Willcocks, Chris G.; Jackson, Philip T.G.; Nelson, Carl J.; Obara, Boguslaw
Helical objects occur in medicine, biology, cosmetics, nanotechnology, and engineering. Extracting a 3D parametric curve from a 2D image of a helical object has many practical applications, in particular being able to extract metrics such as tortuosity, frequency, and pitch. We present a method that is able to straighten the image object and derive a robust 3D helical curve from peaks in the object boundary. The algorithm has a small number of stable parameters that require little tuning, and the curve is validated against both synthetic and real-world data. The results show that the extracted 3D curve comes within close...
Nelson, Carl J.; Buckley, Charlotte; Mullins, John J.; Denvir, Martin A.; Taylor, Jonathan
How do you use imaging to analyse the development of the heart, which not only changes shape but also undergoes constant, high-speed, quasi-periodic changes? We have integrated ideas from prospective and retrospective optical gating to capture long-term, phase-locked developmental time-lapse videos. In this paper we demonstrate the success of this approach over a key developmental time period: heart looping, where large changes in heart shape prevent previous prospective gating approaches from capturing phase- locked videos. We use the comparison with other approaches to in vivo heart imaging to highlight the importance of collecting the most appropriate data for the biological...
Nelson, Carl j.; Duckney, Patrick; Hawkins, Timothy J.; Deeks, Michael J.; Laissue, P. Philippe; Hussey, Patrick J.; Boguslaw, Obara
Blobs and curves occur everywhere in plant bioimaging: from signals of fluorescence-labelled proteins, through cytoskeletal structures, nuclei staining and cell extensions such as root hairs. Here we look at the problem of colocalisation of blobs with blobs (protein-protein colocalisation) and blobs with curves (organelle-cytoskeleton colocalisation). This article demonstrates a clear quantitative alternative to pixel-based colocalisation methods and, using object-based methods, can quantify not only the level of colocalisation but also the distance between objects. Included in this report are computational algorithms, biological experiments and guidance for those looking to increase their use of computationally-based and quantified analysis of bioimages.
Płotka-Wasylka, Justyna; Simeonov, Vasil; Morrison, Calum; Namieśnik, Jacek
The demand for safer foods has promoted more research into biogenic amines (BAs) over the past few years, however, there are still some questions that remain unanswered. Despite the fact that BAs are present in wine and can cause toxic effect to the body, a shared regulation limiting the amounts of BAs in wine is still lacking. A detailed understanding of their presence in wine is also important for the food trade sector. Therefore, the aim of this work was to determine the level of selected BAs in wine samples origin from Poland. Thereafter, the evaluation of correlation between concentration...
Morgan, Ruth A.; Raftery, Alexandra G.; Cripps, Peter; Senior, Jonathan M.; McGowan, Catherine M.
The prevalence and nature of arrhythmias in horses following general anaesthesia and surgery is poorly documented. It has been proposed that horses undergoing emergency surgery for gastrointestinal disorders may be at particular risk of developing arrhythmias. Our primary objective was to determine the prevalence and nature of arrhythmias in horses following anaesthesia in a clinical setting and to establish if there was a difference in the prevalence of arrhythmias between horses with and without gastrointestinal disease undergoing surgery. Our secondary objective was to assess selected available risk factors for association with the development of arrhythmias following anaesthesia and surgery.
Raftery, Alexandra G.; Morgan, Ruth A.; MacFarlane, Paul D.
To compare perioperative trends in plasma colloid osmotic pressure (COP) between horses undergoing orthopedic and colic surgery.
Prospective clinical study September 2009–January 2011.
Veterinary university teaching hospital.
Thirty‐three healthy, client‐owned horses presenting for orthopedic surgery (non‐GI) and 85 client‐owned horses presenting for emergency exploratory celiotomy (GI, gastrointestinal).
Data relating to the horse's parameters on presentation, surgical lesion, post‐operative management and survival were extracted from computerized clinical records. Heparinized blood samples were taken on presentation (PreOp, pre‐operative), on recovery from anesthesia (T0), at 12 (T12) and 24 (T24) hours post recovery. COP was measured within 4 hours of collection.
