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Hokkaido University Collection of Scholarly and Academic Papers (52.405 recursos)
HUSCAP (Hokkaido University Collection of Scholarly and Academic Papers) contains peer-reviewed journal articles, proceedings, educational resources and any kind of scholarly works of Hokkaido University.

Mostrando recursos 1 - 20 de 51

1. Expression of Foreign Genes in Lamprey Embryos: An Approach to Study Evolutionary Changes in Gene Regulation - Kusakabe, Rie; Tochinai, Shin; Kuratani, Shigeru; ??, ?
Evolution in development can be viewed as a sequence of changes in gene regulation.

2. Lamprey Contractile Protein Genes Mark Different Populations of Skeletal Muscles During Development - Kusakabe, Rie; Takechi, Masaki; Tochinai, Shin; Kuratani, Shigeru; ??, ?
Agnathan lampreys retain ancestral characteristics of vertebrates in the morphology of skeletal muscles derived from two mesodermal regions: trunk myotomes and unsegmented head mesoderm.

3. Bipolar Head Regeneration Induced by Artificial Amputationin Enchytraeus japonensis (Annelida, Oligochaeta) - Kawamoto, Shishin; Yoshida-Noro, Chikako; Tochinai, Shin; ??, ?
Normally, each of the 5–10 fragments derived from a single worm regenerates a head anteriorly and a tail posteriorly.

4. Crystal structure of the putative RNA methyltransferase PH1948 from Pyrococcus horikoshii, in complex with the copurified S-adenosyl-L-homocysteine - Gao, Yong-Gui; Yao, Min; Yong, Zhou; Tanaka, Isao; ?, ?
No abstract.

5. Elucidation of stability determinants of cold-adapted monomeric isocitrate dehydrogenase from a psychrophilic bacterium, Colwellia maris, by construction of chimeric enzymes - Watanabe, Seiya; Yasutake, Yoshiaki; Tanaka, Isao; Takada, Yasuhiro; ??, ??
The catalytic activity (kcat) and catalytic efficiency (kcat/Km) of the wild-type AvIDH and CmIDH were higher than those of EcIDH, implying that an improved catalytic rate more than compensates for the loss of a catalytic site in the former two IDHs due to monomerization.

6. Amino acid residues involved in cold adaptation of isocitrate lyase from a psychrophilic bacterium, Colwellia maris - Watanabe, Seiya; Takada, Yasuhiro; ??, ??
To investigate the mechanism of cold adaptation of isocitrate lyase (ICL; EC 4.1.3.1) from the psychrophilic bacterium Colwellia maris, Gln207 and Gln217 of this enzyme were substituted by His and Lys, respectively, by site-directed mutagenesis.

7. The cold-inducible icl gene encoding thermolabile isocitrate lyase of a psychrophilic bacterium, Colwellia marisa - Watanabe, Seiya; Yamaoka, Naoto; Takada, Yasuhiro; Fukunaga, Noriyuki; ??, ??
The gene encoding isocitrate lyase (ICL; EC 4.1.3.1) of a psychrophilic bacterium, Colwellia maris, was cloned and sequenced.

8. Gel friction: A model based on surface repulsion and adsorption - Gong, Jianping; Osada, Yoshihito; ??, ??
A model describing the frictional force produced when a polymer gel is sliding on a solid surface has been proposed from the viewpoint of solvated polymer repulsion and adsorption theory at a solid surface.

9. Structural assignment of isomeric 2-aminopyridine derivatized monosialylated biantennary N-linked oligosaccharides using negative-ion MSn spectral matching - Deguchi, Kisaburo; Takegawa, Yasuhiro; Ito, Hiroki; Miura, Nobuaki; Yoshioka, Shinji; Nagai, Shinji; Nakagawa, Hiroaki; Nishimura, Shin-Ichiro; ??, ???
To investigate the possibility of structural assignment based on negative-ion MSn spectral matching, four isomers of 2-aminopyridine-derivatized (PA) monosialylated oligosaccharides (i.e., complex type N-glycans with an ?2-3 or ?2-6 linked sialic acid on ?1-6 or ?1-3 antennae) were analyzed by using high-performance liquid chromatography/electrospray ion trap time-of-flight mass spectrometry(HPLC/ESI-IT-TOF MS).

