Hokkaido University Collection of Scholarly and Academic Papers (52.405 recursos) HUSCAP (Hokkaido University Collection of Scholarly and Academic Papers) contains peer-reviewed journal articles, proceedings, educational resources and any kind of scholarly works of Hokkaido University.
Mostrando recursos 1 - 20 de 108
1. Effects of Overexpression and Antisense RNA Expression of Orf17, a MutT-Type Enzyme - Hori, Mika; Asanuma, Taketoshi; Inanami, Osamu; Kuwabara, Mikinori; Harashima, Hideyoshi; Kamiya, Hiroyuki The Escherichia coli Orf17 (NtpA, NudB) protein, a MutT-type enzyme, hydrolyzes oxidized deoxyribonucleotides, including 8-hydroxy-2'-deoxyadenosine 5'-triphosphate and 8-hydroxy-2'-deoxyguanosine 5'-triphosphate, in vitro.
2. Silencing of Exogenous DNA in Cultured Cells - Ochiai, Hiroshi; Harashima, Hideyoshi; Kamiya, Hiroyuki The intranuclear disposition of exogenous DNA is highly important for the therapeutic effects of the administrated DNA.
3. Genome Wide Expression Analysis of White Blood Cells and Liver of Pre-diabetic Otsuka Long-Evans Tokushima Fatty (OLETF) Rats Using a cDNA Microarray - Iida, Shinya; Sato, Yuichiro; Nakaya, Akihiro; Shinohara, Yasuo; Hayashi, Yasuhiro; Sawada, Akihiro; Nagata, Hideya; Kaji, Noritada; Kamiya, Hiroyuki; Baba, Yoshinobu; Harashima, Hideyoshi In a prior study, we reported on a significant decrease in calpain10 gene expression in white blood cells (WBC) as well as the major insulin-target tissues including liver and adipose tissue, before the onset of diabetes in Otsuka Long-Evans Tokushima Fatty (OLETF) rats.
7. Mutagenic effects of 8-hydroxy-dGTP in live mammalian cells - Satou, Kazuya; Kawai, Kazuaki; Kasai, Hiroshi; Harashima, Hideyoshi; Kamiya, Hiroyuki The mutagenicity of an oxidized form of dGTP, 8-hydroxy-2'-deoxyguanosine 5'-triphosphate (8-OH-dGTP), was examined using COS-7 cells.
8. Cell cycle dependent transcription, a determinant factor of heterogeneity in cationic lipid-mediated transgene expression. - Akita, Hidetaka; Ito, Rie; Kamiya, Hiroyuki; Kogure, Kentaro; Harashima, Hideyoshi Methods: To distinguish the contribution of nuclear transfer efficiency and subsequent intranuclear transcription efficiency to the overall heterogeneity in transgene expression, a novel imaging system was established for the dual visualization of the nuclear transfer of pDNA and marker gene expression (lacZ) in single cells.
9. Structure of Atg5.Atg16, a complex essential for autophagy. - Matsushita, Minako; Suzuki, Nobuo N.; Obara, Keisuke; Fujioka, Yuko; Ohsumi, Yoshinori; Inagaki, Fuyuhiko Atg5 is covalently modified with a ubiquitin-like modifier, Atg12, and the Atg12-Atg5 conjugate further forms a complex with the multimeric protein Atg16.
11. Regulation of STAT3-mediated signaling by LMW-DSP2. - Sekine, Y.; Tsuji, S.; Ikeda, O.; Sato, N.; Aoki, N.; Aoyama, K.; Sugiyama, K.; Matsuda, T. In this study, we examined whether low molecular weight-dual specificity phosphatase two (LMW-DSP2) is involved in the regulation of the interleukin 6 (IL-6)/leukemia inhibitory factor (LIF)/STAT3-mediated signaling pathway.
12. Physical and functional interactions between Daxx and STAT3. - Muromoto, R.; Nakao, K.; Watanabe, T.; Sato, N.; Sekine, Y.; Sugiyama, K.; Oritani, K.; Shimoda, K.; Matsuda, T. Signal transducer and activator of transcription 3 (STAT3) play key roles in the intracellular signaling pathways of the interleukin (IL)-6 family of cytokines, which exhibit a diverse set of cellular responses, including cell proliferation and differentiation.
15. Quantitative and mechanism-based investigation of post-nuclear delivery events between adenovirus and lipoplex. - Hama, Susumu; Akita, Hidetaka; Iida, Shinya; Mizuguchi, Hiroyuki; Harashima, Hideyoshi A previous quantitative comparison of intracellular trafficking between adenovirus and LipofectAMINE PLUS (LFN) revealed that the three orders of magnitude lower transfection efficiency of LFN was dominantly rate limited by the post-nuclear delivery process.
16. Practical Synthesis of Dirhodium (II) Tetrakis[N-phthaloyl-(S)-tert-leucinate] - TSUTSUI, Hideyuki; ABE, Takumi; NAKAMURA, Seiichi; ANADA, Masahiro; HASHIMOTO, Shunichi An efficient and reliable procedure for the preparation of dirhodium(II) tetrakis[N-phthaloyl-(S)-tert-leuci-nate], Rh_2(S-PTTL)_4, a universally effective catalyst for a range of enantioselective carbene transformations, is described.
17. Physical and functional interactions between STAP-2/BKS and STAT5. - Sekine, Yuichi; Yamamoto, Tetsuya; Yumioka, Taro; Sugiyama, Kenji; Tsuji, Satoshi; Oritani, Kenji; Shimoda, Kazuya; Minoguchi, Mayu; Yoshimura, Akihiko; Matsuda, Tadashi Signal-transducing adaptor protein family of proteins (STAPs), which currently contains two members, are proposed to be adaptor molecules because of their pleckstrin homology (PH) and Src-homology 2 (SH2)-like domains.
18. Regulation of transforming growth factor-? signaling by protein inhibitor of activated STAT, PIASy through Smad3. - Imoto, Seiyu; Sugiyama, Kenji; Muromoto, Ryuta; Sato, Noriko; Yamamoto, Tetsuya; Matsuda, Tadashi In this study we identified a protein inhibitor of activated STAT (signal transducers and activators of transcription), PIASy, as a novel interaction partner of Smad7 by yeast two-hybrid screening using the MH2 domain of Smad7 as bait.
20. Physical and functional interactions between STAT3 and ZIP kinase. - Sato, Noriko; Kawai, Taro; Sugiyama, Kenji; Muromoto, Ryuta; Imoto, Seiyu; Sekine, Yuichi; Ishida, Masato; Akira, Shizuo; Matsuda, Tadashi Signal transducer and activator of transcription 3 (STAT3) is a latent cytoplasmic transcription factor that can be activated by cytokines and growth factors.
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