Rajani, Rikesh M.; Quick, Sophie; Ruigrok, Silvie R.; Graham, Delyth; Harris, Sarah E.; Verhaaren, Benjamin F.J.; Fornage, Myriam; Seshadri, Sudha; Atanur, Santosh S.; Dominiczak, Anna F.; Smith, Colin; Wardlaw, Joanna M.; Williams, Anna
Dementia is a major social and economic problem for our aging population. One of the most common of dementia in the elderly is cerebral small vessel disease (SVD). Magnetic resonance scans of SVD patients typically show white matter abnormalities, but we do not understand the mechanistic pathological link between blood vessels and white matter myelin damage. Hypertension is suggested as the cause of sporadic SVD, but a recent alternative hypothesis invokes dysfunction of the blood-brain barrier as the primary cause. In a rat model of SVD, we show that endothelial cell (EC) dysfunction is the first change in development of...
Torrance, N.; Mansoor, R.; Wang, H.; Gilbert, S.; Macfarlane, G.J.; Serpell, M.; Baldacchino, A.; Hales, T.G.; Donnan, P.; Wyper, G.; Smith, B.H.; Colvin, L.
Opioid prescribing is increasing worldwide with associated increases in misuse and other harms. We studied variations in national opioid prescription rates, indicators of prescribing quality, co-prescribing of benzodiazepines and relationship with pain severity in Scotland.
Electronic linkages of opioid prescribing in Scotland were determined from: (i) national data from Information Services Division, NHS Scotland (2003–2012); and (ii) individual data from Generation Scotland: Scottish Family Health Study. Descriptive analyses were conducted on national data, multilevel modelling to examine factors associated with variations in prescribing rates. χ2 tests examined associations between individual pain severity and opioid prescriptions.
The number of strong...
Huang, Qi-Fang; Trenson, Sander; Zhang, Zhen-Yu; Van Keer, Jan; Van Aelst, Lucas N L; Yang, Wen-Yi; Nkuipou-Kenfack, Esther; Thijs, Lutgarde; Wei, Fang-Fei; Mujaj, Blerim; Ciarka, Agnieszka; Droogné, Walter; Vanhaecke, Johan; Janssens, Stefan; Van Cleemput, Johan; Mischak, Harald; Staessen, Jan A
Background: This proof-of-concept study investigated the feasibility of using biomarkers to monitor right heart pressures (RHP) in heart transplanted (HTx) patients.
Methods: In 298 patients, we measured 7.6 years post-HTx mean pressures in the right atrium (mRAP) and pulmonary artery (mPAP) and capillaries (mPCWP) along with plasma high-sensitivity troponin T (hsTnT), a marker of cardiomyocyte injury, and the multidimensional urinary classifiers HF1 and HF2, mainly consisting of dysregulated collagen fragments.
Results: In multivariable models, mRAP and mPAP increased with hsTnT (per 1-SD, +0.91 and +1.26 mm Hg; P < 0.0001) and with HF2 (+0.42 and +0.62 mm Hg; P ≤ 0.035), but...
Russell, Richard J; Chachi, Latifa; FitzGerald, J Mark; Backer, Vibeke; Olivenstein, Ronald; Titlestad, Ingrid L; Ulrik, Charlotte Suppli; Harrison, Timothy; Singh, Dave; Chaudhuri, Rekha; Leaker, Brian; McGarvey, Lorcan; Siddiqui, Salman; Wang, Millie; Braddock, Martin; Nordenmark, Lars H; Cohen, David; Parikh, Himanshu; Colice, Gene; Brightling, Christopher E
The role of interleukin 13 in airway inflammation and remodelling in asthma is unclear. Tralokinumab is a human monoclonal antibody that neutralises interleukin 13. We aimed to evaluate whether tralokinumab would have an effect on airway eosinophilic infiltration, blood and sputum eosinophil concentrations, eosinophil activation, and airway remodelling.