10. Temperature adaptation in Dictyostelium: role of ?5 fatty acid desaturase - Saito, Tamao; Kato, Atsushi; Ochiai, Hiroshi; Morita, Naoki; ??, ??
Membrane fluidity is critical for proper membrane function and is regulated in part by the proportionof unsaturated fatty acids present in membrane lipids.

11. Complementary structural information of positive- and negative-ion MSn spectra of glycopeptides with neutral and sialylated N-glycans - Deguchi, Kisaburo; Ito, Hiroki; Takegawa, Yasuhiro; Shinji, Nagai; Nakagawa, Hiroaki; Nishimura, Shin-Ichiro; ??, ???
Positive- and negative-ion MSn spectra of chicken egg yolk glycopeptides binding a neutral and a sialylated N-glycan were acquired by using electrospray ionization linear ion trap time-of-flight mass spectrometry (ESI-LIT-TOFMS) and collision-induced dissociation (CID) with helium as collision gas.

12. Calcium-binding and structural stability of echidna and canine milk lysozymes - Kikuchi, M; Kawano, K; Nitta, K; ??, ??
For echidna and canine milk lysozymes, which were presumed to be the calcium-binding lysozymes by their amino acid sequences, we have quantitated their calcium-binding strength and examined their guanidine unfolding profiles.

13. Pressure-induced unfolding of lysozyme in aqueous guanidinium chloride solution - Sasahara, K; Nitta, K; ??, ??
The pressure-induced unfolding of lysozyme was investigated in an aqueous guanidinium chloride solution by means of ultraviolet spectroscopy.

14. Effect of hydrostatic pressure on unfolding of ?-lactalbumin: volumetric equivalence of the molten globule and unfolded state - Kobashigawa, Y; Sakurai, M; Nitta, K; ??, ??
The effect of pressure on the unfolding of bovine alpha-lactalbumin was investigated by ultraviolet absorption methods.

15. Up-regulation of P450arom and down-regulation of Dmrt-1 genes in the temperaturedependent sex reversal from genetic males to phenotypic females in a salamander - Sakata, Natsuko; Miyazaki, Kinuko; Wakahara, Masami
The original publication is available at http://www.springerlink.com/.

16. Single-chain tissue-type plasminogen activator is a substrate of mouse glandular kallikrein 24 - Matsui, Hitoshi; Takano, Naoharu; Moriyama, Akihiko; Takahashi, Takayuki
The present study showed that among the proteases, mK24 markedly enhanced the activity of human recombinant single-chain tissue-type plasminogen activator when the two were incubated together.

17. Enterotoxin/guanylin receptor type guanylyl cyclases in non-mammalian vertebrates - Nakauchi, Mina; Suzuki, Norio
Cyclic GMP is a ubiquitous intracellular second messenger produced by guanylyl cyclases (GCs).

18. Expression of guanylyl cyclase genes in medaka hybrids (Oryzias curvinotus x Oryzias latipes). - Shiga, T.; Suzuki, N.
The Hong Kong-originated medaka fish Oryzias curvinotus expresses nine genes (OcGC1?OcGC8 and OcGC-R2) for membrane guanylyl cyclases (membrane GCs) and three genes (OcGCS-?1, OcGCS-?2, and OcGCS-?1) for soluble GC subunits.

19. Plasma and urine levels of electrolytes, urea and steroid hormones involved in osmoregulation of cetaceans - Birukawa, Naoko; Ando, Hironori; Goto, Mutsuo; Kanda, Naohisa; Pastene, Luis A.; Nakatsuji, Hiroki; Hata, Hiroshi; Urano, Akihisa
In the present study, we determined blood and urine levels of various chemicals involved in osmoregulation, compared them with those in artiodactyls, and characterized the values in the whales.

20. A novel membrane guanylyl cyclase expressed in medaka (Oryzias latipes) intestine. - Iio, Kaori; Nakauchi, Mina; Yamagami, Sayaka; Tsutsumi, Makiko; Hori, Hiroshi; Naruse, Kiyoshi; Mitani, Hiroshi; Shima, Akihiro; Suzuki, Norio
A novel membrane guanylyl cyclase (GC), OlGC9, was identified in the intestine of the medaka fish Oryzias latipes by the isolation of a full-length cDNA clone (3783 bp).

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