia (YPSZ) ceramics are suitable for dental and medical use because of their high fracture toughness and chemical durability. The purpose of this study was to examine the bonding behavior of a dental YPSZ ceramic, Denzir. After being subjected to various surface treatments, Denzir specimens were bonded to each other using an adhesive resin composite, glass ionomer, or zinc phosphate cement. Bonding strength was then determined by the shearing test. No significant differences (p>0.05) were observed between SiC- and Al2O3-blasted specimens. In all surface treatments, the shear bond strength significantly (p<0.05) increased in the order of adhesive...
19.
The effect of the air-blowing step on the technique sensitivity of four different adhesive systems - Spreafico, Diego; Semeraro, Stefano; Mezzanzanica, Dario; Re, Dino; Gagliani, Massimo; Tanaka, Toru; Sano, Hidehiko; Sidhu, Sharanbir K.
Purpose:
To evaluate the technique sensitivity of four different adhesive systems using different air-blowing pressure.
Methods:
Four adhesive systems were employed: Clearfil SE Bond [SE] (Kuraray, Japan), G-Bond [GB] (GC Corporation, Japan), Adper Prompt L-Pop [LP] (3M ESPE, USA) and an experimental adhesive, SSB-200 [SSB] (Kuraray, Japan). Twenty-four extracted molars were used. After grinding the coronal enamel surface, the teeth were divided into two equal groups. The first group's teeth were randomly assigned for bonding with the different adhesives using gentle air-blowing (g). For the teeth of the second group, the four adhesive systems were applied using strong air-blowing (s). After storage overnight...
20.
Rapid analysis of metallic dental restorations using X-ray scanning analytical microscopy - Uo, Motohiro; Watari, Fumio
Objectives: X-ray scanning analytical microscopy (XSAM) makes it possible to analyze small specimens in air without pretreatment. The purpose of this study was to utilize XSAM for the rapid analysis of metallic dental restorations by microsampling. Methods: Six different dental alloys were scratched with brand-new silicone points to obtain metal on the silicone point for compositional analysis. The fluorescent spectra of XSAM were measured to determine the metal attached to the specimen. Results: The major components of the six dental metals, except for palladium, were clearly detected. The identification of palladium was difficult since the fluorescent X-ray of palladium is...
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