We did a multicentre, double-blind, randomised, placebo-controlled phase 2 trial at 15 centres across the UK, Denmark, and Canada. We enrolled participants of either sex aged 18–75 years with inadequately controlled moderate-to-severe asthma for 12 months or more, requiring treatment with inhaled corticosteroids at a stable dose. We randomly assigned participants...
Ginsberg, Henry N.; Farnier, Michel; Robinson, Jennifer G.; Cannon, Christopher P.; Sattar, Naveed; Baccara-Dinet, Marie T.; Letierce, Alexia; Bujas-Bobanovic, Maja; Louie, Michael J.; Colhoun, Helen M.
Diabetes mellitus (DM) carries an elevated risk for cardiovascular disease. Here, we assessed alirocumab efficacy and safety in people with/without DM from five placebo-controlled phase 3 studies.
Data from up to 78 weeks were analyzed in individuals on maximally tolerated background statin. In three studies, alirocumab 75 mg every 2 weeks (Q2W) was increased to 150 mg Q2W at week 12 if week 8 low-density lipoprotein cholesterol (LDL-C) was ≥ 70 mg/dL; two studies used alirocumab 150 mg Q2W throughout. The primary endpoint was percentage change in LDL-C from baseline to week 24.
In the alirocumab 150 mg pool (n = 2416),...
Schmitz, Lutz; Gupta, Girish; Segert, Marc Hanno; Kost, Ricarda; Sternberg, Julia; Gambichler, Thilo; Stockfleth, Eggert; Dirschka, Thomas
Background/Aims: Actinic keratosis area and severity index
(AKASI) is a new assessment tool to quantify the severity of
actinic damage on the head. Thus far, it has not been evaluated
in monitoring the efficacy of field-directed topical treatments
in actinic keratosis (AK) in routine clinical practice.
Thus, the aim of this study was to determine treatment outcomes
by using AKASI 3 months after the initiation of topical
application of diclofenac sodium 3% in hyaluronic acid 2.5%
gel (DFS) in patients with AKs on the head. Methods: We performed
a retrospective analysis of patients with AKs who had
AKASI scores prior to and after treatment with DFS. Results:
Of the 24 patients...
Wilson, Duncan; Ambler, Gareth; Shakeshaft, Clare; Brown, Martin M; Charidimou, Andreas; Al-Shahi Salman, Rustam; Lip, Gregory Y H; Cohen, Hannah; Banerjee, Gargi; Houlden, Henry; White, Mark J; Yousry, Tarek A; Harkness, Kirsty; Flossmann, Enrico; Smyth, Nigel; Shaw, Louise J; Warburton, Elizabeth; Muir, Keith W.; Jäger, Hans Rolf; Werring, David J
Cerebral microbleeds are a potential neuroimaging biomarker of cerebral small vessel diseases that are prone to intracranial bleeding. We aimed to determine whether presence of cerebral microbleeds can identify patients at high risk of symptomatic intracranial haemorrhage when anticoagulated for atrial fibrillation after recent ischaemic stroke or transient ischaemic attack.
Our observational, multicentre, prospective inception cohort study recruited adults aged 18 years or older from 79 hospitals in the UK and one in the Netherlands with atrial fibrillation and recent acute ischaemic stroke or transient ischaemic attack, treated with a vitamin K antagonist or direct oral anticoagulant, and followed...
