Hokkaido University Collection of Scholarly and Academic Papers
(65.351 recursos)
HUSCAP (Hokkaido University Collection of Scholarly and Academic Papers) contains peer-reviewed journal articles, proceedings, educational resources and any kind of scholarly works of Hokkaido University.
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Evaluation of phosphorus starvation inducible bypasses to increase the effieciency of phosphorus utilization in rice - Shinano, Takuro; Nanamori, M.; Dohi, M.; Wasaki, J.; Osaki, M.
Plants develop strategies to recycle phosphorus so that all organs receive adequate amount of phosphorus, especially new growing organs. To evaluate the metabolic adaptation of rice plant under phosphorus deficient condition, we selected several genes relating phosphorus utilizing efficiency in the cell. Phosphoenolpyruvate carboxylase, triose phosphate translocator, phosphoenolpyruvate/inorganic phosphate translocator (PPT), pyruvate kinase, NAD dependent glyceraldehydes-3-phosphate dehydrogenase, NADP dependent glyceraldehydes-3-phosphate dehydrogenase, were selected because of their important role in the phosphorus utilization in the cell and 2 consisting proposed bypass pathway to save phosphate. Most dramatic change was observed in the expression level of PPT (which tranport phosphoenolpyruvate (PEP) in...
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Plant growth promotion abilities and microscale bacterial dynamics in the rhizosphere of Lupin analysed by phytate utilization ability - Unno, Yusuke; Okubo, Kenzo; Wasaki, Jun; Shinano, Takuro; Osaki, Mitsuru
In the rhizosphere, phosphorus (P) levels are low due to P uptake into the roots. Rhizobacteria live on carbon (C) exuded from roots, and may contribute to plant nutrition by liberating P from organic compounds such as phytates. We isolated over 300 phytate (Na-inositol hexa-phosphate; Na-IHP)-utilizing bacterial strains from the rhizosheath and the rhizoplane of Lupinus albus (L.). Almost all of the isolates were classified as Burkholderia based on 16S rDNA sequence analysis. Rhizosheath isolates cultured with Na-IHP as the only source of C and P showed lower P uptake at the same extracellular phytase activity than rhizoplane strains, suggesting...
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Developmental regulation of photosynthate distribution in leaves of rice - SHINANO, TAKURO; NAKAJIMA, KANTO; WASAKI, JUN; MORI, HARUHIDE; ZHENG, TEI; OSAKI, MITSURU
mRNA expression patterns of genes for metabolic key enzymes sucrose phosphate synthase (SPS), phosphoenolpyruvate carboxylase (PEPC), pyruvate kinase, ribulose 1,5-bisphosphate carboxylase/oxygenase, glutamine synthetase 1, and glutamine synthetase 2 were investigated in leaves of rice plants grown at two nitrogen (N) supplies (N0.5, N3.0). The relative gene expression patterns were similar in all leaves except for 9th leaf, in which mRNA levels were generally depressed. Though increased N supply prolonged the expression period of each mRNA, it did not affect the relative expression intensity of any mRNA in a given leaf. SPS Vmax, SPS limiting and PEPC activities, and carbon flow...
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Difference in Carbon Distribution of Initial Photoassimilates between Soybean and Rice as Revealed by 20 s Pulse-300 s Chase Experiments - Okazaki, Keiki; Shinano, Takuro; Osaki, Mitsuru
Short pulse-chase labeling experiments indicated that there was a considerable difference between soybean and rice in the distribution pattern of 14C to major metabolites during a 300 s chase period. The distribution of 14C to starch at the end of the chase period was largest in soybean but very small in rice. In rice, the distribution of 14C to sucrose was the largest. Starch formation during the initial stages of photosynthesis implied the existence of a different mechanism of starch synthesis and utilization between soybean and rice. The relative intensity of the flow into starch increased by treatment with 2%...
6.
Developmental changes of plant affecting primary photosynthate distribution in rice leaves - Shinano, T.; Ando, K.; Okazaki, K.; Osaki, M.
Developmental changes of plant in the regulation of photosynthate distribution of leaves were studied in hydroponically cultivated rice by the 14CO2 tracer technique and analysis of the activity of the regulatory enzymes, sucrose phosphate synthase (SPS), phosphoenolpyruvate carboxylase (PEPC), and pyruvate kinase (PK). The distribution of primary photosynthates into sugars, amino acids, organic acids, sugar phosphates, proteins, and polysaccharides was determined by column chromatography. The relative primary photosynthate distribution to the sugar phosphate fraction was significantly larger in the 5th leaf than in the 6th one. Correspondingly, the Vmax of PEPC was significantly higher in the 5th than in the...
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