Khajehali, Elham; Valant, Celine; Jörg, Manuela; Tobin, Andrew B.; Conn, P. Jeffrey; Lindsley, Craig W.; Sexton, Patrick M.; Scammells, Peter J.; Christopoulos, Arthur
Subtype-selective allosteric modulation of the M1 muscarinic acetylcholine (ACh) receptor (M1 mAChR) is an attractive approach for the treatment of numerous disorders, including cognitive deficits. The discovery of benzyl quinolone carboxylic acid, BQCA, a selective M1 mAChR positive allosteric modulator (PAM), spurred the subsequent development of newer generation M1 PAMs representing diverse chemical scaffolds, different pharmacodynamic properties and, in some instances, improved pharmacokinetics. Key exemplar molecules from such efforts include PF-06767832 (N-((3R,4S)-3-hydroxytetrahydro-2H-pyran-4-yl)-5-methyl-4-(4-(thiazol-4-yl)benzyl)pyridine-2-carboxamide), VU6004256 (4,6-difluoro-N-(1S,2S)-2-hydroxycyclohexyl-1-((6-(1-methyl-1H-pyrazol-4-yl)pyridine-3-yl)methyl)-1H-indole-3-carboxamide) and MIPS1780 (3-(2-hydroxycyclohexyl)-6-(2-((4-(1-methyl-1H-pyrazol-4-yl)-benzyl)oxy)phenyl)pyrimidin-4(3H)-one). Given these diverse scaffolds and pharmacodynamics, the current study combined pharmacological analysis and site-directed mutagenesis to explore the potential binding site and function...
Colombo, Marco; Looker, Helen C.; Farran, Bassam; Agakov, Felix; Brosnan, M.Julia; Welsh, Paul; Sattar, Naveed; Livingstone, Shona; Durrington, Paul N.; Betteridge, D.John; McKeigue, Paul M.; Colhoun, Helen M.
Background and aims:
Developing sparse panels of biomarkers for cardiovascular disease in type 2 diabetes would enable risk stratification for clinical decision making and selection into clinical trials. We examined the individual and joint performance of five candidate biomarkers for incident cardiovascular disease (CVD) in type 2 diabetes that an earlier discovery study had yielded.
Apolipoprotein CIII (apoCIII), N-terminal prohormone B-type natriuretic peptide (NT-proBNP), high sensitivity Troponin T (hsTnT), Interleukin-6, and Interleukin-15 were measured in baseline serum samples from the Collaborative Atorvastatin Diabetes trial (CARDS) of atorvastatin versus placebo. Among 2105 persons with type 2 diabetes and median age of...
Shah, Dhvani; Driessen, Maurice; Risebrough, Nancy; Baker, Timothy; Naya, Ian; Briggs, Andrew; Ismaila, Afisi S.
Cost-effectiveness of once-daily umeclidinium bromide (UMEC) was compared with once-daily tiotropium (TIO) and once-daily glycopyrronium (GLY) in patients with chronic obstructive pulmonary disease (COPD) from a UK National Health Service (NHS) perspective.
A linked-equation model was implemented to estimate COPD progression, associated healthcare costs, exacerbations rates, life years (LY) and quality-adjusted LY (QALYs). Statistical risk equations for endpoints and resource use were derived from the ECLIPSE and TORCH studies, respectively. Treatment effects [mean (standard error)] at 12 weeks on forced expiratory volume in 1 s and St George’s Respiratory Questionnaire score were obtained from the intention-to-treat populations of two...
Root, Amanda L.; Parkin, Tim D.; Hutchison, Pippa; Warnes, Caroline; Yam, Philippa S.
Background: There is a dearth of literature on pseudopregnancy in the bitch, with only a few treatment-based
studies published since the 1990s. Pseudopregnancy may be under-recognised in bitches and may account for a
proportion of behavioural cases seen in veterinary practices including aggression. Little is known about commonly
used treatments for overtly pseudopregnant bitches and it is possible that current regimes may not be prescribed
for a sufficient duration to control any clinical signs including, physical and behavioural changes. To investigate
current trends in diagnosis and treatment of canine pseudopregnancy, a postal survey was sent to 2000 randomly
selected veterinary surgeons in UK veterinary practices. The...
Lochard, G.; Schlesinger, P.
No abstract available.
No abstract available.
The University of Glasgow’s institutional repository, Enlighten, has been providing open access to researcher’s outputs since 2002 through our Eprints platform of Enlighten Open services. As the landscape of open access has progressed over the last 10 years, the services and support offered to academic staff has grown. In 2007 there were 5 members of staff involved in supporting Enlighten with 443 records deposited compared to 2017 with 12,866 records deposited and 15 staff regularly working with Enlighten.
The Enlighten team now regularly add records on behalf of academic staff from various sources such as author lists, ORCID and database...